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BackgroundLarge distribution of long-lasting insecticidal nets (LLINs) and indoor residual spraying (IRS) contributed to a significant decrease in malarial mortality. Unfortunately, large insecticide resistance in malaria vectors occurred and is a threat to the future use of these control approaches. The purpose of this study was to explore a new approach for vector control. Patches containing Bacillus thuringiensis var. israelensis (Bti) solubilized Cry toxins mixed with sugar were developed and tested in the laboratory with pyrethroid-resistant Anopheles gambiae s.l. using tunnel tests.MethodsMosquitoes were released at 6:00 p.m. into a large tunnel separated by a bed net, perforated with nine holes, from a smaller chamber with a guinea pig. Nine Bti sugar patches (BSPs) were attached to the bed net between the nine holes. Fourteen hours later (8:00 a.m.), mosquitoes were collected from the tunnel and the guinea pig chamber. Live females were kept in cups and were fed a sugar solution (5%) for 72 h and delayed mortality was followed. The results were reported as passing, blood fed and mortality rates.ResultsMosquito populations that are resistant to the insecticides in the bed net, exhibited high mortality (60%) in the presence of the BSPs. Untreated bed nets with patches in the tunnel test killed 66–95% of the mosquitoes that landed and untreated bed nets were superior to treated bed nets.ConclusionBSPs efficiently kill resistant mosquitoes that land on treated and untreated bed nets and thus could ultimately reduce the number of vector-borne malarial mosquitoes.

Arylcyclopropylcarbinyl alcohols (1) and (2) rearrange to the homoallyl alcohols (3) and (4) when irradiated in cyclohexane and form homoallyl ethers (8) and (9) in addition to (3) and (4) when irradiated in methanol.

Arylcyclopropylcarbinyl alcohols (1) and (2) rearrange to the homoallyl alcohols (3) and (4) when irradiated in cyclohexane and form homoallyl ethers (8) and (9) in addition to (3) and (4) when irradiated in methanol.