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Your search keyword '"GOMEZ-FLORES, RICARDO"' showing total 3 results
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3 results on '"GOMEZ-FLORES, RICARDO"'

1. In vitro antitumor, pro-inflammatory, and pro-coagulant activities of Megalopyge opercularis J.E. Smith hemolymph and spine venom

Author

Orozco-Flores, Alonso A., Valadez-Lira, José A., Covarrubias-Cárdenas, Karina E., Pérez-Trujillo, José J., Gomez-Flores, Ricardo, Caballero-Hernández, Diana, Tamez-Guerra, Reyes, Rodríguez-Padilla, Cristina, and Tamez-Guerra, Patricia

Subjects
0301 basic medicine, T-Lymphocytes, Immunology, Anti-Inflammatory Agents, lcsh:Medicine, Antineoplastic Agents, Venom, Biology, Peripheral blood mononuclear cell, Article, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Hemolymph, Animals, Humans, Cytotoxic T cell, lcsh:Science, Arthropod Venoms, Cell Proliferation, Multidisciplinary, Ecology, Coagulants, lcsh:R, Acridine orange, Molecular biology, Coagulation system, Haematopoiesis, 030104 developmental biology, chemistry, Cell culture, Apoptosis, 030220 oncology & carcinogenesis, Cytokines, Tumour immunology, DNA fragmentation, lcsh:Q, Immunotherapy, Chemokines
Abstract

Contact with stinging spines venom from several Lepidoptera larvae may result in skin lesions. In Mexico, envenomation outbreaks caused by Megalopyge opercularis were reported between 2015 and 2016. The aim of this study was to identify the venomous caterpillars in Nuevo Leon, Mexico and evaluate several biological activities of their hemolymph (HEV) and spine setae (SSV) venoms. M. opercularis was identified by cytochrome oxidase subunit (COI) designed primers. HEV and SSV extracts cytotoxic activity was assessed on the L5178Y-R lymphoma cell line. For apoptotic cells number and apoptosis, cells were stained with acridine orange/ethidium bromide and validated by DNA fragmentation. Human peripheral blood mononuclear cells (hPBMC) cytokine response to the extracts was measured by the cytometric bead array assay. Extracts effect on pro-coagulation activity on human plasma was also evaluated. HEV and SSV extracts significantly inhibited (p p

Published
2020

2. In vitro antimicrobial activity of Ruta chalepensis methanol extracts against the cariogenic Streptococcus mutans

Author

A Gloria Garza Marcela, A De La Garza Ramos Myriam, Rodriacute guez Padilla Cristina, Tamez Guerra Reyes, Tamez Guerra Patricia, Gomez Flores Ricardo, and Quintanilla Licea Ramiro

Subjects
Melioidosis, biology, Burkholderia pseudomallei, medicine.drug_class, Plant Science, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, biology.organism_classification, Antimicrobial, Monoclonal antibody, medicine.disease, Microbiology, Streptococcus mutans, Infectious Diseases, Ruta chalepensis, biology.protein, medicine, bacteria, Antibody, Flagellin
Abstract

Bulkholderia pseudomallei, the pathogeny of melioidosis, is naturally occurring in soil and water in epidemic regions. The environmental distribution of B. pseudomallei is still unclear, as indeed is the global distribution of B. pseudomallei. To detect B. pseudomallei in epidemic regionsi¼Œa double antibodies sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was developed to detect B. pseudomallei in soil cultures directly using two monoclonal antibodies (Mab) specific to recombinant truncated flagellin of B. pseudomallei. The method was evaluated by detecting the 154 soil samples collected from the Guangxi region in China where the endemic of melioidosis is. The results indicated that the sandwich ELISA method is not only faster than culture method which is the gold standard for B. pseudomallei detection in soil, but also a more sensitive method. It provides a new tool to detect B. pseudomallei in soil samples and is applicable for large screening studies to assess the distribution of B. pseudomallei in the endemic of melioidosis. Key words: Burkholderia pseudomallei, melioidosis, enzyme-linked immunosorbent assay (ELISA), soil sample.

Published
2013

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