1. Biochemical and blood gas alterations in buffalo (Bubalus bubalis) whole blood stored in CPDA-1 and CPD/SAG-M bags
- Author
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Osvaldo Gato Nunes Neto, Gilson Andrey Siqueira Pinto, Adriana Caroprezo Morini, Thamiris Souza de Alencar Silva, Antonio Humberto Hamad Minervino, Rejane dos Santos Sousa, Juliana Machado Portela, Enrico Lippi Ortolani, Francisco Leonardo Costa Oliveira, and Raimundo Alves Barrêto Júnior
- Subjects
Male ,Erythrocytes ,Buffaloes ,040301 veterinary sciences ,pCO2 ,Phosphates ,0403 veterinary science ,03 medical and health sciences ,0302 clinical medicine ,Animal science ,TRANSFUSÃO DE SANGUE ,Hemotherapy ,Medicine ,Animals ,Citrates ,Prospective Studies ,Total protein ,Whole blood ,General Veterinary ,biology ,Blood conservation ,business.industry ,Adenine ,Anticoagulants ,030208 emergency & critical care medicine ,04 agricultural and veterinary sciences ,Blood collection ,biology.organism_classification ,Glucose ,Blood Preservation ,Potassium ,Bubalus ,business ,Blood ph - Abstract
OBJECTIVE The objective of this study was to evaluate the biochemical and blood gas alterations of whole blood of buffaloes that was stored in citrate-phosphate-dextrose with adenine (CPDA-1) and CPD/SAG-M blood bags for 42 days. DESIGN Prospective study. INTERVENTIONS Ten male buffaloes were used in this study. A total volume of 900 mL of blood was collected from each buffalo so that 450 mL was stored in CPDA-1 and 450 mL was stored in CPD/SAG-M bags at 2-6°C for 42 days. The stored blood was evaluated at 7 time points (D): D0 (immediately after blood collection) and 7 (D7), 14 (D14), 21 (D21), 28 (D28), 35 (D35), and 42 (D42) days after collection. Blood gas, biochemical, and microbiological parameters were monitored. MEASUREMENTS AND MAIN RESULTS The overall blood pH decreased from 6.997 ± 0.05 at D0 to 6.784 ± 0.09 at D42, differing from baseline from D14 onward (P 0.05) evaluated, with the exception of pCO2 , HCO3, cholesterol, and total protein, which had higher values in the CPDA-1 bag (P
- Published
- 2019