11 results on '"Hofrichter, Martin"'
Search Results
2. Additional file 2: Table S1. of The genome sequence of the commercially cultivated mushroom Agrocybe aegerita reveals a conserved repertoire of fruiting-related genes and a versatile suite of biopolymer-degrading enzymes
- Author
-
Gupta, Deepak, RĂźhl, Martin, Bagdevi Mishra, Kleofas, Vanessa, Hofrichter, Martin, Herzog, Robert, Pecyna, Marek, Sharma, Rahul, Kellner, Harald, Hennicke, Florian, and Thines, Marco
- Abstract
Agrocybe aegerita AAE-3 genes in subcategories of GO term â molecular functionâ . (DOCX 31 kb)
- Published
- 2018
- Full Text
- View/download PDF
3. Additional file 3: Table S2. of The genome sequence of the commercially cultivated mushroom Agrocybe aegerita reveals a conserved repertoire of fruiting-related genes and a versatile suite of biopolymer-degrading enzymes
- Author
-
Gupta, Deepak, RĂźhl, Martin, Bagdevi Mishra, Kleofas, Vanessa, Hofrichter, Martin, Herzog, Robert, Pecyna, Marek, Sharma, Rahul, Kellner, Harald, Hennicke, Florian, and Thines, Marco
- Abstract
Agrocybe aegerita AAE-3 genes in subcategories of GO term â biological processesâ . (DOCX 31 kb)
- Published
- 2018
- Full Text
- View/download PDF
4. Bacteria inhabiting deadwood of 13 tree species are heterogeneously distributed between sapwood and heartwood
- Author
-
Moll, Julia, Kellner, Harald, Leonhardt, Sabrina, Stengel, Elisa, Dahl, Andreas, Bässler, Claus, Buscot, François, Hofrichter, Martin, and Hoppe, Björn
- Subjects
ddc - Published
- 2018
5. Additional file 6: Table S5. of The genome sequence of the commercially cultivated mushroom Agrocybe aegerita reveals a conserved repertoire of fruiting-related genes and a versatile suite of biopolymer-degrading enzymes
- Author
-
Gupta, Deepak, RĂźhl, Martin, Bagdevi Mishra, Kleofas, Vanessa, Hofrichter, Martin, Herzog, Robert, Pecyna, Marek, Sharma, Rahul, Kellner, Harald, Hennicke, Florian, and Thines, Marco
- Subjects
food and beverages - Abstract
Proteins encoded by fruiting-related genes from different agaricomycetes species. (DOCX 34 kb)
- Published
- 2018
- Full Text
- View/download PDF
6. Additional file 1: of The genome sequence of the commercially cultivated mushroom Agrocybe aegerita reveals a conserved repertoire of fruiting-related genes and a versatile suite of biopolymer-degrading enzymes
- Author
-
Gupta, Deepak, RĂźhl, Martin, Bagdevi Mishra, Kleofas, Vanessa, Hofrichter, Martin, Herzog, Robert, Pecyna, Marek, Sharma, Rahul, Kellner, Harald, Hennicke, Florian, and Thines, Marco
- Abstract
Supplementary Figures. (PDF 1068 kb)
- Published
- 2018
- Full Text
- View/download PDF
7. Additional file 5: Table S4. of The genome sequence of the commercially cultivated mushroom Agrocybe aegerita reveals a conserved repertoire of fruiting-related genes and a versatile suite of biopolymer-degrading enzymes
- Author
-
Gupta, Deepak, RĂźhl, Martin, Bagdevi Mishra, Kleofas, Vanessa, Hofrichter, Martin, Herzog, Robert, Pecyna, Marek, Sharma, Rahul, Kellner, Harald, Hennicke, Florian, and Thines, Marco
- Abstract
Annotation of Agrocybe aegerita AAE-3 genes using InterProScan. (DOCX 254 kb)
- Published
- 2018
- Full Text
- View/download PDF
8. Supplementary information from Trophic level, successional age and trait matching determine specialization of deadwood-based interaction networks of saproxylic beetles
- Author
-
Wende, Beate, Gossner, Martin M., Grass, Ingo, Arnstadt, Tobias, Hofrichter, Martin, Floren, Andreas, Linsenmair, Karl Eduard, Weisser, Wolfgang W., and Steffan-Dewenter, Ingolf
- Subjects
ComputingMethodologies_DOCUMENTANDTEXTPROCESSING - Abstract
More detailed information about experimental design and additional analyses supporting the results of the manuscript
- Published
- 2017
- Full Text
- View/download PDF
9. Regioselective and Stereoselective Epoxidation of n-3 and n-6 Fatty Acids by Fungal Peroxygenases
- Author
-
Dolores Linde, Ángel T. Martínez, Ana Gutiérrez, René Ullrich, Juan Carro, Martin Hofrichter, Alejandro González-Benjumea, European Commission, Ministerio de Ciencia e Innovación (España), Consejo Superior de Investigaciones Científicas (España), González-Benjumea, Alejandro, Linde, Dolores, Carro, Juan, Ullrich, René, Hofrichter, Martin, Martínez, Ángel T., Gutiérrez Suárez, Ana, González-Benjumea, Alejandro [0000-0003-2857-9491], Linde, Dolores [0000-0002-0359-0566], Carro, Juan [0000-0002-7556-9782], Ullrich, René [0000-0002-9165-6341], Hofrichter, Martin [0000-0001-5174-7604], Martínez, Ángel T. [0000-0002-1584-2863], and Gutiérrez Suárez, Ana [0000-0002-8823-9029]
- Subjects
Physiology ,Clinical Biochemistry ,Epoxide ,RM1-950 ,01 natural sciences ,Biochemistry ,Chemical synthesis ,Article ,Bioactive compounds ,Regioselective synthesis ,03 medical and health sciences ,chemistry.chemical_compound ,unspecific peroxygenases ,epoxylipids ,polyunsaturated fatty acids ,omega 3 (n-3) fatty acids ,omega 6 (n-6) fatty acids ,bioactive compounds ,regioselective synthesis ,stereoselective synthesis ,NMR ,chiral HPLC ,Omega 3 (n-3) fatty acids ,Chiral HPLC ,Organic chemistry ,Molecular Biology ,030304 developmental biology ,Omega 6 (n-6) fatty acids ,chemistry.chemical_classification ,Unspecific peroxygenases ,0303 health sciences ,biology ,010405 organic chemistry ,Agrocybe ,Stereoselective synthesis ,Regioselectivity ,Fatty acid ,Cell Biology ,biology.organism_classification ,3. Good health ,0104 chemical sciences ,Epoxylipids ,chemistry ,Stereoselectivity ,Polyunsaturated fatty acids ,Therapeutics. Pharmacology ,Docosanoid ,Polyunsaturated fatty acid - Abstract
17 páginas.- 7 figuras.- 2 tablas.- 53 referencias.- Supplementary Materials: The following are available online (at https://www.mdpi.com/article/10.3390/antiox10121888/s1), Epoxide metabolites from n-3 and n-6 polyunsaturated fatty acids arouse interest thanks to their physiological and pharmacological activities. Their chemical synthesis has significant drawbacks, and enzymes emerge as an alternative with potentially higher selectivity and greener nature. Conversion of eleven eicosanoid, docosanoid, and other n-3/n-6 fatty acids into mono-epoxides by fungal unspecific peroxygenases (UPOs) is investigated, with emphasis on the Agrocybe aegerita (AaeUPO) and Collariella virescens (rCviUPO) enzymes. GC-MS revealed the strict regioselectivity of the n-3 and n-6 reactions with AaeUPO and rCviUPO, respectively, yielding 91%-quantitative conversion into mono-epoxides at the last double bond. Then, six of these mono-epoxides were obtained at mg-scale, purified and further structurally characterized by 1H, 13C and HMBC NMR. Moreover, chiral HPLC showed that the n-3 epoxides were also formed (by AaeUPO) with total S/R enantioselectivity (ee > 99%) while the n-6 epoxides (from rCviUPO reactions) were formed in nearly racemic mixtures. The high regio- and enantioselectivity of several of these reactions unveils the synthetic utility of fungal peroxygenases in fatty acid epoxidation., Funding: This research was funded by BioBased Industries Joint Undertaking under the European Union’s Horizon 2020 Research and Innovation Programme, grant number 792063 (SusBind project; https://susbind.eu, accessed on 19 October 2021; to A.G., A.T.M. and M.H.), the BIO2017-86559-R and PID2020-118968RB-100 projects of the Spanish Ministry of Science & Innovation (co-financed by FEDER funds) to A.T.M. and A.G., respectively; the CSIC projects PIE-202040E185 (to A.G.) and PIE-202120E019 (to A.T.M.); and the CSIC SusPlast platform (to A.T.M.).
- Published
- 2021
10. Engineering a Highly Regioselective Fungal Peroxygenase for the Synthesis of Hydroxy Fatty Acids
- Author
-
Patricia Gomez de Santos, Alejandro González‐Benjumea, Angela Fernandez‐Garcia, Carmen Aranda, Yinqi Wu, Andrada But, Patricia Molina‐Espeja, Diana M. Maté, David Gonzalez‐Perez, Wuyuan Zhang, Jan Kiebist, Katrin Scheibner, Martin Hofrichter, Katarzyna Świderek, Vicent Moliner, Julia Sanz‐Aparicio, Frank Hollmann, Ana Gutiérrez, Miguel Alcalde, European Commission, Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), Comunidad de Madrid, Ministerio de Ciencia, Innovación y Universidades (España), Gómez de Santos, Patricia, González-Benjumea, Alejandro, Aranda, Carmen, Wu, Yinqi, Molina-Espeja, Patricia, Maté, Diana M., González-Pérez, David, Zhang, Wuyuan, Kiebist, Jan, Hofrichter, Martin, Świderek, Katarzyna, Moliner, Vicent, Sanz Aparicio, Julia, Hollmann, Frank, Gutiérrez Suárez, Ana, and Alcalde Galeote, Miguel
- Subjects
Regioselectivity ,General Medicine ,General Chemistry ,Over-Oxidation ,Protein Engineering ,Hydroxy Fatty Acids ,Unspecific Peroxygenase ,Catalysis ,Hydroxyfatty acids - Abstract
10 páginas.- 4 figuras.- 2 tablas.- 30 referencias.- Supporting information for this article is given via a link at the end of the document https://onlinelibrary.wiley.com/doi/10.1002/anie.202217372, he hydroxylation of fatty acids is an appealing reaction in synthetic chemistry, although the lack of selective catalysts hampers its industrial implementation. Here, we have engineered a highly regioselective fungal peroxygenase for the w-1 hydroxylation of fatty acids with quenched stepwise over-oxidation. One single mutation near the Phe catalytic tripod narrowed the heme cavity, promoting a dramatic shift toward sub-terminal hydroxylation with a drop in the over-oxidation activity. While crystallographic soaking experiments and molecular dynamic simulations shed light on this unique oxidation pattern, the selective biocatalyst was produced by Pichia pastoris at 0.4 g/L in a fed-batch bioreactor and used in the preparative synthesis of 1.4 g of (w-1)-hydroxytetradecanoic acid with 95% regioselectivity and 83% ee through the (S)-enantiomer.The hydroxylation of fatty acids is an appealing reaction in synthetic chemistry, although the lack of selective catalysts hampers its industrial implementation. Here, we have engineered a highly regioselective fungal peroxygenase for the w-1 hydroxylation of fatty acids with quenched stepwise over-oxidation. One single mutation near the Phe catalytic tripod narrowed the heme cavity, promoting a dramatic shift toward sub-terminal hydroxylation with a drop in the over-oxidation activity. While crystallographic soaking experiments and molecular dynamic simulations shed light on this unique oxidation pattern, the selective biocatalyst was produced by Pichia pastoris at 0.4 g/L in a fed-batch bioreactor and used in the preparative synthesis of 1.4 g of (w-1)-hydroxytetradecanoic acid with 95% regioselectivity and 83% ee through the (S)-enantiomer., This work was supported by the European Union Project grant H2020-BBI-PPP-2015-2-720297-ENZOX2, the I+D+I PID2019-106166RB-I00-OXYWAVE Spanish project funded by the Ministerio de Ciencia e Innovación/Agencia Estatal de Investigación (AEI)/doi: 10.13039/501100011033/, the PID2020-118968RB-I00 LILI project from the Spanish MCIN/AEI/10.13039/501100011033, the ‘Comunidad de Madrid’ Synergy CAM project Y2018/BIO-4738-EVOCHIMERA-CMand the PIE-CSIC projects PIE-202040E185 and PIE-201580E042. PGS thanks the Ministry of Science, Innovation and Universities (Spain) for her FPI scholarship (BES-2017-080040) and to the Ministry of Science and Innovation for her contract as part of PTQ2020-011037 project funded by MCIN/AEI/10.13039/501100011033 within the NextGenerationEU/PRTR.DGP thanks Juan de la Cierva Incorporación contract Ref nº: IJC2020-043725-I, funded by MCIN / AEI / 10.13039/501100011033, and the EUNextGenerationEU/PRTR program. The authors thank the Synchrotron Radiation Source at Alba (Barcelona, Spain) for assistance with the BL13-XALOC beamline.
- Published
- 2023
11. Oxidoreductases on their way to industrial biotransformations
- Author
-
Mehmet E. Sener, Owik Matthias Herold-Majumdar, Francisco J. Ruiz-Dueñas, Craig B. Faulds, Roland Ludwig, Victor Guallar, Sibel Kilic, Alessandra Piscitelli, Marta Tortajada, Willem J. H. van Berkel, Ana Serrano, Giovanni Sannia, Ana Gutiérrez, Henrik Lund, Morten Tovborg, María Lucas, Katrin Scheibner, Miguel Alcalde, José C. del Río, Elena Fernández-Fueyo, Susana Camarero, Ángel T. Martínez, René Ullrich, Jesper Vind, Ralf Zuhse, Jorge Rencoret, Christiane Liers, Jo-Anne Rasmussen, Eric Record, Martin Hofrichter, Dolores Linde, Cinzia Pezzella, Ib Winckelmann, Mirjana Gelo-Pujic, Frank Hollmann, Barcelona Supercomputing Center, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Novozymes A/S, Technische Universität Dresden (TUD), Jena University Hospital, Università degli Studi di Napoli Federico II, Università degli studi di Napoli Federico II, Setas Kimya Sanayi AS, Wageningen University and Research Center (WUR), Anaxomics, Barcelona Supercomputing Center - Centro Nacional de Supercomputacion (BSC - CNS), Chiracon GmBH, Universität für Bodenkultur Wien [Vienne, Autriche] (BOKU), Department of Biotechnology [Delft], Delft University of Technology (TU Delft), Biodiversité et Biotechnologie Fongiques (BBF), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM), Biopolis, Cheminova A/S, CLEA Technologies BV, Solvay, Instituto de Recursos Naturales y Agrobiología de Sevilla, Instituto de Catálisis y Petroleoquímica, European Project: 613549, European Commission, Ministerio de Economía y Competitividad (España), University of California, Technische Universität Dresden = Dresden University of Technology (TU Dresden), Wageningen University and Research [Wageningen] (WUR), Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA)-École Centrale de Marseille (ECM), Instituto de Recursos Naturales y Agrobiología de Sevilla (IRNAS), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), BIO2014-56388-R AGL2014-53730-R, H2020-BBI-PPP-2015-2-720297, DE-AC02-05CH11231, University of Naples Federico II = Università degli studi di Napoli Federico II, Universität für Bodenkultur Wien = University of Natural Resources and Life [Vienne, Autriche] (BOKU), Martínez, Angel T, Ruiz Dueñas, Francisco J, Camarero, Susana, Serrano, Ana, Linde, Dolore, Lund, Henrik, Vind, Jesper, Tovborg, Morten, Herold Majumdar, Owik M, Hofrichter, Martin, Liers, Christiane, Ullrich, René, Scheibner, Katrin, Sannia, Giovanni, Piscitelli, Alessandra, Pezzella, Cinzia, Sener, Mehmet E, Kılıç, Sibel, van Berkel, Willem J. H, Guallar, Victor, Lucas, Maria Fátima, Zuhse, Ralf, Ludwig, Roland, Hollmann, Frank, Fernández Fueyo, Elena, Record, Eric, Faulds, Craig B, Tortajada, Marta, Winckelmann, Ib, Rasmussen, Jo Anne, Gelo Pujic, Mirjana, Gutiérrez, Ana, Del Río, José C, Rencoret, Jorge, and Alcalde, Miguel
- Subjects
0301 basic medicine ,dynamique computationnelle des fluides ,oxidoréductase ,[SDV]Life Sciences [q-bio] ,Enzyme activation ,Laccases ,Lytic polysaccharide monooxygenases ,Applied Microbiology and Biotechnology ,Biochemistry ,Lignin ,chemistry.chemical_compound ,péroxydase ,Organic chemistry ,peroxyde ,production d'enzyme ,Biotransformation ,Heme peroxidases and peroxygenases ,Heme peroxidases and peroxygenase ,chemistry.chemical_classification ,Enginyeria biomèdica [Àrees temàtiques de la UPC] ,oxidase ,monooxygénase ,Directed evolution ,3. Good health ,Oxidases and dehydrogenases ,Peroxidases ,Oxigenases ,Oxidoreductases ,Oxidation-Reduction ,Biotechnology ,Dinitrocresols ,Lignocellulose biorefinery ,Biochemie ,Bioengineering ,Enzyme cascades ,Heme ,Redox ,03 medical and health sciences ,peroxides ,Oxidoreductase ,oxydase ,Biochemical computers ,Enzyme cascade ,Cellulose ,Biophysical and biochemical computational modeling ,VLAG ,Laccase ,Lytic polysaccharide monooxygenase ,Oxidases and dehydrogenase ,Rational design ,Fungi ,Monooxygenase ,Oxidoreductases--Analysis ,030104 developmental biology ,Selective oxyfunctionalization ,chemistry ,Enzims - Abstract
17 páginas.-- 13 figuras.-- 104 referencias.-- . Supplementary data associated with this article can be found in the online version, at http://dx.doi.org/10.1016/j.biotechadv.2017.06.003, Fungi produce heme-containing peroxidases and peroxygenases, flavin-containing oxidases and dehydrogenases, and different copper-containing oxidoreductases involved in the biodegradation of lignin and other recalcitrant compounds. Heme peroxidases comprise the classical ligninolytic peroxidases and the new dye-decolorizing peroxidases, while heme peroxygenases belong to a still largely unexplored superfamily of heme-thiolate proteins. Nevertheless, basidiomycete unspecific peroxygenases have the highest biotechnological interest due to their ability to catalyze a variety of regio- and stereo-selective monooxygenation reactions with H2O2 as the source of oxygen and final electron acceptor. Flavo-oxidases are involved in both lignin and cellulose decay generating H2O2 that activates peroxidases and generates hydroxyl radical. The group of copper oxidoreductases also includes other H2O2 generating enzymes - copper-radical oxidases - together with classical laccases that are the oxidoreductases with the largest number of reported applications to date. However, the recently described lytic polysaccharide monooxygenases have attracted the highest attention among copper oxidoreductases, since they are capable of oxidatively breaking down crystalline cellulose, the disintegration of which is still a major bottleneck in lignocellulose biorefineries, along with lignin degradation. Interestingly, some flavin-containing dehydrogenases also play a key role in cellulose breakdown by directly/indirectly “fueling” electrons for polysaccharide monooxygenase activation. Many of the above oxidoreductases have been engineered, combining rational and computational design with directed evolution, to attain the selectivity, catalytic efficiency and stability properties required for their industrial utilization. Indeed, using ad hoc software and current computational capabilities, it is now possible to predict substrate access to the active site in biophysical simulations, and electron transfer efficiency in biochemical simulations, reducing in orders of magnitude the time of experimental work in oxidoreductase screening and engineering. What has been set out above is illustrated by a series of remarkable oxyfunctionalization and oxidation reactions developed in the frame of an intersectorial and multidisciplinary European RTD project. The optimized reactions include enzymatic synthesis of 1-naphthol, 25-hydroxyvitamin D3, drug metabolites, furandicarboxylic acid, indigo and other dyes, and conductive polyaniline, terminal oxygenation of alkanes, biomass delignification and lignin oxidation, among others. These successful case stories demonstrate the unexploited potential of oxidoreductases in medium and large-scale biotransformations., This work has been funded by the INDOX European project (KBBE-2013-7-613549), together with the BIO2014-56388-R and AGL2014-53730-R projects of the Spanish Ministry of Economy and Competitiveness (MINECO) co-financed by FEDER funds, and the BBI JU project EnzOx2 (H2020-BBI-PPP-2015-2-720297). The work conducted by the US DOE JGI was supported by the Office of Science of the US DOE under contract number DE-AC02-05CH11231.
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.