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This paper introduces the UI interface design and its application based on the iOS platform of comprehensively informationized teaching system. On the basis of typical examples of interfaces, results of user’s questionnaire and relevant theories, this paper attempts to present the UI interface in detail through the stages of discussion, design and implement. Meanwhile, in order to find balance between theoretical strictness and practical effectiveness, this paper will make every effort to point out the improvement methods and put forward solutions to the defect, according to the analysis of design under usability experience criterion.