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In the framework of the STIMTEC and STIMTEC-X hydraulic stimulation experiments at the Reiche Zeche mine, Freiberg (Germany), we installed acoustic emission (AE) sensors for the recording of picoseismicity both conventionally using pneumatic coupling and experimentally like a hydrophone, i.e. the sensors were placed in the borehole without a further coupling system or cementing. We investigate performance measures of the hydrophone-like acoustic emission (HAE) sensors such as frequency bandwidth, sensitivity, first motion polarity, coupling and placement quality to assess the sensor’s applicability in adaptive monitoring networks. HAE sensors can be paired with hydraulic equipment, especially with the double packer probe used for stimulation at the decametre scale because the monitored frequency content differs from injection-related noise. This offers a unique opportunity to improve the network geometry and consequently the quality of a seismic catalogue. We analyse the sensor characteristics using active ultrasonic transmission measurements from boreholes with different orientations in the rock volume, noise measurements preceding active centre punch hits in the access galleries and passive recordings of induced acoustic emission events. HAE sensors placed in water-filled boreholes show good sensitivity performance even without optimal coupling to the crystalline rock for recording distances up to 17 m. The HAE sensors record the wavefield adequately for first-arrival identification, polarity picking and amplitude characteristics but are less suitable for detecting S-waves. Due to the borehole geometry HAE sensors record waves with incidence angles from the side, resulting in opposite polarity compared to side-view AE sensors as observed in the field and lab. We discuss the advantages of adaptive monitoring networks with HAE sensors being optimally placed for each stimulation interval configuration anew to improve seismic event detection and quality of event hypocentre locations during hydraulic stimulations. We show that we are able to significantly reduce the azimuthal gap, halve the location uncertainties and improve the network coverage for the purpose of focal mechanism estimations.

Maar-diatreme volcanoes are small volcanic structures with a conical crater surrounded by a tephra-ring.They are formed by the explosive phreatomagmatic eruptions in a short timewhen groundwater comes into the contact with magma. We focus on theBažina maarin the geodynamically active western Eger Rift (Czech Republic) anddiscuss results from multidisciplinary geophysical investigations calibrated by drilling in the frame of the ICDP Eger project (International Continental Drilling Program). Apart from the internal structure, we show how detailed pre-drill geophysical research contributed to a proper selection of the drill site which concluded in revealing unique structure of a maar volcano with several generations of eruptions.Two boreholes located in the maar crater evinced lithologies of the maar-diatreme filling and its contact with country rocks. They penetrated volcanic rocks and volcaniclastic deposits of the maar but also revealed pyroclastic cone with the olivine nephelinite feeding conduit. Such results are unique and disclose that the maar formation was followed by several subsequent eruptions within the maar. Further investigationsconfirmed long-lasting volcanic activity with very primitive magma and extremely low magma productivity, whichdistinguishes the western Eger Rift fromother Cenozoic volcanic provinces in Europe.These results improve our understanding of volcanic processes, their significance is also general because they shed light on unique evolution of two volcanoes, one inside the other., The 28th IUGG General Assembly (IUGG2023) (Berlin 2023)

Poster: Speech Signal Enhancement in Cocktail Party Scenarios by Deep Learning based Virtual Sensing of Head Mounted Microphones

In order to gauge the role that active galactic nuclei play in the evolution of galaxies via the effect of kinetic feedback in nearby QSO 2’s (z ∼ 0.3), we observed eight such objects with bolometric luminosities Lbol∼1046ergs−1 using Gemini Multi-Object Spectrograph-integral field units. The emission lines were fitted with at least two Gaussian curves, the broadest of which we attributed to gas kinetically disturbed by an outflow. We found that the maximum extent of the outflow ranges from ∼1 to 8 kpc, being ∼0.5±0.3 times the extent of the [OIII] ionized gas region. Our ‘default’ assumptions for the gas density (obtained from the [SII] doublet) and outflow velocities resulted in peak mass outflow rates of M˙defout∼ 3–30 M⊙yr−1 and outflow power of E˙defout∼1041–1043ergs−1⁠. The corresponding kinetic coupling efficiencies are εdeff=E˙defout/Lbol∼7×10−4–0.5 per cent, with the average efficiency being only 0.06 per cent (0.01 per cent median), implying little feedback powers from ionized gas outflows in the host galaxies. We investigated the effects of varying assumptions and calculations on M˙out and E˙out regarding the ionized gas densities, velocities, masses, and inclinations of the outflow relative to the plane of the sky, resulting in average uncertainties of 1 dex. In particular, we found that better indicators of the [OIII] emitting gas density than the default [SII] line ratio, such as the [ArIV] λλ4711,40 line ratio, result in almost an order of magnitude decrease in the εf. Peerreview

Lenalidomide, an antineoplastic and immunomodulatory drug, has therapeutic activity in acute myeloid leukemia (AML), but definitive studies about its therapeutic utility have been lacking. In a phase 3 study, we compared 2 induction regimens in newly diagnosed patients age 18 to 65 years with AML: idarubicine-cytarabine (cycle 1) and daunorubicin and intermediate-dose cytarabine (cycle 2) without or with lenalidomide (15 mg orally on days 1-21). One final consolidation cycle of chemotherapy or autologous stem cell transplantation (auto-SCT) or allogeneic SCT (allo-SCT) was provided according to a prognostic risk and minimal residual disease (MRD)-adapted approach. Event-free survival (EFS; primary end point) and other clinical end points were assessed. A second random assignment in patients in complete response or in complete response with incomplete hematologic recovery after cycle 3 or auto-SCT involved 6 cycles of maintenance with lenalidomide (10 mg on days 1-21) or observation. In all, 392 patients were randomly assigned to the control group, and 388 patients were randomly assigned to lenalidomide induction. At a median follow-up of 41 months, the study revealed no differences in outcome between the treatments (EFS, 44% ± 2% standard error and overall survival, 54% ± 2% at 4 years for both arms) although in an exploratory post hoc analysis, a lenalidomide benefit was suggested in SRSF2-mutant AML. In relation to the previous Dutch-Belgian Hemato-Oncology Cooperative Group and Swiss Group for Clinical Cancer Research (HOVON-SAKK) studies that used a similar 3-cycle regimen but did not pursue an MRD-guided approach, these survival estimates compare markedly more favorably. MRD status after cycle 2 lost prognostic value in intermediate-risk AML in the risk-adjusted treatment context. Maintenance with lenalidomide showed no apparent effect on relapse probability in 88 patients randomly assigned for this part of the study. ispartof: BLOOD ADVANCES vol:5 issue:4 pages:1110-1121 ispartof: location:United States status: published

The U.S. Naval Observatory (USNO), in collaboration with Paris Observatory (OP), is conducting the Fundamental Reference AGN Monitoring Experiment, or FRAMEx. FRAMEx will use USNO's and OP's in-house observing assets in the radio, infrared (IR) and visible, as well as other ground- and space-based telescopes (e.g., in the X-ray) that we can access for these purposes, to observe and monitor current and candidate Reference Frame Objects (RFOs) -- consisting of Active Galactic Nuclei (AGN) -- as well as representative AGN, in order to better understand astrometric and photometric variability at multiple timescales. FRAMEx will improve the selection of RFOs as well as provide significant new data to the AGN research community. This paper describes the FRAMEx objectives, specific areas of investigation, and the initial data collection campaigns., Comment: 8 pages, 2 figures. To be published in the Proceedings of 2019 Journ\'ees meeting

An upsurge in Echovirus 30 (E30) infections, associated with meningitis/meningoencephalitis, has been observed in Denmark, Germany, the Netherlands, Norway and Sweden in the period April to September 2018, compared with 2015-2017. In total, 658 E30 infections among 4,537 enterovirus infections were detected in 15 countries between January and September 2018 and affected mainly newborns and 26-45 year-olds. National public health institutes are reminded to remain vigilant and inform clinicians of the ongoing epidemic. ispartof: EUROSURVEILLANCE vol:23 issue:44 pages:2-13 ispartof: location:Sweden status: published

To understand people and their intentions, humans have developed the ability to imagine their surroundings from another visual point of view. This cognitive ability is called perspective taking and has been shown to be essential in child development and social interactions. However, the precise cognitive mechanisms underlying perspective taking remain to be fully understood. Here we present a computa- tional model that implements perspective taking as a mental simulation of the physical movements required to step into the other point of view. The visual percept after each mental simulation step is estimated using a set of forward models. Based on our experimental results, we propose that a visual attention mechanism explains the response times reported in human visual perspective taking experiments. The model is also able to generate several testable predictions to be explored in further neurophysiological studies.

© 2017 Royal College of Obstetricians and Gynaecologists Objective: Develop a core outcome set of international consensus definitions for severe maternal morbidities. Design: Electronic Delphi study. Setting: International. Population: Eight expert panels. Methods: All 13 high-income countries represented in the International Network of Obstetric Surveillance Systems (INOSS) nominated five experts per condition of morbidity, who submitted possible definitions. From these suggestions, a steering committee distilled critical components: eclampsia: 23, amniotic fluid embolism: 15, pregnancy-related hysterectomy: 11, severe primary postpartum haemorrhage: 19, uterine rupture: 20, abnormally invasive placentation: 12, spontaneous haemoperitoneum in pregnancy: 16, and cardiac arrest in pregnancy: 10. These components were assessed by the expert panel using a 5-point Likert scale, following which a framework for an encompassing definition was constructed. Possible definitions were evaluated in rounds until a rate of agreement of more than 70% was reached. Expert commentaries were used in each round to improve definitions. Main outcome measures: Definitions with a rate of agreement of more than 70%. Results: The invitation to participate in one or more of eight Delphi processes was accepted by 103 experts from 13 high-income countries. Consensus definitions were developed for all of the conditions. Conclusion: Consensus definitions for eight morbidity conditions were successfully developed using the Delphi process. These should be used in national registrations and international studies, and should be taken up by the Core Outcomes in Women's and Newborn Health initiative. Tweetable abstract: Consensus definitions for eight morbidity conditions were successfully developed using the Delphi process.

Gammaproteobacterial Reinekea spp. were detected during North Sea spring algae blooms in the years 2009-2012, with relative abundances of up to 16% in the bacterioplankton. Here, we explore the ecophysiology of ‘R. forsetii' strain Hel1_31_D35 that was isolated during the 2010 spring bloom using (i) its manually annotated, high-quality closed genome, (ii) re-analysis of in situ data from the 2009–2012 blooms and (iii) physiological tests. High resolution analysis of 16S rRNA gene sequences suggested that ‘R. forsetii' dominated Reinekea populations during these blooms. This was corroborated by retrieval of almost complete Hel1_31_D35 genomes from 2009 and 2010 bacterioplankton metagenomes. Strain Hel1_31_D35 can use numerous low-molecular weight substrates including diverse sugar monomers, and few but relevant algal polysaccharides such as mannan, α-glucans, and likely bacterial peptidoglycan. It oxidizes thiosulfate to sulfate, and ferments under anoxic conditions. The strain can attach to algae and thrives at low phosphate concentrations as they occur during blooms. Its genome encodes RTX toxin and secretion proteins, and in cultivation experiments Hel1_31_D35 crude cell extracts inhibited growth of a North Sea Polaribacter strain. Our data suggest that the combination of these traits make strain Hel1_31_D35 a versatile opportunist that is particularly competitive during spring phytoplankton blooms.

Various research topics are emerging as the demand for intelligent lifelong interactions between robot and humans increases. Among them, we can find the examination of persistent storage, the continuous unsupervised annotation of memories and the usage of data at high-frequency over long periods of time. We recently proposed a lifelong autobiographical memory architecture tackling some of these challenges, allowing the iCub humanoid robot to 1) create new memories for both actions that are self-executed and observed from humans, 2) continuously annotate these actions in an unsupervised manner, and 3) use reasoning modules to augment these memories a-posteriori. In this paper, we present a reasoning algorithm which generalises the robots’ understanding of actions by finding the point of commonalities with the former ones. In particular, we generated and labelled templates of pointing actions in different directions. This represents a first step towards the emergence of a procedural memory within a long-term autobiographical memory framework for robots.

In the future, robots will support humans in their every day activities. One particular challenge that robots will face is understanding and reasoning about the actions of other agents in order to cooperate effectively with humans. We propose to tackle this using a developmental framework, where the robot incrementally acquires knowledge, and in particular 1) self-learns a mapping between motor commands and sensory consequences, 2) rapidly acquires primitives and complex actions by verbal descriptions and instructions from a human partner, 3) discovers correspondences between the robots body and other articulated objects and agents, and 4) employs these correspondences to transfer the knowledge acquired from the robots point of view to the viewpoint of the other agent. We show that our approach requires very little a-priori knowledge to achieve imitation learning, to find correspondent body parts of humans, and allows taking the perspective of another agent. This represents a step towards the emergence of a mirror neuron like system based on self-learned representations.

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Nuclear pasta, with nucleons arranged into tubes, sheets, or other complex shapes, is expected in core collapse supernovae (SNe) at just below nuclear density. We calculate the additional opacity from neutrino-pasta coherent scattering using molecular dynamics simulations. We approximately include this opacity in simulations of SNe. We find that pasta slows neutrino diffusion and greatly increases the neutrino signal at late times of 10 or more seconds after stellar core collapse. This signal, for a galactic SN, should be clearly visible in large detectors such as Super-Kamiokande., Comment: 6 pages, 4 figures

Using the 18.8 pb−1 of data accumulated at LEP in 1990 and 1991 with the ALEPH detector, a direct test of neutral current CP-invariance is performed by a search for CP-odd correlations in Z decays to τ pairs where both τ decay modes are identified. No evidence for CP-violation is observed. The weak dipole moment of the τ has been measured to be dτ(mZ) = (1.3 ± 1.4 ± 0.1) × 10−17 e·cm which results in an upper limit on the weak dipole moment of |dτ(mZ)| ⩽ 3.7 × 10−17 e·cm with 95% confidence level.

The aim of the study is to investigate which of two treatment options of saline lavage induced ARDS in rabbits is better in terms of oxygenation and prevention of barotrauma: combined high peak pressure ventilation with surfactant administration and inhaled nitric oxide or veno-venous ECMO combined with low peak inspiratory pressure ventilation.Materials and MethodsAfter saline lavage (10 cc/kg repeated as long as foamy retrieval was observed) two combined therapeutic strategies were examined: ventilation with high inspiratory pressures (35 cm H2O) with additional exogenous surfactant administration (100 mg/kg) and inhaled nitric oxide (10 PPM) (n=5, group 1) and low inspiratory pressure (20 cm H2O) ventilation under veno-venous ECMO support (n=5, group 2). The FiO2was maintained at 1.0 in both groups. The paO2/FiO2ratio was calculated in 30 minute intervals for 4 hours. After that the animals were sacrificed and the lungs examined macro- and microscopically. Aeration was described in a semiquantitative method using the alveolar expansion index. Oxygenation in group 1 was significantly better than in group 2, it increased significantly after surfactant but not after additional nitric oxide administration. However, the lungs in group 1 showed severe signs of baro/ergotrauma (Hyaline membranes, air leaks, infiltration of polymorphonuclear (PMN) granulocytes and macrophages, break down of alveolar capillary membranes) after 4 hrs of combined therapy, whereas the lungs in group 2 appeared normal. Adding surfactant and NO to a high tidal volume ventilation improved oxygenation, but did not prevent baro/ergotrauma. Ventilation with low inspiratory pressures combined with ECMO caused little baro/ergotrauma but adequate oxygenation could not be achieved, probably due to anatomical features of the rabbit which do not allow appropriate blood flow within the ECMO-circuit.

Aim : To compare the efficacy of simethicone with cisapride in patients with functional (non-ulcer) dyspepsia. Methods : After standardized diagnostic work-up and at least 6-days wash-out of medication, 177 patients with functional dyspepsia were enrolled; 173 of them (age 19–71 years) were randomized and treated using a double-dummy technique with simethicone (84 mg t.d.s.) or cisapride (10 mg t.d.s.). At baseline and after 2 and 4 weeks, the intensity of the symptoms was scored from 0 (absent) to 3 (severe) using a standardized symptom questionnaire. Efficacy of the treatment was judged by the patients as ‘very good’, ‘good’, ‘moderate’ or ‘no effect’. Results : A total of 166 patients completed the trial. After 2 and 4 weeks, 34% and 46% (respectively), of the patients treated with simethicone judged the improvement in symptoms to be excellent compared to 13% and 22% (respectively) of patients treated with cisapride (P

We report on an investigation of optical properties of multiferroic CoCr$_{2}$O$_{4}$ at terahertz frequencies in magnetic fields up to 30 T. Below the ferrimagnetic transition (94 K), the terahertz response of CoCr$_{2}$O$_{4}$ is dominated by a magnon mode, which shows a steep magnetic-field dependence. We ascribe this mode to an exchange resonance between two magnetic sublattices with different $g$-factors. In the framework of a simple two-sublattice model (the sublattices are formed by Co$^{2+}$ and Cr$^{3+}$ ions), we find the inter-sublattice coupling constant, $\lambda = - (18 \pm 1)$ K, and trace the magnetization for each sublattice as a function of field. We show that the Curie temperature of the Cr$^{3+}$ sublattice, $\Theta_{2}$ = $(49 \pm 2)$ K, coincides with the temperature range, where anomalies of the dielectric and magnetic properties of CoCr$_{2}$O$_{4}$ have been reported in literature., Comment: accepted to PRB

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Current knowledge about the quality and appropriateness of pharmacological pain treatment in nursing home residents (NHR), particularly in NHR with moderate to severe cognitive impairment, is poor. This observational cross-sectional study assessed pain treatment in a random sample of NHR with or without cognitive impairment from nursing homes in Germany. Prescribed drugs, pain intensity and frequency, diagnoses, and surgical procedures and injuries during the last 4 weeks were documented. Quality and appropriateness of pain medication were assessed by analysis of pain medications and the Pain Medication Appropriateness Scale (PMAS) score (S(PMAS) ), with a cut-off value of >67% indicating appropriate pain treatment. A total of 321 residents (62% women) were studied, including 152 (47%) with severe cognitive impairment. The most frequently prescribed analgesics were dipyrone, fentanyl, tramadol and ibuprofen. The mean S(PMAS) was 48.5 ± 1.5 (range, -33 to +100). Residents with prescribed scheduled analgesics had a significantly better S(PMAS) than patients without such treatment (S(PMAS) 58 ± 1.5 vs. 37 ± 2.5, p < 0.01). NHR without current pain had significantly better S(PMAS) than residents suffering from pain (S(PMAS) 47 ± 1.9 vs. 59 ± 4.2, p = 0.01). With an S(PMAS) of 69 ± 1.5, residents (n = 106) with scheduled pain medication plus PRN analgesics achieved the highest scores in the population. Overall, similar results were found in NHR with and in NHR without cognitive impairment. Our study points to a significant deficit in pain treatment in German NHR, including NHR with or without cognitive impairment. © 2011 European Federation of International Association for the Study of Pain Chapters.

We have measured the magnetization and specific heat of multiferroic CoCr2O4 in magnetic fields up to 14 T. The high-field magnetization measurements indicate a new phase transition at T* = 5 - 6 K. The phase between T* and the lock-in transition at 15 K is characterized by magnetic irreversibility. At higher magnetic fields, the irreversibility increases. Specific-heat measurements confirm the transition at T*, and also show irreversible behavior. We construct a field-temperature phase diagram of CoCr2O4., Comment: 4 pages

SOLAR II ist das zweite Follow-up einer bevölkerungsbezogenenden Kohorten-Studie. Diese Studie basiert auf einer Kohorte, die 1995/1996 aus damals 9-11jährigen Kindern aus Dresden und München zusammengestellt wurde. Die inzwischen erwachsenen Teilnehmer wurden nun erneut mit dem Ziel untersucht, Zusammenhänge zwischen beruflichen Expositionen und Allergien und Atemwegserkrankungen zu ermitteln. Ein Schwerpunkt der Auswertung galt der Frage, wie sich aus Risikofaktoren, die bereits in der Kindheit erkennbar sind, vorhersagen lässt, dass sich bei Tätigkeitsbeginn in Berufen mit hoher Exposition eine Allergie oder eine Atemwegserkrankung entwickeln wird. Mit den Ergebnissen der Studie kann nicht begründet werden, Jugendlichen mit Risikofaktoren für allergische Atemwegserkrankungen grundsätzlich von Tätigkeiten mit hohem Expositions-Potenzial abzuraten. Die Autoren sprechen sich aber für eine engmaschige arbeitsmedizinische Betreuung junger Erwachsener aus, die am Beginn einer solchen Tätigkeit stehen.

Relatively few studies have examined the relation of different hymenoptera sting reactions.To investigate the relation of anaphylactic reactions against stings of different hymenoptera subspecies in the Mediterranean population of Albania.A retrospective study was conducted using the clinic files of 111 patients who were diagnosed for hymenoptera sting reactions from 1987 to 1996. Antigens used consisted of purified hymenoptera venom (bee, wasp, and paperwasp). The patients were diagnosed by intracutaneous tests in concentrations of 0.001 microgram/ml, 0.01 microgram/ml, 0.1 microgram/ml, and 1 microgram/ml.The median age of the patients was 27 years. 57% of stings occurred between 20 to 40 years of age. The majority of anaphylactic reactions were recorded during the months of June to October, 81% of the patients were admitted to the hospital due to Mueller grade II to III reactions. In 26% of all cases, crossreactions (bee-wasp 16%, bee-wasp-paperwasp 7%, wasp-paperwasp 2%, bee-paperwasp 1%) were found. Of all anaphylactic reactions, 64% were attributed to bees, 24% to wasps, 8% to both bees and wasps, and 2% to paperwasps.In contrast to industrialized countries such as the United States or Western Europe where urban populations predominate, reactions to bee venom were more prevalent in the present study population.

Using the 18.8 pb-1 of data accumulated at LEP in 1990 and 1991 with the ALEPH detector, a direct test of neutral current CP-invariance is performed by a search for CP-odd correlations in Z decays to tau pairs where both tau decay modes are identified. No evidence for CP-violation is observed. The weak dipole moment of the tau has been measured to be d(tau)(m(Z)) = (1.3 +/- 1.4 +/- 0.1) x 10(-17)e.cm which results in an upper timit on the weak dipole moment of \d(tau)(m(Z))\ less-than-or-equal-to 3.7 x 10(-17)e.cm with 95% confidence level.

This paper summarises the development of a machine-readable model series for explaining Gaudi's use of ruled surface geometry in the Sagrada Familia in Barcelona, Spain. The first part discusses the modeling methods underlying the columns of the cathedral and the techniques required to translate them into built structures. The second part discusses the design and development of a tangible machine-readable model to explain column-modeling methods interactively in educational contexts such as art exhibitions. It is designed to explain the principles underlying the column design by means of physical interaction without using mathematical terms or language.

Resonance thromboelastography (RTG), a further development of the thromboelastogram (TEG), has been designed for improved differentiation of the effect of the plasmatic coagulation factors (increasing F-leg) and platelets (decreasing P-leg) on clot formation. It is based on the effect of clot elasticity on the resonance of a swinging wire. We assessed the RTG for its ability to differentiate coagulation disorders that frequently occur after cardiac surgery. The RTG was performed with a CS-3 Analyzer. Samples from 10 healthy volunteers were investigated after the following preparations: (1) baseline values, (2) dilution to a hematocrit of 30% and 20% with either hydroxyl ethyl starch (HES) 10% or plasma; (3) addition of 0.25, 0.5, and 1.0 IU/mL porcine heparin with and without heparinase; and (4) addition of 1.0, 3.0, 4.0, and 5.0 microg/mL of the antiplatelet agent abciximab (ReoPro). Increasing concentrations of abciximab led to a slower decrease or in the case of higher concentrations, to a persistent elevation of the platelet leg of the RTG. Dilution of the hematocrit with plasma had no effect on the fibrin and platelet leg; whereas, dilution with HES 10% led to an inhibition of the fibrin and platelet leg. Dilution of the plasmatic coagulation factors resulted in an inhibition of both the fibrin and the platelet leg. The addition of 0.25 and 0.5 IU/mL of heparin led to an increased coagulation time and inhibition of the fibrin and platelet legs. These effects were eliminated by the addition of heparinase. The RTG enables the evaluation of platelet function under the condition of a nonimpaired plasma coagulation system. Depletion of plasma coagulation factors and the administration of small amounts of heparin do not enable the distinction between residual effects of an anticoagulant, coagulation factor deficiency, or impaired platelet function. However, the heparin effects can be eliminated by the addition of heparinase. Further improvement may be achieved using a modified RTG by adding plasma coagulation factors in one channel for an improved evaluation of platelet function, even under the condition of a loss of procoagulants.

For in vivo determination of innate and memory immune reactions we have implanted sterile gelatin sponges subcutaneously in swine for the monitoring of the following immunological parameters: 1. Analysis of local cell population phenotypes after vascularization of the gelatin sponges using flow cytometry. 2. Comparative analysis of sponge-infiltrating cells after loading with viral antigen in primed as well as naive animals. 3. Performance of reverse transcription quantitative competitive PCR (RT-qcPCR) for the detection of porcine cytokine mRNA indicative for IFN-gamma, IL-2, IL-4, IL-8 and IL-10. The in vitro analysis, e.g. by re-exposure to viral antigens, allows the determination of cytokine reaction patterns of sponge derived cells, draining lymph node cells as well as PBMC of the same individual. Studies of innate reactions and modulation of cellular infiltration in transplanted gelatin sponges are possible. Functional assays, e.g. cell-mediated cytotoxicity, antigen specific cell proliferation, using sponge-derived cells will provide additional information about the suitability of the model for example in vaccine potency tests.