1. Three-dimensional wide-field fluorescence microscopy for transcranial mapping of cortical microcirculation
- Author
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Zhou, Quanyu, Chen, Zhenyue, Liu, Yu-Hang, El Amki, Mohamad, Glück, Chaim, Droux, Jeanne, Reiss, Michael V, Weber, Bruno, Wegener, Susanne, Razansky, Daniel, University of Zurich, and Razansky, Daniel
- Subjects
1000 Multidisciplinary ,Multidisciplinary ,Microcirculation ,Skull ,10050 Institute of Pharmacology and Toxicology ,General Physics and Astronomy ,610 Medicine & health ,1600 General Chemistry ,Genetics and Molecular Biology ,General Chemistry ,General Biochemistry, Genetics and Molecular Biology ,3100 General Physics and Astronomy ,10040 Clinic for Neurology ,170 Ethics ,Mice ,Wide-field fluorescence microscopy ,Microscopy, Fluorescence ,1300 General Biochemistry, Genetics and Molecular Biology ,Biomedical engineering ,General Biochemistry ,Animals ,10237 Institute of Biomedical Engineering ,Tissue Distribution ,Ischemic Stroke - Abstract
Wide-field fluorescence imaging is an indispensable tool for studying large-scale biodynamics. Limited space-bandwidth product and strong light diffusion make conventional implementations incapable of high-resolution mapping of fluorescence biodistribution in three dimensions. We introduce a volumetric wide-field fluorescence microscopy based on optical astigmatism combined with fluorescence source localization, covering 5.6×5.6×0.6 mm3 imaging volume. Two alternative configurations are proposed exploiting multifocal illumination or sparse localization of point emitters, which are herein seamlessly integrated in one system. We demonstrate real-time volumetric mapping of the murine cortical microcirculation at capillary resolution without employing cranial windows, thus simultaneously delivering quantitative perfusion information across both brain hemispheres. Morphological and functional changes of cerebral vascular networks are further investigated after an acute ischemic stroke, enabling cortex-wide observation of concurrent collateral recruitment events occurring on a sub-second scale. The reported technique thus offers a wealth of unmatched possibilities for non- or minimally invasive imaging of biodynamics across scales., Nature Communications, 13 (1), ISSN:2041-1723
- Published
- 2022