1. Single step isolation and activation of primary CD3+ T lymphocytes using alcohol-dispersed electrospun magnetic nanofibers
- Author
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Kim, Kwanghee, An, Hyo Jin, Jun, Seung-Hyun, Kim, Tae-Jin, Lim, Seon Ah, Park, Gayoung, Na, Hyon Bin, Park, Yong Il, Hyeon, Taeghwan, Yee, Cassian, Bluestone, Jeffrey A, Kim, Jungbae, and Lee, Kyung-Mi
- Subjects
CD3 Complex ,T-Lymphocytes ,Nanofibers ,CD4(+) T lymphocytes ,Bioengineering ,Cell Separation ,Inbred C57BL ,Lymphocyte Activation ,Antibodies ,Mice ,Electrospun magnetic nanofiber ,anti-CD3 monoclonal antibody ,Alcohols ,CD8(+) T lymphocytes ,Monoclonal ,Animals ,Nanotechnology ,Nanoscience & Nanotechnology ,Magnetite Nanoparticles ,immune cell therapy ,Biotechnology - Abstract
Electrospun polymer nanofibers with entrapped magnetic nanoparticles (magnetic NP-NF) represent a novel scaffold substrate that can be functionalized for single-step isolation and activation of specific lymphocyte subsets. Using a surface-embedded T cell receptor ligand/trigger (anti-CD3 monoclonal antibody), we demonstrate, as proof of principle, the use of magnetic NP-NF to specifically isolate, enrich, and activate CD3(+) T cells from a heterogeneous cell mixture, leading to preferential expansion of CD8(+)CD3(+) T cells. The large surface area, adjustable antibody density, and embedded paramagnetic properties of the NP-NF permitted enhanced activation and expansion; its use represents a strategy that is amenable to an efficient selection process for adoptive cellular therapy as well as for the isolation of other cellular subsets for downstream translational applications.
- Published
- 2012