1. Complete genomic sequence and phylogenetic relatedness of hepatitis B virus isolates in Cambodia
- Author
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Amadou A. Sall, Tran Thien Tuan Huy, Kenji Abe, Jean Marc Reynes, National Institute of Infectious Diseases [Tokyo], Unité de Virologie / Virology Unit [Phnom Penh], Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Institut Pasteur de Dakar, Réseau International des Instituts Pasteur (RIIP), Institut Pasteur de Madagascar, and This study was supported in part by grants-in-aid for Science Research of the Ministry of Education, Culture, Sports, Science and Technology of Japan and the Ministry of Health, Labor and Welfare of Japan.
- Subjects
Male ,MESH: Protein Precursors ,Phylogenetic relatedness ,MESH: Amino Acid Sequence ,MESH: Base Sequence ,medicine.disease_cause ,MESH: Genotype ,0302 clinical medicine ,Medical microbiology ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Genotype ,MESH: Phylogeny ,Phylogeny ,Genetics ,0303 health sciences ,Molecular Epidemiology ,Phylogenetic tree ,virus diseases ,Endemic area ,General Medicine ,3. Good health ,MESH: Hepatitis B virus ,HBV genotypes ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,030211 gastroenterology & hepatology ,MESH: Genome, Viral ,Cambodia ,Adult ,medicine.medical_specialty ,Hepatitis B virus ,MESH: Hepatitis B, Chronic ,Molecular Sequence Data ,MESH: Sequence Alignment ,Genome, Viral ,Biology ,HBV Asian strain ,03 medical and health sciences ,Hepatitis B, Chronic ,Virology ,medicine ,MESH: Molecular Epidemiology ,Humans ,Hepatitis B virus (HBV) ,Amino Acid Sequence ,Protein Precursors ,Molecular Biology ,Gene ,030304 developmental biology ,Sequence (medicine) ,MESH: Molecular Sequence Data ,MESH: Humans ,Hepatitis B Surface Antigens ,Base Sequence ,MESH: Cambodia ,MESH: Adult ,MESH: Male ,digestive system diseases ,MESH: Hepatitis B Surface Antigens ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,Sequence Alignment ,HBV genome - Abstract
International audience; Although it is known that Cambodia is one of the high endemic area of hepatitis B virus (HBV) infection, molecular characterization of HBV circulating in this country has not been reported. In this study, pre-S gene of HBV from 12 Cambodian patients was sequenced. Phylo-genetic analysis based on the pre-S gene sequence revealed that 8 out of 12 isolates (66.7%) belonged to HBV/C1 and remaining four (33.3%) were HBV/B4. Furthermore, complete genomic sequences were also determined for three Cambodian HBV isolates. They all comprised of 3,215 bp long and two of them belonged to subgenotype B4, which had recombination event with genotype C in the precore/core gene confirmed by SimPlot and BootScanning analyses. Our results showed that both HBV strains belonged to subgenotypes B4 and C1, which are circulating in this country. This is the first report on molecular characterization of the HBV prevalent in Cambodia.
- Published
- 2007
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