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Groundwater quality of Jalalabad cantonment area in Sylhet city is deteriorating. The study was conducted for assessing the ground water quality in drinking purpose of Jalalabad cantonment area in Sylhet city. For human survival, water is a vital resource. Total twenty-five water samples were collected from different parts of Jalalabad Cantonment area from deep aquifer-based tube wells. The collected water samples were analyzed for different physicochemical properties like Color, taste, odor, pH, electrical conductivity (EC), total dissolved solids (TDS), calcium, magnesium, sodium, potassium, boron, sulphur, copper, iron, manganese, and zinc concentrations. All the analyzed parameters compared with the standards prescribed by World Health Organization (WHO) and Bureau of Bangladesh Standard (BBS), in order to assess the ground water quality for drinking purpose. For different parameters of drinking water, the correlation matrix was also calculated. Almost all the physicochemical parameters were found within the prescribed permissible limit. Findings show that some of the parameters Cu and Zn were negatively associated with TDS, Ca2+, Mg2+, S contents.

IntroductionSalinity is a complex environmental stress that affects the growth and production of rice worldwide. But there are some rice landraces in coastal regions that can survive in presence of highly saline conditions. An understanding of the molecular attributes contributing to the salinity tolerance of these genotypes is important for developing salt-tolerant high yielding modern genotypes to ensure food security. Therefore, we investigated the role and functional differences of two K+ salt-responsive transporters. These are OsTPKa or Vacuolar two-pore potassium channel and OsHAK_like or a hypothetical protein of the HAK family. These transporters were selected from previously identified QTLs from the tolerant rice landrace genotype (Horkuch) and sensitive genotype (IR29).MethodsIn silico comparative sequence analysis of the promoter sequences of two these genes between Horkuch and IR29 was done. Real-Time expression of the selected genes in leaves and roots of IR29 (salt-sensitive), I-14 and I-71 (Recombinant Inbred Lines of IR29(♀)× Horkuch), Horkuch and Pokkali (salt-tolerant) under salt-stress at different time points was analyzed. For further insight, OsTPKa and OsHAK_like were chosen for loss-of-function genomic analysis in Horkuch using the CRISPR/Cas9 tool. Furthermore, OsTPKa was chosen for cloning into a sensitive variety by Gateway technology to observe the effect of gain-of-function.ResultsThe promoter sequences of the OsTPKa and OsHAK_like genes showed some significant differences in promoter sequences which may give a survival advantage to Horkuch under salt-stress. These two genes were also found to be overexpressed in tolerant varieties (Horkuch and Pokkali). Moreover, a coordinated expression pattern between these two genes was observed in tolerant Horkuch under salt-stress. Independently transformed plants where the expression of these genes was significantly lowered, performed poorly in physiological tests for salinity tolerance. On the other hand, positively transformed T0 plants with the OsTPKa gene from Horkuch consistently showed growth advantage under both control and salt stress.DiscussionThe poor performance of the transgenic plants with the down-regulated genes OsTPKa and OsHAK_like under salt stress supports the assumption that OsTPKa and OsHAK_like play important roles in defending the rice landrace Horkuch against salt stress, minimizing salt injury, and maintaining plant growth. Moreover, the growth advantage provided by overexpression of the vacuolar OsTPKa K+ transporter, particularly under salt stress reconfirms its important role in providing salt tolerance. The QTL locus from Horkuch containing these two transporters maybe bred into commercial rice to produce high-yielding salt tolerant rice.

ChatGPT is a large language model developed by OpenAI. Despite its impressive performance across various tasks, no prior work has investigated its capability in the biomedical domain yet. To this end, this paper aims to evaluate the performance of ChatGPT on various benchmark biomedical tasks, such as relation extraction, document classification, question answering, and summarization. To the best of our knowledge, this is the first work that conducts an extensive evaluation of ChatGPT in the biomedical domain. Interestingly, we find based on our evaluation that in biomedical datasets that have smaller training sets, zero-shot ChatGPT even outperforms the state-of-the-art fine-tuned generative transformer models, such as BioGPT and BioBART. This suggests that ChatGPT's pre-training on large text corpora makes it quite specialized even in the biomedical domain. Our findings demonstrate that ChatGPT has the potential to be a valuable tool for various tasks in the biomedical domain that lack large annotated data., Comment: Accepted by BioNLP@ACL 2023

Rehabilitation needs are rising globally; however, the rate is strikingly higher in low- and middle-income countries (LMICs). Like many LMICs, the situation of rehabilitation services for persons with disabilities (PwDs) in Bangladesh is mostly unknown. We assessed the current situation of rehabilitation services for PwDs in Bangladesh. This mixed-method study incorporated an online survey of rehabilitation service providers and a scoping review of documents published on rehabilitation services for PwDs in Bangladesh. Descriptive and thematic analyses were completed. A total of 1102 rehabilitation service providers were interviewed, and 36 documents were reviewed. Rehabilitation services for PwDs were found not integrated into the mainstream health services, financing mechanisms, information systems, and health policies in Bangladesh. There are 6.8 rehabilitation units for 1 million people, and 6.2% of them are located in rural areas. In terms of the rehabilitation workforce, there are 9.4 physiotherapists, 1.3 occupational therapists, 0.9 speech and language therapists, and 0.2 prosthetist and orthotists for 1 million people in Bangladesh. Majority (66.3%) of rehabilitation services require an out-of-pocket payment. A critical shortage and uneven distribution of the rehabilitation workforce are evident, indicating a likelihood of very high unmet rehabilitation needs in Bangladesh. To strengthen the rehabilitation capacity of Bangladesh, rehabilitation services should be integrated into mainstream health policies and programs with a special focus on the rehabilitation workforce training, recruitment and distribution, and allocation of resources.Implications for rehabilitationRehabilitation services in Bangladesh are mostly provided by the private sector, although the government of Bangladesh operates a small number of rehabilitation services outside of the mainstream public health service delivery system.Rehabilitation services are lacking in the primary and secondary health facilities, while services at tertiary level public hospitals are mainly provided by medical technologists in the absence of a qualified rehabilitation workforce.A severe shortage of rehabilitation workforce coupled with an uneven distribution of the existing limited number of rehabilitation services and out-of-pocket expenditures might result in inadequate access and poor rehabilitation service uptake amongst persons with disabilities (PwDs) in Bangladesh.Robust governance and leadership are needed to monitor the implementation of existing legislations and policies and develop strategies to improve the situation of rehabilitation services for PwDs in Bangladesh. Rehabilitation services in Bangladesh are mostly provided by the private sector, although the government of Bangladesh operates a small number of rehabilitation services outside of the mainstream public health service delivery system. Rehabilitation services are lacking in the primary and secondary health facilities, while services at tertiary level public hospitals are mainly provided by medical technologists in the absence of a qualified rehabilitation workforce. A severe shortage of rehabilitation workforce coupled with an uneven distribution of the existing limited number of rehabilitation services and out-of-pocket expenditures might result in inadequate access and poor rehabilitation service uptake amongst persons with disabilities (PwDs) in Bangladesh. Robust governance and leadership are needed to monitor the implementation of existing legislations and policies and develop strategies to improve the situation of rehabilitation services for PwDs in Bangladesh.

A paradigm shift from assessment of learning (AoL) towards assessment for learning (AfL) and assessment as learning (AaL) is occurring across many countries. A similar trend is obvious in the Australian secondary education system. Teachers are encouraged to adopt a number of strategies, such as sharing assessment criteria and feedback in order to improve learning and raise educational achievement. However, not enough is known about the extent to which teachers have embraced this change in a core key learning area like Science. Even less is known about the use of assessment criteria. Hence, this study aims to explore teachers’ use of assessment criteria in practical science activities (PSAs) by following an ecological perspective. The qualitative data collected from three case studies (two government schools and one independent school) was analysed using a constant comparative method. To establish the representativeness of the case studies, quantitative data was collected from 310 teachers working in government and independent schools across New South Wales and analysed using descriptive and exploratory factor analysis. The findings suggest that science teachers in NSW develop criteria without student input. They use the criteria for various purposes, but most commonly for marking and reporting student achievement. Explicit criteria are used for summative assessments only. Moreover, teachers generally don’t get students to use criteria for self- and peer-assessment either. Thus, teachers’ use of assessment criteria primarily reflects the philosophy of AoL and does not really align with AfL and AaL principles, despite the importance of such assessment given in the literature, school policies and briefings by department heads and principals in case studies. Explanations for this lack of uptake of assessment for/as learning in practice are not always clear. Teachers’ use of criteria can be influenced by numerous factors: time constraints, lack of teaching experience, collaboration with colleagues and other contextual factors. However, a gap between teachers and school leaders understanding of the role and use of criteria is a problem for policy implementation. Therefore, this study stresses the importance of providing continuous professional development and support to bridge practice and policy in relation to teachers’ use of assessment criteria.

The establishment of a model system of neoplastic transformation of normal human cells has been attempted with a chemical carcinogen, 4-nitroquinoline 1-oxide (4NQO). In the course of these experiments, it was noticed that immortalization of human cells is a multi-step process involving several mutational genetic events. Thus, chromosomal changes which occurred during the process of immortalization of human fibroblasts were examined. To accomplish immortalization, fibroblasts obtained from an embryo were repeatedly treated with 10-6M4NQO from primary culture to passage 51 (59 treatments in total). Before immortalization, some chromosomes (especially, chromosomes 2, 6, 8, 10, 11, 12, 15, 19, and 20), were lost at a relatively high frequency. After immortalization, the chromosomes distributed so broadly in the triploid to hypotetraploid region without a distinct modal number or without marker chromosomes that it was difficult to identify the specific chromosomes related to the immortalization of human cells. No specific structural chromosomal changes were detected. Although the significance of such chromosome changes in relation to immortalization is not clear, the loss of some specific chromosomes suggests that genes which are involved in cellular aging and which suppress immortalization may have been lost in the immortalization process.

Refereed/Peer-reviewed Background: Systemic lupus erythematosus (SLE) is an autoimmune disorder in which the body's defense system wrongly attacks healthy body tissues. The objective of this current setup was to quantify and compare the serum concentration of ascorbic acid (Vit-C), malondialdehyde (MDA), c-reactive protein (CRP) and trace elements (Cu, Fe, Mn and Zn) in SLE and normal subjects. Methods: The proposed case-control study was performed with 25 SLE patients and 25 healthy subjects as case and control, respectively. The serum level of malondialdehyde (MDA) and vitamin C was evaluated by UV spectrophotometric method. For the determination of CRP, the latex agglutination method was used, whereas serum trace elements were estimated by atomic absorption spectroscopy (AAS). Results: This analysis demonstrated that patients with SLE possessed a significant (p < 0.001) higher level of MDA and lower level of vitamin C compared to control subjects. Pearson's correlation analysis found negative correlation between the serum level of MDA and vitamin C (r= -0.023, p = 0.887) for patients while control group also possessed similar result (r= -0.157, p = 0.453). The current findings have also revealed that serum level of Zn and Cu in SLE patients was significantly (p < 0.05) lowered to that of the control group, while serum level of Mn also showed a similar scenario. During Pearson's correlation analysis a significantly (p < 0.05) negative correlation was found between Zn and Mn (r= -0.410, p = 0.042) in patients' group. Conclusion: Although our study was limited to a small sample size and confined to a particular area of the country, the study results support a significant role of antioxidants, CRP, and trace elements in the generation of SLE and, therefore, recommends a large spectrum study of the associations between SLE and these biochemical parameters. usc