25 results on '"Ibrahim Harbi"'
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2. Low-Complexity Finite Set Model Predictive Control for Split-Capacitor ANPC Inverter With Different Levels Modes and Online Model Update
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Ibrahim Harbi, Mostafa Ahmed, Jose Rodriguez, Ralph Kennel, and Mohamed Abdelrahem
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Energy Engineering and Power Technology ,Electrical and Electronic Engineering - Published
- 2023
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3. Direct Power Control Based on Dead-beat Function and Extended Kalman Filter for PV Systems
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Mostafa Ahmed, Ibrahim Harbi, Ralph Kennel, and Mohamed Abdelrahem
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Renewable Energy, Sustainability and the Environment ,Energy Engineering and Power Technology - Published
- 2023
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4. An improved photovoltaic maximum power point tracking technique-based model predictive control for fast atmospheric conditions
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Mostafa Ahmed, Ibrahim Harbi, Ralph Kennel, Jose Rodriguez, and Mohamed Abdelrahem
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General Engineering - Published
- 2023
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5. A Nine-Level T-Type Converter for Grid-Connected Distributed Generation
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Ibrahim Harbi, Mostafa Ahmed, Jose Rodriguez, Ralph Kennel, and Mohamed Abdelrahem
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Energy Engineering and Power Technology ,Electrical and Electronic Engineering - Published
- 2022
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6. A Nine-Level Split-Capacitor Active-Neutral-Point-Clamped Inverter and Its Optimal Modulation Technique
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Ibrahim Harbi, Mostafa Ahmed, Christoph M. Hackl, Ralph Kennel, and Mohamed Abdelrahem
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Electrical and Electronic Engineering - Published
- 2022
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7. Model-Based Maximum Power Point Tracking Algorithm With Constant Power Generation Capability and Fast DC-Link Dynamics for Two-Stage PV Systems
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Mostafa Ahmed, Ibrahim Harbi, Ralph Kennel, Jose Rodriguez, and Mohamed Abdelrahem
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General Computer Science ,General Engineering ,General Materials Science ,Electrical and Electronic Engineering - Published
- 2022
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8. Enhanced Fault-Tolerant Robust Deadbeat Predictive Control for Nine-Level ANPC-Based Converter
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Ibrahim Harbi, Mostafa Ahmed, Marcelo Lobo Heldwein, Ralph Kennel, and Mohamed Abdelrahem
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General Computer Science ,General Engineering ,General Materials Science ,Electrical and Electronic Engineering - Published
- 2022
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9. Maximum power point tracking‐based model predictive control with reduced sensor count for PV applications
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Mostafa Ahmed, Ibrahim Harbi, Christoph M. Hackl, Ralph Kennel, Jose Rodriguez, and Mohamed Abdelrahem
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Renewable Energy, Sustainability and the Environment - Published
- 2022
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10. DC-link sensorless control strategy for grid-connected PV systems
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Ibrahim Harbi, Ahmed Farhan, Ralph Kennel, Mohamed Abdelrahem, and Mostafa Ahmed
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Computer science ,020209 energy ,Applied Mathematics ,020208 electrical & electronic engineering ,Photovoltaic system ,Particle swarm optimization ,02 engineering and technology ,Grid ,ddc ,Reduction (complexity) ,Reliability (semiconductor) ,Control theory ,Boost converter ,0202 electrical engineering, electronic engineering, information engineering ,Inverter ,Electrical and Electronic Engineering ,Voltage - Abstract
Sensorless strategies become very popular in modern control techniques because they increase the system reliability. Besides, they can be used as back-up control in case of sensor failure. In this paper, a DC-link sensorless control approach is developed, which is suited for grid-connected PV systems. The studied system is a two-stage PV scheme, where the DC–DC stage (boost converter) is controlled using an adaptive step-size perturb and observe (P&O) method. Further, the inverter control is accomplished by voltage oriented control (VOC). Generally, the VOC is implemented with two cascaded control loops, namely an outer voltage loop and an inner current loop. However, in this work, the outer loop is avoided and the reference current is generated using a losses model for the system. The losses model accounts for the most significant losses in the PV system. Moreover, particle swarm optimization (PSO) is utilized to compensate for the unmodeled losses. The PSO is executed offline for the purpose of calculation burden reduction. The proposed approach simplifies the cascaded VOC strategy and eliminates the DC-link voltage sensor, which in turn decreases the cost of the system. Finally, the proposed technique is compared with the conventional one at different atmospheric conditions and validated using MATLAB simulation results.
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- 2021
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11. Computationally Efficient FCS-MPC for Single-Phase Five-Level ANPC Inverter
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Ibrahim Harbi, Mohamed Abdelrahem, Mahmoud Aref, Mostafa Ahmed, and Ralph Kennel
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- 2021
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12. Robust Multiple-Vector Predictive Control for Power Converters with Grid-Voltage Estimation
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Mohamed Abdelrahem, Mostafa Ahmed, Ibrahim Harbi, Ralph Kennel, and Jose Rodriguez
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- 2021
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13. Finite Set Model Predictive Control for Split-Capacitor Active-Neutral-Point-Clamped Inverter with Different Voltage Levels Operating Modes
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Ibrahim Harbi, Mohamed Abdelrahem, Mostafa Ahmed, Ralph Kennel, and Jose Rodriguez
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- 2021
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14. Performance Evaluation of PV Model-Based Maximum Power Point Tracking Techniques
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Mostafa Ahmed, Ibrahim Harbi, Ralph Kennel, Marcelo Lobo Heldwein, José Rodríguez, and Mohamed Abdelrahem
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Computer Networks and Communications ,Hardware and Architecture ,Control and Systems Engineering ,Signal Processing ,Electrical and Electronic Engineering ,Review ,PV systems ,maximum power point tracking ,review ,PV model-based maximum power point tracking ,ddc - Abstract
Maximum power point tracking (MPPT) techniques extract the ultimate power from the photovoltaic (PV) source. Therefore, it is a fundamental control algorithm in any PV configuration. The research in this area is rich and many MPPT methods have been presented in the literature. However, in the current study, we focus on the PV model-based MPPT algorithms. In this regard, the classification of this category can be mainly divided into curve fitting methods and techniques based on the mathematical model or characteristics of the PV source. The objective of the PV model-based MPPT algorithm is to allocate the position of the maximum power point (MPP). Thus, no searching efforts are required to capture that point, which makes it simple and easy to implement. Consequently, the aim of this study is to give an overview of the most commonly utilized model-based MPPT methods. Furthermore, discussion and suggestions are also addressed to highlight the gap in this area. The main methods from the literature are compared together. The comparison and evaluation are validated using an experimental hardware-in-the-loop (HIL) system, where high efficiency (more than 99%) can be obtained with a simple calculation procedure and fast convergence speed.
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- 2022
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15. Predictive Fixed Switching Maximum Power Point Tracking Algorithm with Dual Adaptive Step-Size for PV Systems
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Mostafa Ahmed, Ibrahim Harbi, Ralph Kennel, and Mohamed Abdelrahem
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TK7800-8360 ,Computer Networks and Communications ,predictive fixed switching frequency ,maximum power point tracking ,ddc ,PV systems ,finite set model predictive control ,switching frequency minimization ,cost function ,Hardware and Architecture ,Control and Systems Engineering ,Signal Processing ,Electrical and Electronic Engineering ,Electronics - Abstract
Maximum power point tracking (MPPT) is an essential and primary objective in photovoltaic (PV) systems implementation. Thus, in this article, the predictive fixed switching MPPT technique is proposed for a two-stage PV system, where the system under consideration consists of a PV source, boost converter, and two-level inverter. The MPPT design is based on dual adaptive step-size realization to limit the duty cycle oscillations at a steady state. Furthermore, the PI controller is eliminated, which simplifies the MPPT implementation. The suggested tuning procedure of the duty cycle is compared with the conventional adaptive step-size perturb and observe (P&O) method. The inverter is controlled using an efficient finite-set model predictive control (FS-MPC) algorithm with reduced computation burden, where the optimal switching state vector is identified based on the polarity of the reference voltage in the α-β reference frame and without any need for sector determination. Furthermore, the cost function of the FS-MPC algorithm is modified to include the reduction of the switching frequency as a secondary objective for the inverter control. The overall control methodology is evaluated using experimental results at different operating conditions.
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- 2020
16. Dual-Mode Power Operation for Grid-Connected PV Systems with Adaptive DC-link Controller
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Mohamed Abdelrahem, Mostafa Ahmed, Ralph Kennel, and Ibrahim Harbi
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Multidisciplinary ,Computer science ,020209 energy ,020208 electrical & electronic engineering ,Photovoltaic system ,PID controller ,02 engineering and technology ,Maximum power point tracking ,Research Article-Electrical Engineering ,PV systems ,Dual-mode power generation ,Adaptive DC-link control ,Predictive control ,ddc ,Electric power system ,Model predictive control ,Electricity generation ,Control theory ,0202 electrical engineering, electronic engineering, information engineering ,Voltage regulation - Abstract
Photovoltaic (PV) power systems are integrated with high penetration levels into the grid. This in turn encourages several modifications for grid codes to sustain grid stability and resilience. Recently, constant power management and regulation is a very common approach, which is used to limit the PV power production. Thus, this article proposes dual-mode power generation algorithm for grid-connected PV systems. The developed system considers the two-stage PV configuration for implementation, where the dual-mode power generation technique is executed within the DC–DC conversion (boost) stage. Most of the techniques adopted for dual-mode power operation employ the conventional perturb and observe method, which is known with unsatisfactory performance at fast-changing atmospheric conditions. Considering this issue, this study suggests a modified maximum power point tracker for power extraction. Furthermore, a new adaptive DC-link controller is developed to improve the DC-link voltage profile at different operating conditions. The adaptive DC-link controller is compared with the traditional PI controller for voltage regulation. The inverter control is accomplished using finite-set model predictive control with two control objectives, namely reference current tracking and switching frequency minimization. The overall control methodology is evaluated at different atmospheric and operating conditions using MATLAB/Simulink software.
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- 2020
17. Model Predictive Control with Switching Frequency Minimization for Modified Packed U-cell Inverter
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Mostafa Ahmed, Ralph Kennel, Mohamed Abdelrahem, and Ibrahim Harbi
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Model predictive control ,Control theory ,Computer science ,Control system ,Waveform ,Inverter ,Topology (electrical circuits) ,MATLAB ,computer ,Weighting ,computer.programming_language ,Voltage - Abstract
In this paper, a finite control set model predictive control (FCS-MPC) for single-phase cascaded modified packed U-cell (MPUC) inverter is presented. The considered multilevel inverter (MLI) topology requires less number of switches and produces more levels in the output waveform compared to the conventional and recent ones. Two control objectives are achieved in the proposed control algorithm, reference current tracking and switching frequency minimization. The proposed FCS-MPC considers only the effective different vectors of the MPUC with eliminating the redundant states of the converter to reduce the computational effort of the algorithm. By minimizing a user-defined cost function, the developed FCS-MPC algorithm achieves low mean absolute reference tracking error of the grid current, high inverter voltage quality and low average switching frequency of the switches. The trade-off between the average switching frequency and the tracking performance with the voltage quality is studied to determine the suitable range of the weighting factor λ n for the proposed system. Simulation results are presented using MATLAB/Simulink to verify the effectiveness of the control system.
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- 2020
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18. Evaluation of Predictive Direct Current and Direct Power Control for Grid-connected PV Systems
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Ibrahim Harbi, Mohamed Abdelrahem, Ralph Kennel, and Mostafa Ahmed
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Total harmonic distortion ,Model predictive control ,Computer science ,Control theory ,Photovoltaic system ,Boost converter ,Grid connection ,AC power ,Maximum power point tracking ,Power control - Abstract
Predictive control provides many advantages over conventional control techniques. Thus, in this paper, a comparative study between the predictive direct current control (PDCC) and predictive direct power control (PDPC) is investigated. The study focuses on applying these control strategies with renewable energy systems (RESs), especially photovoltaic (PV) ones. RESs are integrated with high penetration into the grid, and they must satisfy certain regulations to sustain the grid stability. The studied system consists of a PV array and boost converter followed by two-level inverter to enable the grid connection, where the maximum power point tracking (MPPT) function is accomplished by an adaptive step-size method. The adopted technique here is perturb and observe (P&O) method. The control of active and reactive power is performed using PDPC and PDCC for the purpose of evaluation in terms of power quality, total harmonic distortion (THD), average switching frequency, implementation, calculation burden, etc. The system is validated by simulation results via Matlab platform under various operating conditions.
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- 2020
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19. Simplified Model Predictive Current Control for Single-Phase Multilevel Inverter
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Christoph M. Hackl, Ibrahim Harbi, Ralph Kennel, and Mohamed Abdelrahem
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0209 industrial biotechnology ,Steady state (electronics) ,Computer science ,business.industry ,020208 electrical & electronic engineering ,Topology (electrical circuits) ,02 engineering and technology ,Function (mathematics) ,Condensed Matter::Mesoscopic Systems and Quantum Hall Effect ,Network topology ,Model predictive control ,020901 industrial engineering & automation ,Semiconductor ,Control theory ,0202 electrical engineering, electronic engineering, information engineering ,Transient (oscillation) ,business ,Voltage reference ,Voltage - Abstract
In this paper, an efficient finite control set model predictive control (FCS-MPC) for single-phase multilevel inverter (MLI) is proposed. The MLI topology under control reduces the number of the required semiconductor switches and produces more number of levels compared to traditional and recent topologies of MLIs. This topology has 49 different switching states, which means 49 predictions of the future current and 49 calculations of the cost function are required for each evaluation of the conventional FCS-MPC. Accordingly, the computational load is heavy. Thus, this paper presents a simplified FCS-MPC to reduce the calculation burden by computing the reference voltage and dividing the switching states of the MLI into two sets. Based on the reference voltage, one set is identified to determine the optimum voltage level. The proposed method does not require current predictions. Moreover, the number of cost function evaluations for each iteration is halved. As a result, the execution time is significantly reduced compared to that of the conventional FCS-MPC. The trade-off between the average switching frequency and the tracking performance is also investigated for the proposed scheme and compared to the conventional FCS-MPC. The effectiveness of the proposed solution in transient and steady state is verified by the simulation results.
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- 2020
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20. Maximum Power Point Tracking-Based Model Predictive Control for Photovoltaic Systems: Investigation and New Perspective
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Mostafa Ahmed, Ibrahim Harbi, Ralph Kennel, José Rodríguez, and Mohamed Abdelrahem
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PV systems ,MPPT ,review ,MPC ,sensor reduction ,fixed switching frequency ,direct MPPT ,Article ,Electrical and Electronic Engineering ,Biochemistry ,Instrumentation ,Atomic and Molecular Physics, and Optics ,ddc ,Analytical Chemistry - Abstract
In this paper, a comparative review for maximum power point tracking (MPPT) techniques based on model predictive control (MPC) is presented in the first part. Generally, the implementation methods of MPPT-based MPC can be categorized into the fixed switching technique and the variable switching one. On one side, the fixed switching method uses a digital observer for the photovoltaic (PV) model to predict the optimal control parameter (voltage or current). Later, this parameter is compared with the measured value, and a proportional–integral (PI) controller is employed to get the duty cycle command. On the other side, the variable switching algorithm relies on the discrete-time model of the utilized converter to generate the switching signal without the need for modulators. In this regard, new perspectives are inspired by the MPC technique to implement both methods (fixed and variable switching), where a simple procedure is used to eliminate the PI controller in the fixed switching method. Furthermore, a direct realization technique for the variable switching method is suggested, in which the discretization of the converter’s model is not required. This, in turn, simplifies the application of MPPT-based MPC to other converters. Furthermore, a reduced sensor count is accomplished. All conventional and proposed methods are compared using experimental results under different static and dynamic operating conditions.
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- 2022
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21. A higher levels multilevel inverter with reduced number of switches
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Haitham Z. Azazi, A. E. Lashine, Ibrahim Harbi, and Awad E. El-Sabbe
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Total harmonic distortion ,Computer science ,Filter (video) ,Multilevel inverter ,020208 electrical & electronic engineering ,0202 electrical engineering, electronic engineering, information engineering ,Electronic engineering ,020206 networking & telecommunications ,02 engineering and technology ,Electrical and Electronic Engineering ,Voltage - Abstract
This paper presents a high-levels multilevel inverter (MLI) with a reduced number of the required switches. Forty-nine levels can be obtained in the output voltage from this circuit by usin...
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- 2018
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22. An Adaptive Model-Based MPPT Technique with Drift-Avoidance for Grid-Connected PV Systems
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Ibrahim Harbi, Mostafa Ahmed, Mohamed Abdelrahem, and Ralph Kennel
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model-based MPPT ,Control and Optimization ,Maximum power principle ,Computer science ,020209 energy ,Energy Engineering and Power Technology ,PID controller ,grid-connection ,02 engineering and technology ,lcsh:Technology ,Maximum power point tracking ,PV systems ,adaptive step-size ,Control theory ,drift-avoidance ,0202 electrical engineering, electronic engineering, information engineering ,Grid connection ,Electrical and Electronic Engineering ,Engineering (miscellaneous) ,Renewable Energy, Sustainability and the Environment ,lcsh:T ,dead-beat predictive control ,020208 electrical & electronic engineering ,Photovoltaic system ,Power (physics) ,ddc ,Three-phase ,Inverter ,Pulse-width modulation ,Energy (miscellaneous) ,Voltage - Abstract
In this article, a modified control structure for a single-stage three phase grid-connected photovoltaic (PV) system is presented. In the proposed system, the maximum power point tracking (MPPT) function is developed using a new adaptive model-based technique, in which the maximum power point (MPP) voltage can be precisely located based on the characteristics of the PV source. By doing so, the drift problem associated with the traditional perturb and observe (P&O) technique can be easily solved. Moreover, the inverter control is accomplished using a predictive dead-beat function, which directly estimates the required reference voltages from the commanded reference currents. Then, the reference voltages are applied to a space vector pulse width modulator (SVPWM) for switching state generation. Furthermore, the proposed inverter control avoids the conventional and known cascaded loop structure of the voltage oriented control (VOC) method by elimination of the outer PI controller, and hence the overall control strategy is simplified. The proposed system is compared with different MPPT techniques, including the conventional P&O method and other techniques intended for drift avoidance. The evaluation of the suggested control methodology depends on various radiation profiles created in MATLAB. The proposed technique succeeds at capturing the maximum available power from the PV source with no drift in comparison with other methods.
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- 2019
23. Abdulkerîm Yahyâ ez-Z’ibâri’nin romanlarında realizm
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Ibrahim, Harbi Jalalaldin, Öncü, Mustafa, Dicle Üniversitesi, Sosyal Bilimler Enstitüsü, Temel İslam Bilimleri Anabilim Dalı, Arap Dili ve Belağatı Bilim Dalı, and Ibrahim, Harbi Jalalaldin
- Abstract
Bu çalışmada edebi roman alanında, yaratıcı Kürt yazarları arasında yer alan Abdulkerim Yahya Ez-Zibârî’nin romanlarında realizm ele alınmaktadır. Ez-Zibârî’nin romanlarına mükemmellik veren kullandığı sade ve akıcı Arapçadır. Yazarın romanlarından başka, yine Arapça olarak kaleme aldığı bazı kısa hikâyeler ve edebî eleştiri alanında birçok eseri bulunmaktadır. Yazar ulusal alanda birçok ödül kazanmıştır. Bunlardan biri Eşşarika Edebi Yaratıcılık ödülüdür. Bu çalışma yazarın iki romanı esas alınarak hazırlanmıştır. Uğniyât Et-tarîk İlâ Halepçe adlı romanında hayat realist bir bakış açısıyla ele alınmaktadır. Romanda yirminci yüzyıl başlarında, zorlu bir dönemde geçen Kürt toplumu ve diğer toplumlar ile bölgede yaşayan diğer milletler arasındaki yaşam imgelerinden bahsedilmektedir. Yazarın diğer romanı ise Şabbun ‘Arin Yahmilu Bundukiyyeteyn adını taşımaktadır. Bu romanda Irak’ın hem krallık hem de cumhuriyet dönemlerinde, zalim sistemler ve hükümetler altında acı çeken Kürt toplumunun yaşamından bahsedilmektedir. Bu romanlar, Irak’taki Kürt milletinin bu dönemlerde yaşadıklarını edebi bir bakış açısıyla yansıtmayı hedeflemiştir. Bu romanlarda Kürtlerin bölgedeki diğer Arap, Türk, Fars milletlerleriyle ilişkilerinden bahsedilmiştir. Bunun yanında bölgedeki aşiretlerin sıradan vatandaş üzerindeki zulmü, işkencesi ve baskısı yansıtılmaya çalışılmıştır. Burada ele alınan diğer bir konu da din adamlarının sömürge güçlerin emelleri karşısındaki siyasi tutumları ve rolüdür. Romanlarda Kürt toplumunda kadının statosü ve rolünden de bahsedilmektedir. Kürt toplumunda kadının konumuna bölgede yaşayan diğer milletlerin bakış açısı irdelenmiştir. Oryantalistlerin bu konudaki notları ve araştırmalarına değinilmiştir. Çalışmanın içerisinde bu sayılanlar ve daha fazlası yer almaktadır. Ez-Zibârî’nin romanları modern çağ sonrası dönemi temsil etmektedir. Zira yazar tarihi ve tarihi olayları modern bir bakış açısıyla, güçlü bir sanatsal örgü ve ifade biçimi ile okumaya çalışmaktadır. Yazar yerşeyi olduğu gibi gösteren bir fotoğraf makinesi gibi olmaktan kurtulmayı başarmıştır. Tam aksine tarihi gerçek olayları güzel tasvirler ve hayal gücüyle yansıtmıştır. Bunu da romanlarının edebi değerini yükselten bazı sanatsal edebi teknikler kullanarak yapmıştır. Bunların başında anlam/anlatım ötesi, hayal gücünü kullanma, metnin sembollerle dizimi, ironi ve retorik imgeler gibi edebi teknikler gelmektedir. This study attempts to stand and display the most significant features of realism in the novels of the Kurdish writer '' Abdul KarimYahyaZebari '', who is one of the Kurdish creative writersin the field ofliterature (Novel), besides, he has several books in criticism and short stories, who won international awards including the Sharjah Prize for Creativity Literary works at its twelfth session.The study displaysthe realistic aspect of his novel"Songs of the Road to Halabja", which reflects an image of life of Kurdish society with othercommunities in the region during a hard transitional period i.e. the beginning of twentieth century. While thenovel “A Disgrace Young Carries Two Guns” examines the sufferings of Kurdish societyunder the governments and tyrannical regimes, during the period of monarchy and republicanism in Iraq, where these stories went to deal with the history of Kurdish people in Iraq so as to reveal the reality in that historical period, in addition to the Kurds' relations with other communities whom lived together in the same region includingTurkmen,Persian, and Arab people. This novel also reveals the tribe’s tyrannical power and suppression to the common people, the role of religious men and their political movements against the ambitions of the colonial powers, moreover, the role of women in Kurdish society along with the people’s viewpoints towardsthem in the region, and the observations of orientalists and their studies on that status.All in all, throughout this study and what is observed from his novels, the study concluded that the novels are postmodern, where he tried to read the history in a post-modernistic viewpointby an artistic technique with high quality of performance, he endeavored to get rid of actual realism, such as the photographic device that depicts everything as it is, however,he combined history to his contemporary reality by his magic imagination and his beauty of photographing. The researchvia artistic literary techniques has examined in its study the "meta-narrative, fantasy, symbolic design, paradox and rhetorical images”.
- Published
- 2017
24. A Modified Modular Multilevel Converter with Reduced Number of Power Electronic Components
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Ibrahim Harbi, A. E. Lashine, Haitham Z. Azazi, and Awad E. El-Sabbe
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Total harmonic distortion ,Control theory ,Computer science ,Filter (video) ,visual_art ,Electronic component ,visual_art.visual_art_medium ,Inverter ,Waveform ,Topology (electrical circuits) ,Voltage ,Power (physics) - Abstract
This paper presents a modified multilevel inverter with a few number of semiconductorswitches and increasing number of output levels. One stage of the proposed inverter leads to getseven levels in the output voltage, while, forty-nine levels can be obtained when using two stages.Only six semiconductor switches are required for each stage. Standard DC sources are used, so, DC-DC converter is not required to get the desired output standard voltage. Total harmonic distortion in the output waveform of the two stage inverter is very low, so, using filter to improve output waveform is unnecessary. This topology is valid for all types of loads. Simulation results are presented for one stage and two stage inverters proposed. Experimental results are included for the one stage inverter to verify the theoretical results.
- Published
- 2015
- Full Text
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25. Glycosylated Sertraline-Loaded Liposomes for Brain Targeting: QbD Study of Formulation Variabilities and Brain Transport
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Ibrahim Harbi, Bader M Aljaeid, Khalid M. El-Say, and Ahmed S. Zidan
- Subjects
Chemistry, Pharmaceutical ,Pharmaceutical Science ,02 engineering and technology ,Aquatic Science ,Endocytosis ,Blood–brain barrier ,030226 pharmacology & pharmacy ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Drug Delivery Systems ,In vivo ,Live cell imaging ,Cell Line, Tumor ,Sertraline ,Drug Discovery ,medicine ,Animals ,Cytochalasin B ,Ecology, Evolution, Behavior and Systematics ,Liposome ,Glucose Transporter Type 1 ,Chromatography ,Ecology ,Chemistry ,Brain Neoplasms ,Brain ,Biological Transport ,General Medicine ,Glioma ,021001 nanoscience & nanotechnology ,medicine.anatomical_structure ,Transcytosis ,Blood-Brain Barrier ,Liposomes ,PEGylation ,Biophysics ,0210 nano-technology ,Agronomy and Crop Science - Abstract
Effectiveness of CNS-acting drugs depends on the localization, targeting, and capacity to be transported through the blood-brain barrier (BBB) which can be achieved by designing brain-targeting delivery vectors. Hence, the objective of this study was to screen the formulation and process variables affecting the performance of sertraline (Ser-HCl)-loaded pegylated and glycosylated liposomes. The prepared vectors were characterized for Ser-HCl entrapment, size, surface charge, release behavior, and in vitro transport through the BBB. Furthermore, the compatibility among liposomal components was assessed using SEM, FTIR, and DSC analysis. Through a thorough screening study, enhancement of Ser-HCl entrapment, nanosized liposomes with low skewness, maximized stability, and controlled drug leakage were attained. The solid-state characterization revealed remarkable interaction between Ser-HCl and the charging agent to determine drug entrapment and leakage. Moreover, results of liposomal transport through mouse brain endothelialpolyoma cells demonstrated greater capacity of the proposed glycosylated liposomes to target the cerebellar due to its higher density of GLUT1 and higher glucose utilization. This transport capacity was confirmed by the inhibiting action of both cytochalasin B and phenobarbital. Using C6 glioma cells model, flow cytometry, time-lapse live cell imaging, and in vivo NIR fluorescence imaging demonstrated that optimized glycosylated liposomes can be transported through the BBB by classical endocytosis, as well as by specific transcytosis. In conclusion, the current study proposed a thorough screening of important formulation and process variabilities affecting brain-targeting liposomes for further scale-up processes.
- Published
- 2015
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