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Additional file 1. Material in qRT-PCR, western and field application. Quantitative detection of NbHsp70 gene and TMV-CP gene. Table S1. Primer pairs used to detect gene RNA accumulation. Quantitative detection of Hsp70 protein and TMV-CP protein. Methods of disease grading. Figure S1. The standard curve of quercetin. Figure S2. The zeta potential (A), particle size (B), PDI C and quercetin concentration D of H-TQ-NP solutions with different HACC concentrations; bars with the same letters show no significant differences (LSD test, p

Phytophthora parasitica is an important oomycete that causes disease in a variety of plants, dimethomorph fungicides being specific for oomycetes. The aim of this study was to use RNA-seq to rapidly discover the mechanism by which dimethomorph acts in the treatment of P. parasitica. We found that the expression of 832 genes changed significantly after the dimethomorph treatment, including 365 up-regulated genes and 467 down-regulated genes. According to the Gene Ontology (GO) enrichment analysis, pathway enrichment and verification test results, the following conclusions are obtained: (i) the treatment of P. parasitica with dimethomorph causes changes in the expression levels of genes associated with the cell wall and cell wall synthesis; (ii) dimethomorph treatment results in reduced permeability of the cell membrane and changes in the expression of certain transport-related proteins; (iii) dimethomorph treatment increased reactive oxygen species and reduced the expression of genes related to the control of oxidative stress.