1. CRISPR-Cas Systems Impact Pseudomonas aeruginosa Genome Structure
- Author
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LaFave, Matthew C., Belkum, Alex Van, Soriaga, Leah B., Srividya Akella, E. Magda Barbu, Hannum, Gregory, Miller, Kristofer, Enright, Mark C., Brami, Daniel, Felderman, Martina, Schwartz, Ariel, Richardson, Toby H., Peterson, Todd C., Bolyn Hubby, and Cady, Kyle C.
- Abstract
Pseudomonas aeruginosa is both an antibiotic-resistant opportunistic pathogen and an important model of type I clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated protein (CRISPR-Cas) systems. Comparative genomics has identified several CRISPR-Cas subtypes, and it was previously unclear how these immune modules might influence the genome content of P. aeruginosa. To better understand the distribution of CRISPR-Cas subtypes and their impact on genome composition, we annotated 672 P. aeruginosa clinical isolates. We found that CRISPR-Cas systems modulate genome size and accessory elements. In addition, we identified a novel, putatively mobile type I-C CRISPR-Cas system. In the process, we also created a global spacer library that provides a new means of identifying accessory fragments, and facilitates CRISPR typing of many P. aeruginosa strains. Finally, we have made the assemblies of 282 newly-sequenced P. aeruginosa isolates public as an NCBI BioProject (ID: PRJNA297679).
- Published
- 2016
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