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The suppression of the SOS response has been shown to enhance the in vitro activity of quinolones. Furthermore, Dam-dependent base methylation has an impact on susceptibility to other antimicrobials affecting DNA synthesis.

Introduction Currently, there is no consensus on the indication of prophylactic surgery of the nodal compartments in the treatment of medullary thyroid carcinoma (MTC). The aim of our study was to perform a correlation study between preoperative calcitonin (basalCT) values and lymph node involvement to establish a criterion on which to base prophylactic surgery in these patients. Material and Methods We conducted an observational, retrospective and multicentre study with 29 hospitals. Patients over 18 years of age with a diagnosis of MTC with a pre-surgical calcitonin registry were included. The minimum surgery in all patients had to have been total thyroidectomy (TT) with central compartment lymph node dissection (CCLND). Receiver operating characteristic (ROC) curve analysis was used to establish basalCT cut-off values as predictors of postoperative lymph node involvement. Results A total of 244 patients were included. Baseline calcitonin (basalCT) was a good predictor of nodal involvement (AUC 0.718 and 95%CI 0.66–0.978). Heritability was identified as a preoperative factor correlated with baseline tumour CT values (p = 0.000). With a probability of lymph node involvement below 10%, new cut-off points were established. A prophylactic bilateral lateral lymph node dissection in sporadic tumours should be performed at a basalCT > 600 pg/mL; in the case of RET-mutated tumours this value would be 200 pg/mL. Conclusion The baseline CT value is a good predictor of postoperative lymph node involvement in MTC, however, cut-off points should depent on the hereditary nature of the tumour.

Introduction In bioprinting techniques, tissue engineering from 3D models to generate the desired geometry is as important as cell proliferation, as it will be key to the survival of the printed tissue and its functional success. Methods Alginate-based hydrogels were used because they are the most widely used by the scientific community for bioprinting. The alginate-based hydrogels used were characterised using a rheometer to obtain the boundary conditions to be applied in the computational software. A 2D axisymmetric model together with a two-phase flow level set interface of a bioprinting nozzle and a computational model of a human tooth were carried out to verify the results obtained. Results The data showed that droplet detachment occurs at lower pressures based on the higher the percentage of alginate. In addition, samples with a higher percentage of alginate were less sensitive to pressure during extrusion. Conclusions

Introduction In bioprinting, it is essential to correctly characterise the materials to be used in order to ensure the highest cell viability while maintaining the best structural properties of the hydrogels. Methods Alginate-based hydrogels with different concentrations have been characterised because they are currently the ones with the best printing properties according to the literature. The concentrations chosen were 3.5%, 4% and 5% alginate, and then a scaffold was bioprinted with the one with the best mechanical properties. Finally, a 3D model of a tooth was bioprinted to check the integrity of the hydrogel characterised as a somewhat more complex structure than a simple scaffold. Results It could be observed that, despite suffering a similar viscosity loss to the rest, the sample with the best viscosity properties when subjected to shear stresses was the one containing 5% alginate concentration. When a simple scaffold was bioprinted, it was observed that the result was positive, as the visual analysis corresponded to the parameters programmed in 3D design software. Likewise, the subsequent bioprinting of the dental model showed good results. Conclusions The hydrogels characterised by rheometry showed similar behaviour with respect to viscosity. Furthermore, it was found that the hydrogel with higher viscosity allowed for higher quality bioprints.

Negative attitudes and prejudices towards people with a mental disorder are common across cultures and societies. The stigma associated with mental illness leads to a lower quality of life, given the discrimination and social exclusion suffered by people with this type of disorder. In the field of health, research has shown that doctors and nurses also manifest these types of stigmatizing behaviors and attitudes. The present study aims to create and apply an educational escape room for the purpose of training nursing students in mental health, promoting positive attitudes towards people who have a mental disorder. To do so, a pre-post study was conducted with an experimental group and a control group to determine whether the escape room was effective for the modification of stigmatizing behaviors compared to transmissive lecture class, and a third measurement was made at 6 months only to the experimental group to evaluate whether the changes produced by the escape room were maintained in the long term. The results indicate that the students participating in the study obtained better scores in sensitization and these remain better over time. It is concluded that the escape room used is suitable for the training and sensitization of future nursing professionals in the field of mental health, facilitating the learning of knowledge and positive attitudes towards severe mental disorder., FEDER "A way to make Europe" (Una manera de hacer Europa) UAL2020-SEJ-D1912

The aim of this study is to evaluate the incidence of consecutive strabismus after infantile nystagmus surgery and its potential risk factors. A retrospective study including 89 patients was conducted. Patients presented infantile nystagmus (idiopathic or ocular disease–associated nystagmus) without previous or coincidental strabismus. Sex, age at surgery, amblyopia, botulinum toxin (BT) injection before surgery, spherical equivalent, anisometropia, surgery procedure (Anderson’s or retroequatorial recessions of four horizontal recti), and follow-up were analyzed. Kaplan-Meier and univariate Cox regression were performed. The median age at surgery was 5 years. The median follow-up was 36 months. The incidence of consecutive strabismus was 11.2%. There were eight patients with exotropia and two patients with esotropia. Consecutive strabismus was associated with severe bilateral amblyopia (p = 0.036), previous treatment with BT injection (p = 0.025), and large recessions of the four horizontal muscles (p = 0.001). The hazard ratio for patients with severe bilateral amblyopia was 5.4 (95% CI 1.1–25.8), and for patients previously treated with BT was 6.1 (1.3–29.3). The survival rate was 95.4% at 6 months and 88.5% at 3 years. Severe bilateral amblyopia, previous BT treatment, and type of surgery seem to be associated with consecutive strabismus after infantile nystagmus surgery. Most cases appear within the first months after surgery.

Indexado en Scopus A novel methodology was employed to prepare new nanocomposites with photocatalytic properties based on Ce-doped TiO2 nanoparticles arranged over a layered silicate. The catalysts were porous materials formed by exfoliated silicate layers surrounded by anatase nanoparticles. In this way, the anatase was doped by different amounts of Ce, yielding to catalysts with light absorption properties on the visible region. The photocatalytic behavior was tested for different reactions: adsorption and photocatalysis, showing outstanding and promising results for the removal of bacteria by using solar light as an energy source. The influence of the physicochemical properties of the catalyst and the reaction parameters will be studied in detail to manage new catalysts for the disinfection of drinking water. info:eu-repo/semantics/publishedVersion Revisión por pares

As part of the Early Science Large Millimeter Telescope projects, we report the detection of nine double-peaked molecular lines, produced by a rotating molecular torus, in the ultraluminous infrared galaxies (ULIRG) – Compton-thick active galactic nuclei (AGN) galaxy UGC 5101. The double-peaked lines we report correspond to molecular transitions of HCN, HCO+, HNC, N2H+, CS, C18O, 13CO, and two CN lines; plus the detection of C2H that is a blend of six lines. The redshift search receiver spectra covers the 73–113 GHz frequency window. Low- and high-density gas tracers of the torus have different implied rotational velocities, with a rotational velocity of 149 ± 3 km s−1 for the low-density ones (C18O, 13CO) and 174 ± 3 km s−1 for high-density tracers (HCN, HCO+, HNC, N2H+, CS, and CN). In UGC 5101, we find that the ratio of integrated intensities of HCN to 13CO to be unusually large, probably indicating that the gas in the torus is very dense. Both the column densities and abundances are consistent with values found in AGN, starburst, and ULIRG galaxies. The observed abundance ratios cannot discriminate between X-ray and UV-field-dominated regions.

Diffuse intrinsic pontine gliomas (DIPGs) are the most fatal primary brainstem tumors in pediatric patients. The identification of new molecular features, mediating their formation and progression, as non-coding RNAs (ncRNAs), would be of great importance for the development of effective treatments. We analyzed the DIPGs transcriptome with the HTA2.0 array and it was compared with pediatric non-brainstem astrocytoma expression profiles (GSE72269). More than 50% of the differentially expressed transcripts were ncRNAs and based on this, we proposed a DIPGs ncRNA signature. LncRNAs XIST and XIST-210, and the HBII-52 and HBII-85 snoRNA clusters were markedly downregulated in DIPGs. qPCR assays demonstrated XIST downregulation in all non-brainstem astrocytomas, in a gender, age, and brain location-independent manner, as well as in DIPGs affecting boys; however, DIPGs affecting girls showed both downregulation and upregulation of XIST. Girls’ with longer survival positively correlated with XIST expression. The involvement of ncRNAs in DIPGs is imminent and their expression profile is useful to differentiate them from non-neoplastic tissues and non-brain stem astrocytomas, which suggests their potential use as DIPG biomarkers. In fact, XIST and XIST-210 are potential DIPG prognostic biomarkers.

We use a sample of 706 galaxies, selected as [O ii]λ3727 ([O ii]) emitters in the Survey for High-z Absorption Red and Dead Sources (SHARDS) on the CANDELS/GOODS-N field, to study the differential attenuation of the nebular emission with respect to the stellar continuum. The sample includes only galaxies with a counterpart in the infrared and log10(M∗/M⊙) > 9, over the redshift interval 0.3 ≲ z ≲ 1.5. Our methodology consists in the comparison of the star formation rates inferred from [O ii] and Hα emission lines with a robust quantification of the total star-forming activity (SFR TOT) that is independently estimated based on both infrared and ultraviolet (UV) luminosities. We obtain $f\, =\, E(B-V)_{\mathrm{stellar}}$/E(B - V)nebular = 0.69$^{0.71}_{0.69}$ and 0.55$^{0.56}_{0.53}$ for [O ii] and Hα, respectively. Our resulting f-factors display a significant positive correlation with the UV attenuation and shallower or not-significant trends with the stellar mass, the SFRTOT, the distance to the main sequence, and the redshift. Finally, our results favour an average nebular attenuation curve similar in shape to the typical dust curve of local starbursts., LR-M thanks Casiana Muñoz Tuñón for useful suggestions on this manuscript. LR-M, GR, and AF acknowledge the support from grant PRIN MIUR 2017-20173ML3WW_001. LR-M also acknowledges funding support from the Università degli Studi di Padova - Dipartimento di Fisica e Astronomia ‘G. Galilei’. PGP-G and LC acknowledge support from Spanish Ministerio de Ciencia, Innovación y Universidades through grant PGC2018-093499-B-I00. LC acknowledges also financial support from Comunidad de Madrid under Atracción de Talento grant 2018-T2/TIC-11612. AVG acknowledges support from the European Research Council through the Advanced Grant MIST (FP7/2017-2020, No. 742719). AP gratefully acknowledges financial support from STFC through grants ST/T000244/1 and ST/P000541/1. Analyses were performed in R 3.6.1 (R Core Team 2019).

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited., Aimed at the detection of cosmological gas being accreted onto galaxies in the local universe, we examined the Hα emission in the halo of 164 galaxies in the field of view of the Multi-Unit Spectroscopic Explorer Wide survey (MUSE-Wide) with observable Hα (redshift 50 galaxy radii, which roughly corresponds to the virial radius of the central galaxy. We explore several physical scenarios to explain this Hα emission, among which accretion disks around rogue intermediate-mass black holes fit the observations best. © 2022. The Author(s). Published by the American Astronomical Society., We acknowledge the support from the Spanish Ministry of Science and Innovation, project PID2019-107408GB-C43 (ESTALLIDOS), and from Gobierno de Canarias through EU FEDER funding, project PID2020010050. This research made use of Montage, which is funded by the National Science Foundation under grant No. ACI-1440620, and was previously funded by the National Aeronautics and Space Administration's Earth Science Technology Office, Computation Technologies Project, under Cooperative Agreement Number NCC5-626 between NASA and the California Institute of Technology., With funding from the Spanish government through the Severo Ochoa Centre of Excellence accreditation SEV-2017-0709.

Objective The objective of this study was to investigate whether glycated hemoglobin (HbA1c) is a valid surrogate for evaluating the effectiveness of antihyperglycemic drugs in diabetes mellitus (DM) trials. Methods We conducted a systematic review of placebo-controlled randomized clinical trials (RCTs) evaluating the effect of a treatment on HbA1c (mean difference between groups) and clinical outcomes (relative risk of mortality, myocardial infarction, stroke, heart failure, and/or kidney injury) in patients with DM. Then, we investigated the association between treatment effects on HbA1c and clinical outcomes using regression analysis at the trial level. Lastly, we interpreted the correlation coefficients (R) using the cut-off points suggested by the Institute for Quality and Efficiency in Healthcare (IQWiG). HbA1c was considered a valid surrogate if it demonstrated a strong association: lower limit of the 95 percent confidence interval (95 percent CI) of R greater than or equal to .85. Results Nineteen RCTs were identified. All studies included adults with type 2 DM. None of the associations evaluated was strong enough to validate HbA1c as a surrogate for any clinical outcome: mortality (R = .34; 95 percent CI −.14 to .69), myocardial infarction (R = .20; −.30 to .61), heart failure (R = .08; −.40 to .53), kidney injury (R = −.04; −.52 to .47), and stroke (R = .81; .54 to .93). Conclusions The evidence from multiple placebo-controlled RCTs does not support the use of HbA1c as a surrogate to measure the effectiveness of antihyperglycemic drugs in DM studies.

Purpose Familial papillary thyroid microcarcinoma (FPTMC) can present a more aggressive behavior than the sporadic microcarcinoma. However, few studies have analyzed this situation. The objective is to analyze the recurrence rate of FPTMC and the prognostic factors which determine that recurrence in Spain. Methods Spanish multicenter longitudinal analytical observational study was conducted. Patients with FPTMC received treatment with curative intent and presented cure criteria 6 months after treatment. Recurrence rate and disease-free survival (DFS) were analyzed. Two groups were analyzed: group A (no tumor recurrence) vs. group B (tumor recurrence). Results Ninety-four patients were analyzed. During a mean follow-up of 73.3 ± 59.3 months, 13 recurrences of FPTMC (13.83%) were detected and mean DFS was 207.9 ± 11.5 months. There were multifocality in 56%, bilateral thyroid involvement in 30%, and vascular invasion in 7.5%; that is to say, they are tumors with histological factors of poor prognosis in a high percentage of cases. The main risk factors for recurrence obtained in the multivariate analysis were the tumor size (OR: 2.574, 95% CI 1.210–5.473; p = 0.014) and the assessment of the risk of recurrence of the American Thyroid Association (ATA), both intermediate risk versus low risk (OR: 125, 95% CI 10.638–1000; p p Conclusion FPTMC has a recurrence rate higher than sporadic cases. Poor prognosis is mainly associated with the tumor size and the risk of recurrence of the ATA. Graphical abstract

The protective behaviour of ZrO2-3%molY2O3 sol–gel coatings, deposited with an immersion coating technique on 9Cr-1Mo P91 steel, was evaluated with corrosion monitoring sensors using the electrochemical impedance spectroscopy technique. The tests were carried out in contact with solar salt at 500 °C for a maximum of 2000 h. The results showed the highly protective behaviour of the coating, with the corrosion process in the coated system being controlled by the diffusion of charged particles through the protective layer. The coating acts by limiting the transport of ions and slowing down the corrosive process. The system allowed a reduction in the corrosion rate of uncoated P91 steel. The estimated corrosion rate of 22.62 μm·year−1 is lower than that accepted for in-service operations. The proposed ZrO2-3%molY2O3 sol–gel coatings are an option to mitigate the corrosion processes caused by the molten salts in concentrated solar power plants.

Compositional data play an important role in many disciplines, when the interest is in studying not the total amount but the relative importance or frequency of the involved variables. Due to these proportion/sum constraints, the data belong to a restricted space, the simplex. A special algebraic structure is needed to deal with these kind of data. The use of compositional models has followed an increasing trend during the last years. However, to date not very much has been done about the problem of finding optimal designs for models involving this kind of variables. In this first approach, the application of optimal design theory to models with compositional response is studied, dealing with a possible non-trivial covariance structure between observations. Some analytical results have been obtained, and a clarifying example of application is provided.

The Michaelis-Menten (MM) model is extensively used in biochemistry to describe reaction kinetics. However, deviations from MM kinetics can occur, usually due to an inhibition effect on the amount of substrate. Two different models, describing the kinetic reaction when this type of inhibition occurs, are introduced. Optimal experimental designs methods are used in order to find the best estimation of the parameters in these models. An application to the semicarbazide-sensitive amine oxidase (SSAO) activity towards benzylamine is presented.

In many scientific disciplines, experiments are conducted in order to get information about the model or about different characteristics of the data. Quite often more than one variable is measured, giving rise to multiresponse models. For these models it is usual to assume correlation between different-type observations taken on the same point, and always assume independence between observations taken at different points, even when measuring the same variable. This last independence will be discussed throughout this work, showing that a covariance structure should be considered in some cases, specially when observing multiple characteristics of the same experimental unit (e.g. a chemical reaction) at different instants of time. Analytical results are obtained for the general case and for the bivariate model with some covariance structures. A case study where these ideas are applied will be presented, and the need to include the double covariance structure in the model will be addressed and justified in the final discussion.

Admix of Cu2ZnSnS4 (CZTS) and ZnS phases in thin films were prepared on glass substrate by thermal evaporation from CuS, ZnS and SnS as precursors. Their composition, microstructure and optical properties of thin films were determine using energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), scanning electron microscopy (SEM) and UV–Vis absorption. The conglomerate grain shape of the obtained materialare elongated with dense morphology. Kesterite structure was obtained after heat treatment at 400 °C of precursors. By Rietveld refinement method was determined the presence of CZTS phase and ZnS phase at 65% and at 35%, respectively. By elemental analysis, it was notice Cu loss in the heating process. Band gap value of the resulting material was 1.49 eV. Regarding to the optoelectronic properties for make solar cells, this film shows good photoconductivity.

Preterm infants are generally fed through nasogastric enteral feeding tubes (NEFTs). The aim of this work was to evaluate the role of NEFTs in the initial colonization of the preterm gut and its evolution within the first 2 weeks after birth.For this purpose, fecal and NEFT-derived samples from 30 preterm infants hospitalized in a neonatal intensive care unit (NICU) were collected from birth to the second week of life. Samples were cultivated in ten culture media, including three for the isolation of antibiotic-resistant microorganisms.Isolates (561) were identified by 16S ribosomal RNA gene sequencing. Although the first NEFTs inserted into the neonates after birth were rarely colonized, analysis of NEFTs and fecal samples over time revealed a significant increase in bacterial abundance, diversity, and detection frequency. Results showed a parallel colonization between time-matched NEFTs and fecal samples, suggesting an ongoing bidirectional transfer of bacteria from the neonatal gut to the NEFTs and vice versa.In short-term hospitalization, length is by far the determinant factor for the early colonization of preterm infants. As NEFT populations reflect the bacterial populations that are colonizing the preterm in a precise moment, their knowledge could be useful to prevent the dissemination of antibiotic-resistant strains.The hospital environment modulates preterm colonization immediately after birth. The colonization of preterm feces and NEFTs occurs in parallel. There is an ongoing bidirectional transfer of microorganisms from the neonatal gut to the NEFTs and vice versa. Bacterial communities inside NEFTs could act as reservoirs of antibiotic resistance genes. NEFT populations reflect the bacteria that are colonizing the preterm at a precise moment.

We evaluated human-like the efficacy of intravenous doses of fosfomycin of 8 g every 8 h (8 g/Q8h) and of amikacin (15 mg/kg/Q24h) in monotherapy and in combination against six fosfomycin-heteroresistant Escherichia coli isolates using a hollow-fiber infection model (HFIM). Six fosfomycin-heteroresistant E. coli isolates (four with strong mutator phenotype) and the control strain E. coli ATCC 25922 were used. Mutant frequencies for rifampin (100 mg/liter), fosfomycin (50 and 200 mg/liter), and amikacin (32 mg/liter) were determined. Fosfomycin and amikacin MICs were assessed by agar dilution (AD), gradient strip assay (GSA), and broth microdilution (BMD). Fosfomycin and amikacin synergies were studied by checkerboard and time-kill assays at different concentrations. The efficacies of fosfomycin (8 g/Q8h) and amikacin (15 mg/kg/Q24h) alone and in combination were assessed using an HFIM. Five isolates were determined to be resistant to fosfomycin by AD and BMD, but all were determined to be susceptible by GSA. All isolates were determined to be susceptible to amikacin. Antibiotic combinations were synergistic in two isolates, and no antagonism was detected. In time-kill assays, all isolates survived under fosfomycin at 64 mg/liter, although at 307 mg/liter only the normomutators and two hypermutators survived. Four isolates survived under 16 mg/liter amikacin, and none survived at 45 mg/liter. No growth was detected under combination conditions. In HFIM, fosfomycin and amikacin monotherapies failed to sterilize bacterial cultures; however, the combination of fosfomycin and amikacin yielded a rapid eradication. There may be a risk of treatment failure of fosfomycin-heteroresistant E. coli isolates using either amikacin or fosfomycin in monotherapy. These results support that the amikacin-fosfomycin combination can rapidly decrease bacterial burden and prevent the emergence of resistant subpopulations against fosfomycin-heteroresistant strains.

Ocular optics is normally estimated based on 2,600 measurement points within the pupil of the eye, which implies a lateral resolution of approximately 175 microns for a 9 mm pupil diameter. This is because information below this resolution is not thought to be relevant or even possible to obtain with current measurement systems. In this work, we characterize the in vivo ocular optics of the human eye with a lateral resolution of 8.6 microns, which implies roughly 1 million measurement points for a pupil diameter of 9 mm. The results suggest that the normal human eye presents a series of hitherto unknown optical patterns with amplitudes between 200 and 300 nm and is made up of a series of in-phase peaks and valleys. If the results are analysed at only high lateral frequencies, the human eye is also found to contain a whole range of new information. This discovery could have a great impact on the way we understand some fundamental mechanisms of human vision and could be of outstanding utility in certain fields of ophthalmology.

The aim of this study is to evaluate the incidence of consecutive strabismus after infantile nystagmus surgery and its potential risk factors.A retrospective study including 89 patients was conducted. Patients presented infantile nystagmus (idiopathic or ocular disease-associated nystagmus) without previous or coincidental strabismus. Sex, age at surgery, amblyopia, botulinum toxin (BT) injection before surgery, spherical equivalent, anisometropia, surgery procedure (Anderson's or retroequatorial recessions of four horizontal recti), and follow-up were analyzed. Kaplan-Meier and univariate Cox regression were performed.The median age at surgery was 5 years. The median follow-up was 36 months. The incidence of consecutive strabismus was 11.2%. There were eight patients with exotropia and two patients with esotropia. Consecutive strabismus was associated with severe bilateral amblyopia (p = 0.036), previous treatment with BT injection (p = 0.025), and large recessions of the four horizontal muscles (p = 0.001). The hazard ratio for patients with severe bilateral amblyopia was 5.4 (95% CI 1.1-25.8), and for patients previously treated with BT was 6.1 (1.3-29.3). The survival rate was 95.4% at 6 months and 88.5% at 3 years.Severe bilateral amblyopia, previous BT treatment, and type of surgery seem to be associated with consecutive strabismus after infantile nystagmus surgery. Most cases appear within the first months after surgery.

As insulin sensitivity may help to explain divergences in growth and body composition between native and modern breeds, metabolic responses to glucose infusion were measured using an intra-arterial glucose tolerance test (IAGTT). Iberian (n=4) and Landrace (n=5) barrows (47.0 ± 1.2 kg body weight (BW)), fitted with a permanent carotid artery catheter were injected with glucose (500 mg/kg BW) and blood samples collected at -10, 0, 5, 10, 15, 20, 25, 30, 45, 60, 90, 120 and 180 min following glucose infusion. Plasma samples were analysed for insulin, glucose, lactate, triglycerides, cholesterol, creatinine, albumin and urea. Insulin sensitivity indices were calculated and analysed. Mean plasma glucose, creatinine and cholesterol concentrations were lower (P 0:10) in calculated insulin sensitivity index was found after IAGTT between breeds. A time response (P, This research was supported by grants no. AGL 2006-05937/GAN and AGL 2009-08916 from Ministerio de Educación y Ciencia (Spain) and RECUPERA 2020, FEDER funding). There was no additional external funding received for this study. The publication fee was supported by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Nowadays, we know thousands of exoplanets, some of them potentially habitable. Next technological facilities (JWST, for example) have exoplanet atmosphere analysis capabilities, but they also have limits in terms of how many targets can be studied. Therefore, there is a need to rank and prioritize these exoplanets with the aim of searching for biomarkers. Some criteria involved, such as the habitability potential of a dry-rock planet versus a water-rich planet, or a potentially-locked planet versus a tidally-locked planet, are often vague and the use of the fuzzy set theory is advisable. We have applied a combination of Multi-Criteria Decision-Making methodologies with fuzzy logic, the Fuzzy Reference Ideal Method (FRIM), to this problem. We have analyzed the habitability potential of 1798 exoplanets from TEPCat database based on a set of criteria (composition, atmosphere, energy, tidal locking, type of planet and liquid water), in terms of their similarity to the only ideal alternative, The Earth. Our results, when compared with the probability index SEPHI, indicate that Kepler-442b, Kepler-062e/f, and LHS_1140b are the best exoplanets for searching for biomarkers, regardless its technical difficulty. If we take into account current technical feasibility, the best candidate is TRAPPIST-1e.

Two algorithms are introduced for the computation of discrete integral transforms with a multiscale approach operating in discrete three-dimensional (3-D) volumes while considering its real-time implementation. The first algorithm, referred to as 3-D discrete Radon transform of planes, will compute the summation set of values lying in discrete planes in a cube that imitates, in discrete data, the integrals on two-dimensional planes in a 3-D volume similar to the continuous Radon transform. The normals of these planes, equispaced in ascents, cover a quadrilateralized hemisphere and comprise 12 dodecants. The second proposed algorithm, referred to as the 3-D discrete John transform of lines, will sum elements lying on discrete 3-D lines while imitating the behavior of the John or x-ray continuous transform on 3-D volumes. These discrete integral transforms do not perform interpolation on input or intermediate data, and they can be computed using only integer arithmetic with linearithmic complexity, thus outperforming the methods based on the Fourier slice-projection theorem for real-time applications. We briefly prove that these transforms have fast inversion algorithms that are exact for discrete inputs.

Suppression of the recA SOS response gene and reactive oxygen species (ROS) overproduction have been shown, separately, to enhance fluoroquinolone activity and lethality. Their putative synergistic impact as a strategy to potentiate the efficacy of bactericidal antimicrobial agents such as fluoroquinolones is unknown. We generated Escherichia coli mutants that exhibited a suppressed ΔrecA gene in combination with inactivated ROS detoxification system genes (ΔsodA, ΔsodB, ΔkatG, ΔkatE, and ΔahpC) or inactivated oxidative stress regulator genes (ΔoxyR and ΔrpoS) to evaluate the interplay of both DNA repair and detoxification systems in drug response. Synergistic sensitization effects, ranging from 7.5- to 30-fold relative to the wild type, were observed with ciprofloxacin in double knockouts of recA and inactivated detoxification system genes. Compared to recA knockout, inactivation of an additional detoxification system gene reduced MIC values up to 8-fold. In growth curves, no growth was evident in mutants doubly deficient for recA gene and oxidative detoxification systems at subinhibitory concentrations of ciprofloxacin, in contrast to the recA-deficient strain. There was a marked reduction of viable bacteria in a short period of time when the recA gene and other detoxification system genes (katG, sodA, or ahpC) were inactivated (using absolute ciprofloxacin concentrations). At 4 h, a bactericidal effect of ciprofloxacin was observed for ΔkatG ΔrecA and ΔahpC ΔrecA double mutants compared to the single ΔrecA mutant (Δ3.4 log(10) CFU/ml). Synergistic quinolone sensitization, by targeting the recA gene and oxidative detoxification stress systems, reinforces the role of both DNA repair systems and ROS in antibiotic-induced bacterial cell death, opening up a new pathway for antimicrobial sensitization.

The prognosis of MEN 1 patients is not only determined by pancreatic disease; it is also related to other uncommon tumors. The objective of this study is to analyze the tumors associated with MEN 1 outside the classic triad and to investigate their relationship with mortality.One hundred and five MEN 1 patients were studied in a tertiary referral hospital (1980-2019).With a follow-up of 11 ± 4 years, seven patients died (8%), four as a consequence MEN syndrome. Thirty-three percent had adrenal gland tumors. One patient died of adrenal cancer. Eight percent presented with a neuroendocrine thoracic neoplasm, and one patient died. Another patient died due to cutaneous T-cell lymphoma. A further patient died because of a gastrinoma with liver metastasis.To conclude, 75% of MEN-related deaths were the result of an uncommon pathology, and we, therefore, recommend that these tumors should be taken into account in the screening and follow-up of these patients.

In this paper we introduce a new metrology technique for measuring wafer geometry on silicon wafers. Wave Front Phase Imaging (WFPI) has high lateral resolution and is sensitive enough to measure roughness on a silicon wafer by simply acquiring a single image snapshot of the entire wafer. WFPI is achieved by measuring the reflected light intensity from monochromatic uncoherent light at two different planes along the optical path with the same field of view. We show that the lateral resolution in the current system is 24μm though it can be pushed to less than 5μm by simply adding more pixels to the image sensor. Also, we show that the amplitude resolution limit is 0.3nm. A 2-inch wafer was measured while laying on a flat sample holder and the roughness was revealed by applying a double Gaussian high pass filter to the global topography data. The same 2-inch wafer was also placed on a simulated robotic handler arm, and we show that even if gravity was causing extra bow on the wafer, the same roughness was still being revealed at the same resolution after a high pass filter was applied to the global wafer geometry data.

Diffuse intrinsic pontine gliomas (DIPGs) are the most fatal primary brainstem tumors in pediatric patients. The identification of new molecular features, mediating their formation and progression, as non-coding RNAs (ncRNAs), would be of great importance for the development of effective treatments.We analyzed the DIPGs transcriptome with the HTA2.0 array and it was compared with pediatric non-brainstem astrocytoma expression profiles (GSE72269).More than 50% of the differentially expressed transcripts were ncRNAs and based on this, we proposed a DIPGs ncRNA signature. LncRNAs XIST and XIST-210, and the HBII-52 and HBII-85 snoRNA clusters were markedly downregulated in DIPGs. qPCR assays demonstrated XIST downregulation in all non-brainstem astrocytomas, in a gender, age, and brain location-independent manner, as well as in DIPGs affecting boys; however, DIPGs affecting girls showed both downregulation and upregulation of XIST. Girls' with longer survival positively correlated with XIST expression.The involvement of ncRNAs in DIPGs is imminent and their expression profile is useful to differentiate them from non-neoplastic tissues and non-brain stem astrocytomas, which suggests their potential use as DIPG biomarkers. In fact, XIST and XIST-210 are potential DIPG prognostic biomarkers.

The interaction between the Cu(II) ions and heterocyclic herbicides (triazines and imidazolinones) is studied by electrochemical and spectrophotometric measurements in relation to the environmental consequences of this interaction. The formation of very stable complexes between the herbicides and the metal ions is assessed. Due to the occurrence of acid–base reactions, the stability constants of the complexes were obtained at three pH values for the imidazolinone herbicides and at only in neutral media for triazine herbicides. Imidazolinones form 1:1 complexes in strongly acidic media and 1:2 complexes (one copper ion and two herbicide molecules) in the rest of pH values studied. Triazine herbicides form 1:2 complexes. The complexes formed in the pH range 5–10 (that is, the pH of most soils) are more stable for triazines than for imidazolinones, though in both cases this complex formation increases the low solubilities of the herbicides in water. This can lead to a decrease in the persistence of the herbicides and an increase in the ability of both the herbicides and the Cu(II) ions to reach groundwater and, consequently, to unwanted areas of crops.

We have undertaken a comprehensive search for both Lyman Alpha Emitters (LAEs) and Lyman Break Galaxies (LBGs) in the SHARDS Survey of the GOODS-N field. SHARDS is a deep imaging survey, made with the 10.4 m Gran Telescopio Canarias (GTC), employing 25 medium band filters in the range from 500 to 941 nm. This is the first time that both LAEs and LBGs are surveyed simultaneously in a systematic way in a large field. We draw a sample of 1558 sources; 528 of them are LAEs. Most of the sources (1434) show rest-frame UV continua. A minority of them (124) are pure LAEs with virtually no continuum detected in SHARDS. We study these sources from $z\sim3.35$ up to $z\sim6.8$, well into the epoch of reionization. Note that surveys done with just one or two narrow band filters lack the possibility to spot the rest-frame UV continuum present in most of our LAEs. We derive redshifts, Star Formation Rates (SFRs), Ly$\alpha$ Equivalent Widths (EWs) and Luminosity Functions (LFs). Grouping within our sample is also studied, finding 92 pairs or small groups of galaxies at the same redshift separated by less than 60 comoving kpc. In addition, we relate 87 and 55 UV-selected objects with two known overdensities at $z=4.05$ and $z=5.198$, respectively. Finally, we show that surveys made with broad band filters are prone to introduce many unwanted sources ($\sim20$% interlopers), which means that previous studies may be overestimating the calculated LFs, specially at the faint end., Comment: Accepted for publication in MNRAS. 18 pages, 19 figures

Mrk\,622 is a Compton Thick AGN and a double-peaked narrow emission line galaxy, thus a dual AGN candidate. In this work, new optical long-slit spectroscopic observations clearly show that this object is rather a triple peaked narrow emission line galaxy, with both blue and red shifted narrow emission lines, as well as a much narrower emission line centred at the host galaxy systemic velocity. The average velocity offset between the blue and red shifted components is $\sim$500 km\,s$^{-1}$, which is producing the apparent double-peaked emission lines. These two components are in the loci of AGN in the Baldwin, Phillips \& Terlevich (BPT) diagrams and are found to be spatially separated by $\sim$76 pc. Analysis of the optical spatially resolved spectroscopic observations presented in this work favours that Mrk\,622 is a system consisting of a Composite AGN amidst a binary AGN candidate, likely the result of a recent merger. This notwithstanding, outflows from a starburst, or single AGN could also explain the triple nature of the emission lines., Comment: 5 pages, 3 figures, 3 tables. Accepted in MNRAS Letters. 5 pages, 3 tables, 3 figures

In the optimal design of experiments setup, different optimality criteria can be considered depending on the objectives of the practitioner. One of the most used is c-optimality, which for a given model looks for the design that minimizes the variance of the linear combination of the parameters’ estimators given by vector c. c-optimal designs are needed when dealing with standardized criteria, and are specially useful when c is taken to be each one of the Euclidean vectors since in that case they provide the best designs for estimating the individual parameters. The well known procedure proposed by Elfving for independent observations is the origin of the procedure that can be used in the correlation framework. Some analytical results are shown for the model with constant covariance, but even in this case the computational task can become quite hard. For this reason, an algorithmic procedure is proposed; it can be used when dealing with a general model and some covariance structures.

A new Statistical-likelihood Exo-Planetary Habitability Index (SEPHI) is presented. It has been developed to cover the current and future features required for a classification scheme disentangling whether any discovered exoplanet is potentially habitable compared with life on Earth. The SEPHI uses likelihood functions to estimate the habitability potential. It is defined as the geometric mean of four sub-indexes related with four comparison criteria: Is the planet telluric?; Does it have an atmosphere dense enough and a gravity compatible with life?; Does it have liquid water on its surface?; Does it have a magnetic field shielding its surface from harmful radiation and stellar winds?. Only with seven physical characteristics, can the SEPHI be estimated: Planetary mass, radius, and orbital period; stellar mass, radius, and effective temperature; planetary system age. We have applied the SEPHI to all the planets in the Exoplanet Encyclopaedia using a Monte Carlo Method. Kepler-1229 b, Kepler-186 f, and Kepler-442 b have the largest SEPHI values assuming certain physical descriptions. Kepler-1229 b is the most unexpected planet in this privileged position since no previous study pointed to this planet as a potentially interesting and habitable one. In addition, most of the tidally locked Earth-like planets present a weak magnetic field, incompatible with habitability potential. We must stress that our results are linked to the physics used in this study. Any change in the physics used only implies an updating of the likelihood functions. We have developed a web application allowing the on-line estimation of the SEPHI: http://sephi.azurewebsites.net/, 10 pages, 4 figures, 6 tables. Accepted for publication in MNRAS

Several studies of nearby active galactic nuclei (AGN) have shown that the soft X-ray emission presents a size and morphology that resembles that of the narrow-line region (NLR) traced by [O III]. Since the NLR is mainly constituted by gas photoionised by the AGN, it seems logical to assume that this is also the primary source of the soft X-ray emission. However, these results are based on individual sources or small samples, particularly focused on type-2 Seyfert galaxies. Very little has been said concerning other types of AGN. The purpose of this work is to compare the circumnuclear morphologies of soft X-ray and [O III] images to test whether they match in different optical classes of AGN. Our sample is composed of 27 AGN: nine type-1 Seyferts, 10 type-2 Seyferts, and eight low ionisation nuclear emission-line regions (LINERs). We find a good match in 100% of the type-2 Seyferts in our sample. This correspondence is less frequent in type-1 Seyferts (22%) and it is not seen in LINERs. The good resemblance in type-2 Seyferts constitutes an evidence for a common physical origin. We argue that the lack of correspondence in type-1 Seyferts might be due to the line of sight perpendicular to the accretion disk. Based on the morphologies of the eight LINERs in our sample, we discard a common origin for the soft X-ray and [O III] emissions in these objects. Regarding the X-ray properties, both high column density and hard X-ray luminosity are associated with matched morphologies., Accepted by MNRAS. 20 pages, 9 figures

Six glassy carbon electrodes modified with polyphenazine conducting polymers and metallic nanoparticles have been prepared by using electrochemical techniques with the aim of determining antioxidant capacity. The electrodes showed a cathodic peak corresponding to the H 2 O 2 reduction, being adequate for the quantitative studies of this compound. These electrodes can be used to study the interaction between H 2 O 2 and antioxidants. From this interaction it was measured the antioxidant capacity of ascorbic acid. This capacity, in trolox equivalents, was similar for all electrodes studied, and agreed with that previously reported using other contrasted techniques. Platinum nanoparticles modified electrodes are best from the viewpoint of durability but, provided the lower cost of silver salts, silver nanoparticles modified electrodes are also a good selection, though these electrodes must be prepared daily.

The differences between the inherent stellar populations (SPs) of LAEs and LBGs are a key factor in understanding early galaxy formation and evolution. We have run a set of SP burst-like models for a sample of 1,558 sources at $3.4, Comment: Accepted for publication in MNRAS. 18 pages, 23 figures

We show herein that a proto-cluster of Ly α emitting galaxies, spectroscopically confirmed at redshift 6.5, produces a remarkable number of ionizing continuum photons. We start from the Ly α fluxes measured in the spectra of the sources detected spectroscopically. From these fluxes, we derive the ionizing emissivity of continuum photons of the protocluster, which we compare with the ionizing emissivity required to reionize the protocluster volume. We find that the sources in the protocluster are capable of ionizing a large bubble, indeed larger than the volume occupied by the protocluster. For various calculations, we have used the model AMIGA, in particular to derive the emissivity of the Lyman continuum photons required to maintain the observed volume ionized. Besides, we have assumed the ionizing photons escape fraction given by AMIGA at this redshift., With funding from the Spanish government through the "María de Maeztu Unit of Excellence" accreditation (MDM-2017-0737)

Although the incidence of papillary thyroid microcarcinoma (PTMC) has increased in recent decades, the role played by minimal extrathyroidal extension (mETE) in the prognosis of PTMC is still unclear. The aim of this study is to analyse the factors associated with PTMC and mETE and the long-term prognosis of PTMC.We conducted a retrospective study with a population consisting of patients with a histological diagnosis of PTMC. We excluded patients who had previously undergone thyroid surgery, those who had other synchronous malignancies, those with an ectopic location of the PTMC and those lost to follow-up within 2years. We compared group 1 (PTMC without extrathyroidal extension) versus group 2 (PTMC with mETE) and performed a multivariate analysis.We observed PTMC with mETE in 11.2% (n=18) of the patients. In the multivariate analysis, mETE was associated with an age ≥45 years (OR, 4.383; 95% CI 1.051-18.283, p=.043), a tumour size ≥8mm (OR, 5.913; 95% CI 1.795-19.481; p=.003), bilaterality (OR, 4.430; 95% CI 1.294-15.173; p=.018) and metastatic lymph nodes (OR, 12.588; 95% CI 2.919-54.280; p=.001). During a mean follow-up of 119.8±65 months, one recurrence was detected in group 2 (0% vs. 5.6%; p=.112), but none of the patients died due to the disease. Disease-free survival was lower in group 2 (124.9±5.6 vs. 97.4±10.3 months; p=.034).The mETE of MCPT is a factor of worse prognosis, associated with the presence of metastatic lymph nodes and lower disease-free survival.

We present subarcsecond 7.5-13 μm imaging- and spectro-polarimetric observations of NGC 1068 using CanariCam on the 10.4-m Gran Telescopio CANARIAS. At all wavelengths, we find: (1) A 90 × 60 pc extended polarized feature in the northern ionization cone, with a uniform ~44° polarization angle. Its polarization arises from dust and gas emission in the ionization cone, heated by the active nucleus and jet, and further extinguished by aligned dust grains in the host galaxy. The polarization spectrum of the jet-molecular cloud interaction at ~24 pc from the core is highly polarized, and does not show a silicate feature, suggesting that the dust grains are different from those in the interstellar medium. (2) A southern polarized feature at ~9.6 pc from the core. Its polarization arises from a dust emission component extinguished by a large concentration of dust in the galaxy disc. We cannot distinguish between dust emission from magnetically aligned dust grains directly heated by the jet close to the core, and aligned dust grains in the dusty obscuring material surrounding the central engine. Silicate-like grains reproduce the polarized dust emission in this feature, suggesting different dust compositions in both ionization cones. (3) An upper limit of polarization degree of 0.3 per cent in the core. Based on our polarization model, the expected polarization of the obscuring dusty material is ≲ 0.1 per cent in the 8-13 μm wavelength range. This low polarization may be arising from the passage of radiation through aligned dust grains in the shielded edges of the clumps., AA-H acknowledges financial support from the Spanish Ministry of Economy and Competitiveness (MINECO) under the 2011 Severo Ochoa Program MINECO SEV-2011- 0187. AA-H acknowledges financial support from the Spanish Ministry of Economy and Competitiveness through grant AYA2012-31447, which is partly funded by the FEDER program, PE from grant AYA2012-31277, and LC from grant AYA2012-32295. RN acknowledges support by FONDECYT grant no. 3140436. CRA is supported by a Marie Curie Intra European Fellowship within the 7th European Community Framework Programme (PIEF-GA-2012-327934). EP acknowledges support from NSF grant AST-0904896.

Solar systems complexity, multiscale, and nonlinearity are governed by numerous and continuous changes where the sun magnetic fields can successfully represent many of these phenomena. Thus, nonlinear tools to study these challenging systems are required. The dynamic system recurrence approach has been successfully used to deal with this kind challenge in many scientific areas, objectively improving the recognition of state changes, randomness, and degrees of complexity that are not easily identified by traditional techniques. In this work we introduce the use of these techniques in photospheric magnetogram series. We employ a combination of recurrence quantification analysis with a preprocessing denoising wavelet analysis to characterize the complexity of the magnetic flux emergence in the solar photosphere. In particular, with the developed approach, we identify regions of evolving magnetic flux and where they present a large degree of complexity, i.e., where predictability is low, intermittence is high, and low organization is present.

In this work we present a novel wave front phase sensing technique developed by Wooptix. This new wave front phase sensor uses only standard imaging sensor, and does not need any specialized optical hardware to sample the optical field. In addition, the wave front phase recovery is zonal, thus, the obtained wave front phase map provides as much height data points, as pixels in the imaging sensor. We will develop the mathematical foundations of this instrument as well as theoretical and practical limits. Finally, we will expose the application of this sensor to silicon wafer metrology and comparisons against industry standard metrology instruments.

A severe basal rot of leek (Allium porrum L.) was first observed in 1996 in Sueca (Valencia) eastern Spain, and again from 1998 to 2000 in several fields in Haro (La Rioja) northern Spain, where the disease caused significant economic losses. Leeks were stunted, wilted, and had water-soaked tissues with a characteristic reddish purple discoloration at the base of the stem. Affected plants could be pulled up easily because of rotting of the basal plate and root system. Similar symptoms were also observed on seedlings 2 to 3 weeks after transplanting. Isolations from symptomatic stems and roots onto potato dextrose agar (PDA) supplemented with 0.5 mg/ml of streptomycin sulfate (PDAS) consistently yielded a Fusarium sp. Isolates were transferred to potato sucrose agar (PSA) and synthetic low nutrient agar (SNA) and incubated at 25°C for 10 days with a 12-h photoperiod. The isolates were identified as Fusarium culmorum (Wm. G. Sm.) Sacc. based on colony morphology on PSA and phialide and conidial morphology on SNA. This disease has been described on garlic (1), but there are few reports about its incidence on leek (2). Pathogenicity tests were conducted on leek cvs. Alora, Axel, Casado, Goliath, and Varea using five isolates of F. culmorum from different locations. Ninety-day-old seedlings were inoculated by introducing a spore suspension to obtain a soil infested with the fungus at 104 colony-forming units (CFU)/g of potting mix (a sterilized mixture of equal portions [v/v] of soil, sand, and peat moss). Plants were maintained in a greenhouse at 20 to 30°C. Within 45 to 70 days after inoculation, symptoms developed that were similar to those observed in the field. The fungus was reisolated from affected plants, completing Koch's postulates. All isolates were pathogenic, and all leek cvs. were highly susceptible to the pathogen. To our knowledge, this is the first report of F. culmorum affecting leek in Spain. References: (1) F. J. Crowe et al. Phytopathology 76:1094, 1986. (2) G. Tamietti and A. Garibaldi. Riv. di Patol. Veg. IV. 13:69, 1977.

Background:Patients with inflammatory rheumatic diseases (IRD) infected with SARS-CoV-2 may be at risk to develop a severe course of COVID-19 due to the immune dysregulation or the influence of immunomodulating drugs on the course of the infection. For a better understanding of SARS-CoV-2 infections in patients with IRD and due to the high incidence of COVID-19 in Madrid from the beginning of this pandemic infection in Spain, the Society of Rheumatology from Madrid (SORCOM) established a registry (REUMA-COVID SORCOM) shortly after the beginning of the pandemic in Spain.Objectives:To determine factors associated with severity of infection with SARS-CoV-2 in patients with inflammatory rheumatic diseases in MadridMethods:The REUMA-COVID SORCOM registry is a multicenter, retrospective, observational cohort study conducted in Madrid, a SORCOM initiative. All rheumatology departments from Madrid were invited to participate. The study includes patients with IRD presenting with a confirmed or highly suspected diagnosis of COVID-19 between March 1, 2020, and November 10, 2020. We consider severe infection death or need of hospitalization. Inclusion criteria was having an IRD and at least 1 of the following 4 criteria: (1) a biologically confirmed COVID-19 diagnosis based on a positive result of a SARS-CoV-2 polymerase chain reaction (PCR) test on a nasopharyngeal swab; (2) Detection of IgM or IgG anti SARS-CoV2 in a symptomatic or asymptomatic patients (3)typical thoracic computed tomography (CT) abnormalities (ground-glass opacities) in epidemic areas; (4) COVID19–typical symptoms in an epidemic zone of COVID-19.Results:As of November 10, 2020, 417 patients with IRD were included in the REUMA-COVID SORCOM registry. 5 patients were discharged for incomplete data. Of 412 patients (mean age 57 years, 87.4% Caucasian race, 66.3% female) 174 need hospitalization (42.2%) and 33 patients died (18.4% mortality in hospitalized patients). 82.3% had comorbidities. 234 (56.8%) patients were classified as inflammatory arthropathy, 133 (32.3%) had connective tissue diseases (CTD). 41.1% of the patients had a large history of IRD (> 10 years). 10.4% of patients had previously pulmonary involvement. The study includes 143 patients taking Methotrexate, 89 patients taking anti-TNFα therapy and 27 Rituximab. In the univariant analysis, no differences were seen in the severity of COVID-19 infection in patients taking methotrexate. 63% of the all patients taking Rituximab included in the registry need hospitalization and 22% of them died. Hypertension, COPD or cardiovascular disease was associated with hospitalization.Independent factors associated with COVID-19 hospitalization in the multivariate analysis was: age (>62 years), male sex, IMC >30, previous cardiovascular comorbidities and the IRD disease duration (> 10 years). Independent factors associated with COVID-19 related death was: age (> 62 years), having a CTD diagnose, pulmonary involvement before infection and chronical GC treatment.Conclusion:Patients with IRD represent a population of particular interest in the pandemic context because the baseline immunological alteration and the treated with immunosuppressants agents they receive, comorbidities and the well-known risk of severe infection. Older age, male sex, cardiovascular comorbidities were factors associated with high risk of hospitalization in IRD patients. CTD diseases, previously pulmonary involvement and chronical GC treatment with more than 10mg/day were associated with high risk of death. Neither anti TNF-α treatment nor Methotrexate were risk factor for hospitalization or death COVID-19 related in IRD patients.Disclosure of Interests:None declared

Metal cutting is one of the most common metal-shaping processes. In this process, specified geometrical and surface properties are obtained through the break-up and removal of material by a cutting edge into a chip. The chip formation is associated with large strains, high strain rates and locally high temperatures due to adiabatic heating. These phenomena together with numerical complications make modeling of metal cutting challenging. Material models, which are crucial in metal-cutting simulations, are usually calibrated against data from material testing. Nevertheless, the magnitudes of strains and strain rates involved in metal cutting are several orders of magnitude higher than those generated from conventional material testing. Therefore, a highly desirable feature is a material model that can be extrapolated outside the calibration range. In this study, a physically based plasticity model based on dislocation density and vacancy concentration is used to simulate orthogonal metal cutting of AISI 316L. The material model is implemented into an in-house particle finite-element method software. Numerical simulations are in agreement with experimental results for different cutting speed and feed.