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The lack of reliable techniques to accurately quantify bacterial carbonate production and a tractable genetic system limits our ability to thoroughly understand the metabolic basis for bacterial calcification. In this work, we demonstrate bacterially induced CaCO3 precipitation in the model organism, Escherichia coli K-12, and develop new quantitative methods for carbonate mineral production using inductively coupled plasma-optical emission spectroscopy (ICP-OES) and an image analysis approach repurposed for agar cultures. These assays allowed us to assess the impact of various calcium sources and environmental factors on crystalline CaCO3 formation in E. coli. Our data demonstrate that Ca-acetate and Ca-succinate yield the greatest CaCO3 output in E. coli, while a preliminary study demonstrated microclimatic influences on crystal growth and size. This work helps lay the foundation for using E. coli as a model bacterial species to probe the genetic elements that promote calcification in Bacteria, with the potential to identify previously undescribed pathways for precipitation.

Supplemental material, sj-docx-1-qjp-10.1177_17470218221078869 for Cognition and affect in imagined and recalled scenarios as a function of a specificity manipulation: An experience sampling study by Darya L Zabelina, Peyton E Jennings and Jennifer C Veilleux in Quarterly Journal of Experimental Psychology

Supplemental material, sj-docx-1-qjp-10.1177_17470218221078869 for Cognition and affect in imagined and recalled scenarios as a function of a specificity manipulation: An experience sampling study by Darya L Zabelina, Peyton E Jennings and Jennifer C Veilleux in Quarterly Journal of Experimental Psychology

The pyramids of ancient Egypt are said to have shone brilliantly in the sun. Surfaces of polished limestone would not only have reflected diffusely in all directions, but would also likely have produced specular reflections in particular directions. Reflections toward points on the horizon would have been visible from large distances. On a particular day and time when the sun was properly situated, an observer stationed at a distant site would have seen a momentary flash as the sun's reflection moved across the face of the pyramid. The positions of the sun that are reflected to the horizon are confined to narrow arcs in the sky, one arc for each side of the pyramid. We model specular reflections from the pyramid of Khufu and derive the annual dates and times when they would have been visible at important ancient sites. Certain of these events might have coincided with significant dates on the Egyptian calendar, as well as with solar equinoxes, solstices and cross-quarter days. The celebration of Wepet-Renpet, which at the time of the pyramid's construction occurred near the spring cross-quarter day, would have been marked by a specular sweep of sites on the southern horizon. On the autumn and winter cross-quarter days reflections would have been directed to Heliopolis. We suggest that on those days the pyramidion of Khafre might have been visible in specular reflection over the truncated top of Khufu's pyramid., Comment: Minor changes and corrections (26Dec2022)

Additional file 1: Table S1. Schedule of events for both studies. Table S2. Growth parameters of the 3D7-MBE-008 pilot bank (n = 2) compared to 3D7 bank using historical data (n = 177). Table S3. Growth parameters of the 3D7-V1 pilot bank (n = 2) compared to 3D7-V2 bank using historical data (n = 177). Table S4. Overall clinical score recorded for each participant during 3D7-MBE-008 study. Table S5. Overall clinical score recorded for each participant during 3D7-V1 study.

© The Author(s) 2020., How the genome activates or silences transcriptional programmes governs organ formation. Little is known in human embryos undermining our ability to benchmark the fidelity of stem cell differentiation or cell programming, or interpret the pathogenicity of noncoding variation. Here, we study histone modifications across thirteen tissues during human organogenesis. We integrate the data with transcription to build an overview of how the human genome differentially regulates alternative organ fates including by repression. Promoters from nearly 20,000 genes partition into discrete states. Key developmental gene sets are actively repressed outside of the appropriate organ without obvious bivalency. Candidate enhancers, functional in zebrafish, allow imputation of tissue-specific and shared patterns of transcription factor binding. Overlaying more than 700 noncoding mutations from patients with developmental disorders allows correlation to unanticipated target genes. Taken together, the data provide a comprehensive genomic framework for investigating normal and abnormal human development., The work was supported by Wellcome grants 088566, 097820 and 105610, with additional support from MRC project grants MR/L009986/1 to N.B. and N.A.H., MR/ J003352/1 to K.P.H., and MR/000638/1 and MR/S036121/1 to N.A.H. R.E.J. was an MRC clinical research training fellow, and S.J.W. was an MRC doctoral account PhD student. J. L.G.S. was supported by the Marató TV3 Fundacion (Grant No. 201611).

Supplemental material, DS_10.1177_0001839219835867 for Gender Gaps in Perceived Start-up Ease: Implications of Sex-based Labor Market Segregation for Entrepreneurship across 22 European Countries by Vartuhi Tonoyan, Robert Strohmeyer and Jennifer E. Jennings in Administrative Science Quarterly

Supplemental material, Wangner_et_al_supplementary_information for A 2000-year record of ocean influence on Jakobshavn Isbræ calving activity, based on marine sediment cores by David J Wangner, Anne E Jennings, Flor Vermassen, Laurence M Dyke, Kelly A Hogan, Sabine Schmidt, Kurt H Kjær, Mads F Knudsen and Camilla S Andresen in The Holocene

Summary: To interrogate the alternative fates of pancreas and liver in the earliest stages of human organogenesis, we developed laser capture, RNA amplification, and computational analysis of deep sequencing. Pancreas-enriched gene expression was less conserved between human and mouse than for liver. The dorsal pancreatic bud was enriched for components of Notch, Wnt, BMP, and FGF signaling, almost all genes known to cause pancreatic agenesis or hypoplasia, and over 30 unexplored transcription factors. SOX9 and RORA were imputed as key regulators in pancreas compared with EP300, HNF4A, and FOXA family members in liver. Analyses implied that current in vitro human stem cell differentiation follows a dorsal rather than a ventral pancreatic program and pointed to additional factors for hepatic differentiation. In summary, we provide the transcriptional codes regulating the start of human liver and pancreas development to facilitate stem cell research and clinical interpretation without inter-species extrapolation. : Jennings et al. present the first transcriptomes at the inception of liver and pancreas development in very early post-implantation human embryos. By computational analysis, they impute regulatory signatures in each organ, including new transcription factors in pancreas and new guidance for human pluripotent stem cell differentiation. Keywords: human, pancreas, liver, development, embryo, transcriptome, RNA sequencing, stem cell

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To compare north and south polar marine paleoenvironments over the last 30,000 years, comparable chronological (radiocarbon) records must be developed and refined. Many areas in the polar regions do not preserve marine carbonates (foraminifera, mollusks), and thus age determinations, of necessity, are based on the acid-insoluble organic (AIO) fraction of the sediment. Although AIO ages are problematic and rarely used in the Arctic, they provide reasonable and consistent chronologies for the Ross Sea, Antarctica. AIO dates are meaningful in the Ross Sea because there are relatively high levels of productivity, good preservation of marine biogenic material in the sediment, and little input of terrigenous sediment and old/dead carbon. Event stratigraphy based upon proxy records of biogenic silica and ?13C can be used to assess the reliability of the AIO dates and surface age corrections. Reconstructed time-series of changes in the biogenic silica content of cores from the western Ross Sea show apparent similarities with the ‘classic’deglacial climate sequence of the northern North Atlantic. Once the absolute ages of the antarctic AIO dates are constrained by independently dated records to validate surface age corrections, it will be possible to directly compare the timing of events such as ice-rafting events in the sedimentary record.

Subunit D of multisubunit RNA polymerase from many species of archaea is predicted to bind one to two iron-sulfur (Fe-S) clusters, the function of which is unknown. A survey of encoded subunit D in the genomes of sequenced archaea revealed six distinct groups based on the number of complete or partial [4Fe-4S] cluster motifs within domain 3. Only subunit D from strictly anaerobic archaea, including all members of the Methanosarcinales, are predicted to bind two [4Fe-4S] clusters. We report herein the purification and characterization of Methanosarcina acetivorans subunit D in complex with subunit L. Expression of subunit D and subunit L in Escherichia coli resulted in the purification of a D-L heterodimer with only partial [4Fe-4S] cluster content. Reconstitution in vitro with iron and sulfide revealed that the M. acetivorans D-L heterodimer is capable of binding two redox-active [4Fe-4S] clusters. M. acetivorans subunit D deleted of domain 3 (D Delta D3) was still capable of co-purifying with subunit L but was devoid of [4Fe-4S] clusters. Affinity purification of subunit D or subunit D Delta D3 from M. acetivorans resulted in the co-purification of endogenous subunit L with each tagged subunit D. Overall, these results suggest that domain 3 of subunit D is required for [4Fe-4S] cluster binding, but the [4Fe-4S] clusters and domain 3 are not required for the formation of the D-L heterodimer. However, exposure of two [4Fe-4S] cluster-containing D-L heterodimer to oxygen resulted in loss of the [4Fe-4S] clusters and subsequent protein aggregation, indicating that the [4Fe-4S] clusters influence the stability of the D-L heterodimer and therefore have the potential to regulate the assembly and/or activity of RNA polymerase in an oxidant-dependent manner.

Personal narratives have been shown to play an important role in creating a stable sense of self, yet little research has examined this in experimental designs. Thus, this study explored the utility of narrative, in comparison to other mechanisms (e.g., self-affirmation, distraction), for coping with threats to self-concept by examining affective and cognitive repair after experiencing a threat. Participants (N = 331) received false physiological feedback suggesting a prejudiced response to African Americans and obese people and were induced to complete one of five repair techniques. Participants also completed affect and self-concept measures pre-study, post-threat, and post-repair. Overall, threat-specific and high-point narratives did not differ from other groups in their ability to overcome selfconcept threat, but high-point narratives were particularly effective in enacting affective repair. However, individual differences in the threat-specific narratives moderated the effectiveness of this condition, so that more skilled story-telling was associated with more repair. These results suggest that while narrative processing is an effective method of maintaining a stable self-concept for people who have the appropriate skills and content to draw on, it is by no means necessary. Other self-maintenance techniques, such as remembering positive experiences unrelated to the threat, are also effective in maintaining self-meaning and may be more strategic responses to situations when the capacity for quality narrative reflection is low.

A modified Kaluza-Klein theory is proposed in which propagation takes place only at the speed of light. The propagation can be confined to a small volume, forming a particle with rest mass. The usual four space-time coordinates locate the confinement volume, and Kaluza's fifth coordinate is replaced by an internal degree of freedom. Electromagnetism corresponds to a gauge field on the phase of the internal motion. Self-gravity might create the confinement, as in a geon, but the particle would have a Planck mass. This large mass could be made closer to the masses of observed particles if gravity were allowed to increase in strength within the confinement volume., Comment: 6 pages, 10 equations

Absorption lines of hot water have been identified in the infrared spectra of Betelgeuse (α Orionis) and Antares (α Scorpii) near 12.3 micrometers (811 to 819 wavenumbers). The water lines originate in the atmospheres of the stars, not in their circumstellar material. The spectra are similar in structure to umbral sunspot spectra. Pure rotation water lines of this type will occur throughout the spectra of cool stars at wavelengths greater than 10 micrometers. From the water spectra, the upper limit for the temperature in the line formation region in both stars is 2800 kelvin. The water column density in both stars is (3 ± 2) × 1018 molecules per square centimeter, yielding an abundance relative to atomic hydrogen ofn(H2O)/n(H) ≈ 10− 7.

Monthly water samples were taken and analyzed to determine the water quality of the Salt River in Anderson and Spencer counties Kentucky prior to the river's impoundment. Sediments from the area watershed were analyzed to total acid digestion, barium chloride extraction, and aqueous extraction methods. Rainwater and runoff water were collected and analyzed for major cations and anions from two sites in the watershed. The Salt River at Taylorsville is characterized by hard water with high levels of calcium (33.5-74.8 mg/1), bicarbonate (136-236 mg/l), specific conductance (200-535 μmhos/cm), and sulfate (16.5-71.5 mg/l). Nitrates (0.6-5.7 mg/l), phosphates (0.2-2.4 mg/l), sodium (3.2-20.3 mg/l), and potassium (1.3-5.6 mg/l), are moderate. Iron, manganese, copper, and nitrites are less than 0.5 mg/1. Suspended solids in the river (4.0-l ,684.0 mg/l) are highly variable and directly related to fluctuations in discharge. Sediments from the Salt River Basin are high in potassium (12.4-213.3 mg/g) and iron (23.4-135.1 mg/g), with moderate levels of calcium (0.8-45.7 mg/g), sodium (4.5-10.5 mg/g), magnesium (3.2-6.3 mg/g), and phosphate (1.3-15.3 mg/g). Approximately 10% of the total ionic composition of these sediments is exchangeable and may be extracted with barium chloride. Calcium (309-3,292 μg/g), was the most readily adsorbed cation, with lower levels of potassium (17.6-490.5 μg/g), sodium (12.9-458.1 μg/g), and magnesium (89.4-266.2 μg/g). In the aqueous extractions, calcium (18-486 μg/g), potassium (16.6-69.5 μg/g), sodium (11.1-30.8 μg/g), and magnesium (6.6-68.7 μg/g) comprised about 10% of the exchangeable fraction. Ranges of rainwater ions from the Salt River Basin were: sulfate (8.3-27.8 mg/l), calcium (0.3-10.7 mg/l), potassium (0.4-15.4 mg/l), sodium (0.0-0.7 mg/l), and magnesium (0.1-2.8 mg/l). Ionic composition and sediment yield of runoff water was variable and was related to magnitude of rainfall and runoff sampler placement. Ranges for selected constituents at the two samplers near Taylorsville were: suspended solids (44.0-8,808.0 mg/l), potassium (1.1-84.0 mg/l), magnesium (l.5-7.1 mg/l), calcium (9.5-33.0 mg/l), and sodium (0.6-3.0 mg/l). Calcium and bicarbonate in the Salt River originate from weathering of calcite, although mole ratios of these two ions greater than 1:2 suggest that weathering of magnesium carbonates also contributes bicarbonate to the water. Carbonate equilibrium calculations using field pH and ionic strength suggest calcium is at saturation in the Salt River. High levels of sulfate in rainwater indicate some of this anion may be introduced into the area watershed by atmospheric precipitation. Funding information: The work on which this report is based was supported in part by funds provided by the Office of Water Research and Technology, United States Department of the Interior, as authorized under the Water Resources Research Act of 1964.

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Samples of water, bottom fauna, and fishes were collected from 66 stations in the Salt River and one of its principal tributaries, the Beech Fork and its tributary, the Chaplin River, Kentucky. Precipitation ranged from 38.86 inches (1969) to 58.04 inches (1970), an increase of nearly 50 percent with marked fluctuations in discharge. Intensive comparisons of phosphates, sulfates, specific conductance, total alkalinity, total hardness, and turbidity showed the streams to be relatively clean and healthy. Nearly 300 different kinds of benthic organisms and other macroinvertebrates have been collected and identified from the basin. Detailed studies of caddisflies and stream drift are under way along with the development of computer programs for diversity indices of the various organisms. Twenty-eight species of bivalve mussels and representatives of six genera of snails have been collected including the Asiatic clam Corbicula mülleri. Among the vertebrates, 60 species of fishes have been collected and identified along with 22 amphibians and 21 reptiles. Nearly 150 species of birds have been identified in the area. An economic study of Spencer County revealed that there has been a decrease in the human population along with a general decline in the overall economic picture of the county as indicated by a retarded rate of growth in annual per capita income and a decline in total retail sales within the county over the past decade. The highway system in the county consists of largely Class 4, 5, and 6 roads which, because of the topography, are generally narrow, crooked, and hilly. However, Spencer County is almost completely encircled by interstate highways within ten miles of its borders. Funding information: The work on which this report is based was supported in part by funds provided by the Office of Water Resources Research, United States Department of the Interior, as authorized under the Water Resources Research Act of 1964. This study could not have been undertaken without the cooperation and financial assistance of the U.S. Department of the Interior, Office of Water Resources Research, afforded through the Water Resources Institute of the University of Kentucky.

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