1. Differentiation of Bone Marrow Mesenchymal Stem Cells into Neural Lineage Cells Induced by bFGF-Chitosan Controlled Release System
- Author
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Li, Manli, Zhao, Wen, Gao, Yudan, Hao, Peng, Shang, Junkui, Duan, Hongmei, Yang, Zhaoyang, and Li, Xiaoguang
- Subjects
MAPK/ERK pathway ,Article Subject ,lcsh:Medicine ,Bone Marrow Cells ,Fibroblast growth factor ,General Biochemistry, Genetics and Molecular Biology ,Immediate-Early Proteins ,Neural Stem Cells ,Animals ,Cell Lineage ,Inducer ,Receptor, Fibroblast Growth Factor, Type 1 ,Receptor ,Cell Proliferation ,Neurons ,Regulation of gene expression ,Chitosan ,Mitogen-Activated Protein Kinase 3 ,BTG2 ,General Immunology and Microbiology ,Cell growth ,Chemistry ,Tumor Suppressor Proteins ,Fibroblast growth factor receptor 1 ,lcsh:R ,Gene Expression Regulation, Developmental ,Cell Differentiation ,Mesenchymal Stem Cells ,General Medicine ,Rats ,Cell biology ,Fibroblast Growth Factor 2 ,Proto-Oncogene Proteins c-fos ,Research Article - Abstract
Bone marrow mesenchymal stem cells undergo differentiation to different lineages with different efficiencies when induced by different factors. We added a bFGF-chitosan controlled release system (bFGF-CCRS) as an inducer into conditioned medium to facilitate the oriented differentiation of BMSCs into neural lineage cells (eventually mature neurons); furthermore, we synchronized BMSCs to the G0/G1 phase via serum starvation to observe the effect of the inducer on the differentiation direction and efficiency. The nonsynchronized group, chitosan alone (not loaded with bFGF) group, soluble bFGF group, and conditioned medium group served as controls, and we observed the dynamic process of differentiation of BMSCs into neural lineage cells at different time points after the beginning of coculture. We analyzed the binding patterns of bFGF and chitosan and assayed the expression differences of key factors (FGFR1, ERK, and c-fos) and molecular switches (BTG2) that regulate the transformation from cell proliferation to differentiation. We also investigated the potential molecular mechanism of BMSC differentiation into neural lineage cells at a high percentage when induced by bFGF-CCRS.
- Published
- 2019
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