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The entire Peninsular Malaysia is situated on Sunda Tectonic Plate, which is subjected to motion at a prominent rate. All the geodetic infrastructures, including geodetic markers, benchmarks, Continuous Operating Reference Stations and Cadastral Reference Marks have moved away from their original position, and their existing coordinates are no longer reliable and secure to use. There are 10 geodetic markers around UTM that are subjected to the issue above. This study aims to redefine new geodetic and plane coordinates on UTM geodetic markers. Two units of Trimble NetR9 Geodetic Type Receiver are used to execute 3D GNSS Control Network on geodetic markers as well as Standard Benchmarks. Receiver Independent Exchange data of Continuous Operating Reference Stations and gravimetric geoid of MyGeoid model are retrieved from Department of Survey and Mapping Malaysia. Trimble Business Center, Golden Surfer 8 and StarNet are used as post-processing, geoid interpolation and one-dimensional network adjustment software, respectively. New sets of geodetic and plane coordinates along with orthometric heights are produced for these 10 geodetic markers. Eventually, UTM geodetic markers are tied to Geocentric Datum Malaysia 2000 (2016) and National Geodetic Vertical Datum, providing reliable horizontal and vertical reference for land surveying work to fulfil both industrial and educational purposes.Keywords: Coordinate redefinition, geodetic coordinate, plane coordinate, geodetic marker

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The centrosymmetric title NiII complex, [Ni(C9H10NO2)2(C10H14N2O)2(H2O)2], contains two dimethyl­amino­benzoate (DMAB), two diethyl­nicotinamide (DENA) ligands and two water mol­ecules, all of them monodentate. The four O atoms in the equatorial plane around the NiII atom form a slightly distorted square-planar arrangement, while the slightly distorted octa­hedral coordination is completed by the two pyridine N atoms of the DENA ligands in axial positions. The NiII atom is displaced by 0.681 (1) A out of the least-squares plane of the carboxyl­ate group. The dihedral angle between the carboxyl­ate group and the adjacent benzene ring is 5.61 (7)°, while the pyridine and benzene rings are oriented at a dihedral angle of 73.20 (4)°. An intra­molecular O—H⋯O hydrogen bond results in the formation of a six-membered ring with a twisted conformation. In the crystal structure, inter­molecular O—H⋯O and C—H⋯O hydrogen bonds link mol­ecules into a three-dimensional network. Two weak C—H⋯π inter­actions are also present.

Aptamer selection against novel infections is a complicated and time-consuming approach. Synergy can be achieved by using computational methods together with experimental procedures. This study aims to develop a reliable methodology for a rational aptamer in silico et vitro design. The new approach combines multiple steps: (1) Molecular design, based on screening in a DNA aptamer library and directed mutagenesis to fit the protein tertiary structure; (2) 3D molecular modeling of the target; (3) Molecular docking of an aptamer with the protein; (4) Molecular dynamics (MD) simulations of the complexes; (5) Quantum-mechanical (QM) evaluation of the interactions between aptamer and target with further analysis; (6) Experimental verification at each cycle for structure and binding affinity by using small-angle X-ray scattering, cytometry, and fluorescence polarization. By using a new iterative design procedure, structure- and interaction-based drug design (SIBDD), a highly specific aptamer to the receptor-binding domain of the SARS-CoV-2 spike protein, was developed and validated. The SIBDD approach enhances speed of the high-affinity aptamers development from scratch, using a target protein structure. The method could be used to improve existing aptamers for stronger binding. This approach brings to an advanced level the development of novel affinity probes, functional nucleic acids. It offers a blueprint for the straightforward design of targeting molecules for new pathogen agents and emerging variants.