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Purpose: To determine the effect of lens status (pseudophakic vs phakic) on diabetic vitreous hemorrhage (VH) resolution time. Methods: Medical records were reviewed retrospectively for each case of diabetic VH until resolution, pars plana vitrectomy (PPV), or loss to follow-up. Univariate and multivariate Cox regression models were used to determine predictors of diabetic VH resolution time through estimated hazard ratios (HRs). Kaplan-Meier survival analysis compared differences in the resolution rate by lens status and other significant factors. Results: Overall, 243 eyes were included. Pseudophakia (HR, 1.76; 95% CI, 1.07-2.90; P = .03) and prior PPV (HR, 3.28; 95% CI, 1.77-6.07; P

Local field potentials (LFPs) represent the summation of periodic (oscillations) and aperiodic (fractal) signals. Although previous studies showed changes in beta band oscillations and burst characteristics of the subthalamic nucleus (STN) in Parkinson's disease (PD), how aperiodic activity in the STN is related to PD pathophysiology is unknown.The study aimed to characterize the long-term effects of STN-deep brain stimulation (DBS) and dopaminergic medications on aperiodic activities and beta bursts.A total of 10 patients with PD participated in this longitudinal study. Simultaneous bilateral STN-LFP recordings were conducted in six separate visits during a period of 18 months using the Activa PC + S device in the off and on dopaminergic medication states. We used irregular-resampling auto-spectral analysis to separate oscillations and aperiodic components (exponent and offset) in the power spectrum of STN-LFP signals in beta band.Our results revealed a systematic increase in both the exponent and the offset of the aperiodic spectrum over 18 months following the DBS implantation, independent of the dopaminergic medication state of patients with PD. In contrast, beta burst durations and amplitudes were stable over time and were suppressed by dopaminergic medications.These findings indicate that oscillations and aperiodic activities reflect at least partially distinct yet complementary neural mechanisms, which should be considered in the design of robust biomarkers to optimize adaptive DBS. Given the link between increased gamma-aminobutyric acidergic (GABAergic) transmission and higher aperiodic activity, our findings suggest that long-term STN-DBS may relate to increased inhibition in the basal ganglia. © 2022 International Parkinson and Movement Disorder Society.

Direct canopy uptake of atmospheric reactive nitrogen (ARN) is an important process, but the magnitude of ARN assimilation in agricultural ecosystems is unclear. We used a combination of a water-culture rice-growing system with a

Self-templating is a facile strategy for synthesizing porous carbons by direct pyrolysis of organic metal salts. However, the method typically suffers from low yields (< 4%) and limited specific surface areas (SSA < 2000 m2 g-1) originating from low activity of metal cations (e.g., K+ or Na+) in promoting construction and activation of carbon frameworks. Here we use cesium acetate as the only precursor of oxo-carbons with large SSA of the order of 3000 m2 g-1, pore volume approaching 2 cm3·g-1, tunable oxygen contents, and yields of up to 15%. We unravel the role of Cs+ as an efficient promoter of framework formation, templating and etching agent, while acetates act as carbon/oxygen sources of carbonaceous frameworks. The oxo-carbons show record-high CO2 uptake of 8.71 mmol·g-1 and an ultimate specific capacitance of 313 F·g-1 in the supercapacitor.This study helps to understand and rationally tailor the materials design by a still rare organic solid-state chemistry.

Van Gogh-like 2 (Vangl2), a core planar cell polarity (PCP) component, plays an important role in polarized cellular and tissue morphology induction, growth development and cancer. However, its role in regulating inflammatory responses remains elusive. Here, we report that Vangl2 is upregulated in patients with sepsis and identify Vangl2 as a negative regulator of NF-κB signaling by regulating the protein stability and activation of the core transcription component p65. Mice with myeloid-specific deletion of Vangl2 (Vangl2ΔM) are hypersusceptible to lipopolysaccharide (LPS)-induced septic shock. Vangl2 deficient myeloid cells exhibit enhanced phosphorylation and expression of p65, therefore, promoting the secretion of pro-inflammatory cytokines after LPS stimulation. Mechanistically, NF-κB signaling-induced-Vangl2 recruits E3 ubiquitin ligase PDLIM2 to catalyze K63-linked ubiquitination on p65, which serves as a recognition signal for cargo receptor NDP52-mediated selective autophagic degradation. Taken together, these findings demonstrate Vangl2 as a suppressor of NF-κB mediated inflammation and provide insights into the crosstalk between autophagy and inflammatory diseases.

Background: Knotted suture bridge repair (KSBR) has been widely proven to be an effective method for rotator cuff repairs. However, the occurrence of type 2 failure after suture bridge repair remains a frequent problem because of the stress concentration and disturbance of tendon perfusion in the medial row. The authors have developed the H-loop knotless double–row repair (HLDR) to counteract these problems. Purpose: To compare the biomechanical and histological outcomes of HLDR and KSBR for rotator cuff tear in the rabbit model. Study Design: Controlled laboratory study. Methods: Acute bilateral supraspinatus tears were created on the shoulders of 46 New Zealand White rabbits. HLDR and KSBR were randomly performed on the left side or right side. Thirteen animals each were sacrificed at 2, 4, and 8 weeks after surgery (n = 39), with 6 rabbits used for histological evaluation and the other 7 rabbits for biomechanical testing. The remaining 7 animals from the original 46 were only used for initial biomechanical evaluation at week 0. Results: Macroscopically, all repaired tendons were connected to their footprint on the greater tuberosity without postoperative complications at 8 weeks after surgery. The HLDR group had significantly better histological bone–to–tendon integration compared with the KSBR group in terms of fibrocartilage regeneration, collagen composition, and fiber organization. The biomechanical outcomes in the HLDR group were demonstrated to be better than those of the KSBR group at time 0 and 8 weeks after surgery. Conclusion: Both repair techniques were effective for rotator cuff tears in a rabbit rotator cuff tear model; however, HLDR demonstrated more advantages in improving biomechanical properties and histological tendon–to–bone healing compared with KSBR. Clinical Relevance: This animal study suggested that HLDR might be an alternative choice for rotator cuff tears in humans to increase tendon–to–bone healing and reduce the rate of failure to heal.

Ke Zeng,1 Jun Huang,2 Xiaobin Li,2 Junbing Ye,2 Jian Yang,2 Suchuan Zhang3 1Department of Basic Medicine, Sichuan Vocational College of Health and Rehabilitation, Zigong, Sichuan, People’s Republic of China; 2Department of Urology, First People’s Hospital of Zigong City, Zigong, Sichuan, People’s Republic of China; 3Laboratory Department, First People’s Hospital of Zigong City, Zigong, Sichuan, People’s Republic of ChinaCorrespondence: Suchuan Zhang, Laboratory Department, First People’s Hospital of Zigong City, No. 42, Shangyihao No.1 Road, Ziliujing District, Zigong, Sichuan, People’s Republic of China, Email suche89103627975@126.comObjective: To analyze the distribution and drug resistance of pathogenic bacteria in diabetic patients with double J-stent associated infections, and to explore the strategies for prevention and treatment of the infections.Methods: From January 2019 to December 2021, 266 diabetic patients treated with double J-stent placement in our hospital assessed for eligibility were recruited. Urine and double J-stent samples were collected for pathogenicity assay and screened for biofilm bacteria. Pathogenic bacteria distribution and drug resistance were examined.Results: A total of 97 strains (36.5%) of pathogenic bacteria were isolated from urine samples and 129 strains (48.5%) from double J-stent samples (P > 0.05). 3 strains (1.1%) of biofilm bacteria were separated from urine samples and 106 strains (39.8%) from double J-stent samples (P < 0.05). In the double J-stent samples, there were significantly higher ratios of Gram-positive bacteria separated from biofilm bacteria versus the urine-cultured pathogens (44.3%/61.3%, P < 0.05), and higher drug resistance was observed in biofilm bacteria versus urine-cultured pathogens (P < 0.05). Fosfomycin tromethamine showed remarkable susceptibility to both urinary cultured pathogens and double J-stent biofilm bacteria.Conclusion: Diabetic patients with double J-stent biofilm-positive bacteria are mainly Gram-positive bacteria, which are prone to biofilm formation and show strong drug resistance.Keywords: pathogenic bacteria distribution, drug resistance, diabetic patients, double J-stent

The meta- and para-catenated methylene-containing phthalonitrile oligomers were prepared from the reaction of an excess amount of 4,4′-(4,4′-isopropylidenediphenoxy) bis-(phthalic anhydride) (BPADA) with 4,4′-Methylenedianiline (MDA) in a N, N-dimethylformamide/ toluene solvent mixture, followed by end-capping agent with 4-nitrophthalonitrile or 4-phenoxyaniline in a two-step, one-pot reaction. Differential scanning calorimetry (DSC) showed that both PN oligomers exhibited low softening points. The self-catalyzed curing reactivity of the PN oligomers was confirmed by the isothermal rheological measurements. Fourier transform infrared spectroscopy (FTIR) and ultraviolet and visible spectrophotometry (UV–Vis) data of the pre-curing resins were employed to investigate the chemical structure of the pre-cured resins, suggesting that oligomers generated crosslinking sites, including triazine, isoindoline, and phthalocyanine. The results further confirmed the self-catalyzed curing reactivity of the oligomers. Thermal properties were investigated by dynamic mechanical analysis (DMA) and thermal gravimetric analysis (TGA), demonstrating good thermal properties of the cured resins. The glass transition temperatures (Tgs) of PIPN-1-325, PIPN-1-350, PIPN-1-375 were in the range of 285–345°C, the 5% weight loss temperature (T5%) was observed at 482°C. The PIPN-2-325, PIPN-2-350, PIPN-2-375 showed Tgs ranging from 293 to 370°C, and T5% of the resins were in the range of 481–501°C. Then the isothermal rheological results of model compound and PN oligomers implied that the curing process of PN oligomers was closely related to the methylene-cyano radical thermal synergistic polymerization (TSP) effect proposed in our previous research, and then a revised curing mechanism (radical TSP mechanism) was proposed.

A series of adenine-containing polyimides (APIs) with intramolecular hydrogen bonding (HB) were prepared from N 1 -(9-(4-aminophenyl)- 9H-purin-6-yl)-2-methoxybenzene-1,4-diamine (b-H-MeO-APA) and commercial dianhydrides. And a systematic comparison between intramolecular and intermolecular HB effects was made using APIs with (b-H-MeO-APIs) and without (b-H-H-API) methoxy group. FTIR indicated that the occurrence of intramolecular HB originated from the side MeO group ortho to -NH-. XRD indicated that the introduction of side group MeO and corresponding intramolecular HB could destroy the orderly stacking of b-H-H-APIs. Birefringence and polarized ATR FTIR studies showed that the groups and chains in b-H-MeO-APIs films could also be simultaneously self-arranged in the in-plane direction. In contrast, the orientation degree in the in-plane direction is generally lower than that of b-H-H-APIs. The b-H-MeO-APIs films showed lower water absorption, higher Tg, and lower CTE compared to b-H-H-APIs films, which indicates that the introduction of intramolecular HB may have some positive influence in controlling water absorption, Tg, and CTE.

High switching temperature shape memory polymers were developed from copolyimides via a facile random copolymerization strategy.

Objective: To analyze the distribution and drug resistance of biofilm bacteria infected with upper urinary calculi patients with double J stent indwelling and its influencing factors. Methods: A total of 400 patients with upper urinary calculi who adopted double J stent inserting in our hospital from January 2019 to January 2022 were included. The urine and double J stent samples were collected and pathogen culture were performed, and the drug sensitivity test analysis were carried out for isolates.Univariate and multivariate logistic regression analyses were used to analyze the influencing factors of patients with double J-stent associated biofilm bacteria infections. Results: A total of 13 strains (3.2%) of biofilm bacteria were detected from urine samples and 168 strains (42%) from double J-stent samples (P < 0.05), 95 strains (23.7%) of pathogenic bacteria were separated from urine samples and 117 strains (29.2%) from double J-stent samples (P > 0.05). Escherichia coliwere the most common bacteria. there were significantly higher drug resistance was observed in biofilm bacteria versus urine-cultured pathogens (P < 0.05). Advanced age, long-term catheterization, insufficient water intake, hypoproteinemia, abnormal renal function and diabetes mellitus were independent risk factors for biofilm bacteria infection associated with double J stent(P < 0.05). Conclusion: Among the upper urinary calculi patients with double J stent indwelling, the positive rate and drug resistance of biofilm bacteria obtained from double-J stent were significantly higher than that from urine.Deem that rational choice of antibiotics and appropriate treatment led to better prognosis.

Radiofrequency ablation and cryoballoon ablation are the main methods of catheter ablation for patients with atrial fibrillation (AF). However, different ablation catheters have different efficacy and complication rates. This study aimed to compare the efficacy and complication rates of quantitative radiofrequency ablation guided by ablation index (RFCA-AI) with those of second-generation cryoballoon ablation (CBA-2).A total of 230 consecutive patients with symptomatic AF undergoing a first ablation procedure of CBA-2 (92 patients) or RFCA-AI (138 patients) were enrolled in this study.The late recurrence rate in the CBA-2 group was higher than that in the RFCA-AI group (p = 0.012). Subgroup analysis showed the same result in patients with paroxysmal AF (PAF) (p = 0.039), but no difference was found in patients with persistent AF (p = 0.21). The average operation duration in the CBA-2 group (85 (75-99.5) min) was shorter than that in the RFCA-AI group (100 (84.5-120) min), but the average exposure time in the CBA-2 group (17.36 (13.87-22.49) min vs 5.49 (4.00-8.24) min; p0.0001) and X-ray dose (223.25 (149.15-336.95) min vs 109.15 (80.75-168.7) min; p0.0001) were significantly longer than those in RFCA-AI group. The patients were then divided into a late recurrent group and a late recurrence-free group. Multivariate logistic regression analysis showed that left atrial diameter (LAD), early recurrence, and methods of ablation (cryoballoon ablation) were independent risk factors for late recurrence after AF ablation.For patients with PAF, CBA-2 was inferior to RFCA-AI. Furthermore, although RFCA-AI had an equivalent complication rate and longer operation time than CBA-2, it also had a shorter exposure time and required a lower radiation dose. The early recurrence of AF and LAD were independent risk factors for predicting late recurrence after AF ablation (Tab. 5, Fig. 3, Ref. 29).

The tumor microenvironment (TME) featured by immunosuppression and hypoxia is pivotal to cancer deterioration and metastasis. Thus, regulating the TME to improve cancer cell ablation efficiency has received extensive interest in oncotherapy. However, to reverse the immunosuppression and alleviate hypoxia simultaneously in the TME are major challenges for effective cancer therapy. Herein, a multifunctional platform based on Au nanoparticles and a carbon dots modified hollow black TiO

Although previous studies have focused on the associations between peer group relations and cyber-aggression, limited attention has been paid to the heterogeneity in the functions of cyber-aggression. This study explored the unique associations of peer relations with proactive and reactive cyber-aggression and the possible mechanisms underlying them in a sample of adolescents using a longitudinal study design. A total of 829 middle school students completed the Cyber-rage and Cyber-reward Aggression Subscales of the Cyber-Aggression Typology Questionnaire, the Peer Relations Scale, the Social Information Processing-Attributional Bias Questionnaire, and the Self-efficacy for Aggression Scale twice at a 6-month interval. Multiple mediation analyses and bootstrapping were conducted using the Mplus 8 software. The results indicated that satisfying peer relations were negatively correlated with reactive cyber-aggression and positively associated with proactive cyber-aggression. Moreover, hostile intent attribution and self-efficacy for aggression mediated the associations between peer relations and both functions of cyber-aggression, however, in different ways. Unsatisfying peer relations were associated with higher levels of hostile intent attribution and lower levels of self-efficacy for aggression and predicted increases in reactive cyber-aggression. In contrast, satisfying peer relations were associated with lower levels of hostile intent attribution and higher levels of self-efficacy for aggression and predicted increases in proactive cyber-aggression. The findings indicated that different functions of cyber-aggression might be related to different mediation mechanisms, which sheds light on the prevention of cyber-aggression in the future.

Ke Zeng,1,&ast; Jia-Mo Zhang,2,&ast; Xiao-Bin Li,1 Sheng-Xian Peng,3 Su-Chuan Zhang,4 Wen-Xian Xie,5 Chun-Fang Xi,1 Cheng-Jian Cao6 1Department of Urology, First People’s Hospital of Zigong City, Zigong, People’s Republic of China; 2Department of Urology, YongChuan Hospital Chongqing Medical University, Chongqing, People’s Republic of China; 3Scientific Research Department, First People’s Hospital of Zigong City, Zigong, People’s Republic of China; 4Laboratory Department, First People’s Hospital of Zigong City, Zigong, People’s Republic of China; 5Department of Basic Medicine, Sichuan Vocational College of Health and Rehabilitation, Zigong, People’s Republic of China; 6Zigong Academy of Medical Sciences, First People’s Hospital of Zigong City, Zigong, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Cheng-Jian CaoZigong Academy of Medical Sciences, First People’s Hospital of Zigong City, Zigong, People’s Republic of ChinaEmail cccjj2011@163.comObjective: To analyze the bacterial biofilm (BF) formation in patients with malignancy undergoing double J stent indwelling and its influencing factors.Methods: A total of 167 patients with malignant tumors who received double J stent indwelling in the hospital from January 2018 to January 2021 were included in the study. The urine and double J stent samples were collected for bacterial identification and observed for BF formation on the surface of the urinary catheter under a scanning electron microscope (SEM). Univariate and multivariate logistic regression analyses were used to analyze the influencing factors of BF.Results: The BF formation rate was 34.73% (58/167). The BF formation rate of positive specimens cultured in urine and double J stent was significantly higher than that of negative ones (P< 0.05). Staphylococcus was the main BF bacteria in double J stent and urine culture specimens, followed by Enterococcus, Pseudomonas, Enterobacter, and Acinetobacter. Compared with the non-BF group, the number of viable bacteria in the double J stent and urine and the catheterization time in the BF group rose markedly (P< 0.05). Advanced age, chemotherapy, anemia, indwelling time ≥ 90d, and urinary tract infection were risk factors for BF formation in patients with malignancy undergoing double J stent indwelling (P< 0.05).Conclusion: There is a high rate of BF formation in patients with malignancy undergoing double J stent indwelling, with Staphylococcus as the dominant species. Treatment requires enhanced urinary catheter management and nutritional status to inhibit BF formation and lower the rate of urinary catheter-related infections.Keywords: malignant tumor, double J stent indwelling, biofilm, catheter-related infection, etiology, influence factor

As a key component of innate immunity, inflammasome is critical for host antitoxoplasmosis immunity, but the underlying mechanisms are still elusive. In this study, we found that inflammasome signaling was activated by PAMPs of T. gondii , which generated a protective immunity against T. gondii invasion by suppressing type I interferon (IFN-I) production. Mechanically, inflammasome-coupled IL-1β signaling triggered the expression of negative regulator SOCS1, which bound to IRF3 to inhibit IFN-I production.

Background Perivascular epithelioid cell tumor (PEComa) is a mesenchymal tumor with distinct histologic and immunologic features. PEComas that originate in the bladder are extremely rare clinically, with only 35 cases reported in the English literature thus far. Here, we report a case of bladder PEComa resection by transurethral en bloc resection of bladder tumor (ERBT). Case presentation A 66-year-old female with a history of poorly controlled type 2 diabetes with associated complications of frequent urinary tract infections presented to our hospital for a routine physical examination. Outpatient ultrasound examination revealed a strong echogenic mass of approximately 1.5 × 1.3 × 1.3 cm in size on the posterior wall of the bladder. The enhanced computed tomography and enhanced magnetic resonance imaging after admission both suggested a well-defined isolated nodular mass on the posterior wall of the bladder with significant enhancement on the enhanced scan. The tumor was successfully and completely resected by ERBT. Postoperative pathological examination and immunohistochemical results confirmed the mass was a bladder PEComa. No tumor recurrence was observed in the six-month postoperative follow-up. Conclusion Bladder PEComa is an extremely rare mesenchymal tumor of the urinary system. When imaging and cystoscopy reveal a nodular mass with an abundant blood supply in the bladder, PEComa should be included in the differential diagnosis of bladder tumors. Surgical resection is currently the primary option for the treatment of bladder PEComa. For a solitary, pedunculated, narrow-based, small-sized bladder PEComa, resection of the tumor by ERBT was a safe and feasible approach in our patient and may be considered for similar cases in the future.

Innate immunity acts as the first line of defense against pathogen invasion. During Toxoplasma gondii infection, multiple innate immune sensors are activated by invading microbes or pathogen-associated molecular patterns (PAMPs). However, how inflammasome is activated and its regulatory mechanisms during T. gondii infection remain elusive. Here, we showed that the infection of PRU, a lethal type II T. gondii strain, activates inflammasome at the early stage of infection. PRU tachyzoites, RNA and soluble tachyzoite antigen (STAg) mainly triggered the NLRP3 inflammasome, while PRU genomic DNA (gDNA) specially activated the AIM2 inflammasome. Furthermore, mice deficient in AIM2, NLRP3, or caspase-1/11 were more susceptible to T. gondii PRU infection, and the ablation of inflammasome signaling impaired antitoxoplasmosis immune responses by enhancing type I interferon (IFN-I) production. Blockage of IFN-I receptor fulfilled inflammasome-deficient mice competent immune responses as WT mice. Moreover, we have identified that the suppressor of cytokine signaling 1 (SOCS1) is a key negative regulator induced by inflammasome-activated IL-1β signaling and inhibits IFN-I production by targeting interferon regulatory factor 3 (IRF3). In general, our study defines a novel protective role of inflammasome activation during toxoplasmosis and identifies a critical regulatory mechanism of the cross talk between inflammasome and IFN-I signaling for understanding infectious diseases.

Synovial macrophage polarization and inflammation are essential for osteoarthritis (OA) development, yet the molecular mechanisms and regulation responsible for the pathogenesis are still poorly understood. Here, we report that pseudolaric acid B (PAB) attenuated articular cartilage degeneration and synovitis during OA. PAB, a diterpene acid, specifically inhibited NF‐κB signalling and reduced the production of pro‐inflammatory cytokines, which further decreased M1 polarization and vessel formation. We further provide in vivo and in vitro evidences that PAB suppressed NF‐κB signalling by stabilizing PPARγ. Using PPARγ antagonist could abolish anti‐inflammatory effect of PAB and rescue the activation of NF‐κB signalling during OA. Our findings identify a previously unrecognized role of PAB in the regulation of OA and provide mechanisms by which PAB regulates NF‐κB signalling through PPARγ, which further suggest targeting synovial inflammation or inhibiting vessel formation at early stage could be an effective preventive strategy for OA.

Previous studies have shown that the brain generates expectations based on scenes, which affects facial expression recognition. However, although facial expressions are known to interact with perception, the mechanism underlying this interaction remains poorly understood. Here, we used frequency labeling and decoding techniques to reveal the effects of scene-based expectation on the amplitude and representational strength of neural activity. We also reduced the relative reliability between expectation and sensory input by blurring facial expressions to further investigate the effects of this relative reliability on the pattern of neural activation and representation. Participants viewed emotional changes in unblurred or blurred facial expressions, which flickered at a rate of 6 Hz within a scene. We found that facial expressions that were congruent with the emotional significance of the scene elicited a larger steady-state visual evoked potential amplitude than did facial expressions that were incongruent with the emotional significance of a scene, in both unblurred and blurred conditions. We also found that expected facial expression representations were stronger than unexpected representations during the unblurred condition. In the blurred condition, unexpected representations were stronger than expected representations. Taken together, these results suggested that facial expression processing in the visual cortex is modulated by top-down signals. The relative reliability of expectation and sensory input moderated the influence of a scene on facial expression representation. Furthermore, our study showed that neural activation amplitudes did not correspond to representational strength.

Alloy-type anodes are one of the most promising classes of next-generation anode materials due to their ultrahigh theoretical capacity (2-10 times that of graphite). However, current alloy-type anodes have several limitations: huge volume expansion, high tendency to fracture and disintegrate, an unstable solid-electrolyte interphase (SEI) layer, and low Coulombic efficiency. Efforts to overcome these challenges are ongoing. This Review details recent progress in the research of batteries based on alloy-type anodes and discusses the direction of their future development. We conclude that improvements in structural design, the introduction of a protective interface, and the selection of suitable electrolytes are the most effective ways to improve the performance of alloy-type anodes. Furthermore, future studies should direct more attention toward analyzing their synergistic promoting effect.

Enteral feeding tubes are used in neonatal intensive care units (NICUs) to assess feeding tolerance by utilizing preprandial gastric residual aspiration. This study evaluates the effect of gastric residual aspiration on the preterm infant fecal microbiome and gastrointestinal inflammation. Fifty-one very low birth weight (VLBW) infants (≤32 weeks' gestational age and ≤1,250 g) enrolled in a larger single-center randomized controlled trial evaluating the effects of routine and nonroutine gastric residual aspiration were selected for further analysis. Of those infants, 30 had microbiome analysis performed on stools collected at 6 weeks by sequencing the bacterial V1 to V3 variable regions of the genes encoding for 16S rRNA. In an additional 21 infants, stool samples collected at 3 and 6 weeks were analyzed for intestinal inflammation using a cytokine multiplex panel. Microbial communities between groups were not distinct from each other and there was no difference in intestinal inflammation between groups. Analyses using gene expression packages DESeq2 and edgeR produced statistically significant differences in several taxa, possibly indicating a more commensal intestinal microbiome in infants not undergoing gastric residual aspiration. Omission of routine gastric residual aspiration was not associated with intestinal dysbiosis or inflammation, providing additional evidence that monitors preprandial gastric residuals is unnecessary.· Omission of routine gastric residual aspiration was not associated with intestinal dysbiosis or inflammation.. · Existing literature indicates preprandial gastric aspiration does not reliably correlate with development of necrotizing enterocolitis but does correlate with delayed enteral nutrition.. · Further study is required but this data that suggest monitoring preprandial gastric residuals are unnecessary..

CD4Ex vivo-expanded UCB Tregs exhibited a unique phenotype with co-expression of CD45RAUCB Treg adoptive therapy is a promising therapeutic strategy for treatment of lung injury.