Raman spectroscopy is a very powerful tool for material analysis, allowing for exploring the properties of a wide range of different materials. Since its discovery, Raman spectroscopy has been used to investigate several features of materials such carbonaceous and inorganic properties, providing useful information on their phases, functions, and defects. Furthermore, techniques such as surface and tip enhanced Raman spectroscopy have extended the field of application of Raman analysis to biological and analytical fields. Additionally, the robustness and versatility of Raman instrumentations represent a promising solution for performing on-field analysis for a wide range of materials. Recognizing the many hot applications of Raman spectroscopy, we herein overview the main and more recent applications for the investigation of a wide range of materials, such as carbonaceous and biological materials. We also provide a brief but exhaustive theoretical background of Raman spectroscopy, also providing deep insight into the analytical achievements.