1. Zwitterionic near infrared fluorescent agents for noninvasive real-time transcutaneous assessment of kidney function
- Author
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Rodeghiero Federica, Jiaguo Huang, Norbert Gretz, Stefanie Weinfurter, Johannes Pill, Rossana Perciaccante, Cristina Daniele, and Leopoldo Della Ciana
- Subjects
Kidney ,010405 organic chemistry ,Reabsorption ,Chemistry ,Renal function ,General Chemistry ,Urine ,Glomerulus (kidney) ,010402 general chemistry ,medicine.disease ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,medicine.anatomical_structure ,Biochemistry ,In vivo ,Biophysics ,medicine ,Kidney disease - Abstract
We developed novel zwitterionic near infrared (NIR) fluorescent agents (ABZWCY-HPβCD and AAZWCY-HPβCD), which exhibit favorable hydrophilicity, low plasma protein binding, high stability and non-toxicity. These attractive characteristics ensure that they are excreted rapidly, without any skin accumulation or metabolism in vivo. More importantly, zwitterionic HPβCD based agents can be efficiently filtrated by the glomerulus and completely excreted through the kidneys into urine without reabsorption or secretion in the kidney proximal tubule. Relying on these novel zwitterionic NIR agents and a transcutaneous device, we demonstrate a rapid, robust and biocompatible approach for assessing kidney function in rat models of both healthy rats and those with kidney disease, without the need for time-consuming blood/urine sample preparation. Our work provides a promising tool for in vivo real-time non-invasive kidney function assessment in preclinical applications.
- Published
- 2017
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