Search

Alginate lyases play an important role in alginate oligosaccharides (AOS) preparation and brown seaweed processing. Many extracellular alginate lyases have been characterized to develop efficient degradation tools needed for industrial applications. However, few studies focusing on intracellular alginate lyases have been conducted. In this work, a novel intracellular alkaline alginate lyase Alyw202 from Vibrio sp. W2 was cloned, expressed and characterized. Secretory expression was performed in a food-grade host, Yarrowia lipolytica. Recombinant Alyw202 with a molecular weight of approximately 38.3 kDa exhibited the highest activity at 45 &deg, C and more than 60% of the activity in a broad pH range of 3.0 to 10.0. Furthermore, Alyw202 showed remarkable metal ion-tolerance, NaCl independence and the capacity of degrading alginate into oligosaccharides of DP2-DP4. Due to the unique pH-stable and high salt-tolerant properties, Alyw202 has potential applications in the food and pharmaceutical industries.

In electrolytes TiO2-based complex ceramic coatings containing Ca and P on titanium alloy were ultimately formed containing zirconia/hydroxyapatite by Micro-arc oxidation process. As well as the biomechanics measure experiment of speciments had been done, and the datas were analysised and researched. In addition, the toughening mechanism of reinforced coating was studied in detail, and consists of the second phase toughening, phase transformation toughening and microcrack toughening.The results show that the zirconia /hydroxyapatite coating has advanced biomechanics propertys compared to the individual hydroxyapatite one.

A medicinal titanium alloy hydroxyapatite coating was fabricated by microarc oxidation process, and a kind standard of simulated body fluid was configured, after that the biocompatibility experiment was carried out. The soaked coatings surface morphologies were observed and analysed by scanning electron microscopy,and the coating spectra were simultaneityly researched by X-ray diffraction. The instance of degradation and precipitate were investigated,and the transformation of Ca/P value were measured.