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Background and AimNon-alcoholic fatty liver disease (NAFLD) has become the most common chronic liver disease in children. With the continuous emergence of various non-invasive diagnostic methods, imaging techniques have always been considered as potential alternative methods to liver biopsy. This study aimed to evaluate the diagnostic performance of imaging techniques so as to search for the most promising technology.MethodsWe searched English and Chinese databases. English databases included Cochran library, Embase, PubMed, and Web of Science, while Chinese databases included the Wanfang database and China National Knowledge Internet.ResultsFinally, 11 articles were included (12 studies, one of which included studies on both fibrosis and steatosis). Further, 26.2% of the participants had mild steatosis, 34.1% had moderate steatosis, and 34.9% had severe steatosis. Also, 64.0% had any fibrosis, 29.1% had significant fibrosis, 13.8% had advanced fibrosis, and 2.8% had cirrhosis. Irrespective of the grade of fibrosis, transient elastography (TE) had higher sensitivity (97–100%), whereas magnetic resonance elastography (MRE) had the lowest sensitivity (58–63%). The pooled sensitivity and specificity of imaging techniques in diagnosing steatosis were 89% (95% CI, 71–96) and 89% (95% CI, 72–96), and AUROC 0.95 (95% CI, 93–97), multifrequency magnetic resonance elastography-hepatic fat fraction (mMRE-HFF) had the highest sensitivity (87%, 95% CI 77–97), ultrasonography (US) had the lowest specificity (96%, 95% CI 92–98%).ConclusionImaging techniques have a good diagnostic performance for children with NAFLD, especially the diagnosis of liver fibrosis based on ultrasound or magnetic resonance elastography. Compared with different imaging techniques, TE has the best performance in diagnosing significant fibrosis. Liver stiffness measurement (LSM) is expected to become a biological indicator for routine screening, dynamic monitoring of disease changes, and prognostic evaluation.

BackgroundMost adrenal disorders have an insidious onset and are not severe. Only a minority of adrenal lesions can lead to life-threatening cardiovascular crisis. Venoarterial extracorporeal membrane oxygenation (VA-ECMO) is an extracorporeal life support technology that can partially replace human cardiopulmonary function. To date, many case reports have indicated that this technology can temporarily help patients survive critical condition and to achieve a good prognosis. Case presentation: A 27-year-old male patient presented to the emergency department with sudden dyspnoea and haemoptysis. Pulmonary oedema, cardiogenic shock and cardiac arrest occurred immediately after admission, prompting VA-ECMO treatment. After the patient’s haemodynamics were stabilized, multiple endocrine neoplasia type 2 (MEN2) was detected via laboratory tests and imaging examination. After the withdrawal of VA-ECMO support, surgical resection of the lesions was performed, and a good prognosis was achieved.ConclusionVA-ECMO can be used as an emergency treatment for refractory cardiogenic shock caused by adrenal lesions. However, a large number of prospective studies are still needed to confirm its safety and efficacy and to clarify the timing of initiation and assessment of patient prognosis.

To understand the effects of the eIF-5A gene in response to abiotic stress, the 909 bp full-length cDNA of eIF-5A, including a 480 bp open reading frame encoding 159 amino-acid (aa) residues, was isolated from the leaf of herbal plant (Apocynum venetum) by rapid-amplification of cDNA ends. The deduced molecular weight of the encoding protein was 17.48 kDa with a theoretical pI of 5.61 and predicted no signal peptide. Real-time polymerase chain reaction analysis revealed that AveIF-5A gene expression was induced by cold, salt and drought stress. To determine the biological function of this gene, recombinant plasmids expressing AveIF-5A and AveIF-5A1 (86–156 aa truncated polypeptide) were used to transform Escherichia coli. The analysis of the growth curves of recombinant E. coli revealed that AveIF-5A and AveIF-5A1 improved the resistance of transformed E. coli to low-temperature, drought and salt stress. These results could provide experimental evidence of the function of the AveIF-5A gene as a valuable gene resource in plant resistance-breeding.

A pinhole camera for imaging X-ray synchrotron radiation from a dipole magnet is now in operation at the Shanghai Synchrotron Radiation Facility (SSRF) storage ring. The electron beam size is derived by unfolding the radiation image and the point spread function (PSF) with deconvolution techniques. The performance of the pinhole is determined by the accuracy of the PSF measurement. This article will focus on a beam-based calibration scheme to measure the PSF system by varying the beam images with different quadrupole settings and fitting them with the corresponding theoretical beam sizes. Applying this method at SSRF, the PSF value of the pinhole is revised from 37 to 44 μm. The deviation in beam size between the theoretical value and the measured value is minimized to 4% after calibration. This optimization allows us to observe the horizontal disturbance due to injection down to as small as 0.5 μm.

Rapid growth of global population should be demanded more high-quality food and this might be alleviated by improving protein nutrition levels in usual foodstuff. The expense associated with food additives used for animal production have led to the study of intracellular production of high-quality protein (HQP) by transgenic crops and other organisms, as a means of obtaining efficient and less costly sources of essential amino acids. One important approach is to extract high essential amino acids from bacteria or others. We designed a HQP gene encoding about 100 amino acids which had more than 80 % essential amino acids. GOR/Gibrat and Swiss-Pdb Viewer analysis were used to predict the secondary structure of HQP-1 in pI value, hydrophilic and hydrophobic properties. Then the HQP gene was transferred into E. coli BL21 (DE3), and the protein was extracted and identified by SDS-PAGE.

In this article, comparative research on residents’ effect perception, participation capacity and willingness on Pro-poor Tourism (PPT) is given based on the questionnaire carried out in Wulong County and Fengjie County in Three Gorges Area, Chongqing, China. Some technologies, such as SPSS 13.0, ANOVA and T-test are applied to analyze the data and results show Wulong residents’ perception behavior is better than that of Fengjie residents. Moreover, the residents with different demographic characteristics have different participation behavior. Finally, multiple regression analysis is applied to identify the key factors influencing residents’ perception behavior, that is participation willingness and positive economic effect perception, positive social and cultural effect perception and participation capacity.

Through systematic summary of domestic research documents about China’s tourism-oriented poverty alleviation in recent 10 years, it is found that researches mainly focus on 5 aspects: effect of tourism-oriented poverty alleviation, model and development strategy, benefit of poverty stricken people, practice of tourism-oriented poverty alleviation in specific region, and other related problems. At present, academic circle mainly has weak points of research content, method, object and region in tourism-oriented poverty alleviation. Finally, it points out key research interests: (1) Strengthening combination of qualitative and quantitative researches; (2) Expanding research fields and scope and promoting in-depth researches; (3) Analyzing benefiting mechanism of poverty-stricken people participating in tourism development in depth with poverty-stricken people as research objects; (4) Increasing scale development of perception research on effect of tourism-oriented poverty alleviation and research on measurement testing, to make subsequent research have reliability and comparability.

Following the increase requirement of data security, distribute information after encrypting, this is an effective method to keep its security during transporting period. This article introduces an encryption and distribution system design process. This system takes a FPGA chip as a control center. Apply VHDL implements PCI protocol and DES encryption and decryption algorithm. The function of this system is to choose crude information from a computer, then, encrypt it by a PCI encryption board and store this information into a portable storage device; and the opposite process. Except that, this system can improve its program on line and exchange keys using software. In all, this system has convenience operation and flexible improvement.