1. Biocompatible surgical meshes based on decellularized human amniotic membrane
- Author
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Lei Hou, Gao Mengna, Yabin Zhu, Jun Wang, Peina Shi, and Qiuxia Shen
- Subjects
Materials science ,Biocompatibility ,Polyesters ,Myocytes, Smooth Muscle ,Polyurethanes ,Bioengineering ,Biocompatible Materials ,Polypropylenes ,Biomaterials ,Extracellular matrix ,chemistry.chemical_compound ,In vivo ,Materials Testing ,Animals ,Humans ,DAPI ,Amnion ,Lipase ,Decellularization ,Deoxyribonucleases ,biology ,Anatomy ,Prostheses and Implants ,Surgical Mesh ,In vitro ,Hypertonic saline ,Extracellular Matrix ,chemistry ,Mechanics of Materials ,biology.protein ,Rabbits ,Biomedical engineering - Abstract
Meshes play important roles to repair human tissue defect. In this work, human amniotic membrane (HAM) was decellularized and explored the efficacy as an implantable biological mesh. Surfactant, hypertonic saline, lipase and DNAase were used individually or collectively to remove all cell components and remain the extracellular matrix. Results of H&E and DAPI staining demonstrated that the method of surfactant and lipase combining with DNAase is the most effective treatment for HAM decellularization. Primary smooth muscle cells were seeded to evaluate the decellularized HAM's (dHAM) in vitro cytocompatibility. The in vivo test was performed via implantation at rabbits' uterus with clinic polypropylene mesh (PP) as the control. The results indicated that dHAM possessed good biocompatibility and will be a potential candidate for biological mesh.
- Published
- 2014