47 results on '"Hui Yu Yang"'
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2. Effects of Visuospatial Cues on Instructional Static and Dynamic Visualizations on Learner Mental Model Constructions
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Hui-Yu Yang
- Subjects
Human-Computer Interaction ,education ,Information Systems - Abstract
This empirical study investigated whether progressive visuospatial cues presented in a self-regulatory mode could help learners understand the operation of a mechanical system. The learners’ prior knowledge and cueing condition were the independent variables in terms of investigating their effectiveness on retention and transfer test results. A total of 126 English as a foreign language (EFL) learners voluntarily participated in the study. First, their prior knowledge was evaluated. Then, they were assigned to one of the following experimental conditions:—animation-only, entity-cued animation, and arrow-entity-cued animation. Immediately after the experimental treatment, retention and transfer tests as well as a cognitive load questionnaire were administered to assess the learners’ test performance and cognitive load, respectively. The experimental results suggested that progressive snowballing cues were favorable in assisting learners' retention and transfer test results. more...
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- 2022
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3. Effects of Dynamic Visualizations Enriched With Visuospatial Cues on Learners' Cognitive Load and Learning Effectiveness
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Hui-Yu Yang
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General Computer Science ,Education - Abstract
The present study was to explore whether dynamic visualizations enriched with visuospatial cues can optimize learners’ cognitive processing of mechanical systems. The animated conditions and cueing patterns were the independent variables with an attempt to investigate their impacts on retention and transfer tests. Either dynamic or static visualizations with the presence of visuospatial cues served as the instructional material. 238 English as a foreign language (EFL) learners participated in the experiment. The learners were distributed to six groups—either dynamic or static visualizations enriched with non-cue, entity-cues and arrow-entity cues. Their prior knowledge was initially assessed, and then followed by retention and transfer tests and cognitive load measurements. The experimental results suggested that dynamic visualizations enriched with progressive visuospatial cues was more beneficial in helping learners to develop favorable quality of mental models. more...
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- 2022
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4. ATF2 accelerates the invasion and metastasis of hepatocellular carcinoma through targeting the miR‐548p/TUFT1 axis
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Zhen-Jie, Li, Jin-Ping, Zhang, Dong-Ying, Li, Hui-Yu, Yang, and Bing-Rong, Liu
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Infectious Diseases ,Hepatology - Abstract
Due to high invasion and metastasis, hepatocellular carcinoma (HCC) is known as one of the most fatal carcinomas. We aim to further investigate regulatory mechanisms of invasion and metastasis to elucidate HCC pathogenesis and develop novel medications.Patient specimens were collected for assessing gene expression and correlation between gene expressions. The expression of Ki67 and E-cadherin in subcutaneous xenograft tumor were examined by immunohistochemistry staining. The expression of activating transcription factor 2 (ATF2), miR-548p and TUFT1 were determined using Real-time quantitative reverse transcription polymerase chain reaction. Epithelial-mesenchymal transition and PI3K/AKT signaling-associated markers were examined with western blot. The proliferation, migration and invasion were assessed by 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide, colony formation and transwell assays, respectively. Cell apoptosis was assessed via Annexin V and propidium iodide staining. Gene interaction was confirmed using chromatin immunoprecipitation and luciferase activity assays. Subcutaneous and intravenous xenograft mouse models were established for analyzing HCC growth and metastasis in vivo.ATF2 was up-regulated in HCC patients and cells. ATF2 promoted HCC cell proliferation, migration and invasion and inhibited cell apoptosis through directly targeting miR-548p and controlling its expression. miR-548p suppressed HCC cell proliferation, migration and invasion and enhanced cell apoptosis. miR-548p directly bound to the 3'UTR of TUFT1 to restrain its expression and subsequently suppress the PI3K/AKT signaling. ATF2 knock-down significantly suppressed the growth and metastasis of HCC.ATF2 accelerates HCC progression by promoting cell proliferation, migration, invasion and metastasis, which is dependent on regulating the miR-548p/TUFT1 axis. more...
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- 2022
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5. A change of PD-1/PD-L1 expression on peripheral T cell subsets correlates with the different stages of Alzheimer's Disease
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Ching-Tse Wu, Cheng-I Chu, Feng-Yu Wang, Hui-Yu Yang, Wei-Sung Tseng, Chuang-Rung Chang, and Chien-Chung Chang
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General Biochemistry, Genetics and Molecular Biology - Abstract
BackgroundImmune checkpoints are a set of costimulatory and inhibitory molecules that maintain self-tolerance and regulate immune homeostasis. The expression of immune checkpoints on T cells in malignancy, chronic inflammation, and neurodegenerative diseases has gained increasing attention.ResultsTo characterize immune checkpoints in neurodegenerative diseases, we aimed to examine the expression of the immune checkpoint PD-1/PD-L1 in peripheral T cells in different Alzheimer’s disease (AD) patients. To achieve this aim, sixteen AD patients and sixteen age-matched healthy volunteers were enrolled to analyze their CD3+T cells, CD3+CD56+(neural cell adhesion molecule, NCAM) T cells, CD4+/CD8+T cells, and CD4+/CD8+CD25+(interleukin-2 receptor alpha, IL-2RA) T cells in this study. The expression of PD-1 on T cells was similar between the AD patients and healthy volunteers, but increased expression of PD-L1 on CD3+CD56+T cells (natural killer T cells, NKT-like), CD4+T cells (helper T cells, Th), CD4+CD25+T cells, and CD8+T cells (cytotoxic T lymphocytes, CTL) was detected in the AD patients. In addition, we found negative correlations between the AD patients’ cognitive performance and both CD8+T cells and CD8+CD25+T cells. To identify CD8+T-cell phenotypic and functional characteristic differences between the healthy volunteers and AD patients in different stages, a machine learning algorithm, t-distributed stochastic neighbor embedding (t-SNE), was implemented. Using t-SNE enabled the above high-dimensional data to be visualized and better analyzed. The t-SNE analysis demonstrated that the cellular sizes and densities of PD-1/PD-L1 on CD8+T cells differed among the healthy, mild AD, and moderate AD subjects.ConclusionsOur results suggest that changes in PD-1/PD-L1-expressing T cells in AD patients’ peripheral blood could be a potential biomarker for monitoring disease and shed light on the AD disease mechanism. Moreover, these findings indicate that PD-1/PD-L1 blockade treatment could be a novel choice to slow AD disease deterioration. more...
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- 2022
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6. Matriptase and prostasin proteolytic activities are differentially regulated in normal and wounded skin
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Hao Yu Fang, Po Wen A. Du, Chih Hsin Lai, Yu Sin Hung, Yu Hsuan Chen, Hung-Jen Tang, Shun Cheng Chang, Hui Yu Yang, Chien-Ping Chiang, Michael D. Johnson, Jehng-Kang Wang, Chen-Yong Lin, and Shiao Pieng Lee more...
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0301 basic medicine ,Serine protease ,Cancer Research ,Proteases ,Protease ,integumentary system ,biology ,medicine.diagnostic_test ,Chemistry ,medicine.medical_treatment ,Proteolysis ,Human skin ,Cell Biology ,Cell biology ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,030220 oncology & carcinogenesis ,Zymogen ,Zymogen activation ,biology.protein ,medicine ,Matriptase - Abstract
Orchestrated control of multiple overlapping and sequential processes is required for the maintenance of epidermal homeostasis and the response to and recovery from a variety of skin insults. Previous studies indicate that membrane-associated serine protease matriptase and prostasin play essential roles in epidermal development, differentiation, and barrier formation. The control of proteolysis is a highly regulated process, which depends not only on gene expression but also on zymogen activation and the balance between protease and protease inhibitor. Subcellular localization can affect the accessibility of protease inhibitors to proteases and, thus, also represents an integral component of the control of proteolysis. To understand how membrane-associated proteolysis is regulated in human skin, these key aspects of matriptase and prostasin were determined in normal and injured human skin by immunohistochemistry. This staining shows that matriptase is expressed predominantly in the zymogen form at the periphery of basal and spinous keratinocytes, and prostasin appears to be constitutively activated at high levels in polarized organelle-like structures of the granular keratinocytes in the adjacent quiescent skin. The membrane-associated proteolysis appears to be elevated via an increase in matriptase zymogen activation and prostasin protein expression in areas of skin recovering from epidermal insults. There was no noticeable change observed in other regulatory aspects, including the expression and tissue distribution of their cognate inhibitors HAI-1 and HAI-2. This study reveals that the membrane-associated proteolysis may be a critical epidermal mechanism involved in responding to, and recovering from, damage to human skin. more...
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- 2020
- Full Text
- View/download PDF
7. Effects of interactivity and progressive visuospatial cues on learners’ comprehension of dynamic visualizations
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Hui-Yu Yang
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050101 languages & linguistics ,Visual perception ,05 social sciences ,050301 education ,Cognition ,Animation ,Computer Science Applications ,Education ,Comprehension ,Empirical research ,Interactivity ,Transfer of training ,0501 psychology and cognitive sciences ,Psychology ,0503 education ,Cognitive load ,Cognitive psychology - Abstract
The present empirical study investigated whether progressive visuospatial cues presented in a self-regulatory mode could assist learners in comprehending the operation of a mechanical system. In th... more...
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- 2020
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8. Effects of Progressive Visuospatial Cues on EFL Learners’ Processing of Animated Visualizations Through Mobile Device
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Hui-Yu Yang
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Human-Computer Interaction ,Empirical research ,Computer science ,Human–computer interaction ,0502 economics and business ,05 social sciences ,050211 marketing ,0501 psychology and cognitive sciences ,Human Factors and Ergonomics ,Mobile device ,050107 human factors ,Computer Science Applications - Abstract
The present empirical study was to examine whether progressive snowball-like cues can benefit learners in learning the operation of a mechanical system. In particular, this study investigated the e... more...
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- 2019
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9. Highly Stable White Light-Emitting Diodes Based on Quantum-Dots Dispersed Into the Backlight Lens for Display Backlight
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Xu Yan Lan, Yong Zhang, Yu Sheng Liu, Hui Yu Yang, Xing Yang, Shu Wen Zheng, and Yi Li
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lcsh:Applied optics. Photonics ,Materials science ,Quantum-dots (QDs) ,02 engineering and technology ,Backlight ,01 natural sciences ,law.invention ,010309 optics ,on-chip ,Gamut ,display backlight ,law ,0103 physical sciences ,White light ,lcsh:QC350-467 ,Electrical and Electronic Engineering ,in-lens ,Diode ,Liquid-crystal display ,business.industry ,lcsh:TA1501-1820 ,021001 nanoscience & nanotechnology ,Atomic and Molecular Physics, and Optics ,Quantum dot ,Optoelectronics ,white light-emitting diodes (WLEDs) ,0210 nano-technology ,business ,Luminous efficacy ,lcsh:Optics. Light - Abstract
A novel package structure has been developed to fabricate quantum-dot (QD) white light-emitting diodes (WLEDs) by dispersing QDs into the backlight lens and integrating them with blue light-emitting diode to form white light. For this in-lens encapsulation structure, the luminous efficiency and lifetime of QD-WLEDs can reach 90 lm/W and 1000 h, which is 25 times longer than that of QD-WLEDs with on-chip encapsulation structure. Furthermore, a 32-inch prototype liquid crystal display TV shows a wide color gamut of 108% under the National Television Systems Committee standard with this in-lens encapsulation structure. The improvement performance should be attributed to providing a hermetic encapsulation environment preventing oxygen and water from reacting with the QDs and reducing the temperature of QDs due to in-lens package structure. Our results demonstrate that the in-lens solution can be a promising application in display backlight. more...
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- 2019
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10. Cretaceous fire-resistant angiosperms
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Xiao-xuan Long, Xiao-fan Liu, Ri-Xin Jiang, Robert A. Spicer, Xian-Chun Zhang, Zun-tian Zhao, Hui-yu Yang, Natasha Lombard, Qi Feng, Yongdong Wang, Wei-Cai Song, Chun-lin Long, Harald Schneider, Hong-rui Zhang, Jian-ping Zhu, De-Zhu Li, Ji Yuan, Hua Peng, Hao-hong Cai, Chenyang Cai, Qiao-Ping Xiang, Erik Tihelka, Shuo Wang, Xiong Li, Michael S. Engel, and Chao Shi more...
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Paleontology ,Cretaceous ,Geology - Abstract
Background: Flowering plants (angiosperms) dominate most global ecosystems today, but their rapid Cretaceous diversification has remained poorly understood ever since Darwin referred to it as an ‘abominable mystery’. Although numerous Cretaceous fossil flowers have been discovered in recent years, most are represented by incomplete charcoalified fragments that do not preserve delicate structures such as complete petals and surface textures, which means that their similarity to living forms is often difficult to discern. The scarcity of information about the ecology of early angiosperms makes it difficult to test hypotheses about the drivers of their diversification. Among other factors, frequent fires in the Cretaceous have been postulated as having possibly facilitated the rise of angiosperms. However, to date no early fossil angiosperms displaying fire-adapted traits have been known, making the role of fire in shaping Cretaceous floras uncertain.Results: We report the discovery of two exquisitely preserved fossil flower species, one identical to the inflorescences of the extant crown eudicot genus Phylica and the other recovered as a sister group to Phylica, both preserved as inclusions in Cretaceous amber from northern Myanmar (~99 Ma). These specialized flower structures, named Phylica piloburmensis sp. nov. and Eophylica priscastellata gen. et sp. nov., were adapted to surviving frequent wildfires, providing the earliest evidence of fire-resistance in angiosperms. The fossils suggest that fire was a significant selective force in Cretaceous angiosperm floras and that adaptations to fire resistance in some eudicot clades have been conserved for at least 99 Ma. This morphological stasis encompasses a range of floral characters, including the production of ‘pseudo-flowers’, and characteristic fruit and pollen architecture. Given its morphological distinctiveness, the Eophylica-Phylica clade represents one of the first well-documented angiosperm ‘living fossil’ genera from the Cretaceous. Conclusion: Our study suggests that core eudicots with specialised flower morphology displaying hallmarks of fire resistance and identical to those of the extant south African genus Phylica, had originated by the mid-Cretaceous (~99 Ma). Palaeoenvironmental reconstructions indicate that these plants lived in conditions similar to those of present-day southern Africa where 70% of taxa survive frequent burning, and that fire resistance was probably widespread in the fire-prone Cretaceous. The results also provide new insights into the biogeographic origin of at least one element of the highly endemic Greater Cape Region biodiversity hotspot flora biota. more...
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- 2021
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11. Fire-prone Rhamnaceae with South African affinities in Cretaceous Myanmar amber
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Chao Shi, Shuo Wang, Hao-hong Cai, Hong-rui Zhang, Xiao-xuan Long, Erik Tihelka, Wei-cai Song, Qi Feng, Ri-xin Jiang, Chen-yang Cai, Natasha Lombard, Xiong Li, Ji Yuan, Jian-ping Zhu, Hui-yu Yang, Xiao-fan Liu, Qiao-ping Xiang, Zun-tian Zhao, Chun-lin Long, Harald Schneider, Xian-chun Zhang, Hua Peng, De-Zhu Li, Yong Fan, Michael S. Engel, Yong-dong Wang, and Robert A. Spicer more...
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Fossils ,Rhamnaceae ,Plant Science ,Myanmar ,Ecosystem ,Amber - Abstract
The rapid Cretaceous diversification of flowering plants remains Darwin's 'abominable mystery' despite numerous fossil flowers discovered in recent years. Wildfires were frequent in the Cretaceous and many such early flower fossils are represented by charcoalified fragments, lacking complete delicate structures and surface textures, making their similarity to living forms difficult to discern. Furthermore, scarcity of information about the ecology of early angiosperms makes it difficult to test hypotheses about the drivers of their diversification, including the role of fire in shaping flowering plant evolution. We report the discovery of two exquisitely preserved fossil flower species, one identical to the inflorescences of the extant crown-eudicot genus Phylica and the other recovered as a sister group to Phylica, both preserved as inclusions together with burned plant remains in Cretaceous amber from northern Myanmar (~99 million years ago). These specialized flower species, named Phylica piloburmensis sp. nov. and Eophylica priscastellata gen. et sp. nov., exhibit traits identical to those of modern taxa in fire-prone ecosystems such as the fynbos of South Africa, and provide evidence of fire adaptation in angiosperms. more...
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- 2021
12. An Integrated Response-Surface-Based Method for Simulation Optimization with Correlated Outputs
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Kuo-Hao Chang, Robert Cuckler, and Hui-Yu Yang
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Surface (mathematics) ,Simulation optimization ,Computer science ,Ocean Engineering ,Point (geometry) ,Response surface methodology ,Management Science and Operations Research ,Algorithm - Abstract
While nearly all previous algorithms designed to solve simulation optimization problems have treated the outputs of simulation systems at a given design point (input parameter) as being independent of each other, this premise is flawed in that simulated outputs are generally correlated. We propose a decorrelation (DC) procedure that can effectively evaluate and remove the correlation of outputs of a simulation system. The proposed DC procedure is further integrated with STRONG, an improved framework of the well-known Response Surface Methodology (RSM), for tackling the simulation optimization problems with correlated outputs. This integration is particularly synergistic due to the fact that STRONG is a fully automated, response-surface-based procedure possessing appealing convergence properties and DC can take advantage of the concept of trust region as in STRONG to enable the removal of the correlation of outputs at the design points within the same trust region all at once. This is more efficient compared to the traditional approaches where a substantial number of observations are typically required for dealing with correlations. The resulting integrated method, which we call STRONG-DC, requires various adaptations so as to ensure the efficacy and efficiency of the overall framework. STRONG-DC preserves the desirable automation and convergence as STRONG, namely, it does not require human involvements and can be proved to achieve the truly optimal solution(s) with probability one (w.p.1) under reasonable conditions. Moreover, the effectiveness and efficiency of STRONG-DC are evaluated through extensive numerical analyses, along with a case study involving the well-known newsvendor problem. more...
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- 2021
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13. Alcoholic fatty liver disease inhibited the co-expression of Fmo5 and PPARα to activate the NF-κB signaling pathway, thereby reducing liver injury via inducing gut microbiota disturbance
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Ling jian Kong, Jing Chen, Xiao li Ji, Qian Qin, Hui yu Yang, Dan Liu, De liang Li, and Mei ling Sun
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BackgroundAlcohol-induced intestinal dysbiosis disrupts and inflammatory responses are essential in the development of alcoholic fatty liver disease (AFLD). Here, we investigated the effects of Fmo5 on changes in enteric microbiome composition in a model of AFLD and dissected the pathogenic role of Fmo5 in AFLD-induced liver pathology.MethodsThe expression profile data of GSE8006 and GSE40334 datasets were downloaded from the GEO database. The WGCNA approach allowed us to investigate the AFLD-correlated module. DEGs were used to perform KEGG pathway enrichment analyses. Four PPI networks were constructed using the STRING database and visualized using Cytoscape software. The Cytohubba plug-in was used to identify the hub genes. Western blot and immunohistochemistry assays were used to detect protein expression. ELISA assay was used to detect the levels of serum inflammatory cytokines. Lipid droplets in the cytoplasm were observed using Oil Red O staining. Apoptosis was detected using a TUNEL assay and flow cytometry analysis. ROS levels were detected using flow cytometry analysis. Nuclear translocation of NF-κB p65 was observed using immunofluorescence staining. Co-immunoprecipitation was used to detect the co-expression of PPARα and Fmo5 in L02 cells. 16S rDNA sequencing defined the bacterial communities in mice with AFLD.ResultsFmo5 is a key DEG and is closely associated with the gut microbiota and PPAR signaling pathway. Gut microbiome function in AFLD was significantly related to the PPAR signaling pathway. AFLD induced shifts in various bacterial phyla in the cecum, including a reduction in Bacteroidetes and increased Firmicutes. Fmo5 and PPARα co-expression in cell and animal models with AFLD, which decreased significantly. Silencing of Fmo5 and PPARα aggravated the functions of AFLD inducing apoptosis and inflammatory response, promoting liver injury, and activating the NF-κB signaling pathway in vivo and in vitro. The NF-κB inhibitor abolished the functions of silencing of Fmo5 and PPARα promoting AFLD-induced apoptosis, inflammatory response, and liver injury.ConclusionOur data indicated that the co-expression of Fmo5 and PPARα was involved in AFLD-related gut microbiota composition and alleviated AFLD-induced liver injury, apoptosis, and inflammatory response by inhibiting the nuclear translocation of NF-κB p65 to inhibit the NF-κB signaling pathway. more...
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- 2020
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14. Novel transcription regulatory sequences and factors of the immune evasion protein ICP47 (US12) of herpes simplex viruses
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Lin-Zhong Zhu, Xian-Wang Wang, Hui-Yu Yang, Wen-Qi Cai, Ying Xiang, Zhaowu Ma, Yang Zhou, Jun-Ting Cheng, Zi-Wen Han, Bing-Rong Liu, Ying Zhang, Xiao-Chun Peng, Hong-Wu Xin, Shuzhong Cui, and Ying-Ying Wang more...
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0301 basic medicine ,Gene Expression Regulation, Viral ,Male ,China ,ICP47 ,Transcription, Genetic ,viruses ,Transcriptional regulation factor (TRF) ,Herpesvirus 1, Human ,Biology ,medicine.disease_cause ,Virus Replication ,lcsh:Infectious and parasitic diseases ,Immediate-Early Proteins ,03 medical and health sciences ,0302 clinical medicine ,Transcription (biology) ,Virology ,Transcriptional regulation ,medicine ,Humans ,lcsh:RC109-216 ,Gene ,Transcription factor ,Phylogeny ,Immune Evasion ,Research ,Herpes Simplex ,HSV-2 ,HSV-1 ,Transcriptional regulation sequence (TRS) ,Oncolytic virus ,US12 ,030104 developmental biology ,Infectious Diseases ,Herpes simplex virus ,Viral replication ,Regulatory sequence ,030220 oncology & carcinogenesis - Abstract
Background Herpes simplex virus (HSV) can cause encephalitis. Its infected cell polypeptide 47 (ICP47), encoded by immediate-early gene US12, promotes immune escape. ICP47 was modified in the clinically approved oncolytic HSV (oHSV) T-Vec. However, transcription regulatory sequence (TRS) and transcription regulatory factor (TRF) of HSV US12 are seldom reported. Methods Previously, our laboratory isolated a new HSV strain named HSV-1-LXMW from a male patient with oral herpes in Beijing, China. Firstly, the genetic tree was used to analyze its genetic relationship. The US12 TRS and TRF in HSV-1-LXMW were found by using predictive software. Secondly, the further verification by the multi-sequence comparative analysis shown that the upstream DNA sequence of HSV US12 gene contained the conserved region. Finally, the results of literature search shown that the expression of transcription factors was related to the tissue affinity of HSV-1 and HSV-2, so as to increase the new understanding of the transcriptional regulation of HSV biology and oncolytic virus (OVs) therapy. Results Here we reported the transcriptional regulation region sequence of our new HSV-1-LXMW, and its close relationship with HSV-1-CR38 and HSV-1-17. Importantly we identified eight different kinds of novel TRSs and TRFs of HSV US12 for the first time, and found they are conserved among HSV-1 (c-Rel, Elk-1, Pax-4), HSV-2 (Oct-1, CF2-II, E74A, StuAp) or both HSVs (HNF-4). The TRFs c-Rel and Oct-1 are biologically functional respectively in immune escape and viral replication during HSV infection. Conclusions Our findings have important implication to HSV biology, infection, immunity and oHSVs. more...
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- 2020
15. Matriptase and prostasin proteolytic activities are differentially regulated in normal and wounded skin
- Author
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Shun-Cheng, Chang, Chien-Ping, Chiang, Chih-Hsin, Lai, Po-Wen A, Du, Yu-Sin, Hung, Yu-Hsuan, Chen, Hui-Yu, Yang, Hao-Yu, Fang, Shiao-Pieng, Lee, Hung-Jen, Tang, Jehng-Kang, Wang, Michael D, Johnson, and Chen-Yong, Lin more...
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Enzyme Precursors ,Wound Healing ,Membrane Glycoproteins ,Skin Physiological Phenomena ,Proteolysis ,Serine Endopeptidases ,Proteinase Inhibitory Proteins, Secretory ,Gene Expression ,Gene Expression Regulation, Developmental ,Humans ,Wounds and Injuries ,Cells, Cultured ,Skin - Abstract
Orchestrated control of multiple overlapping and sequential processes is required for the maintenance of epidermal homeostasis and the response to and recovery from a variety of skin insults. Previous studies indicate that membrane-associated serine protease matriptase and prostasin play essential roles in epidermal development, differentiation, and barrier formation. The control of proteolysis is a highly regulated process, which depends not only on gene expression but also on zymogen activation and the balance between protease and protease inhibitor. Subcellular localization can affect the accessibility of protease inhibitors to proteases and, thus, also represents an integral component of the control of proteolysis. To understand how membrane-associated proteolysis is regulated in human skin, these key aspects of matriptase and prostasin were determined in normal and injured human skin by immunohistochemistry. This staining shows that matriptase is expressed predominantly in the zymogen form at the periphery of basal and spinous keratinocytes, and prostasin appears to be constitutively activated at high levels in polarized organelle-like structures of the granular keratinocytes in the adjacent quiescent skin. The membrane-associated proteolysis appears to be elevated via an increase in matriptase zymogen activation and prostasin protein expression in areas of skin recovering from epidermal insults. There was no noticeable change observed in other regulatory aspects, including the expression and tissue distribution of their cognate inhibitors HAI-1 and HAI-2. This study reveals that the membrane-associated proteolysis may be a critical epidermal mechanism involved in responding to, and recovering from, damage to human skin. more...
- Published
- 2020
16. Random RotBoost: An Ensemble Classification Method Based on Rotation Forest and AdaBoost in Random Subsets and Its Application to Clinical Decision Support
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Shin-Jye Lee, Ching-Hsun Tseng, Hui-Yu Yang, Xin Jin, Qian Jiang, Bin Pu, Wei-Huan Hu, Duen-Ren Liu, Yang Huang, and Na Zhao
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classification ,Rotation Forest ,AdaBoost ,clinical decision support ,General Physics and Astronomy - Abstract
In the era of bathing in big data, it is common to see enormous amounts of data generated daily. As for the medical industry, not only could we collect a large amount of data, but also see each data set with a great number of features. When the number of features is ramping up, a common dilemma is adding computational cost during inferring. To address this concern, the data rotational method by PCA in tree-based methods shows a path. This work tries to enhance this path by proposing an ensemble classification method with an AdaBoost mechanism in random, automatically generating rotation subsets termed Random RotBoost. The random rotation process has replaced the manual pre-defined number of subset features (free pre-defined process). Therefore, with the ensemble of the multiple AdaBoost-based classifier, overfitting problems can be avoided, thus reinforcing the robustness. In our experiments with real-world medical data sets, Random RotBoost reaches better classification performance when compared with existing methods. Thus, with the help from our proposed method, the quality of clinical decisions can potentially be enhanced and supported in medical tasks. more...
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- 2022
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17. The effects of visual cueing on pictorial and verbal tests through mobile-phone-based animation
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Hui-Yu Yang
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Matching (statistics) ,Variables ,media_common.quotation_subject ,Educational technology ,Animation ,Computer Science Applications ,Education ,Test (assessment) ,Comprehension ,Selection (linguistics) ,Psychology ,Cognitive load ,Cognitive psychology ,media_common - Abstract
The purpose of this quasi-experimental study was to examine whether attention cueing can assist learners in learning the structure and functions of the brain. This study investigated the interactive effects of the experimental treatment and learners’ prior knowledge on their test results and cognitive load. Self-regulatory, mobile-phone-based animations depicting the brain comprised the instrument. A total of 55 English as a foreign language learners from two sections of a linguistics course were recruited. First, the participants’ prior knowledge concerning the functions of the brain was assessed. Next, they were randomly assigned into two modes—animation-only or animation-plus-cueing. Immediately, after the treatment period, the participants were administered retention, matching, organization, and transfer test along with cognitive load questionnaire. Experimental treatment and prior knowledge were the independent variables, while comprehension tests, cognitive load, and study time were the dependent variables. The results demonstrated that the learners in the cueing condition outperformed the non-cueing condition for matching and organization test, but not for transfer test. No differences were observed between the cueing and non-cueing condition concerning the cognitive load. The results stressed the importance of presenting relation cueing emphasizing cause–effect relationship on dynamic external representations can facilitate learners’ selection and organization of information so as to enhance general comprehension of external representations. more...
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- 2018
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18. Discovery of the first Onthophilus species from mid-Cretaceous Burmese amber (Coleoptera: Histeridae)
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Chao Shi, Ri-Xin Jiang, Wei-Cai Song, Shuo Wang, and Hui-yu Yang
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010506 paleontology ,biology ,Paleontology ,Zoology ,010502 geochemistry & geophysics ,biology.organism_classification ,01 natural sciences ,Cretaceous ,language.human_language ,Burmese ,Geography ,Extant taxon ,Genus ,language ,Histeridae ,Onthophilus ,0105 earth and related environmental sciences - Abstract
For the first time, the histerid genus Onthophilus Leach, 1817 is found from the mid-Cretaceous Burmese amber with a new species Onthophilus yingae sp. nov. The new species can be clearly assigned to the genus Onthophilus by sharing an identical set of characters with the extant species. As direct evidence, this new finding demonstrates the ancient origin of Onthophilus. more...
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- 2020
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19. Pure NOTES Trans-Rectal Gallbladder Preserving Cholecystolithotomy: The First Reported Clinical Series
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Bing-Rong Liu, Li-Xia Zhao, Saif Ullah, Dan Liu, Ling-Jian Kong, Bing Du, Zhong-Hong Liu, Shi-Zhu Jin, De-Liang Li, Yang-Yang Zhou, Cheng-Qian Lv, Ji-Tao Song, Hui-Yu Yang, Si-Ran Zhou, and Ji-Yu Zhang more...
- Published
- 2019
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20. Crystallization mechanism and photocatalytic performance of vanadium-modified bismuth oxide through precipitation processes at room temperature
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Yi Ting Huang, Yu Chun Wu, and Hui Yu Yang
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Materials science ,Precipitation (chemistry) ,Inorganic chemistry ,Doping ,Oxide ,Vanadium ,chemistry.chemical_element ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,01 natural sciences ,0104 chemical sciences ,law.invention ,Bismuth ,chemistry.chemical_compound ,chemistry ,law ,Phase (matter) ,Photocatalysis ,General Materials Science ,Crystallization ,0210 nano-technology - Abstract
Well-crystallized bismuth oxides (Bi2O3) were synthesized through precipitation processes operating at room temperature. Two synthesis routes were used, which led to Bi2O3 products with completely different phase structures. The crystallization mechanisms of γ-Bi2O3 and δ-Bi2O3 in relation to the synthetic processes were discussed. The role of vanadium (V) ions and the doping limit on the structural modification were also investigated. The photocatalytic efficiencies of the V-modified γ-Bi2O3 and δ-Bi2O3 compared with TiO2 P25 powder were discussed. more...
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- 2016
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21. Angiotensin-(1-7) attenuates hypertension and cardiac hypertrophy
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Bin, Liang, Ya-Nan, Zhao, Xin, Wang, Xiao-Jing, Yu, Ying, Li, Hui-Yu, Yang, Qing, Su, Yu-Ming, Kang, and Zhi-Ming, Yang
- Abstract
Angiotensin-(1-7) [Ang-(1-7)] is a multifunctional bioactive angiotensin peptide which exerts a cardiovascular protective function mainly by opposing the effects of angiotensin II. We aimed to determine whether brain Ang-(1-7) regulates nitric oxide (NO) and neurotransmitter levels in the hypothalamic paraventricular nucleus (PVN), and influences sympathetic activity, blood pressure and cardiac hypertrophy in salt-sensitive hypertension. Dahl salt-sensitive rats receiving a high-salt (HS, 8% NaCl) or a normal-salt (NS, 0.3% NaCl) diet were treated with an intracerebroventricular (ICV) infusion of Ang-(1-7) for 6 weeks. Seven rats were measured in each group. In comparison with NS rats, HS rats exhibited significantly increased mean arterial pressure, plasma norepinephrine (NE) and cardiac hypertrophy. In addition, HS rats (compared to NS rats) had increased glutamate, NE and tyrosine hydroxylase (TH) expression, and reduced NO levels as well as reduced expression of γ-aminobutyric acid (GABA) and the 67 kDa isoform of glutamate decarboxylase (GAD67) in the PVN. Treatment with ICV infusion of Ang-(1-7) reversed these changes in the salt-sensitive hypertensive rats. The results suggest that the beneficial effects of brain Ang-(1-7) on salt-sensitive hypertension and cardiac hypertrophy are partly due to an elevation in the NO level and restoration of neurotransmitter balance in the PVN. more...
- Published
- 2017
22. Hemodynamic, Autonomic, and Vascular Effects of Exposure to Coarse Particulate Matter Air Pollution from a Rural Location
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Robert D. Brook, Mariana J. Kaplan, Sanjay Rajagopalan, Bhramar Mukherjee, Nevin Ajluni, Jack R. Harkema, Jeffrey R. Brook, Lu Wang, Elif A. Oral, Robert L. Bard, J. Timothy Dvonch, Srilakshmi Yalavarthi, Catherine Spino, Masako Morishita, Hui Yu Yang, and Qinghua Sun more...
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Adult ,Male ,Rural Population ,Adolescent ,Fine particulate ,Health, Toxicology and Mutagenesis ,Air pollution ,Hemodynamics ,Blood Pressure ,medicine.disease_cause ,Cardiovascular Physiological Phenomena ,Young Adult ,Double-Blind Method ,Heart Rate ,Adverse health effect ,Air Pollution ,medicine ,Humans ,Particle Size ,Inhalation exposure ,Air Pollutants ,Inhalation Exposure ,Cross-Over Studies ,Research ,Public Health, Environmental and Occupational Health ,Rural location ,Middle Aged ,Particulates ,3. Good health ,Blood pressure ,13. Climate action ,Environmental chemistry ,Environmental science ,Female ,Particulate Matter - Abstract
Background: Fine particulate matter (PM) air pollution is associated with numerous adverse health effects, including increased blood pressure (BP) and vascular dysfunction. Coarse PM substantially contributes to global air pollution, yet differs in characteristics from fine particles and is currently not regulated. However, the cardiovascular (CV) impacts of coarse PM exposure remain largely unknown. Objectives: Our goal was to elucidate whether coarse PM, like fine PM, is itself capable of eliciting adverse CV responses. Methods: We performed a randomized double-blind crossover study in which 32 healthy adults (25.9 ± 6.6 years of age) were exposed to concentrated ambient coarse particles (CAP; 76.2 ± 51.5 μg/m3) in a rural location and filtered air (FA) for 2 hr. We measured CV outcomes during, immediately after, and 2 hr postexposures. Results: Both systolic (mean difference = 0.32 mmHg; 95% CI: 0.05, 0.58; p = 0.021) and diastolic BP (0.27 mmHg; 95% CI: 0.003, 0.53; p = 0.05) linearly increased per 10 min of exposure during the inhalation of coarse CAP when compared with changes during FA exposure. Heart rate was on average higher (4.1 bpm; 95% CI: 3.06, 5.12; p < 0.0001) and the ratio of low-to-high frequency heart rate variability increased (0.24; 95% CI: 0.07, 0.41; p = 0.007) during coarse particle versus FA exposure. Other outcomes (brachial flow-mediated dilatation, microvascular reactive hyperemia index, aortic hemodynamics, pulse wave velocity) were not differentially altered by the exposures. Conclusions: Inhalation of coarse PM from a rural location is associated with a rapid elevation in BP and heart rate during exposure, likely due to the triggering of autonomic imbalance. These findings add mechanistic evidence supporting the biological plausibility that coarse particles could contribute to the triggering of acute CV events. Citation: Brook RD, Bard RL, Morishita M, Dvonch JT, Wang L, Yang HY, Spino C, Mukherjee B, Kaplan MJ, Yalavarthi S, Oral EA, Ajluni N, Sun Q, Brook JR, Harkema J, Rajagopalan S. 2014. Hemodynamic, autonomic, and vascular effects of exposure to coarse particulate matter air pollution from a rural location. Environ Health Perspect 122:624–630; http://dx.doi.org/10.1289/ehp.1306595 more...
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- 2014
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23. The effects of visuospatial cueing on EFL learners' science text and picture processing through mobile phones
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Hui-Yu Yang
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Cued speech ,Sociology and Political Science ,020209 energy ,media_common.quotation_subject ,education ,05 social sciences ,Human Factors and Ergonomics ,02 engineering and technology ,Animation ,Troubleshooting ,Education ,Test (assessment) ,Comprehension ,Reading (process) ,0502 economics and business ,Picture processing ,0202 electrical engineering, electronic engineering, information engineering ,Business and International Management ,Psychology ,050203 business & management ,Cognitive load ,Cognitive psychology ,media_common - Abstract
This quasi-experimental study examined whether or not attention cueing can benefit learners in learning the kinematic operation of a mechanical system. This study investigated the interaction effects of the dynamic and cued conditions on the test results as well as cognitive load. Self-regulatory, mobile-phone-based visualizations depicting the kinematics of a flushing cistern comprised the instrument. The participants consisted of 174 English as a foreign language (EFL) learners from four sections of a reading course. After assessing their backgrounds, they were randomly assigned to one of four experimental conditions—pictures only, pictures plus cueing, animation only, and animation plus cueing. Immediately, after administering the experimental treatment, the participants answered comprehension tests and a cognitive load questionnaire. The results demonstrated that visuospatial cues, particularly in static diagrams, ameliorated the learners' learning effectiveness by facilitating their local comprehension better than their global comprehension. While animation benefited the learners in terms of solving directions and troubleshooting problems, the static diagrams and animations complemented each other in helping the learners understand the different kinematic functions of a mechanical system. The static diagrams were beneficial in enhancing local comprehension, whereas the animations benefited the learners' global comprehension as indicated in the learners' transfer test results. The experimental results revealed that the effectiveness of animation and visual cueing depend on the subject matter and purpose of the tests. more...
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- 2019
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24. Cardiovascular Depression in Rats Exposed to Inhaled Particulate Matter and Ozone: Effects of Diet-Induced Metabolic Syndrome
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Catherine Spino, Robert D. Brook, Gregory D. Fink, Bhramar Mukherjee, Sanjay Rajagopalan, Hui Yu Yang, Bin Nan, Jack R. Harkema, Masako Morishita, Qinghua Sun, J. Timothy Dvonch, James G. Wagner, and Katryn Allen more...
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Male ,medicine.medical_specialty ,Ozone ,Fine particulate ,Health, Toxicology and Mutagenesis ,Blood Pressure ,complex mixtures ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,Heart Rate ,Internal medicine ,Heart rate ,Animals ,Medicine ,Depression (differential diagnoses) ,Metabolic Syndrome ,business.industry ,Research ,Public Health, Environmental and Occupational Health ,Particulates ,medicine.disease ,Rats ,Sprague dawley ,Blood pressure ,Endocrinology ,chemistry ,Cardiovascular Diseases ,Particulate Matter ,Metabolic syndrome ,business ,Environmental Monitoring - Abstract
Background: High ambient levels of ozone (O3) and fine particulate matter (PM2.5) are associated with cardiovascular morbidity and mortality, especially in people with preexisting cardiopulmonary diseases. Enhanced susceptibility to the toxicity of air pollutants may include individuals with metabolic syndrome (MetS). Objective: We tested the hypothesis that cardiovascular responses to O3 and PM2.5 will be enhanced in rats with diet-induced MetS. Methods: Male Sprague-Dawley rats were fed a high-fructose diet (HFrD) to induce MetS and then exposed to O3, concentrated ambient PM2.5, or the combination of O3 plus PM2.5 for 9 days. Data related to heart rate (HR), HR variability (HRV), and blood pressure (BP) were collected. Results: Consistent with MetS, HFrD rats were hypertensive and insulin resistant, and had elevated fasting levels of blood glucose and triglycerides. Decreases in HR and BP, which were found in all exposure groups, were greater and more persistent in HFrD rats compared with those fed a normal diet (ND). Coexposure to O3 plus PM2.5 induced acute drops in HR and BP in all rats, but only ND rats adapted after 2 days. HFrD rats had little exposure-related changes in HRV, whereas ND rats had increased HRV during O3 exposure, modest decreases with PM2.5, and dramatic decreases during O3 plus PM2.5 coexposures. Conclusions: Cardiovascular depression in O3- and PM2.5-exposed rats was enhanced and prolonged in rats with HFrD-induced MetS. These results in rodents suggest that people with MetS may be prone to similar exaggerated BP and HR responses to inhaled air pollutants. Citation: Wagner JG, Allen K, Yang HY, Nan B, Morishita M, Mukherjee B, Dvonch JT, Spino C, Fink GD, Rajagopalan S, Sun Q, Brook RD, Harkema JR. 2014. Cardiovascular depression in rats exposed to inhaled particulate matter and ozone: effects of diet-induced metabolic syndrome. Environ Health Perspect 122:27–33; http://dx.doi.org/10.1289/ehp.1307085 more...
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- 2014
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25. No effect of acute exposure to coarse particulate matter air pollution in a rural location on high-density lipoprotein function
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Sanjay Rajagopalan, J. Timothy Dvonch, Catherine Spino, Alma Barajas-Espinosa, Robert D. Brook, Lu Wang, Bhramar Mukherjee, Fen Yin, Andrei Maiseyeu, Jack R. Harkema, Masako Morishita, Jesus A. Araujo, Gajalakshmi Ramanathan, Robert L. Bard, Marcus A. Badgeley, Hui Yu Yang, and Qinghua Sun more...
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Adult ,Male ,Rural Population ,medicine.medical_specialty ,Adolescent ,Health, Toxicology and Mutagenesis ,Air pollution ,Toxicology ,medicine.disease_cause ,Article ,Mice ,Young Adult ,chemistry.chemical_compound ,High-density lipoprotein ,Double-Blind Method ,Air Pollution ,Cell Line, Tumor ,Internal medicine ,medicine ,Animals ,Humans ,Particle Size ,Air Pollutants ,Cross-Over Studies ,Aryldialkylphosphatase ,Chemistry ,Cholesterol ,Macrophages ,Venous blood ,Middle Aged ,Particulates ,Crossover study ,Endocrinology ,Quartile ,Immunology ,Female ,Particulate Matter ,lipids (amino acids, peptides, and proteins) ,Lipoproteins, HDL ,Lipoprotein - Abstract
High-density lipoprotein (HDL) particles perform numerous vascular-protective functions. Animal studies demonstrate that exposure to fine or ultrafine particulate matter (PM) can promote HDL dysfunction. However, the impact of PM on humans remains unknown.We aimed to determine the effect of exposure to coarse concentrated ambient particles (CAP) on several metrics of HDL function in healthy humans.Thirty-two adults (25.9 ± 6.6 years) were exposed to coarse CAP [76.2 ± 51.5 µg·m(-3)] in a rural location and filtered air (FA) for 2 h in a randomized double-blind crossover study. Venous blood collected 2- and 20-h post-exposures was measured for HDL-mediated efflux of [(3)H]-cholesterol from cells and 20-h exposures for HDL anti-oxidant capacity by a fluorescent assay and paraoxonase activity. The changes [median (first, third quartiles)] between exposures among 29 subjects with available results were compared by matched Wilcoxon tests.HDL-mediated cholesterol efflux capacity did not differ between exposures at either time point [16.60% (15.17, 19.19) 2-h post-CAP versus 17.56% (13.43, 20.98) post-FA, p = 0.768 and 14.90% (12.47, 19.15) 20-h post-CAP versus 17.75% (13.22, 23.95) post-FA, p = 0.216]. HOI [0.26 (0.24, 0.35) versus 0.28 (0.25, 0.40), p = 0.198] and paraoxonase activity [0.54 (0.39, 0.82) versus 0.60 μmol·min(-1 )ml plasma(-1) (0.40, 0.85), p = 0.137] did not differ 20-h post-CAP versus FA, respectively.Brief inhalation of coarse PM from a rural location did not acutely impair several facets of HDL functionality. Whether coarse PM derived from urban sites, fine particles or longer term PM exposures can promote HDL dysfunction warrant future investigations. more...
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- 2014
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26. Application of Fault Diagnosis for Air Blower Based on Genetic Fuzzy Neural Network
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Hui Yu Yang and Xiao Hui Li
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Engineering ,Local optimum ,Artificial neural network ,business.industry ,General Medicine ,Artificial intelligence ,Fault (power engineering) ,business ,Fuzzy logic ,Air blower - Abstract
The way of fault characteristic parameters fuzzy processing and optimizing the weights and thresholds of ANN by GA are studied. As a result, the convergent rate and convergent precision are greatly increased. Application to the fault diagnosis of a air blower system shows the new model overcomes the low learning rate and local optima of BP algorithm, and the fault diagnosis precision is effectively improved. more...
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- 2013
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27. Extracts of Koelreuteria henryi Dummer induce apoptosis and autophagy by inhibiting dihydrodiol dehydrogenase, thus enhancing anticancer effects
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Hui Yu Yang, Kuan-Chih Chow, Cheng Lin Yang, Shiow-Her Chiou, Janaki N. Sudhakar, Shu-Fen Chiang, Shu Li Wang, Ching Kuo Lee, Hsiu Ching Yang, Chih Yi Chen, Chien Min Chen, Yung Yen Chiang, Shwn-Huey Shieh, Hsin Yuan Fang, and Hsiu Wen Huang more...
- Subjects
DNA repair ,Antineoplastic Agents ,Apoptosis ,Biology ,Ceramides ,Sapindaceae ,Downregulation and upregulation ,Cell Line, Tumor ,Autophagy ,Genetics ,Humans ,Cytotoxic T cell ,Gene silencing ,Enzyme Inhibitors ,Kaempferols ,chemistry.chemical_classification ,Plant Extracts ,Cell Membrane ,Drug Synergism ,General Medicine ,Enzyme ,chemistry ,Cancer cell ,Cancer research ,Oxidoreductases - Abstract
Dihydrodiol dehydrogenase (DDH) is frequently detected in cancer cells, and its overexpression correlates with drug resistance, the downregulation of DNA repair mechanisms, increased frequency of tumor recurrence, cancer cell metastasis and poor prognosis. The silencing of DDH expression using siRNA, on the other hand, reduces drug resistance and cancer cell mobility. These data suggest that DDH may be an oncogene-related protein. However, no specific DDH inhibitor has been identified to date. Thus, in this study, we used DDH as a target enzyme in a live-cell enzyme-linked immunosorbent assay to screen Chinese medicinal herb extracts (CMHEs) with the aim of identifying a DDH inhibitor. Using this method, we found 49 among 796 CMHEs that inhibited DDH expression. We selected three potential extracts, which had the highest activity against DDH, for further fractionation using high-performance liquid chromatography. The active ingredient was identified by immunoblot analysis. The function of the active ingredient was characterized by cell function analysis. Our results revealed that the CMHE-purified compounds targeted DDH, inducing autophagy and reducing DNA repair, which in turn enhanced the cytotoxic effects of the anticancer drugs and irradiation. more...
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- 2013
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28. Morphology-controllable Bi2O3 crystals through an aqueous precipitation method and their photocatalytic performance
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Sea-Fue Wang, Hui Yu Yang, Yu Chun Wu, Yu Chen Chaing, and Chi Yuen Huang
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Aqueous solution ,Materials science ,Process Chemistry and Technology ,General Chemical Engineering ,Oxide ,Analytical chemistry ,chemistry.chemical_element ,Crystal growth ,Microstructure ,Bismuth ,Crystal ,chemistry.chemical_compound ,Crystallography ,chemistry ,Photocatalysis ,Titration - Abstract
This study investigated the formation of bismuth oxide (Bi 2 O 3 ) crystals by an aqueous precipitation method using bismuth nitrate pentahydrate [Bi(NO 3 ) 3 ·5H 2 O] and NaOH. The phase evolution during NaOH titration was also studied. Basic bismuth nitrates were formed during NaOH titration. However, these nitrates dissolved and recrystallized into Bi 2 O 3 when the pH attained a critical range between 13 and 14. A second phase of Bi(OH) 3 was induced when the pH of the solution was over the critical pH range. The morphology of the Bi 2 O 3 crystal strongly depended on precipitation conditions. The NO 3 − ions favored the crystal growth along the [001] direction to obtain needle-like Bi 2 O 3 crystals. Conversely, NaOH tended to be absorbed on the (001) face and suppressed the preferred growth in the [001] direction because of the shielding effect. The crystal changed from needle-like to plate or polyhedral shape with increasing NaOH concentration. The optical absorption properties were also modified accordingly, resulting in the change of color from yellow to pale orange because of size and shape effects. Finally, Bi 2 O 3 crystals with plate and polyhedral morphologies were found to show relatively higher photocatalytic activity than the needle-like crystals due to the better development of the active crystal faces with suppression of the preferred crystal growth habit. more...
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- 2013
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29. Manufacturing Technique and Property Evaluation of Cotton/Polyester/ Rubber Composite Warp Knit
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Ching Wen Lou, Jin Mao Chen, Shih Yu Huang, Hui Yu Yang, Jia-Horng Lin, and Ching Wen Lin
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Materials science ,Textile ,business.industry ,Warp knitting ,General Engineering ,Environmental pollution ,Yarn ,Structural engineering ,Synthetic fiber ,Natural rubber ,visual_art ,Air permeability specific surface ,Ultimate tensile strength ,visual_art.visual_art_medium ,Composite material ,business - Abstract
Cotton fiber is a type of natural fibers. Using natural fibers to fabricate textile can not only decrease the consumption of synthetic fibers, but also reduce the environmental pollution. This study aims to fabricate elastic knitted fabrics and evaluate their properties. Polyester (PET) filaments and rubber threads serve as the warp while cotton yarn serves as the weft for warp knitting. A crochet machine makes the warp and weft into warp knits with desirable stretchability, during which the amount (single/double) and the ply number (1-, 2-, and 3-ply) of the weft are further varied. The resulting warp knits are evaluated for water absorption, air permeability, and mechanical properties. As demonstrated by the experimental results, the warp knits with single 1-ply weft (S1) yield an optimal air permeability of 224.6 cm3/cm2/s and stiffness along the warp direction of 4.74cm. The warp knits with single 2-ply weft (S2) display an optimal tearing strength of 86N while the warp knits with double 3-ply weft (D3/3) has an optimal tensile strength of 708N. more...
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- 2012
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30. Utility of oral nicardipine and magnesium sulfate infusion during preparation and resection of pheochromocytomas
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Gerard M. Doherty, Hasan K. Siddiqi, Paul G. Gauger, Amanda M. Laird, Amy C. Fox, Barbra S. Miller, and Hui Yu Yang
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Adult ,Male ,Phenoxybenzamine ,Nicardipine ,Population ,Adrenal Gland Neoplasms ,Administration, Oral ,Hemodynamics ,Blood Pressure ,Pheochromocytoma ,Normetanephrine ,Preoperative care ,Magnesium Sulfate ,Young Adult ,chemistry.chemical_compound ,Preoperative Care ,medicine ,Humans ,Infusions, Intravenous ,education ,Adrenergic alpha-Antagonists ,Antihypertensive Agents ,Aged ,education.field_of_study ,Intraoperative Care ,business.industry ,Middle Aged ,Calcium Channel Blockers ,equipment and supplies ,medicine.disease ,Blood pressure ,chemistry ,Anesthesia ,Female ,Surgery ,business ,medicine.drug - Abstract
Calcium channel blockade with nicardipine (NC) is an alternative to phenoxybenzamine (PB) preparation for resection of a pheochromocytoma. Intraoperative magnesium sulfate infusion (+MgSO(4)) is often used for its cardiovascular stabilizing properties. We hypothesized that preparation with NC would be similar clinically to PB for resection of a pheochromocytoma, and MgSO(4) infusion would not affect intraoperative stability.This retrospective review included 64 patients undergoing resections of a pheochromocytoma from 2003 to 2011. PB or NC was used preoperatively, with MgSO(4) use distributed equally in the population. Pre-, intra-, and postoperative hemodynamics and outcomes were compared.There was no difference in NC (n = 7) versus PB (n = 57) or +MgSO(4) (n = 33) versus -MgSO(4) (n = 31) groups for demographics with the exception of age. The NC group had smaller median tumor size and lesser plasma baseline levels of normetanephrine than the PB group, but subgroup analysis of all neoplasms3.0 cm revealed no differences. Pre-, intra- and postoperative hemodynamic stability and outcomes were similar for NC versus PB analyses as well as the +MgSO(4) versus -MgSO(4) groups.NC use may be an equivalent alternative to PB in preoperative preparation, especially for smaller pheochromocytomas. Intraoperative MgSO(4) use does not seem to have a substantive effect on hemodynamic stability. more...
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- 2012
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31. Synthesis and Characterizations of Lithium Vanadium Oxide Nanotubes
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Juan-Ping Sun, Ming-Xia Zhang, Guang-Ming Wu, Hui-Yu Yang, Jun Shen, and Chaojun Cui
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Inorganic Chemistry ,Materials science ,chemistry ,Inorganic chemistry ,chemistry.chemical_element ,General Materials Science ,Lithium ,Vanadium oxide - Published
- 2009
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32. Conditioning Vaccination Site With Irradiated MIP-3α–transfected Tumor Cells Enhances Efficacy of Dendritic Cell-based Cancer Vaccine
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Yi-Chuan Yao, Hui-Ting Cheng, Yun-Han Zhu, Ko-Jiunn Liu, Hui-Yu Yang, Neng-Yao Shih, Yu-Chen Wu, and Yi-Mei Hung
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Cancer Research ,Chemokine ,Lung Neoplasms ,medicine.medical_treatment ,Immunology ,Melanoma, Experimental ,Inflammation ,Transfection ,Cancer Vaccines ,Mice ,Immune system ,Cell Movement ,Cell Line, Tumor ,medicine ,Animals ,Immunology and Allergy ,Antigen-presenting cell ,Pharmacology ,Chemokine CCL20 ,biology ,Chemistry ,Dendritic Cells ,Immunotherapy ,Dendritic cell ,Mice, Inbred C57BL ,biology.protein ,Cancer vaccine ,medicine.symptom - Abstract
Macrophage inflammation protein-3alpha (MIP-3alpha) is a chemokine expressed in inflamed tissue and capable of inducing migration of immature dendritic cells (DCs) or Langerhans cells. We postulated that conditioning vaccination sites with MIP-3alpha might enhance the efficacy of subsequently administered DC-based cancer vaccines. Our results demonstrate that subcutaneously injection of irradiated tumor cells expressing MIP-3alpha induces substantial cell infiltration to the injection site. Vaccination of irradiated tumor cells expressing MIP-3alpha followed by DCs pulsed with irradiated tumor cells can effectively suppress tumor growth in animals, which is significantly better than vaccination with irradiated MIP-3alpha-producing tumor cells or DCs pulsed with tumor cells alone. The protective effect was most evident when the MIP-3alpha-producing tumor cells and DC-based vaccines were injected at the same site. These results support the notion that this combination vaccination strategy might generate a more effective immune response to suppress the growth of tumor cells in animals. more...
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- 2009
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33. PD34-03 TIMING OF THE CONFIRMATORY BIOPSY IN PROSTATE CANCER ACTIVE SURVEILLANCE: ANALYSIS OF THE CANARY PROSTATE CANCER ACTIVE SURVEILLANCE STUDY (PASS)
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Peter R. Carroll, Raymond S. Lance, Hui-Yu Yang, Andrew A. Wagner, Lisa F. Newcomb, James D. Brooks, Ian M. Thompson, John T. Wei, Yingye Zheng, William J. Ellis, Peter S. Nelson, Martin E. Gleave, Liam C. Macleod, and Daniel W. Lin more...
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Oncology ,medicine.medical_specialty ,Surveillance study ,medicine.diagnostic_test ,business.industry ,Urology ,Logistic regression ,medicine.disease ,Prostate cancer ,medicine.anatomical_structure ,Prostate ,Internal medicine ,Cohort ,Biopsy ,medicine ,In patient ,Analysis factors ,business - Abstract
INTRODUCTION AND OBJECTIVES: Active surveillance (AS) is gaining acceptance for men with low risk prostate cancer (PCa) to prevent over-treatment related morbidity. Variability exists regarding the timing of the confirmatory biopsy (CBx) after diagnostic biopsy (DBx) in AS protocols. We analyze the Prostate Cancer Active Surveillance (PASS) cohort, determining timing and rates of PCa adverse reclassification (henceforth called reclassification) on CBx. METHODS: Men (N1⁄4422) with low risk PCA on AS were selected from the multicenter PASS cohort (1,067 screened). Criteria included enrollment within 1 year of DBx, receipt of CBx after enrollment, DBx with 1⁄47 or to >1⁄434% cores involved. The PASS protocol requires a CBx within the first 6-12 months of DBx. The time interval from DBx to CBx in tertiles was 0-8.8, 8.9-12.4 and > 12.5 months. For simplicity, rates of reclassification on CBx were calculated at 13 months post-DBx. Multivariable logistic regression determined association between reclassification, CBx timing, and other clinical and pathologic parameters. RESULTS: 90 men (21.3%) experienced reclassification on CBx. CBx was performed 11 months (median) after DBx (IQR 7.8-13.8). Rates of reclassification at 13 months were 23.5% (28/ 199 CBx), 19.5% (34/174 CBx), and 21.7% (28/129 CBx), respectively (p1⁄40.71). On multivariable analysis factors associated with reclassification were BMI and prostate volume. BMI (categorized as 35 kg/m (OR 6.1 95% CI 2.0, 18.7) compared to those with BMI < 30 kg/m. Moderate (30-60ml; OR 0.28 95% CI 0.14, 0.57) and large (60mlþ; OR 0.14 95% CI 0.05, 0.40) prostate volume were associated with decreased odds of reclassification compared to volume more...
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- 2015
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34. A pilot clinical trial of vaccination with dendritic cells pulsed with autologous tumor cells derived from malignant pleural effusion in patients with late-stage lung carcinoma
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Jacqueline Whang-Peng, Shin-Hun Juang, Gee-Chen Chang, Haw-Chang Lan, Hui-Yu Yang, R N Hui-Chen Lee, Yu-Chen Wu, Liu Ko-Jiunn, and Yi-Mei Hung
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Adult ,Male ,Cancer Research ,Pathology ,medicine.medical_specialty ,Lung Neoplasms ,Pleural effusion ,T-Lymphocytes ,medicine.medical_treatment ,Pilot Projects ,Cancer Vaccines ,Pleural disease ,Antigens, Neoplasm ,Carcinoma, Non-Small-Cell Lung ,medicine ,Carcinoma ,Humans ,Malignant pleural effusion ,Lung cancer ,Aged ,Tumor Necrosis Factor-alpha ,business.industry ,Respiratory disease ,Granulocyte-Macrophage Colony-Stimulating Factor ,Dendritic Cells ,Immunotherapy ,Middle Aged ,Flow Cytometry ,medicine.disease ,Coculture Techniques ,Pleural Effusion, Malignant ,Oncology ,Pleurisy ,Female ,Interleukin-4 ,Lymphocyte Culture Test, Mixed ,business - Abstract
BACKGROUND The authors conducted a pilot clinical trial to explore the vaccination of patients with late-stage lung carcinoma with dendritic cells (DCs) pulsed with necrotic tumor cells derived from malignant pleural effusion specimens, and to evaluate the antitumor immune response induced by this therapy. METHODS Autologous DCs were generated by culturing adherent mononuclear cells with interleukin-4 and granulocyte-macrophage–colony-stimulating factor for 7 days. Day-7 DCs were cocultured overnight with autologous necrotic tumor cells derived from pleural effusion specimens to allow internalization of tumor antigens. DCs were then treated with tumor necrosis factor-alpha for 16 hours. The antigen-loaded DCs were injected into each patient's inguinal lymph nodes under sonographic guidance. Eight patients with late-stage nonsmall cell lung carcinoma were treated in this manner. Patients were vaccinated once weekly for 4 weeks and then boosted twice biweekly. RESULTS The authors found that there was no Grade II/III toxicity and autoimmune response in all patients after intranodal injection of the DC vaccine. Minor to moderate increases in T-cell responses against tumor antigens were observed after DC vaccination in six of eight patients. Five patients had progressive disease. One patient had minor tumor response and two patients had stable disease. The two patients who had longer disease control also had better T-cell responses. CONCLUSIONS The results indicated that it was feasible to immunize patients with lung carcinoma intranodally with DCs pulsed with necrotic tumor cells enriched from pleural effusion specimens, and this approach may generate T-cell responses and provide clinical benefit in some patients. Cancer 2005. © 2005 American Cancer Society. more...
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- 2005
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35. Generation of Carcinoembryonic Antigen (CEA)-Specific T-Cell Responses in HLA-A*0201 and HLA-A*2402 Late-Stage Colorectal Cancer Patients after Vaccination with Dendritic Cells Loaded with CEA Peptides
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Li-Tzong Chen, Wei Lan Yu, Ann-Lii Cheng, Hui Yu Yang, Chuan Cheng Wang, Ko Jiunn Liu, Dong-Tsamn Lin, Yi Mei Hung, Yu Chen Wu, Jacqueline Whang-Peng, and Shin-Hun Juang
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Adult ,Male ,Cancer Research ,Time Factors ,Genotype ,T-Lymphocytes ,T cell ,medicine.medical_treatment ,HLA-A24 Antigen ,Enzyme-Linked Immunosorbent Assay ,Cancer Vaccines ,Interferon-gamma ,Carcinoembryonic antigen ,Immune system ,HLA-A2 Antigen ,medicine ,Humans ,Cells, Cultured ,Aged ,HLA-A Antigens ,biology ,business.industry ,HLA-A24 ,Granulocyte-Macrophage Colony-Stimulating Factor ,Cancer ,Dendritic Cells ,Immunotherapy ,Middle Aged ,Flow Cytometry ,medicine.disease ,Carcinoembryonic Antigen ,Granulocyte macrophage colony-stimulating factor ,medicine.anatomical_structure ,Oncology ,Immunology ,Leukocytes, Mononuclear ,biology.protein ,Female ,Colorectal Neoplasms ,Peptides ,Oncofetal antigen ,business ,medicine.drug - Abstract
Purpose: We intranodally immunized metastatic colorectal carcinoma patients, who had failed standard chemotherapy, with dendritic cells (DCs) pulsed with HLA-A*0201- or HLA-A*2402-restricted carcinoembryonic antigen (CEA) peptides to evaluate the safety of this treatment and the immune response against CEA peptides before and after the treatment. Experimental Design: Six patients with the HLA-A*2402 genotype and 4 patients with the HLA-A*0201 genotype were enrolled. A single CEA peptide (YLSGANLNL) or two CEA peptides (QYSWFVNGTF and TYACFVSNL) were used for patients with the HLA-A*0201 or HLA-A*2402 genotype, respectively. Autologous DCs were generated by culturing adherent mononuclear cells with interleukin 4 and granulocyte macrophage colony-stimulating factor for 6 days. Maturation of DCs was then induced with tumor necrosis factor α for 40 h. Mature DCs were pulsed with appropriate CEA peptides for 2 h. After washing, 1 million peptide-pulsed DCs were injected into one inguinal lymph node under sonographic guidance. Each patient received four injections. Results: No grade II/III toxicity or autoimmunity was observed. An increase in the number of CEA-specific T cells after DC vaccination could be detected in 7 of 10 (70%) patients. Two (20%) patients had stable disease for at least 12 weeks. One of these 2 patients experienced a transient decrease in CEA levels during the treatment period and also had the most significant T-cell response against the immunizing CEA peptides. Conclusions: These results suggest that our vaccination procedure can generate or boost specific T-cell responses and may provide clinical benefit in certain cancer patients. more...
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- 2004
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36. Does multimedia support individual differences? – EFL learners’ listening comprehension and cognitive load
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Hui-Yu Yang
- Subjects
Teaching method ,media_common.quotation_subject ,Cognition ,Linguistics ,Education ,Language proficiency ,Aptitude ,Psychology ,Visual learning ,Cognitive load ,Cognitive psychology ,Cognitive style ,Coding (social sciences) ,media_common - Abstract
The present study examines how display model, English proficiency and cognitive preference affect English as a Foreign Language (EFL) learners’ listening comprehension of authentic videos and cognitive load degree. EFL learners were randomly assigned to one of two groups. The control group received single coding and the experimental group received dual coding. The results show that the display model affects EFL learners’ cognitive load and listening comprehension of CNN news. There is no interaction among English proficiency, cognitive preference and experimental conditions on the listening comprehension scores and cognitive load scale. Learners with a low aptitude for visual learning had poorer performance and a higher cognitive load than those with a high aptitude for visual learning. All learners, regardless of cognitive styles and level of English, benefited from dual coding. The dual-code display model thus seems to be effective in reducing EFL learners’ cognitive load. more...
- Published
- 2014
- Full Text
- View/download PDF
37. Yeast osmosensors Hkr1 and Msb2 activate the Hog1 MAPK cascade by different mechanisms
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Haruo Saito, Kazuo Tatebayashi, Akiko Nishimura, Keiichiro Tanaka, Hui-Yu Yang, and Katsuyoshi Yamamoto
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Scaffold protein ,Saccharomyces cerevisiae Proteins ,MAP Kinase Signaling System ,Saccharomyces cerevisiae ,MAPK cascade ,Biology ,Protein Serine-Threonine Kinases ,Biochemistry ,Osmotic Pressure ,Cytoskeleton ,Protein kinase A ,Molecular Biology ,Adaptor Proteins, Signal Transducing ,Intracellular Signaling Peptides and Proteins ,Membrane Proteins ,Cell Biology ,Actin cytoskeleton ,MAP Kinase Kinase Kinases ,Transmembrane protein ,Cell biology ,Protein Structure, Tertiary ,PXXP Motif ,Mitogen-Activated Protein Kinases ,Proto-oncogene tyrosine-protein kinase Src ,Protein Binding - Abstract
To cope with environmental high osmolarity, the budding yeast Saccharomyces cerevisiae activates the mitogen-activated protein kinase (MAPK) Hog1, which controls an array of osmoadaptive responses. Two independent, but functionally redundant, osmosensing systems involving the transmembrane sensor histidine kinase Sln1 or the tetraspanning membrane protein Sho1 stimulate the Hog1 MAPK cascade. Furthermore, the Sho1 signaling branch itself also involves the two functionally redundant osmosensors Hkr1 and Msb2. However, any single osmosensor (Sln1, Hkr1, or Msb2) is sufficient for osmoadaptation. We found that the signaling mechanism by which Hkr1 or Msb2 stimulated the Hog1 cascade was specific to each osmosensor. Specifically, activation of Hog1 by Msb2 required the scaffold protein Bem1 and the actin cytoskeleton. Bem1 bound to the cytoplasmic domain of Msb2 and thus recruited the kinases Ste20 and Cla4 to the membrane, where either of them can activate the kinase Ste11. The cytoplasmic domain of Hkr1 also contributed to the activation of Ste11 by Ste20, but through a mechanism that involved neither Bem1 nor the actin cytoskeleton. Furthermore, we found a PXXP motif in Ste20 that specifically bound to the Sho1 SH3 (Src homology 3) domain. This interaction between Ste20 and Sho1 contributed to the activation of Hog1 by Hkr1, but not by Msb2. These differences between Hkr1 and Msb2 may enable differential regulation of these two proteins and provide a mechanism through Msb2 to connect regulation of the cytoskeleton with the response to osmotic stress. more...
- Published
- 2014
38. The effect of acute exposure to coarse particulate matter air pollution in a rural location on circulating endothelial progenitor cells: results from a randomized controlled study
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Lu Wang, Robert D. Brook, Elif A. Oral, Bhramar Mukherjee, Hui Yu Yang, J. Timothy Dvonch, Sanjay Rajagopalan, Srilakshmi Yalavarthi, Mariana J. Kaplan, Qinghua Sun, Jack R. Harkema, Jeffrey R. Brook, Catherine Spino, Masako Morishita, and Robert L. Bard more...
- Subjects
Adult ,Male ,Rural Population ,Health, Toxicology and Mutagenesis ,Inflammation ,Biology ,Toxicology ,Endothelial progenitor cell ,Article ,Andrology ,chemistry.chemical_compound ,Young Adult ,White blood cell ,medicine ,Humans ,Progenitor cell ,Particle Size ,Cross-Over Studies ,Stem Cells ,Endothelial Cells ,Venous blood ,Crossover study ,Vascular endothelial growth factor ,medicine.anatomical_structure ,chemistry ,Immunology ,Female ,Particulate Matter ,medicine.symptom ,Stem cell - Abstract
Fine particulate matter (PM) air pollution has been associated with alterations in circulating endothelial progenitor cell (EPC) levels, which may be one mechanism whereby exposures promote cardiovascular diseases. However, the impact of coarse PM on EPCs is unknown.We aimed to determine the effect of acute exposure to coarse concentrated ambient particles (CAP) on circulating EPC levels.Thirty-two adults (25.9 ± 6.6 years) were exposed to coarse CAP (76.2 ± 51.5 μg m(-3)) in a rural location and filtered air (FA) for 2 h in a randomized double-blind crossover study. Peripheral venous blood was collected 2 and 20 h post-exposures for circulating EPC (n = 21), white blood cell (n = 24) and vascular endothelial growth factor (VEGF) (n = 16-19) levels. The changes between exposures were compared by matched Wilcoxon signed-rank tests.Circulating EPC levels were elevated 2 [108.29 (6.24-249.71) EPC mL(-1); median (25th-75th percentiles), p = 0.052] and 20 h [106.86 (52.91-278.35) EPC mL(-1), p = 0.008] post-CAP exposure compared to the same time points following FA [38.47 (0.00-84.83) and 50.16 (0.00-104.79) EPC mL(-1)]. VEGF and white blood cell (WBC) levels did not differ between exposures.Brief inhalation of coarse PM from a rural location elicited an increase in EPCs that persisted for at least 20 h. The underlying mechanism responsible may reflect a systemic reaction to an acute "endothelial injury" and/or a circulating EPC response to sympathetic nervous system activation. more...
- Published
- 2013
39. [Glucagon like peptide-1 inhibits high glucose-induced injury of oxidative stress in cardiomyocytes of neonatal rats]
- Author
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Yun-Fei, Bian, Dong-Xue, Wang, Hui-Yu, Yang, and Chuan-Shi, Xiao
- Subjects
Male ,Apoptosis ,Protective Agents ,Rats ,Rats, Sprague-Dawley ,Oxidative Stress ,Phosphatidylinositol 3-Kinases ,Glucose ,Animals, Newborn ,Glucagon-Like Peptide 1 ,Animals ,Female ,Myocytes, Cardiac ,Proto-Oncogene Proteins c-akt ,Cells, Cultured ,Signal Transduction - Abstract
The present study was to investigate the effect of glucagon like peptide-1 (GLP-1) on high glucose-induced oxidative stress of cardiomyocytes and the possible role of the PI3K-Akt signal path in this process in the neonatal SD rats. With enzymatic digestion and immunofluorescence identification, cardiomyocytes after 72-96 h of primary culture were used in experiment. The cells were divided into 5 groups: normal control group, high glucose group, high glucose + GLP-1 group, high glucose + GLP-1 + LY294002 group and high osmolarity control group. The content of MDA was detected by TBA colouration method. The content of SOD was detected by xanthine oxidase method. The change of NADPH P47phox subunit mRNA quantity was detected by PCR gel electrophoresis. The level of ROS was detected by flow cytometry, and was also observed by fluorescence microscope. The DNA ladder was examined by agarose gel electrophoresis, and the cell apoptosis was determined by Annexin-V-FITC/PI flow cytometry, and the phosphorylation of Akt was determined by Western blotting. Compared with those in the normal control group, in the high glucose group, the cells grew poorly, and the beating rate was significantly lower (P0.05); The apoptotic rate was significantly increased (P0.05); The MDA content was increased (P0.05); It showed the typical DNA ladder, which is the characteristic of apoptosis; The SOD activity was decreased (P0.05); The level of intracellular ROS increased (P0.05); And the expression of NADPH P47phox subunit mRNA was increased; However the phosphorylation level of Akt was decreased. Pretreatment with GLP-1 improved the above-mentioned parameters and decreased the expression of NADPH P47phox subunit mRNA (P0.05). However, compared with the high glucose + GLP-1 group, LY294002, an inhibitor of PI3K-Akt signal path, attenuated the protective effect of GLP-1 in the high glucose + GLP-1 + LY294002 group. It is suggested that GLP-1 plays a protective role in the high glucose-induced injury and apoptosis of cardiomyocytes, and the PI3K-Akt signal path is involved in this process. more...
- Published
- 2011
40. Application of Fuzzy Genetic Algorithm in Control for Heating Furnace
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Hui-yu Yang, Yan-jun Ren, and Xiao-hui Li
- Subjects
Temperature control ,Control theory ,Computer science ,Control system ,Genetic algorithm ,Fuzzy control system ,Defuzzification ,Fuzzy logic ,Membership function - Abstract
The temperature control system of heating furnace is a complex system. As in various practical complex control systems of heating furnace thermal process parameters, the traditional fuzzy controller can hardly ensure that the system controlling effect kept in ideal state all the time. Aimed at existent problem, the paper proposed a parameter optimization method based on fuzzy control adjustment genetic algorithm. With the genetic algorithm, the self-correction and self-adjustment of the fuzzy membership function curve, the parameters p, q of the fuzzy control analytic expression U = f(E,C,p,q) and the output club-shaped membership function position can be carried out, thereby the fuzzy control which coincides with experience is realized. The controlling precision and stability are greatly improved. Not only the system reaches controlling quality but also has better controlling quality. more...
- Published
- 2009
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41. Application of Tread Patterns Noise-Reduction Based on Fuzzy Genetic Algorithm
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Hui-yu Yang, Xiao-hui Li, and Bing Guo
- Subjects
Noise ,Fitness function ,Computer science ,Noise reduction ,Genetic algorithm ,Environmental pollution ,Tread ,Algorithm ,Fuzzy logic ,Block (data storage) - Abstract
With the growing number of cars, how to effectively reduce tire noise on the environmental pollution has become an urgent problem. The Fuzzy Genetic Noise-Reduction Algorithm, FGNRA, was proposed based on the analytic result of pattern parameters, which include the width and length of pattern block, the width length and direction of pattern slot, the number of pattern strip the basic number of pattern pitch, the pitch array and the pattern misplacement. The method of fuzzy noise tread patterns and the use of genetic algorithm to optimize the structural parameters to achieve the purpose of reducing tire noise, and a low-noise tread patterns structure were proposed at the same time. The research results have been successfully applied to the design of way for the low- noise tread patterns. more...
- Published
- 2009
- Full Text
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42. Glycosylation defects activate filamentous growth Kss1 MAPK and inhibit osmoregulatory Hog1 MAPK
- Author
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Hui-Yu Yang, Haruo Saito, Katsuyoshi Yamamoto, and Kazuo Tatebayashi
- Subjects
MAPK/ERK pathway ,Glycosylation ,Saccharomyces cerevisiae Proteins ,Mutant ,Protein tyrosine phosphatase ,Biology ,Mannosyltransferases ,Models, Biological ,General Biochemistry, Genetics and Molecular Biology ,Article ,chemistry.chemical_compound ,Saccharomyces ,Gene Expression Regulation, Fungal ,Enzyme Inhibitors ,Molecular Biology ,General Immunology and Microbiology ,General Neuroscience ,Tunicamycin ,Cell biology ,carbohydrates (lipids) ,chemistry ,Membrane protein ,Biochemistry ,Mitogen-activated protein kinase ,biology.protein ,Signal transduction ,Mitogen-Activated Protein Kinases ,Protein Tyrosine Phosphatases - Abstract
The yeast filamentous growth (FG) MAP kinase (MAPK) pathway is activated under poor nutritional conditions. We found that the FG-specific Kss1 MAPK is activated by a combination of an O-glycosylation defect caused by disruption of the gene encoding the protein O-mannosyltransferase Pmt4, and an N-glycosylation defect induced by tunicamycin. The O-glycosylated membrane proteins Msb2 and Opy2 are both essential for activating the FG MAPK pathway, but only defective glycosylation of Msb2 activates the FG MAPK pathway. Although the osmoregulatory HOG (high osmolarity glycerol) MAPK pathway and the FG MAPK pathway share almost the entire upstream signalling machinery, osmostress activates only the HOG-specific Hog1 MAPK. Conversely, we now show that glycosylation defects activate only Kss1, while activated Kss1 and the Ptp2 tyrosine phosphatase inhibit Hog1. In the absence of Kss1 or Ptp2, however, glycosylation defects activate Hog1. When Hog1 is activated by glycosylation defects in ptp2 mutant, Kss1 activation is suppressed by Hog1. Thus, the reciprocal inhibitory loop between Kss1 and Hog1 allows only one or the other of these MAPKs to be stably activated under various stress conditions. more...
- Published
- 2008
43. Transmembrane mucins Hkr1 and Msb2 are putative osmosensors in the SHO1 branch of yeast HOG pathway
- Author
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Haruo Saito, Kazuo Tatebayashi, Katsuyoshi Yamamoto, Keiichiro Tanaka, Hui-Yu Yang, Yusaku Matsushita, Midori Imai, and Taichiro Tomida
- Subjects
Saccharomyces cerevisiae Proteins ,General Immunology and Microbiology ,Osmotic concentration ,General Neuroscience ,Mutant ,GTPase-Activating Proteins ,Intracellular Signaling Peptides and Proteins ,Membrane Proteins ,Saccharomyces cerevisiae ,Cell cycle ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Transmembrane protein ,Yeast ,Article ,Cell biology ,Microscopy, Fluorescence ,Osmotic Pressure ,Gene expression ,Mutation ,Signal transduction ,Molecular Biology ,Intracellular - Abstract
To cope with life-threatening high osmolarity, yeast activates the high-osmolarity glycerol (HOG) signaling pathway, whose core element is the Hog1 MAP kinase cascade. Activated Hog1 regulates the cell cycle, protein translation, and gene expression. Upstream of the HOG pathway are functionally redundant SLN1 and SHO1 signaling branches. However, neither the osmosensor nor the signal generator of the SHO1 branch has been clearly defined. Here, we show that the mucin-like transmembrane proteins Hkr1 and Msb2 are the potential osmosensors for the SHO1 branch. Hyperactive forms of Hkr1 and Msb2 can activate the HOG pathway only in the presence of Sho1, whereas a hyperactive Sho1 mutant activates the HOG pathway in the absence of both Hkr1 and Msb2, indicating that Hkr1 and Msb2 are the most upstream elements known so far in the SHO1 branch. Hkr1 and Msb2 individually form a complex with Sho1, and, upon high external osmolarity stress, appear to induce Sho1 to generate an intracellular signal. Furthermore, Msb2, but not Hkr1, can also generate an intracellular signal in a Sho1-independent manner. more...
- Published
- 2007
44. RETRACTED ARTICLE: The Effect of Attention Cueing on Science Text Learning
- Author
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Che-Chang Chang and Hui-Yu Yang
- Subjects
05 social sciences ,Educational technology ,050301 education ,Science education ,050105 experimental psychology ,Computer Science Applications ,Education ,Human-Computer Interaction ,Mathematics (miscellaneous) ,Text learning ,Mathematics education ,0501 psychology and cognitive sciences ,Psychology ,0503 education ,Cognitive psychology - Published
- 2015
- Full Text
- View/download PDF
45. The Application of KINECT Motion Sensing Technology in Game-Oriented Study
- Author
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Liang Ming Xu, Hao Zhang, Hui Yu Yang, Ping Jian Zhang, and Wei Xu
- Subjects
lcsh:T58.5-58.64 ,Multimedia ,lcsh:Information technology ,Computer science ,Learning environment ,General Engineering ,Motion sensing ,Economic shortage ,Roll play ,computer.software_genre ,Education ,Motion sensing interaction ,Mode (computer interface) ,Game-oriented learning ,KINECT ,lcsh:L ,computer ,lcsh:Education - Abstract
The learning environment based on the KINECT Motion Sensing technology is able to fully mobilize the learners' multi-sensory organs, closely combine study with sports and enhance human-computer interactions, which can be conducive to the learners' health, greatly increase the relishes of learning and promote effective learning in the game, and finally compensate for the shortage of human-computer interactions in the traditional mouse and keyboard mode. The article elaborates on the KINECT Motion Sensing Technology and its educational applications status by analyzing its effective supports for game-oriented studying environment, based on which the article establishes a game-oriented learning environment. Eventually the article reveals an applicable case of game-oriented teaching and learning as a reference for related researches. more...
- Published
- 2014
- Full Text
- View/download PDF
46. Abrin triggers cell death by inactivating a thiol-specific antioxidant protein
- Author
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Hui-Yu Yang, Yu-Hauh Wu, Jung-Yaw Lin, Sheue-Fang Shih, and Chih-Hung Hung
- Subjects
Programmed cell death ,Peroxiredoxin III ,Molecular Sequence Data ,Apoptosis ,Cytochrome c Group ,Saccharomyces cerevisiae ,Mitochondrion ,Biochemistry ,Antioxidants ,Cell Line ,chemistry.chemical_compound ,Jurkat Cells ,Humans ,Amino Acid Sequence ,Cloning, Molecular ,Molecular Biology ,chemistry.chemical_classification ,Reactive oxygen species ,biology ,Cell Death ,Sequence Homology, Amino Acid ,Cytochrome c ,Membrane Proteins ,Biological Transport ,Cell Biology ,Glutathione ,Peroxiredoxins ,Molecular biology ,Recombinant Proteins ,Cell biology ,Mitochondria ,Neoplasm Proteins ,Cytosol ,Kinetics ,chemistry ,Amino Acid Substitution ,Peroxidases ,biology.protein ,Abrin ,Sequence Alignment ,Intracellular ,HeLa Cells - Abstract
Abrin A-chain (ABRA) inhibits protein synthesis by its N-glycosidase activity as well as induces apoptosis, but the molecular mechanism of ABRA-induced cell death has been obscure. Using an ABRA mutant that lacks N-glycosidase activity as bait in a yeast two-hybrid system, a 30-kDa antioxidant protein-1 (AOP-1) was found to be an ABRA(E164Q)-interacting protein. The interaction was further confirmed in vitro by a glutathione S-transferase pull-down assay. The colocalization of endogenous AOP-1 and exogenous ABR proteins in the cell was demonstrated by confocal immunofluorescence. We also demonstrated that ABRA attenuates AOP-1 antioxidant activity in a dose-dependent manner and the intracellular level of reactive oxygen species (ROS) increases in ABR-treated cells. Moreover, ROS scavengers N-acetylcysteine and 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl delayed programmed cell death. This indicates that ROS are important mediators of ABR-induced apoptosis. When ectopically expressed, AOP-1 blocked the release of cytochrome c and prevented apoptosis in ABR-treated cells. These findings suggest that the binding of ABRA to AOP-1 promotes apoptosis by inhibiting the mitochondrial antioxidant protein AOP-1, resulting in the increase of intracellular ROS and the release of cytochrome c from the mitochondria to the cytosol, which activates caspase-9 and caspase-3. more...
- Published
- 2001
47. An exceptionally stable helix from the ribosomal protein L9: implications for protein folding and stability
- Author
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Brian Kuhlman, Hui Yu Yang, Daniel P. Raleigh, Robert Fairman, and Judith A. Boice
- Subjects
Ribosomal Proteins ,Protein Folding ,Magnetic Resonance Spectroscopy ,Collagen helix ,Morpholines ,Molecular Sequence Data ,Static Electricity ,Beta helix ,Buffers ,Protein Structure, Secondary ,Pi helix ,Geobacillus stearothermophilus ,Bacterial Proteins ,Structural Biology ,310 helix ,Amino Acid Sequence ,Molecular Biology ,Protein secondary structure ,Polyproline helix ,Chemistry ,Circular Dichroism ,Hydrogen-Ion Concentration ,Solutions ,Crystallography ,Helix ,Protein folding - Abstract
The ribosomal protein L9 has an unusual structure comprising two compact globular domains connected by a 34 residue alpha-helix. The middle 17 residues of the helix are exposed to solvent while the first seven pack against and form part of the N-terminal domain, and the last ten form part of the C-terminal domain. Here we report results which show that a peptide corresponding to the central helix of L9 is monomeric in aqueous solution and85% helical at 1 degrees C and 68(+/-7)% helical at 25 degrees C. This is considerably more helical than any other protein fragment studied to date. Another peptide corresponding to the middle 17 residues of the helix is monomeric and is 41(+/-4)% helical at 1 degrees C. Because the central helix has high intrinsic stability the globular N and C-terminal domains will likely be stabilized by their interactions with the helix. Therefore, the stability of the two terminal domains should not be completely independent because both domains gain stability from a shared structural element, the central helix. Also, the ability of the central helix to form native-like structure in isolation highlights a potential role for the helix in the early stages of the folding process. more...
- Published
- 1997
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