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To investigate the effect of c-Met inhibitor cabozantinib (XL-184) in inhibiting Listeria monocytogenes (LM) from invading Caco-2 cells to reduce the cell injury.The cell invasion capacity of LM was assayed in Caco-2 cells incubated with different doses of XL-184 for different durations. Caco-2 cells incubated with XL-184 were seeded on the upper room of the transwell chamber, and the cell monolayer was exposed to LM infection followed by addition of horseradish peroxidase (HRP). The trans-epithelial electric resistance (TEER), HRP concentration and LM colony-forming unit (CFU) were measured in the cell monolayer. Fluorescent staining was used to evaluate the cell viability, and LDH release from the cells was examined to assess the changes in cell membrane permeability.XL-184 significantly decreased LM invasion rate in Caco-2 cells in a dose- and time-dependent manner (P=0.000), and this effect was enhanced by co-incubation of the cells with ampicillin (P0.05). In the cell membrane permeability assay in the monolayer cells, XL-184 markedly inhibited LM-induced reduction of TEER (P0.05) and significantly suppressed LM-induced enhancement of cell membrane permeability shown by reduced HRP concentration and LM count in the lower chamber (P=0.000). The cells infected with LM showed significantly lowered cell viability, which was rescued by XL-184 (P0.01); XL-184 also dose-dependently reduced LDH release from the cells (P0.05).XL-184 can suppress LM invasion in Caco-2 cells to reduce the cell injury, suggesting its value as a promising candidate agent for prevention and treatment of LM infections.

In the framework of quantum defect theory, we study super-excited states of F2 molecules which can dissociate into F+(3P2,1,0) and F−(1S0) ion-pair. Based on our calculation, we present a vibrational resolved assignment of the high precision photofragment yield spectra for F− from the F2 ion-pair production.

The steady-state current (dc) characterization of high temperature superconducting (HTS) coils is a key role of its performance assessing. Due to the orientation dependence of the critical current density, Jc(B,θ), the magnetic field distribution of a given HTS coils depends on the shape of coils as well as the consistent tape applied. In the present paper, the dc characterizations of two HTS coils with same turns but different architectures are modeled by using the 3D finite element method. It is revealed that the engineering critical currents of both coils are subject to the end parts of coils. As the dc current is applied, the central magnetic field emerged is similar for both of them. As the dc current increases, the generated voltage for Coil A with an high aspect ratio of height to outer radius, is much lower than that of Coil B with a squashed shape, implying that Coil A gives rise to a higher toleration for applied current, and then a better performance for potential magnet.

In the framework of quantum defect theory, the photofragment yield spectra for F- from F2 ion-pair production have been studied. The multiple-scattering self-consistent field method and the theoretical time-dependent wave-packet dynamics method are employed. Based on our calculated results, the vibrational resolved assignment and the calculated relative intensity are provided.