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To produce high concentrations of hyperoside from quercetin using recombinant Escherichia coli with in situ regeneration of UDP-galactose.Sucrose synthase from Glycine max (GmSUS) was co-expressed with UDP-glucose epimerase from E. coli (GalE) in E. coli for regenerating UDP-galactose from UDP and sucrose. Glycosyltransferase from Petunia hybrida (PhUGT) was introduced to synthesize hyperoside from quercetin through the regeneration system of UDP-galactose. Co-expressing with molecular chaperones GroEL/ES successfully enhanced the catalytic efficiency of the recombinant strain, which assisted the soluble expression of PhUGT. By using a fed-batch approach, the production of hyperoside reached 863.7 mg LThe method described herein for hyperoside production can be widely applied for the synthesis of isorhamnetin-3-O-galactoside, kaempferol-3-O-galactoside and other flavonoids.

We study the relations between low-energy constants (LECs) in the chiral Lagrangians with $\Delta(1232)$ and those in the quark-level description model up to the third chiral order. Ten structure correspondences are involved in getting the relations. This situation is more complicated than the spin-1/2 baryon case. The obtained results may help to further investigations involving the $\Delta(1232)$ baryons., Comment: Version accepted by Eur. Phys. J. C. 10 pages, 2 figures, 4 tables

Materials with superconductivity and a nontrivial band structure near the Fermi level are promising candidates in realizing topological superconductivity. Using first-principles calculations, we systematically investigated the stability, mechanical properties, superconductivity, electronic structures, and topological states of hexagonal TaC and NbC. The results show that they are stable and have excellent mechanical properties. We predicted that these two carbides are strong electron-phonon coupling superconductors with superconducting transition temperatures of 14.8 and 17.1 K, respectively. Strong coupling is mainly dominated by in-plane Ta/Nb atomic vibrations and in-plane Ta/Nb-d

Studies showed that the increase of myeloid‐derived suppressor cells (MDSCs) in tumour microenvironment is closely related to the resistant treatment and poor prognosis of metastatic breast cancer. However, the effect of tumour‐derived exosomes on MDSCs and its mechanism are not clear. Here, we reported that breast cancer cells (4T1)‐secreted exosomes (BCC‐Ex) were able to differentiate bone marrow cells into MDSCs and significantly inhibited the proliferation of T lymphocytes to provide an immunosuppressive microenvironment for cancer cells in vivo and in vitro. The number of MDSCs in bone marrow and spleen of 4T1 tumour‐bearing mice and BCC‐Ex infused mice was significantly higher than that of normal mice, whereas the number of T lymphocytes in spleen was significantly decreased. In addition, BCC‐Ex markedly promoted the differentiation of MDSCs from bone marrow cells or bone marrow cells derived macrophages, seen as the increased expressions of MDSCs‐related functional proteins Arginase‐1 (Arg‐1) and inducible nitric oxide synthase (iNOS). Furthermore, BCC‐Ex significantly down‐regulated the expressions of chemokine receptor CXCR4 and markedly up‐regulated the levels of inflammatory cytokines IL‐6 and IL‐10 in bone marrow cells and macrophages and remarkably inhibited the division and proliferation of T cells. Importantly, CXCR4 agonist, CXCL12, could reverse the function of BCC‐Ex, indicating that BCC‐Ex‐induced MDSCs might be dependent on the down‐regulation of CXCR4. Western blot showed that BCC‐Ex significantly promoted the phosphorylation of STAT3 in bone marrow cells, resulting in the inhibitions of the proliferation and apoptosis of bone marrow cells, and the aggravation of the differentiation of bone marrow cells into MDSCs.

To enhance biotin production in Escherichia coli by engineering a heterologous biotin synthetic pathway. Biotin operon genes from Pseudomonas putida, which consisted of a bioBFHCD cluster and a bioA gene, was engineered into Escherichia coli for biotin production. The introduction of bioW gene from Bacillus subtilis, encoding pimeloyl-CoA synthetase and sam2 gene from Saccharomyces cerevisiae, encoding S-adenosyl-L-methionine (SAM) synthetase contributed to the heterologous production of biotin in recombinant E. coli. Furthermore, biotin production was efficiently enhanced by optimization of the fermentation compositions, especially pimelic acid and L-methionine, the precursor related to the pimeloyl-CoA and SAM synthesis, respectively. The combination of overexpression of the heterologous biotin operon genes and enhanced supply of key intermediate pimeloyl-CoA and SAM increased biotin production in E. coli by more than 121-fold. With bioprocess engineering efforts, biotin was produced at a final titer of 92.6 mg/L in a shake flask and 208.7 mg/L in a fed-batch fermenter. Through introduction of heterologous biotin synthetic pathway, increasing the supply of precursor pimeloyl-CoA and cofactor SAM can significantly enhance biotin production in E. coli.

In this article, a new power switch open-circuit fault diagnosis approach is proposed for the six-phase fault-tolerant permanent magnet synchronous motor (FTPMSM) system based on average current Park's vector (ACPV), which can detect the fault power switch for the FTPMSM system in both normal and phase open-circuit/short-circuit fault-tolerant operation conditions. To detect the power switch open-circuit fault, the fault detection approach based on the moduli of the ACPV in two orthogonal subspaces is first proposed for the six-phase FTPMSM system even in phase open-circuit and short-circuit fault-tolerant operation. Then, the ACPV-based fault location approach is proposed, which can identify the fault power switch according to the sign symbol of the ACPV. The resulting fault diagnosis approach has a simple structure and low computation burden, which can detect the power switch open-circuit fault online for the FTPMSM system in both normal and fault-tolerant operation conditions. Finally, the effectiveness of the proposed fault diagnosis approach is demonstrated by a six-phase FTPMSM system experimental platform.

Asia is the largest and most populous continent of Earth with an overall population of 446.27 crores. Housing this population drives various challenges in different aspects, in which providing healthy and nutritious food to each and every individual is most challenging. Unfortunately, because of certain production issues, farmers are not getting the desired throughput, and in some critical cases, situations lead to suicide. On the other hand, proper storage and distribution of these harvests are other challenges. Various models and IoT (internet of things)-based approaches have been presented and are available for implementation, but a dedicated layout combining the digitization of all the essential steps, starting from preparation of soil for agriculture to the making food available for every needy person, is still missing. In the paper, various related models have been studied, and an integral model is presented where integration of IoT is carried out with agriculture and with the food storage and distribution system.

The production mechanism of multiquark exotic hadrons in high energy multiproduction processes lies in the structure of the relevant exotic hadrons as well as in some important aspects of high energy scattering, such as multi-parton interactions, underlying events, etc. At mass pole around 2900 MeV, a family of open charm tetraquarks, Tcss and Tcs¯s, are observed in B decay. They are also suitable for study in multiproduction processes to obtain more information on their structure. If these resonances are produced as compact four-quark states, one can predict the production properties based on the similarities in their production mechanism to those of Ξc, Σc, and Λc. Physics implies that the colour and baryon number fluctuations of the preconfinement system in high energy scattering can enhance both the baryon and four-quark state production rates via ‘diquark fragmentation’. We calculate the production properties of the tetraquark family Tcss and Tcs¯s at LHC energy for the forthcoming LHC measurements.

The Transition Edge Sensor is extremely sensitive to the change of temperature, combined with the high-Z metal of a certain thickness, it can realize the high energy resolution measurement of particles such as X-rays. X-rays with energies below 10 keV have very weak penetrating ability, so only a few microns thick of gold or bismuth can obtain quantum efficiency higher than 70\%. Therefore, the entire structure of the TES X-ray detector in this energy range can be realized in the microfabrication process. However, for X-rays or gamma rays from 10 keV to 200 keV, sub-millimeter absorber layers are required, which cannot be realized by microfabrication process. This paper first briefly introduces a set of TES X-ray detectors and their auxiliary systems built by ShanghaiTech University, then focus on the introduction of the TES $γ$-ray detector, with absorber based on an sub-millimeter lead-tin alloy sphere. The detector has a quantum efficiency above 70\% near 100 keV, and an energy resolution of about 161.5eV@59.5keV., 13 pages, 12 figures

The quark model symmetry can be adopted to establish relations between the low-energy constants (LECs) in the baryon chiral perturbation theory ($\chi PT$) if one assumes that a baryon-baryon-meson coupling is described equivalently by a quark-quark-meson coupling at the quark level. Through the correspondence between the $SU(2)$ description and the $SU(3)$ description for the same coupling vertex at the quark level, we find some relations between the LECs in $SU(2)_{\chi PT}$ and $SU(3)_{\chi PT}$ up to the third chiral order. The $SU(3)_{\chi PT}$ LEC relations at the same order are also obtained. The numerical analysis roughly supports these relations. In the situation that the available experimental data are not enough, one may employ such constraints to reduce the number of LECs., Comment: Version to appear in PRD. 16 pages, 2 figures, 7 tables

Enzymatic oxidative dehydrogenation of biomass-derived l -lactate in the presence of O2 is regarded as a green alternative for pyruvate production, but the process also results in H2O2 accumulation. To remove the effect of H2O2 on pyruvate production, various potential catalases from different species were screened for their efficiency in H2O2 degradation. Then, an in vitro cascade biocatalysis was designed using lactate oxidase from Aerococcus viridans (AvLOX) and catalase from Ureibacillus thermosphaericus (UtCAT). The in situ removal of H2O2 by UtCAT significantly improved the yield and productivity of pyruvate. To simplify the technological processes and reduce production costs, a whole cell biocatalyst without the addition of catalase for the production of pyruvate was established by coexpressing AvLOX and UtCAT in Escherichia coli. By applying suitable coexpression strategies and changing the ribosome binding site (RBS) strengths, the optimal ratio of AvLOX:UtCAT(12.6:4127.3) in E. coli-AvLOX-(rbs3)UtCAT was finally achieved. Under the optimized transformation conditions, pyruvate was produced at a titer of 59.9 g/L and a yield of 90.8% in a substrate fed-batch process, promising an alternative route for the green production of pyruvate.

This study proposes an optimisation approach of high torque density with guaranteed anti-short-circuit performance for the dual redundant permanent magnet synchronous motor (PMSM) in an aerospace application. The 10-pole/12-slot dual redundant PMSM with concentrated single-layer windings is firstly proposed, which can restrain the short-circuit current by the slot design to increase the phase inductance. Since the anti-short-circuit design may result in the torque density degradation of the dual redundant PMSM, the influence of the slot parameters on the torque performance of the dual redundant PMSM is thoroughly investigated, including the electromagnetic torque, braking torque, and no-load torque. Furthermore, the genetic algorithm-based optimisation method is proposed to maximise the torque density of the dual redundant PMSM, while the anti-short-circuit performance can also be guaranteed. Simulation and experimental results are conducted to verify the effectiveness of the proposed optimisation method.

We suggest to study the production mechanism and some details of the production properties to probe the structure of the DDπ resonance Tcc recently observed by the LHCb Collaboration. If the resonance is produced as a four-quark state, one can find the corresponding signals via measurements on the production properties which are the same as those of Ξcc, i.e., the similarity of Tcc and Ξcc in the kinematics spectra, in the baryon number enhancement, and in the string effect, etc. The color connection of the preconfinement cluster in which the cc diquark pair hadronize to the Tcc or Ξcc plays the key role for their similarity. Moreover, the production cross section of Tcc in various rapidity regions and the production ratio between Tcc to a different isospin state Tcc′ (if it exists) are also important and clear tests. On the other hand, if Tcc is produced as a hadron molecule, the measurement on the momentum correlation of DD* can be a key judgement, since it determines the production ratio. From the present available information, the calculated cross section with nonrelativistic hadron molecule model is about an order of magnitude lower than the experimental measurement.

The tiny neutrino masses can be explained naturally by extending the standard model with right-handed Majorana neutrinos in a low-scale seesaw mechanism, and the CP violation effect induced by the Majorana phase is interesting and worth studying at colliders. In this paper, we investigate the prospects for measuring CP violation in top quark pair production and rare decay via Majorana neutrinos at the LHC. The CP asymmetry stems from the significant interference of contributions from two different Majorana neutrinos and can be appreciable when the two neutrino masses are nearly degenerate. It is found that in the Majorana neutrino mass range of 10 GeV

Ergosterol, a terpenoid compound produced by fungi, is an economically important metabolite serving as the direct precursor of steroid drugs. Herein, ergsosterol biosynthetic pathway modification combined with storage capacity enhancement was proposed to synergistically improve the production of ergosterol in Saccharomyces cerevisiae. S. cerevisiae strain S1 accumulated the highest amount of ergosterol [7.8 mg/g dry cell weight (DCW)] among the wild-type yeast strains tested and was first selected as the host for subsequent metabolic engineering studies. Then, the push and pull of ergosterol biosynthesis were engineered to increase the metabolic flux, overexpression of the sterol acyltransferase gene ARE2 increased ergosterol content to 10 mg/g DCW and additional overexpression of a global regulatory factor allele (UPC2-1) increased the ergosterol content to 16.7 mg/g DCW. Furthermore, considering the hydrophobicity sterol esters and accumulation in lipid droplets, the fatty acid biosynthetic pathway was enhanced to expand the storage pool for ergosterol. Overexpression of ACC1 coding for the acetyl-CoA carboxylase increased ergosterol content from 16.7 to 20.7 mg/g DCW. To address growth inhibition resulted from premature accumulation of ergosterol, auto-inducible promoters were employed to dynamically control the expression of ARE2, UPC2-1, and ACC1. Consequently, better cell growth led to an increase of ergosterol content to 40.6 mg/g DCW, which is 4.2-fold higher than that of the starting strain. Finally, a two-stage feeding strategy was employed for high-density cell fermentation, with an ergosterol yield of 2986.7 mg/L and content of 29.5 mg/g DCW. This study provided an effective approach for the production of ergosterol and other related terpenoid molecules.

To enhance biotin production in Escherichia coli by engineering a heterologous biotin synthetic pathway.Biotin operon genes from Pseudomonas putida, which consisted of a bioBFHCD cluster and a bioA gene, was engineered into Escherichia coli for biotin production. The introduction of bioW gene from Bacillus subtilis, encoding pimeloyl-CoA synthetase and sam2 gene from Saccharomyces cerevisiae, encoding S-adenosyl-L-methionine (SAM) synthetase contributed to the heterologous production of biotin in recombinant E. coli. Furthermore, biotin production was efficiently enhanced by optimization of the fermentation compositions, especially pimelic acid and L-methionine, the precursor related to the pimeloyl-CoA and SAM synthesis, respectively. The combination of overexpression of the heterologous biotin operon genes and enhanced supply of key intermediate pimeloyl-CoA and SAM increased biotin production in E. coli by more than 121-fold. With bioprocess engineering efforts, biotin was produced at a final titer of 92.6 mg/L in a shake flask and 208.7 mg/L in a fed-batch fermenter.Through introduction of heterologous biotin synthetic pathway, increasing the supply of precursor pimeloyl-CoA and cofactor SAM can significantly enhance biotin production in E. coli.

Stem cells including embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs) and adult stem cells (ASCs) are able to repair/replace damaged or degenerative tissues and improve functional recovery in experimental model and clinical trials. However, there are still many limitations and unresolved problems regarding stem cell therapy in terms of ethical barriers, immune rejection, tumorigenicity, and cell sources. By reviewing recent literatures and our related works, human amnion-derived stem cells (hADSCs) including human amniotic mesenchymal stem cells (hAMSCs) and human amniotic epithelial stem cells (hAESCs) have shown considerable advantages over other stem cells. In this review, we first described the biological characteristics and advantages of hADSCs, especially for their high pluripotency and immunomodulatory effects. Then, we summarized the therapeutic applications and recent progresses of hADSCs in treating various diseases for preclinical research and clinical trials. In addition, the possible mechanisms and the challenges of hADSCs applications have been also discussed. Finally, we highlighted the properties of hADSCs as a promising source of stem cells for cell therapy and regenerative medicine and pointed out the perspectives for the directions of hADSCs applications clinically.

A completely relativistic quark model in the Bethe-Salpeter framework is employed to calculate the exclusive production ratio of the neutral to charged kaon pair in ${e}^{+}{e}^{\ensuremath{-}}$ annihilation continuum region below the $J/\mathrm{\ensuremath{\Psi}}$ mass. The valence quark charge plays the key role. The cancellation of the diagrams for the same charge case (${K}_{S}+{K}_{L}$) and the noncancellation of the diagrams for the different charge case (${K}^{\ensuremath{-}}+{K}^{+}$) lead to the ratio as ${({m}_{s}\ensuremath{-}{m}_{d})}^{2}/{M}_{kaon}^{2}\ensuremath{\approx}1/10$.

Right-handed (RH) Majorana neutrinos play a crucial role in understanding the origin of neutrino mass, the nature of dark matter and the mechanism of matter-antimatter asymmetry. In this work, we investigate the observability of heavy Majorana neutrino through the top quark neutrinoless double beta decay process $t \to b \ell^+ \ell^+ j j$ at hadron colliders. By performing detector level simulation, we demonstrate that our method can give stronger limits on the light-heavy neutrino mixing parameters $|V_{eN, \mu N}|$ in the mass range of 15 GeV $< m_N, Comment: 6 pages, discussions added, version published in PRD (rapid communication)

We calculate the masses of the $QQ\bar{q}\bar{q}$ ($Q=c,b$; $q=u,d,s$) tetraquark states with the aid of heavy diquark-antiquark symmetry (HDAS) and the chromomagnetic interaction (CMI) model. The masses of the highest-spin ($J=2$) tetraquarks that have only the $(QQ)_{\bar{3}_c}(\bar{q}\bar{q})_{3_c}$ color structure are related with those of conventional hadrons using HDAS. Thereafter, the masses of their partner states are determined with the mass splittings in the CMI model. Our numerical results reveal that: (i) the lightest $cc\bar{n}\bar{n}$ ($n=u,d$) is an $I(J^P)=0(1^+)$ state around 3929 MeV (53 MeV above the $DD^*$ threshold) and none of the double-charm tetraquarks are stable; (ii) the stable double-bottom tetraquarks are the lowest $0(1^+)$ $bb\bar{n}\bar{n}$ around 10488 MeV ($\approx116$ MeV below the $BB^*$ threshold) and the lowest $1/2(1^+)$ $bb\bar{n}\bar{s}$ around 10671 MeV ($\approx20$ MeV below the $BB_s^*/B_sB^*$ threshold); and (iii) the two lowest $bc\bar{n}\bar{n}$ tetraquarks, namely the lowest $0(0^+)$ around 7167 MeV and the lowest $0(1^+)$ around 7223 MeV, are near-threshold states. Moreover, we discuss the constraints on the masses of double-heavy hadrons. Specifically, for the lowest nonstrange tetraquarks, we obtain $T_{cc}, 19 pages, 4 figures, and 11 tables

To strengthen NADH regeneration in the biosynthesis of l-2-aminobutyric acid (l-ABA). l-Threonine deaminase (l-TD) from Escherichia coli K12 was modified by directed evolution and rational design to improve its endurance to heat treatment. The half-life of mutant G323D/F510L/T344A at 42 °C increased from 10 to 210 min, a 20-fold increase compared to the wild-type l-TD, and the temperature at which the activity of the enzyme decreased by 50% in 15 min increased from 39 to 53 °C. The mutant together with thermostable l-leucine dehydrogenase from Bacillus sphaericus DSM730 and formate dehydrogenase from Candida boidinii constituted a one-pot system for l-ABA biosynthesis. Employing preheat treatment in the one-pot system, the biosynthesis of l-ABA and total turnover number of NAD+/NADH were 0.993 M and 16,469, in contrast to 0.635 M and 10,531 with wild-type l-TD, respectively. By using the engineered l-TD during endured preheat treatment, the one-pot system has achieved a higher productivity of l-ABA and total turnover number of coenzyme.

The Higgs-top coupling plays a central role in the hierarchy problem and the vacuum stability of the Standard Model (SM). We propose a central-edge asymmetry ( A C E ) to probe the CP violating Higgs-top coupling in dileptonic channel of t t ¯ h ( → b b ¯ ) production at the LHC. We demonstrate that the CP-violating Higgs-top coupling can affect the central-edge asymmetry through distorting Δ y l + l − distribution because of the contribution of new top charge asymmetric term. Since Δ y l + l − distribution is frame-independent and has a good discrimination even in boosted regime, we use the jet substructure technique to enhance the observability of the dileptonic channel of t t ¯ h production. We find that (1) the significance of dileptonic channel of t t ¯ h production can reach 5 σ for CP phase ξ = 0 , π / 4 , π / 2 when the luminosity L = 795 , 993 , 1276 fb − 1 at 14 TeV LHC. (2) the central-edge asymmetry A C E show a good discrimination power of CP phase of t t ¯ h interaction, which are − 40.26 % , − 26.60 % , − 9.47 % for ξ = 0 , π / 4 , π / 2 respectively and are hardly affected by the event selections. Besides, by performing the binned- χ 2 analysis of Δ y l + l − distribution, we find that the scalar and pseudo-scalar interactions can be distinguished at 95% C.L. level at 14 TeV HL-LHC.

The type III secretion system (T3SS) in many Gram-negative bacterial pathogens is regarded as the most critical virulence determinant and an attractive target for novel anti-virulence drugs. In this study, we constructed a T3SS secretion reporter containing the β-lactamase gene fused with a signal peptide sequence of the T3SS effector gene, and established a high-throughput screening system for T3SS inhibitors in the plant pathogenic bacterium Acidovorax citrulli. From a library of 12,000 chemical compounds, we identified a series of benzyloxy carbonimidoyl dicyanide (BCD) derivatives that effectively blocked T3SS-dependent β-lactamase secretion. Substitution of halogens or nitro groups at the para-position on the benzene ring contributed to an increased inhibitory activity. One representative compound, BCD03 (3,4-dichloro-benzyloxy carbonimidoyl dicyanide), dramatically reduced pathogenicity of A. citrulli on melon seedlings, and attenuated hypersensitive responses in the non-host Nicotiana tabacum caused by pathogenic bacteria A. citrulli, Xanthomonas oryzae pv. oryzae and Pseudomonas syringae pv. tomato at sub-MIC concentrations. Western blotting assay further confirmed that BCD03 inhibited effector secretion from the above bacteria via T3SS in the liquid medium. Taken together, our data suggest that BCD derivatives act as novel inhibitors of T3SS in multiple plant bacterial pathogens.

In an extended effective operator framework of isospin violating interactions with light mediators, we investigate the compatibility of the candidate signal of the CDMS-II-Si with the latest constraints from DarkSide-50 and XENON-1T, etc. We show that the constraints from DarkSide-50 which utilizes Argon as the target is complementary to that from XENON-1T which utilizes Xenon. Combining the results of the two experiments, we find that for isospin violating interaction with light mediator there is no parameter space which can be compatible with the positive signals from CDMS-II-Si. As a concrete example of this framework, we investigate the dark photon model in detail. We obtain the combined limits on the dark matter mass $m_{\chi}$, the dark photon mass $m_{A'}$, and the kinetic mixing parameter $\varepsilon$ in the dark photon model. The DarkSide-50 gives more stringent upper limits in the region of mediator mass from 0.001 to 1 GeV, for $m_{\chi} \lesssim 6$ GeV in the ($m_{A'}$,$\varepsilon$) plane, and more stringent constraints for $m_{\chi}\lesssim 8$ GeV and $\varepsilon \thicksim 10^{-8}$ in the ($m_{\chi}$,$m_{A'}$) plane., Comment: 22 pages, 5 figures

We discuss the mass splittings for the $S$-wave triply heavy pentaquark states with the $QQQq\bar{q}$ $(Q=b,c;q=u,d,s)$ configuration which is a mirror structure of $Q\bar{Q}qqq$. The latter configuration is related with the nature of $P_c(4380)$ observed by the LHCb Collaboration. The considered pentaquark masses are roughly estimated with a simple method. One finds that such states are probably not narrow even if they do exist. This leaves room for molecule interpretation for a state around the low-lying threshold of a doubly heavy baryon and a heavy-light meson, e.g. $\Xi_{cc}D$, if it were observed. As a by product, we conjecture that upper limits for the masses of the conventional triply heavy baryons can be determined by the masses of the conventional doubly heavy baryons.

Virus-like particles (VLPs) are potential containers for delivery of therapeutic agents at the nanoscale. In this study, the capsid protein of Infectious hypodermal and hematopoietic necrosis virus (IHHNV) was expressed in a baculovirus insect cell system. The 37-kDa recombinant protein containing the hexahistidine residues (His Tag) at N-terminal was purified using immobilized metal affinity chromatography (IMAC) and assembled into VLPs with a diameter of 23 ± 3 nm analyzed by transmission electron microscopy. We also verified that disassembly/reassembly of IHHNV-VLPs was controlled in the presence and absence of DTT. The efficiency of IHHNV-VLPs to encapsulate plasmid DNA was about 48.2%, and the VLPs encapsulating the pcDNA3.1(+)-EGFP plasmid DNA could recognize the primary shrimp hemocytes and deliver the loaded plasmid into cells by detection of expressed enhanced green fluorescent protein (EGFP). These results implied that the IHHNV-VLPs might be a good candidate for packaging and delivery of expressible plasmid DNA, and may produce an antiviral product in shrimp cells for gene therapy.

In this paper, we design and investigate the six-phase in-wheel fault-tolerant surface-mounted permanent magnet motor (SPM) and interior permanent synchronous motor (IPM) for electric vehicles (EVs) application. Firstly, the stator of the motor is designed based on the anti-short-circuit method and the main design parameters of both motors are listed. Then the electromagnetic performance and the motor loss of both motors are analyzed by finite-element analysis (FEA). In addition, the thermal analysis is conducted by utilizing Motor-CAD, which shows that both SPM and IPM encounter with magnet overheating problems. Therefore, the magnet segmentation basing on the skin depths calculation is adopted to reduce the eddy-current loss. Meanwhile, the thermal performance of both motors with non-segmented and segmented magnets under different working conditions are presented. Finally, the optimized model is achieved through the comparison between the two types of fault-tolerant permanent magnet synchronous motors (FTPMSM).

We reexamine the B → π l + l − ( l = e , μ , τ ) form factors, f + B → π ( q 2 ) , f 0 B → π ( q 2 ) and f T B → π ( q 2 ) , in the entire region of the momentum transfer squared q 2 , by taking advantage of the complementarity between lattice QCD (LQCD) simulation and light cone sum rule approach (LCSR), and analyticity of the form factors. A LCSR calculation with a chiral current correlator, which could avoid pollution by twist-3 components, is performed at twist-2 next-to-leading order (NLO) accuracy, to determinate the form factor shapes in the small and intermediate q 2 region. Further, fitting simultaneously the LCSR results for these form factors and the related LQCD ones (available or based on a SU F ( 3 ) symmetry breaking ansatz) to a Bourrely–Caprini–Lellouch (BCL) parametrization, we get a global understanding of their q 2 behaviors. Our findings turn out to be consistent with the recent study by Ali, Parkhomenko and Rusov, and the resulting observation for the vector form factor provides support for the existing LQCD as well as LCSR predictions extrapolated to the entire kinematically accessible region.

Dark sector may couple to the Standard Model via one or more mediator particles. We discuss two types of mediators: the dark photon $A^{\prime}$ and the dark scalar mediator $\phi$. The total cross-sections and various differential distributions of the processes $e^{+} e^{-} \rightarrow q \bar{q} A^{\prime}$ and $e^{+} e^{-} \rightarrow q \bar{q} \phi$ ($q=u,~d,~c,~s$ and $b$ quarks) are discussed. We focus on the study of the invisible $A^{\prime}$ due to the cleaner background at future $e^{+} e^{-}$ colliders. It is found that the kinematic distributions of the two-jet system could be used to identify (or exclude) the dark photon and the dark scalar mediator, as well as to distinguish between them. We further study the possibility of a search for dark photons at a future CEPC experiment with $\sqrt{s}=$ 91.2 GeV and 240 GeV. With CEPC running at $\sqrt{s}=$ 91.2 GeV, it would be possible to perform a decisive measurement of the dark photon (20 GeV $< m_{A^{\prime}}, Comment: 17 pages, 10 figures, to be published in Chinese Physics C

To strengthen NADH regeneration in the biosynthesis of L-2-aminobutyric acid (L-ABA).L-Threonine deaminase (L-TD) from Escherichia coli K12 was modified by directed evolution and rational design to improve its endurance to heat treatment. The half-life of mutant G323D/F510L/T344A at 42 °C increased from 10 to 210 min, a 20-fold increase compared to the wild-type L-TD, and the temperature at which the activity of the enzyme decreased by 50% in 15 min increased from 39 to 53 °C. The mutant together with thermostable L-leucine dehydrogenase from Bacillus sphaericus DSM730 and formate dehydrogenase from Candida boidinii constituted a one-pot system for L-ABA biosynthesis. Employing preheat treatment in the one-pot system, the biosynthesis of L-ABA and total turnover number of NADBy using the engineered L-TD during endured preheat treatment, the one-pot system has achieved a higher productivity of L-ABA and total turnover number of coenzyme.

The Principle of Maximum Conformality (PMC) provides a systematic way to eliminate the renormalization scheme and renormalization scale uncertainties for high-energy processes. We have observed that by applying PMC scale-setting, one obtains comprehensive and self-consistent pQCD predictions for the top-quark pair total cross-section and the top-quark pair forward-backward asymmetry in agreement with the measurements at the Tevatron and LHC. As a step forward, in the present paper, we determine the top-quark pole mass via a detailed comparison of the top-quark pair cross-section with the measurements at the Tevatron and LHC. The results for the top-quark pole mass are $m_t=174.6^{+3.1}_{-3.2}$ GeV for the Tevatron with $\sqrt{S}=1.96$ TeV, $m_t=173.7\pm1.5$ GeV and $174.2\pm1.7$ GeV for the LHC with $\sqrt{S} = 7$ TeV and $8$ TeV, respectively. Those predictions agree with the average, $173.34\pm0.76$ GeV, obtained from various collaborations via direct measurements. The consistency of the pQCD predictions using the PMC with all of the collider measurements at different energies provides an important verification of QCD., Comment: 10 pages, 6 figures. Revised version to be published in Eur.Phys.J.C

The direct observation of the Yukawa interaction related to Standard Model Higgs and the bottom quark is obtained from the $h\to b\bar{b}$ process recently. The b-tagging becomes an important tool to search for new heavy resonance production at LHC. We use the b-tagging method to investigate the neutral Higgs production in association with a bottom quark pair, and perform the detector simulation for the signal process with the Higgs decaying into $b\bar{b}$ or $\tau^+\tau^-$ together with the dominant backgrounds. Our results show that $b\bar{b}b\bar{b}$ and $b\bar{b}\tau^+\tau^-$ processes will play an important role to search for the exotic heavy Higgs production at LHC and future hadron colliders., Comment: 21 pages, 12 figures

Aluminum electrolytic cell voltage signal is the most important real-time data in the process of aluminum electrolysis. Under normal circumstances, the traditional LMD decomposition of the original voltage signal containing noise in the aluminum electrolytic cell will produce a large error and affect the diagnosis of the failure of the aluminum electrolytic cell. In this paper, an improved LMD decomposition method is used to decompose the cell voltage signal based on wavelet packet denoising. At the same time, according to the energy analysis, the extracted features are classified. The accuracy and reliability of feature extraction can be improved, and the faults existing in the aluminum electrolytic cell can be accurately diagnosed.

We study one possible production mechanism for hidden charm pentaquark in e+e- collision, where it is produced via a color-octet c \bar c pair fragmentation. The pentaquark production at B factory energy is dominated by e+e- to c \bar c g to Pc + X. At Z^0 pole, for the pentaquark production, there are several partonic processes playing significant role. Our results show that it is possible to search for the direct pentaquark production signal at e+e- colliders, which is important to understand the properties of pentaquark., Comment: 8 pages, 4 figures, 2 tables