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Sample collection activities for a study of population genetics across the natural distribution of targeted tree species require a lot of resources, mainly if repeated field visits are necessary. Conventionally, population genetic studies use good sample material like leaves. In addition, cambium or small pieces of fresh wood can be used to replace leaf samples. Currently, restrictions from the permit regulation have caused only a limited number of samples that can be collected. Therefore, efficient use of samples must be designed to maximize their uses for research. Due to the small amount of successfully sampled materials, hence there are limitations to extend their uses for other analyses and are often sufficient only for genetic analysis. Therefore, innovation in sampling methods using pickering punch (https://www.agroisolab.com/pickering-punch) to collect ebony wood cores in this study is required to cover multi-analyses not only limited to genetics but also for other analyses such as isotopes, near-infrared spectroscopy (NIRs), anatomy, and chemical compounds.•Pickering punch is recommended for efficient wood core sample collection from ebony standing trees.•323 wood core samples were successfully collected from 16 natural populations across Celebes (Sulawesi).•Multi-analyses studies on sampled wood cores are possible for ebony wood identification (e.g., species and origin/provenance).

Ebony (Diospyros celebica Bakh) is an endemic forest tree species found in Sulawesi whose basic information on its metabolite profile is still lacking. The objective of this research was to separate and identify putatively metabolite present in the leaves and wood of ebony across Sulawesi. Separation and identification of ebony metabolites were carried out using UHPLC-Q-Orbitrap HRMS analysis. Using ultrasonication with ethanol as the extracting solvent, we used powdered ebony leaves and wood. The results showed that the metabolites contained in the leaves and wood of ebony were 59 metabolites. About 14 compounds were found in the leaves and wood, 21 compounds in the wood, and 24 compounds in the leaves. The identified metabolites are flavonoids, terpenoids, amino aldehydes, alkaloids, quinones, steroids, amino acids, fatty acids, and saccharides. Clustering of ebony using principal component analysis obtained leaves and wood groups using peak area of known compounds as the variable.

Community forest development requires a constant supply of high-quality seeds. In addition, sound management of Toona sinensis (surian) seed sources requires a deep understanding of factors affecting seed production. This present study investigated the reproduction characteristics of surian, including flower, fruit and seed productions, variations in the productions of fruits and seeds among trees and among branches, and dendrometric factors that influence the productions of fruits and seeds. Flower production characteristics were observed in 99 panicles, fruit production characteristics were observed in 128 panicles, and seed characteristics were evaluated based on 890 fruits. The number of fruits per panicle ranged from 38 to 646. The number of seeds in fruits ranged from 1 to 35. Fruit size was correlated to the number of filled seeds following a quadratic regression equation. The optimal number of filled seeds was 20 per fruit. Stem diameter, crown width, crown base height, and the number of sub-branches positively influenced the production of panicles per tree, while the crown base height (of the tree) negatively affected the fruit set.Pembangunan hutan rakyat memerlukan bekalan tetap benih berkualiti tinggi. Selain itu, pengurusan sumber benih

Siregar IZ, Yunanto T. (2010). The genetics of glutamate oxaloacetate transaminase (GOT) in Pinus merkusii Jungh. et de Vriese. Biodiversitas 11: 5-8. Inheritance and linkage analysis of glutamate oxalacetate transaminase (GOT) in P. merkusii were performed using megagametophyte tissues of single tree seed-lots. One monomorphic locus (GOT-A) and three polymorphic loci (GOT-B,GOT-C and GOT-D) were identified, based on the allelic 1: 1 segregation of putatively heterozygous trees. Linkage analysis revealed that GOTC and GOT-D were closely linked loci with an average recombination rate (R) of about 5%. This result which is in contrast to the GOT genetics in other pine species was discussed with regard to the probably occurring gene duplication of one GOT locus.