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The study was aimed at investigating the concentration of macro/micro elements and proximate content of the fruit of Sarcocephalus latifolius with the view of validating its nutritional benefit to man. The plant material was collected from Gaya in Hong L.G.A of Adamawa State, Nigeria. Proximate analysis was conducted following methods of Association of Official Analytical Chemists. The percentage values of moisture, ash, protein, fibre and carbohydrate available were 14.6, 4.5, 8.52, 32.60 and 45.80 respectively. The levels of 13 elements (Ca, Mg, Na, K, P, S, Fe, Mn, Zn, Cu, Co, Cr, Ni) was determined using atomic absorption spectrophotometry. While the content of phosphorus and sulphur in the fruit was determined by colorimetry. Results revealed moderate to high concentrations of some macro and micro elements in the fruit of Sarcocephalus latifolius. Phosphorus was the highest amongst the macro element (0.531 g/100 g) which represent 37.96% of its Required Dietary Allowance (RDA). Magnesium (0.329 g/100 g), potassium (0.294 g/100 g) calcium (0.242 g/100 g) and sodium (0.196 g/100 g) which represent 16.40%, 7.34%, 34.80% and 17.80% of their required dietary allowance. Sulphur was the lowest (0.045 g/100 g) among the macro element. For the microelements, Zn, a potent antioxidant was the highest (0.019 g/100 g) which represents 87.27% of its required dietary allowance (RDA). While Mn and Fe showed 99.8% and 33.3%. In conclusion, both the proximate and elemental concentrations for the fruit of S. latifolius was found within the permissible region set by the World Health Organization.

The effects of WEB 2086 on human blood coagulation and fibrinolysis were studied in vitro using computerized thromboelastography and native whole blood. WEB 2086 (3.0, 30, 150, and 300 microM) had no apparent effect on the split point (SP), reaction time (R) and lysis of the clot. The biKoatugulierung time (K) was prolonged, the angle (alpha) and maximum amplitude (MA) were diminished, and the overall coagulation index (TEG index) was smaller. SP and R indicate onset of coagulation with the formation of fibrin strands, suggesting that WEB 2086 had no effect on the plasma factors. Since the Lysis parameter reflects fibrinolysis, WEB 2086 had no demonstrable fibrinolytic activity. K, alpha and MA involve platelet function and the integrity of fibrin; the fact that these factors were affected to the exclusion of SP and R suggests an effect largely on platelets. There was lack of a dose-response relationship at this concentration range (3.0-300 microM), probably due to saturation of PAF receptors even at the lowest effective concentration. These findings are consistent with the known effects of WEB 2086 on platelet function and suggest that thromboelastography may be a useful tool in the study of the activity of PAF receptor antagonists on coagulation profiles. Furthermore, since WEB 2086 is a highly specific PAF receptor antagonist devoid of intrinsic activity, and the samples used were not treated with exogenous PAF, these results suggest that endogenous PAF may play a significant role in the normal clotting mechanism.