Search

Using dormant buds (DB) for germplasm cryopreservation was published few decades ago and since then, dormant buds have been used to preserve genetic resources of selected horticultural woody plant species. The advantages of employing DB in preservation are widely known; the most relevance is no requirements of aseptic cultures, high processing throughput and involvement of a relatively low skilled technical support; but the method has also shortcomings, such as seasonality of processing and lack of procedural modifications that might support preservation of all accessions in a collection. The US, NLGRP cryopreserved DB of 2168 unique Malus (Mill.) accessions (among 51 species) with a ≥40% post cryo viability. The method worked well for all accessions in 20 species, for ≥90% of accessions in six species and at a various percent (0-89%) in the remaining 25 species. For species with the largest number of processed accessions, the M. domestica, Borkh., M. hybrid and M. sieversii (Ledeb.) M. Roem. (1355, 326 and 128 cryopreserved accessions, respectively), the percent of accessions responding favorable to the DB method was high (96, 96 and 82%). The results indicated variability in Malus DB response to liquid nitrogen exposure; similar results were recorded in the Pyrus L. collections. Procedural refinements of the DB cryopreservation method could increase the method applicability to a much higher number of Malus species and its use in other collections of horticultural woody plants like Prunus L. and Pyrus L.

We present the first optical photometry of the counterpart to the candidate intermediate polar RX J0153.3+7446. This reveals an optical pulse period of 2333s +/- 5s. Reanalysis of the previously published ROSAT X-ray data reveals that the true X-ray pulse period is probably 1974s +/- 30s, rather than the 1414 s previously reported. Given that the previously noted orbital period of the system is 3.94 h, we are able to identify the X-ray pulse period with the white dwarf spin period and the optical pulse period with the rotation period of the white dwarf in the binary reference frame, as commonly seen in other intermediate polars. We thus confirm that RX J0153.3+7446 is indeed a typical intermediate polar., 4 pages, submitted to A&A Letters