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The overall objective of this study was to determine the dose response to four levels of cashew nut shell extract in a granulated form (CNSE, containing 59% anacardic acid and 18% cardol) on culture pH, rumen fermentation metabolites, and apparent nutrient digestibility in continuous culture fermenters. The study was conducted as a generalized randomized complete block design with four treatments and four replications per treatment. The four treatments were randomly assigned to eight fermenters for two incubation runs of 10 d. Treatments consisted of (1) Control (CO, no CNSE), (2) Control plus 100 ppm of CNSE, (3) Control plus 200 ppm of CNSE, and (4) Control plus 300 ppm of CNSE. Fermenters were fed 52 g/d (DM basis) of a total mixed ration (TMR; 17.0% crude protein (CP), 29.7% NDF, and 29.9% starch), divided between two feedings at 0800 and 2000 h. The apparent digestibility of dry matter (DM), organic matter (OM), and neutral detergent fiber (NDF) were not affected by CNSE supplementation. Similarly, CNSE had no effect on culture pH, total volatile fatty acids (VFA) or individual VFA molar proportions. These results suggest that at the dosages evaluated in this study, CNSE has no impact on the rumen fermentation profile and the apparent nutrient digestibility under continuous culture conditions.

In a feeding experiment for 75 days, the effects of fumitory (Fumaria officinalis) extract on growth, haematology, immune response and antioxidant enzyme activities in rainbow trout (Oncorhynchus mykiss) were evaluated. The aqueous methanolic extract of the plant was mixed with feeds at three different levels, 0.1% (FO1), 0.2% (FO2) and 0.3% (FO3), and feed with no plant extract donated as the control. All four groups (mean initial weight: 13.02 ± 0.02 g) were fed with the prepared diets twice daily ad libitum in a triplicate experiment. Blood and liver samples were taken from the fish on 15, 30, 45, 60 and 75 days. Also, overall growth parameters were determined based on body weight data recorded from all fish before and at the end of feeding trial. Results indicated that there were variations in nonspecific immune parameters (lysozyme, myeloperoxidase and oxidative radical production), but supplementation of the plant extract did not affect the immune response of the fish significantly. On the other hand, there was a general increase in the antioxidant enzyme activities (superoxide dismutase, catalase, glutathione peroxidase, and glucose-6-phosphate dehydrogenase) evaluated in this study. Furthermore, possibly the most remarkable finding of the study is that the growth performance in the FO3 group was roughly 30% higher than that of the control group. Considering all findings in the present study, we conclude that 0.3% supplementation of fumitory extract would be beneficial for rainbow trout farming with respect to elevated growth and antioxidant status.

Salmonella is the leading cause of bacterial foodborne zoonoses in humans. Thus, the development of strategies to control bacterial pathogens in poultry is essential. Peanut skins, a considerable waste by-product of the peanut industry is discarded and of little economic value. However, peanut skins contain identified polyphenolic compounds that have antimicrobial properties. Hence, we aim to investigate the use of peanut skins as an antibacterial feed additive in the diets of broilers to prevent the proliferation of Salmonella Enteritidis (SE). One hundred sixty male hatchlings (Ross 308) were randomly assigned to (i) peanut skin diet without SE inoculation (PS); (ii) peanut skin diet and SE inoculation (PSSE); (iii) control diet without SE inoculation (CON); and (iv) control diet with SE inoculation (CONSE). Feed intake and body weights were determined at weeks 0 and 5. On days 10 and 24 posthatch, three birds per pen (24 total) from each treatment group were euthanized, and the liver, spleen, small intestine, and ceca were collected. The weights of the liver, spleen, and ceca were recorded. Organ invasion was determined by counting SE colonies. Each pen served as an experimental unit and was analyzed by using a t test. Performance data were analyzed in a completely randomized design by using a general linear mixed model to evaluate differences. There were no significant differences (P0.05) in weekly average pen body weight, total feed consumption, bird weight gain, and feed conversion ratio between the treatment groups. There were no significant differences in SE CFU per gram for fecal, litter, or feed between the treatment groups CONSE and PSSE. However, for both fecal and litter, the PSSE treatment group tended (P ≤ 0.1) to have a lower Salmonella CFU per gram compared with the CONSE treatment group. The results indicate that peanut skins may have potential application as an antimicrobial feed additive to reduce the transmission or proliferation of SE in poultry environments or flocks.

Aim This study was conducted to test the ability of a carvacrol-based formulation (Phodé, France) to decrease the C. jejuni caecal load in inoculated broiler chickens and to study the impact of the C. jejuni inoculation alone or combined with the product, on the caecal microbiota. Methods and Results On day 1, chickens were either fed a control feed or the same diet supplemented with a carvacrol-based product. On day 21, the carvacrol-supplemented chickens and half of the non-supplemented chickens were inoculated with C. jejuni (108 CFU). Quantitative PCR was used to quantify C. jejuni in chicken caecal samples and 16S rRNA gene sequencing was carried out at 25, 31 and 35 days of age. A significant decrease of 1.4 log of the C. jejuni caecal load was observed in 35-day-old chickens supplemented with the product, compared to the inoculated and unsupplemented group (p < 0.05). The inoculation with C. jejuni significantly increased the population richness, Shannon and Simpson diversity and altered beta-diversity. Compared to the control group, the C. jejuni inoculation causes significant changes in the microbiota. The carvacrol-based product associated with C. jejuni inoculation increased the diversity and strongly modified the structure of the microbial community. Functional analysis by 16S rRNA gene-based predictions further revealed that the product up-regulated the pathways involved in the antimicrobial synthesis, which could explain its shaping effect on the caecal microbiota. Conclusions Our study confirmed the impairment of the caecal bacterial community after inoculation and demonstrated the ability of the product to reduce the C. jejuni load in chickens. Further investigations are needed to better understand the mode of action of this product to promote the installation of a beneficial microbiota to its host. Significance and Impact of the Study Results suggested that this product could be promising to control C. jejuni contamination of broilers.

The objective of this study was to determine the effectiveness of mycotoxin management with feed additive by monitoring biochemical blood parameters, reproduction status and udder health in cows. During the first 1-12-months, the reproduction performance was assessed. The cows were fed only total mixed ration (TMR) with naturally contaminated mycotoxins (ZEN;DON;AFB1;OTA) and the cows were regarded as a control group (CG). In months 13-15, two groups were created: control (CG)(n=30) and experimental (EG)(n=60). The CG was fed with contaminated TMR and the EG was fed with the same TMR+40g/cow mycotoxins management feed additive (TMXL1000). During this period, blood indicators and udder health were studied. Beginning with months 16-24, all cows were fed with contaminated TMR+40 g/cow (TMXL1000) and regarded as the EG. The IgA concentrations in the CG decreased in the 15th month (p

A. bilimbi fruit filtrate, wheat bran, and S. cerevisiae contain bioactive components favorable to broiler health. The use of these compounds in combination was expected to exert synergistic effects on broilers. The study investigated the effect of a combination of A. bilimbi fruit filtrate, wheat bran, and S. cerevisiae on haematological indices and intestinal selected bacteria and morphology of broilers. A total of 280 broiler chicks were randomly divided into 4 groups with 7 replications, including CONT (chicks offered diet without additive), TBLEND1, BLEND2, and BLEND3 (chicks offered diet with 0.25%, 0.5%, and 1% of the additive combination of A. bilimbi fruit filtrate, wheat bran, and S. cerevisiae), respectively. For data collection, the chicks were blood sampled at day 21 and 35, and slaughtered at day 35. The data were statistically treated with analysis of variance according to a completely randomized design. On day 21, the erythrocytes and haemoglobin levels were lower in BLEND2 and BLEND3 than those in CONT and BLEND1 (p

Background and Aim: Antibiotic, improves the growth performance of laying hens when used as a feed additive; however, it has been banned in Europe. Furthermore, secondary metabolites used as a substitute for antibiotics are compounds produced by plants. Therefore, this aims to determine the effect of dates water extract (DWE) on the performance of laying hens. This study used dates containing secondary metabolites as a feed additive and substitute for antibiotics. Materials and Methods: A completely randomized design was used, dividing 400 Lohmann brown day old chick into five groups (each group has five replications and each replication consisted of 16 laying hens). Furthermore, there were two control groups such as mineral water control group and antibiotic growth promoters (basal feed+50 mg/kg of bacitracin), and three DWE groups such as 5% DWE (50 mg/mL), 10% DWE (100 mg/mL), and 20% DWE (200 mg/mL). Dates extract treatment was administered through drinking water for 54 days, whereby three laying hens from each replication were taken randomly and decapitated on the neck. Afterwards, a necropsy was performed for histological preparations of the small intestine, ovary, and lymphoid organs. The structure and morphology of the small intestine, and ovaries were observed through histological preparations, while lymphoid organs were observed through histological preparation and morphometry, and body morphometry, body weight, feed intake and weight gain were observed by measurements and weighing. Results: Small intestine morphology, ovarian follicle, and growth performance of the DWE2 group increased significantly compared to the control group, but the lymphoid organs index was influenced by DWE1. Conclusion: The administration of 10% dates extract (100 mg/mL) in drinking water improves the morphology of the small intestine, ovarian follicles, lymphoid organs, and growth performance.

Antibiotic growth promoters have been utilized for long time at subtherapeutic levels as feed supplements in monogastric animal rations. Because of their side-effects such as antibiotic resistance, reduction of beneficial bacteria in the gut, and dysbiosis, it is necessary to look for non-therapeutic alternatives. Probiotics play an important role as the key substitutes to antibacterial agents due to their many beneficial effects on the monogastric animal host. For instance, enhancement of the gut microbiota balance can contribute to improvement of feed utilization efficiency, nutrients absorption, growth rate, and economic profitability of livestock. Probiotics are defined as “live microorganisms that, when administered in adequate amounts, confer a health benefit on the host.” They are available in diverse forms for use as feed supplements. Their utilization as feed additives assists in good digestion of feed ingredients and hence, making the nutrients available for promoting growth. Immunity can also be enhanced by supplementing probiotics to monogastrics diets. Moreover, probiotics can help in improving major meat quality traits and countering a variety of monogastric animals infectious diseases. A proper selection of the probiotic strains is required in order to confer optimal beneficial effects. The present review focuses on the general functional, safety, and technological screening criteria for selection of ideal Bacillus probiotics as feed supplements as well as their mechanism of action and beneficial effects on monogastric animals for improving production performance and health status. Supplementary Information The online version contains supplementary material available at 10.1007/s12602-022-09909-5.

Recent studies have evidenced exogenous enzymes as nutritional tool to elaborate low-polluting aquafeeds. This study aimed to evaluate effects of enzyme complex (EC) on apparent digestibility coefficients (ADC) of energy and nutrients, including amino acids, as well nitrogen (N) and phosphorus (P) loss in pre-growout Nile tilapia (Oreochromis niloticus). Diets without EC (Control) or with EC supplemented at 225 (EC225) or 450 mg/kg diet (EC450) and fed to Nile tilapia (n = 135, 150 ± 20 g) distributed in an entirely randomized design of three treatments and three replicates of 15 fish each. Fish fed diet EC450 showed higher digestibility of energy, protein, amino acids and mineral, and increased digestible energy (DE; +221.25 kcal/kg diet), digestible protein (DP; +10.54 g/kg diet) contents of diets, whereas reduced N (−23.82%) and P (−18.46%) loss relative to fish fed diet control. This study evidenced that EC at 450 mg/kg diet optimizes the ADC of multiple nutrients, including amino acids, and identified its potential to enhance the nutritive value of feeds and elaborate sustainable feeds for Nile tilapia. RESUMO Estudos recentes têm evidenciado enzimas exógenas como ferramenta nutricional para elaboração de dietas aquáticas pouco poluentes. Este estudo teve como objetivo avaliar os efeitos de um complexo enzimático (CE) nos coeficientes de digestibilidade aparente (CDA) de energia e nutrientes, incluindo aminoácidos, bem como perda de nitrogênio (N) e fósforo (P) em tilápia-do-nilo, Oreochromis niloticus em crescimento. Dietas sem CE (Controle) ou com EC suplementado em 225 (CE225) ou 450 mg/kg dieta (CE450) foram fornecidas para tilápias do Nilo (n = 135, 150 ± 20 g) distribuídas em um desenho inteiramente aleatório de três tratamentos e três repetições de 15 peixes cada. Peixes alimentados com a dieta EC450 apresentaram maior digestibilidade da energia, proteína, aminoácidos e minerais, e aumento nos conteúdos de energia digestível (ED; +221,25 kcal/kg dieta) e proteína digestível (PD; +10,54 g/kg dieta) das dietas, enquanto houve redução na perda de N (−23,82%) e P (−18,46%) em relação aos peixes alimentados com a dieta de controle. Este estudo evidenciou que o CE a 450 mg/kg de dieta otimiza o CDA de múltiplos nutrientes, incluindo aminoácidos e identificou seu potencial para melhorar o valor nutritivo dos alimentos, assim como elaborar dietas sustentáveis para tilápias do Nilo.

A total of 160 growing pigs (24.69 ± 1.89 kg) were randomly assigned to one of four treatments in a 2 × 2 factorial design with two different levels of nutrient density diet with or without 0.3% probiotic (Lactobacillus plantarum). Each treatment had eight replicates with five pigs (three gilts and two barrows) per pen. At the end of the trial, pigs fed 0.3% probiotic supplement had significantly increased body weight, whereas the average daily gain and gain to feed ratio was significantly increased in both probiotic and high-nutrient density (HD) diet. The nutrient digestibility of dry matter was significantly increased in pigs fed HD diet, whereas nitrogen and gross energy digestibility and blood characters immunoglobulin and lymphocyte counts were significantly increased in both HD and probiotic groups. Inclusion of HD diet with 0.3% probiotic significantly decreased NH3 and H2S gas emission. Moreover, nitrogen and energy showed a significant interaction between probiotic and density diet. In summary, dietary probiotics with HD diet increased the growth performance, nutrient digestibility, blood profile, and reduced gas emission. We suggest that 0.3% probiotic with HD diet could serve as an alternative feed additive to enhance the growth performance of growing pigs.

The purpose of this research was to determine the improvement of the carcass quality of broiler ducks with the addition of meniran extract as a feed additive. This is based on the fluctuating price of feed 70 – 80% is part of the operational costs and the prohibition on the use of antibiotics requires alternative feed additives that are safe for human consumption. This research was carried out in Juni – August 2021 in Dayu Village, Nglegok District Blitar Regency. The method used is experimental with 4 treatments and 3 replications. The design used is Completely Randomized Design. The result showed that giving meniran extract as a feed additive could improve the carcass quality of broiler duck including carcass weight and carcass percentage and dit not have a significant effect on slaughter weight. It is recommended to use meniran plan extract level 3% to improve the carcass quality of broiler ducks.

Background and Aim: In the post-antibiotic era, consumer demand for healthy and safe meats has prompted poultry producers to seek alternative effective feed additives. This study aimed to investigate the effects of a novel natural feed additive based on a mixture of Averrhoa bilimbi L. fruit filtrate, wheat bran, and Saccharomyces cerevisiae on the growth rate, internal organ weight, and breast meat characteristics of broilers. Materials and Methods: A total of 280 1-day-old chicks were divided into one control (CNTRL; feed without additives) and three treatment groups: NOV25, feed with 2.5 g/kg novel additive; NOV50, feed with 5.0 g/kg novel additive; and NOV100, feed with 10 g/kg novel additive. The body weight (BW), feed intake (FI), and feed conversion ratio (FCR) were measured weekly. On day 35, the chickens from each group were slaughtered, and their internal organs and breast meat samples were collected. Results: The BW of broilers in NOV100 was greater (p=0.016) than that in the other groups. The FCRs in the treatments groups were lower (p

The purpose of the research is to develop an effective feed additive with an optimal content of raw protein in combination with flavoring additives for ducklings, which allows to increase zoo technical and economic indicators with broiler technology of fattening young animals. Research methods. A scientific and production experiment was conducted on three groups of analog ducklings from a daily age to 49 days old. The studied indicators are: the dynamics of live weight by fattening phases, absolute and average daily growth, the safety of young animals, feed conversion, slaughter indicators, the European fattening efficiency indicator, the profitability of meat production. Results. The use of compound feeds with an optimal level of protein content in combination with flavoring additives prepared on the basis of developed feed additives intended for fattening broiler ducklings in the fattening phases contributes to an increase in: live weight by 11.4 %, the safety of young animals by 3 %, feed consumption and conversion by 3.9 and 7.6 %, slaughter yield of 1.1 %, the European fattening efficiency indicator from 246 to 308 units and the profitability of meat production by 24.5 %. Scientific novelty. For the first time, a complex feed additive has been developed, which, according to the age periods of fattening, includes three varieties: “Prestart”, intended for intensive fattening of young animals from daily to 10 days of age inclusive, “Start” for ducklings from 11 to 21 days and “Finish” from 22 to 49 days. The rate of introduction of the additive is 30 % of the mass of mixed feed. The composition of the supplement includes high-protein feeds, premixes, amino acids and flavoring substances (monosodium glutamate, acetic and citric acids, a mixture of phytogenics: oregano, cinnamon, red pepper).

Chlorella shows a wide spectrum of biological activity, in particular, it exhibits a pronounced antioxidant activity and demonstrates anti-inflammatory, antitumor and antiviral properties. A number of research works have been devoted to studying feed advantages of this unicellular green algae when used in the diets of livestock animals, but the possibility of including different Chlorella species in the diet of primates has not been practically studied. The aim of this work was to assess the possibility of replacing high-protein animal and vegetable feeds with Chlorella , to calculate the digestibility coefficients for the diet nutrients and the effect of algal dry and suspension forms on hematological and serum biochemical parameters in male rhesus monkeys. The data obtained during the experiment indicate that the inclusion of Chlorella in the diet both in the dry form and cell suspension improves nutrient digestibility. Thus, the digestibility of crude protein in the animals receiving algae suspension increased by 4.18% ( p < 0.05), that of crude fat – by 4.70% ( p < 0.01), crude fiber – by 4.14% ( p < 0.05) and crude ash – by 12.32% ( p < 0.001). The digestibility coefficients of crude protein in the primates receiving compound feed supplemented with Chlorella powder were higher by 6.83% ( p < 0.001), those of crude fiber – by 4.78% ( p < 0.05) and crude ash – by 18.93% ( p < 0.001) The hematological study results indicate the absence of side effects from long-term Chlorella consumption by primates. The introduction of dry Chlorella into the diet increased blood glucose levels to the upper limit of the control values, while Chlorella suspension did not produce this effect. Thus, Chlorella can be successfully used as a component of a balanced laboratory diet for primates or as a feed additive.

Laboratory and field experiment on use of the feed additive based on Bacillus subtilis endo- and exometabolites for the cows in different physiological periods are described in the paper. The feed additive impact on main body systems of the tested cows (n = 30), milk production parameters, growth rate of the calves (n = 18) born to the said cows were examined. The feed additive was added to the diets for the cows of test groups, 15 g per cow. The feed additive was found to have a positive effect on immunohematological and metabolic processes in postpartum cows. Neutrophils’ phagocytic activity increased by 12.5% and 14.6% in the animals of test group 1 and test group 2, respectively, as compared to that one in control animals (42.8 ± 1.9%). Neutrophil absorbency increased by 2.5 times, 3.2 times and 2.1 times in the animals of test group 1, test group 2 and control group, respectively. The proportion of T-lymphocytes in blood of animals in test group 1 and test group 2 was 44.5 and 48.9%, respectively, proportion of T-lymphocytes in blood of control animals equaled to 37.5%. Trend for increase in total protein concentration in cow sera owing to increase in albumin fraction was observed in postpartum period: it was 72.91 ± 3.45 g/l in test group 1; 75.54 ± 4.12 g/l in test group 2; 70.95 ± 4.25 g/l in control group. Average daily milk yield in cows of test group 1, test group 2 and control group for the 150 days of lactation was 24.50 ± 1.86 kg; 25.33 ± 1.45 kg and 22.75 ± 4.41 kg, respectively. Higher growth rate was reported for the calves born to the cows received the diet supplemented with the said feed additive. Heifers of test group 1 and test group 2 have reached body weight of 193.51 ± 5.76 and 195.33 ± 3.76 kg and in control group – of 187.33 ± 4.98 kg within 6 months. Feed additive based on endo- and exometabolites of Bacillus subtilis is recommended for cow diets for highly productive dairy herd creation and food-producing animal welfare maintenance.

Riboflavin is a feed additive, food additive, and clinical drug, with a significant annual demand of nearly 8000 tons. Fermentation using recombinant Bacillus subtilis is currently one of the most important industrial production method for riboflavin. First, a suitable medium was selected and the expression of the ureABC operon was modified. The ykgB gene was overexpressed in B. subtilis RX10, the production of the derivative strain RX20 was increased to 4.61 g l-1 riboflavin, and the yield was increased to 52 mg riboflavin g-1 glucose. The relative transcription level of pyr operon in RX20 was reduced to 71%, the production of the derivative strain RX21 was increased to 5.82 g l-1 riboflavin, and the yield was 76 mg riboflavin g-1 glucose. The start codon of the pyrE gene in RX21 was modified to "TTG", the production of the derivative strain RX22 was increased to 7.01 g l-1 riboflavin, and the yield was 89 mg riboflavin g-1 glucose. These results indicated that overexpression of the ykgB gene and reduction of the metabolic flux of de novo synthesis of pyrimidine nucleotides were beneficial to the synthesis of riboflavin.

Catfish which is an important component of global freshwater aquaculture production is also prone to a wide range of diseases resulting in economic losses. Improvement of the immune status of catfish is a promising preventive approach to control disease. The purpose of this study was to determine the effect of fermented earthworms as a feed additive on the non-specific immune response of walking catfish (Clarias gariepinus). Earthworms were fermented by inoculation with a bacterial mixture of Bacillus spp. and Lactococcus raffinolactis. The study featured a completely randomized design, with five treatments in quadruplicate. The various treatments consisted of fermented earthworms supplemented (FE) at at 0%, 2.5%, 5%, 7.5%, and 10% (w/w). Catfish with an average weight of 15 g were stocked at a density of 50 fish per 50 L/tank, reared for 2 months, and fed thrice a day at a feeding rate of 3% of total boby weight. The parameters observed included phagocytic activity (PA), phagocytic index (PI), extracellular respiratory burst, leucocyte differentiation, super oxide dismutase (SOD), natural agglutination, leucocrit, and hematocrit. Results showed that that FE supplemented at 2.5% significantly increased the respiratory burst activity, hematocrit, PA, PI, SOD, and leucocyte differentiation (P

Context Broilers are prone to immunological stress when subjected to unsuitable environmental conditions (such as virus attack, nutrient deficiency and high stocking density), lowering immunity and resulting in inflammatory bowel diseases. The herb-feed additive Artemisia argyi has been applied in poultry production, and its extract may improve small intestinal immune capacity. Aims This study was conducted to investigate the effect of A. argyi alcohol extract (AAAE) on growth performance and blood biochemical and small intestinal immune markers of broilers challenged with a proinflammatory substance, lipopolysaccharide (LPS). The study also examined possible mechanisms of action, and aimed to clarify whether AAAE could be applied as a feed additive. Methods In total, 192 one-day-old Arbor Acres broilers were allocated to four groups following a 2 × 2 factorial arrangement (including six replicates with eight birds per replicate) with two dietary AAAE rates (0 or 750 mg/kg) and two immune stress treatments (LPS or saline injection). Blood and small intestine were sampled on Days 21 and 35. Key results Dietary AAAE alleviated the LPS-induced decrease in bodyweight, average daily gain and average daily feed intake, and mitigated the elevated serum alanine aminotransferase, triglyceride, low-density lipoprotein cholesterol, adrenocorticotropic hormone and corticosterone concentrations at Day 21 and/or Day 35 in LPS-challenged broilers. AAAE significantly (P

Relevance. Modern livestock breeders face an important task of producing organic products, in Russia this is regulated by the Federal Law “On Organic Products” (from 01.01.2020), it allows producers to enter in the state register and mark the products with the “organic” sign. There is a problem of deterioration of the quality of milk, low content of fat, protein, SOMO, deficiency of macro-and microelements, vitamins, which is associated with a violation of the proper feeding of animals. The use of innovative technologies for the activation and modification of silicon-containing minerals (diatomite and zeolite clinoptilolite) enhances their properties. This makes it possible to use them as an adsorbent, an ion exchanger and a source of readily available silicon and other mineral elements to produce high-quality organic products.Methods. To achieve this goal, in the Ulyanovsk region we organized a production experience in the conditions of a dairy farm of “Agrofirma Tetyushskoe” for a duration of 100 days. Three groups of 50 cows were formed: 1st — control, received only the basic diet (ОR), 2nd — experimental (ОR+ supplement based on modified zeolite enriched with amino acids), 3rd — experimental (ОR+ supplement based on modified diatomite enriched with amino acids). The supplement was given once a day, in the morning in a mixture with mixed feed, the input rate was 250 g/head/day. For the physiological experiment, 5 analog cows were selected in a group. To enrich the minerals, a complex of plant-derived amino acids of high purity and biological activity was used.Results The intake of additives based on silicon-containing natural minerals (zeolite and diatomite), processed with innovative technologies and enriched with plant-based amino acids, increases the level of animal productivity and ensures the yield of organic products high-quality. It has a prolonging effect.

Increasing the efficiency of feed with the use of different feed additives is becoming a particularly common method in aquaculture. Sangrovit Extra is a plant feed additive for increasing the productivity of farm animals due to the anti-infl ammatory and immunomodulatory properties of its components. The purpose of the research was to evaluate the effectiveness of the use of the Sangrovit Extra additive as part of trout compound feed in feeding rainbow trout when rearing in cages on the warm waters of the regional hydroelectric power plant. The object of research was individuals of rainbow trout. The introduction of the Sangrovit Extra feed additive in the amount of 400 mg/kg into the main diet of fish best contributed to the increase in the average daily gain of trout in cages by 0,48 g compared to the control group. The use of the studied additive contributed to the increase in the assimilation of feed, which was manifested in the indicator of its consumtion. When trout weighing 200–300 g were grown on the main diet (without additives), feed consumption per 1 kg of fish growth amounted to 1,26 kg, whereas when introduced into the feed in the amount of 100 mg/kg, 400 and 700 mg/kg, the efficiency of feed use increased by 9 %, 11 and 5 %, respectively. When rearing larger trout (1,2–1,5 kg), feed consumptions when using the additive in the amount of 400 mg/kg decreased from 1,47 to 1,36 kg, i.e. by 10,8 %. The use of the Sangrovit Extra additive when rearing commercial rainbow trout in cages on warm waters has shown the economic feasibility of its use, especially at the dosage of 400 mg/kg of feed. The level of profitability for rearing trout weighing 0,3 kg was 18,6 % higher, and for fish weighing 0,5 kg by 12,7 % compared to the control group.

It is known that mineral additives play an important role in increasing the productivity and efficiency of the animal husbanry industry as a whole. The state of the body can be assessed by the content of mineral substances in a blood test. The purpose of the work was to study changes in morphological and biochemical blood parameters in first-calf heifers during the period of increasing the milk yield when they are fed a complex mineral and vitamin feed additive (CMVFA). The study of the influence of feeding the complex mineral and vitamin feed additive in the ration to first-calf heifers during the the period of increasing the milk yield on morphological and biochemical blood parameters has been carried out. The experiments were carried out in 2019–2020 in the OOO Breeding Farm “Valiev” in the Dyurtyulinsky district in the Republic of Bashkortostan on 3 groups of first-calf heifers of Black-and-White breed per 15 heads in each selected by the method of pairs of analogues. The animals of the control group received the main ration (MR) adopted on the farm, the 1st experimental group MR+120 g/head CMVFA according to the recipe No. 1, the 2nd experimental group MR+120 g/head CMVFA according to the recipe No. 2. In the ration of animals of the control group there was a deficiency of basic macro- and microelements. The use of CMVFA as part of the ration of first-calf heifers during the period of increasing the milk yield had a positive effect on the morphological and biochemical parameters of blood. Studies have shown that the use of CMVFA in the rations of first-calf heifers provided the need for minerals and vitamins, accelerating metabolic processes in the body during the most stressful period of lactation, the period of increasing the milk yield. According to the results of biochemical blood parameters, it is possible to esimate the improvement of protein, carbohydrate and mineral and vitamin nutrition in the first-cakf heifers of the experimental groups during the period of increasing the milk yield, which showed their genetic potential more fully and used nutrients more effectively for milk synthesis.

Objective The effects of docusate (DOC) supplementation on feed intake and digestion of cattle were studied. Materials and Methods Four Simmental × Xinjiang Brown nonpregnant female cattle, with a BW of 248.9 ± 9.4 kg and a permanent rumen cannula, were assigned to 4 treatments: dietary supplementation of DOC at 0 (the control), 0.8, 1.2, and 1.6 g/kg of DM diet in a 4 × 4 Latin square design. Each period consisted of 16 d for adaptation followed by 6 d for sample collection (feed, feces, and mixture of feces + urine) and measurements. All animals were fed individually with the same basal diet. Results and Discussion Supplementation with DOC had a quadratic effect on voluntary feed intake (VFI); VFA concentration in rumen fluid; amounts of digested nutrients; and the retention of nitrogen (N), calcium (Ca), and phosphorus (P) compared with the control. The highest increment values were 39.5% for VFI, 14.9% for VFA, and 9.1% for N retention at a DOC dose of 1.2 g/kg of diet. The ruminal ammonia concentration declined linearly with increasing DOC dose. Docusate supplementation decreased, in a quadratic manner, the digestibility of dietary components, likely due to the increased VFI. It was concluded that DOC fed at 1.2 g/kg of diet stimulated VFI and digestion in cattle. Implications and Applications Supplementation with DOC at the appropriate dose can stimulate feed intake and digestion in cattle, and DOC could be used as a feed additive for ruminants.

This aim of study was to determined effect of red ginger flour (Zingiber Officinale Var Rubrum) as herbal feed additive in Broiler Chiken on feed comsumption, daily body weight gain and feed conversion ratio.The research was conducted on September – Oktober 2020 at Gisting Atas Village, Gisting District, Tanggamus Regency, Lampung. The experiment used 80 unisex Broiler aged 1 week. They were feed a ration with control ration + 0% red ginger flour (P0), control ration + 0,25% red ginger flour (P1), control ration + 0,5% red ginger flour (P2), control ratio + 0,75% red ginger flour (P3) during 20 days. The control ration containing 21% crude protein and 3108 kkal/kg metabolism energy. The study used a quantitative methods with completely randomized design with 4 treatments and 4 replications. The results showed that the ration had no effect (P> 0,05) on feed comsumption, daily weight gain and conversion ratio. The conclution of the research showed that the addition of level red ginger flour (Zingiber Officinale Var Rubru) until 0,75% as herbal feed additive in Broiler Chiken no effect on feed comsumption, daily body weight gain and feed conversion ratio. Keywords: Red ginger flour, Broiler, Feed Comsumption, Daily Weight Gain, Feed Conversion Ratio.

Context Reducing egg cholesterol has been a major goal of many researchers over four decades. Monacolin K, a 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase inhibitor, identical to the active ingredient in the prescription statin drug lovastatin, could be a promising feed supplement for layers. Aims To determine the effects of high monacolin K red yeast rice (RYR) supplement on feed intake, egg production, egg cholesterol content, plasma lipid and enzyme metabolites in laying hens. Methods Hyline brown layers were fed RYR supplement at 0, 0.2, 0.4, 0.8 and 1.6% of the diet for 6 weeks. Total and daily feed intake, egg production, egg weights and feed conversion ratio between the RYR treated groups and the control were compiled. Egg cholesterol and egg quality at the 3rd and 6th week of the feeding trial were analysed. Plasma lipids and enzymes were sampled towards the end of the trial. Key results Egg cholesterol were significantly lower (P < 0.05) in the 0.8 and 1.6% RYR supplemented group. Supplementation with 0.8% RYR resulted in 11.16% reduction in egg cholesterol at the 3rd week with no further improvements with longer feeding. There were no significant differences (P > 0.05) in total and daily feed intake, egg production, egg weights and feed conversion ratio (FCR) between the RYR treated and control group. Total plasma cholesterol and triglycerides were significantly lower (P < 0.05) in the 0.8 and 1.6% RYR supplemented group when compared with the control group. RYR supplementation exert no significant differences (P > 0.05) in plasma enzyme levels. Conclusions The present study suggests that RYR supplementation of 0.8% resulted in egg cholesterol reduction by 11.16% with no adverse effects on laying hens. Implications The feeding trial showed that RYR supplement can be used as a feed additive in layer diets to lower egg cholesterol, plasma cholesterol and triglycerides with no adverse effects on layer egg production and plasma enzymes over the feeding period.

The yeast Yarrowia lipolytica has been industrially adopted for docosahexaenoic acid and eicosapentaenoic acid production under good manufacturing practices over 2 decades. In recent years, it has claimed attention for novel biotechnological applications, such as a functional feed additive for animals. Studies have demonstrated that this yeast is safe and has probiotic and nutritional properties for mammals, birds, fish, crustaceans, and molluscs. Animals fed Y. lipolytica enhanced productive and immune parameters, as well as modulated microbiome, fatty acid composition, and biochemical profiles. Additionally, some Y. lipolytica-derived compounds have improved productive performance, immune status, and disease resistance in animals. Therefore, the aim of this review is to identify and discuss research advances on the potential use of this yeast for animals of economic interest. Challenges, opportunities, and trends were identified and envisioned in the near future for this industrially produced yeast. KEY POINTS: • Yarrowia lipolytica has probiotic and nutritional effects in animals. • Lipase2, EPA, and β-glucan from Y. lipolytica have health benefits for animals. • Y. lipolytica is envisioned in terrestrial and aquatic animal production systems.

Bentonite clays exhibit high adsorptive capacity for contaminants and is frequently used as a feed additive to reduce the bioavailability and thus the toxicity of several mycotoxins. Zearalenone (ZEN) is a secondary Fusarium toxic metabolite that can contaminate a wide range of food- and feedstuff. Since organophilic treatments is known to increase the adsorption capacity of bentonites, the aim of study was to evaluate and compare the ability of natural bentonite and bentonite treated with BAC302 to protect against ZEN-induced cytotoxicity in the epithelial colorectal adenocarcinoma (Caco-2) and human leukemia monocytic (THP-1) cell lines. The two materials were not toxic to the cell lines at lower concentrations. Furthermore, the results indicate that the two materials protect the Caco-2 and THP-1 cells against ZEN-induced cytotoxicity, probably by extracellular adsorption of ZEN. The tested natural bentonite shows potential for in vivo testing to evaluate if it is suitable for intoxication in ZEN contaminated animal feeds.

The article presents studies on the evaluation of the commercial qualities of the Lena sturgeon when using the feed additive "Reasil ® Humic Health" in its diet. The obtained data allow us to conclude that the addition of the Reasil ® Humic Health feed additive based on unmodified microporous humic acids from leonardite to the granulated compound feed has a positive effect on the growth and development of the Lena sturgeon and increases their yield of edible body parts by 1.74 %, while not adversely affecting the anatomical state of the internal organs of the fish and contributing to the development of the internal organs of the Lena sturgeon. The results of the chemical analysis allow us to say about the intensive metabolism in the body of fish that received a feed additive and the deposition of a larger amount of dry matter in their muscle tissue.

This article presents results of the influence of three probiotic feed additive of various microbiological composition: Bacillus subtilis (VKPM B-2335); B. subtilis (OZ-2 VKPM-11966) + Bacillus amyloliquefaciens (OZ-3 VKPM-11967); Lactobacillus acidophilus (VKPM B-3235) on the growth and histology of the organs of the gastrointestinal tract of juvenile Oncorhynchus mykiss by morphometric parameters. These probiotic bacteria are the most commonly used in aquaculture. The effect of the probiotic feed additive led to the increase in fish growth and influenced different sections of the gastrointestinal tract. The biggest change was found in the mid intestine and the reliable difference compared with the control diet was obtained at the following parameters: lamina propria width, intraepithelial lymphocytes number of prismatic epithelium and goblet cells area. The changes in the pyloric appendages were less obvious but reported as playing an important functional role in digestion. The liver preserved normal functional structure in all series of the experiment except for the group with L. acidophilus, where hepatocyte small-drop vacuolization was observed. That might be connected with the change of the digest activity resulting from a decrease in secretory activity of the intestinal exocrinocytes. The use of all probiotic feed additives led to a similar change in morphometric parameters in all groups, which suggests a decrease in the immune response.

The search for new, biotechnologically useful yeast strains has been carried out in many research centers in the world. Sporobolomyces and Sporidiobolus are examples of such useful yeasts, that can be used as a source of many valuable metabolites in industries. This article describes the modern taxonomy of these yeasts, which resulted from many years of research, including both classical microbiology and genetic analyses. Subsequently, the paper presents a review of scientific studies on the biosynthesis of various extracellular and intracellular metabolites produced by Sporobolomyces and Sporidiobolus yeasts, which are of great importance in the contemporary food, feed, and pharmaceutical industries. Such metabolites include exopolysaccharides, lipids, carotenoids, enzymes, and γ-decalactone. Aiming at developing a sustainable circular bioeconomy, this study considers two directions of use of these yeasts, i.e., as a feed additive and as an antagonist in the biocontrol of plant materials. This article is one of the first to organize the knowledge collected from published studies and present the contemporary scientific achievements and prospects for the biotechnological use of Sporobolomyces and Sporidiobolus yeasts.

The survey to measure the impact of antistress pharmacologic agents, particularly the SPAO-complex product along with the SPAO-KD feed additive, on the immune status of chickens to prevent tenosynovitis was carried out. In order to conduct the surveys, the chickens were allocated into three groups of 32 000 heads each to build the parent flock. Chickens in the first group served as the control. The specimens in the second test group were provided with the SPAO-KD feed additive in the mixed fodder. The poultry in the third test group were provided with the SPAO-complex pharmocological-composition product with water. The dietary preventive agents were used at a dose of 185 mg/kg body weight for 5 days in the reference live vaccine used for vaccination: 3 days before vaccinations, on the day of vaccination, and 1 day after vaccination. Poultry immunization against reovirus tenosynovitis was carried out according to the technology for population vaccination. A dried live vaccine against avian reovirus tenosynovitis was used to vaccinate the chickens at the age of 1 and 4 weeks. It was produced from an attenuated strain 1133 of avian reovirus isolated from tenosynovitis. The seroconversion rates in quantitative and qualitative manners in the reference test agents were assessed 7 and 14 days after the first vaccination and 14 and 21 days after the second vaccination. The dietary SPAO-KD feed additive and the water-soluble SPAO-complex pharmacological product contributed to an increase in the levels of postvaccination antibodies 1.6- and 2.6-fold, respectively, and the vaccination indices 1.6- and 3.5-fold, respectively. The SPAO complex product contributed to reducing the coefficient of variation of postvaccination antibodies by 25.7%. Taking antistress therapies under the effects of immunologic load factors provided the poultry welfare benefit and improved the production of day-old chicks and the poultry live weight gain by 0.79–3.5, 0.55–4.22, and 0.88–6.44%, respectively.

Animal feed production is an important step of the food animal production chain in a farm-to-table model. The contamination of raw ingredients with foodborne pathogens in feed production remains as an important safety issue where pathogens may spread into food animals to cause illnesses in humans when affected food animals are consumed. In the present study, we aimed to examine the quality and microbial contamination of fish meal and to investigate the effectiveness of the organic acid-based antimicrobial agent SALTEC 514TM against Salmonella to prevent bacterial contamination in fish meal. Fish meal samples (n = 4) collected from feed mills at different locations were analyzed for protein and total volatile basic nitrogen (TVBN) content to assess their nutritional value and freshness, and its microbiological quality. The protein and TVBN content ranged from 53.2 ± 3.1 to 67.5 ± 2.3 g/100 g and 73.8 ± 4.5 to 100.4 ± 11.2 mg/100 g meal, respectively. Total plate count of the fish meal samples ranged from 2.0 ± 0.3 to 4.5 ± 0.5 log units, whereas suspected foodborne bacteria, Escherichia coli and Salmonella, were not detected in all samples. Fish meal samples were artificially contaminated (day 0) and re-challenged (day 30 and 90) with Salmonella Enteritidis (3 log CFU/g) to test for the effectiveness of SALTEC 514TM, an organic acid-based antimicrobial formulation, in preventing Salmonella contamination and recontamination during storage. SALTEC 514TM, when applied at three different doses, was found to reduce the number of Salmonella in monitored samples after one day of storage. A low dose of 0.5 kg/ton SALTEC 514TM prevented Salmonella recontamination from occurring in fish meal samples stored for 37 days. In medium (1.0 kg/ton) and high doses (3.0 kg/ton), applications of SALTEC 514TM prevented the Salmonella recontamination for a maximum storage duration of 97 days. The application of SALTEC 514TM in fish meal and/or other feed ingredients may prove to be a safe alternative to reduce the microbial load, especially of foodborne-related microorganisms, to contribute to feed and food safety.

Research has been carried out to study the influence of "ProBioKorm Uz", a biologically active feed additive, on the development and physiological characteristics of rabbits of ‘New Zealand’ breed. During the experiment, 2 experimental and a control groups were formed. Adding "ProBioKorm Uz" to the main feed at the rate of 1% and 2% had an increase in experimental group rabbits’ live weight. Average live weight of them was higher by 5,7 %-3.8% than in the control group. Feed units composed 1.59%, dry matter 0.56 kg (3.61%) and crude protein 0.11 kg (3.59%) compared with the control group. Rabbits of experimental groups had higher indicators of erythrocytes concentration by 0.34-1.00 1012/l (10.34-19.53%), leukocytes - by 0.370-0.46 109/l (5.66-8, 06%), as well as a high level of hemoglobin by 7.42-11.08 g/l (6.81-10.08%) and total protein - by 2.40-4.48 (3.64-6, 42%).

This study was conducted to determine the effect of Ocimum gratissimum as a phytogenic feed additive (PFA) on growth performance, carcass, and blood profile of broiler chicken. One hundred and sixty day old broiler chickens were used for the study. Four diets were formulated to incorporate Ocimum gratissimum at 0, 50, 100 and 150 g per 100 kg of feed to form T1, T2, T3, and T4 respectively. The birds were divided into four groups of forty birds and each group was assigned one of the diets in a completely randomized design. Each group was further divided into four replicates of 10 birds each. Feed and water were supplied ad libitum and data collected were statistically analyzed. The result obtained showed significant (p0.05) at both the starter and the finisher phases. Significant (p0.05) by the treatments. It can be concluded that Ocimum gratissimum could be added to broiler diet at 150 g/100kg as feed additive without any deleterious effect on the growth performance, carcass, and blood profiles of the birds.

Normalization of the bacterial background of the gastrointestinal tract and the reaction of the environment largely contributes to the growth and development of poultry in accordance with the standard of cross. The addition of the prebiotic Salmocil F to the diet of replacement young poultry at the dose of 0,6 kg, 0,8 and 1,0 kg/t of compound feed allowed the poultry to grow to 18 weeks of age with a live weight of 1836,2 kg, 1915,4 and 1885,0 kg, respectively, which was higher than the control group by 0,7 %, 5,0 and 3,3 %, and the uniformity of the group by 8,0–9,0 %. The prebiotic Salmocil F contributed to the better development of the replacement young poultry such as muscularization of the pectoral muscle, its tone and physical shape. The prebiotic Salmocil F excluded the reason for the culling of poultry in the experimental group for toxicosis and intestinal intussusception, increasing the livability of livestock in the experimental group with the low dosage by 3,0 %, with medium and high by 4,0 %. The low dose of prebiotic in the diet of poultry of the 2nd experimental group in comparison with the 1st control group reduced expenditures of feed by 5,5 %, the average rate of input in the 3rd experimental group by 6,7 %, the high dose in the diet of chicks of the 4th experimental group by 6,1 %. The feed-conversion efficiency for receiving replacement young poultry per each 100 kg of feed fed in the group with the dosage of 0,6 kg in comparison with the control group was higher by 6,0 %, with the increase to 0,8 kg by 7,2 and with the addition of 1,0 kg/t of feed by 4,1 %, and in value terms by 5,4 %, 6,5 and 5,6 %, respectively. More optimal is the dosage of 0,8 kg of the prebiotic Salmocil F per 1 ton of compound feed.

Currently, there is a rapid development of commercial salmon farming in Russia, which has grown almost 3 times in terms of production since 2013 and exceeded 116 thousand tons in 2020. In turn, this requires the appropriate development of the feed base and the development of methods for the effective use of fairly expensive fi sh feed. From these positions the involvement of the feed additive “Vinivet”, which is a waste of beekeeping in the feed production for fi sh as a protein additive is relevant. The feed additive “Vinivet” contains bee products such as bee bread and slumgum, a significant complex of vitamins, amino acids, macro- and microelements. The purpose of the work was to assess the influence of the feed additive “Vinivet” on the livability of juveniles, growth rate and feed consumption of rainbow trout of Steelhead salmon breed when growing in a recirculating aquaculture system. For the experiment juveniles with an average juvenile weight of 2,37±0,72 g have been selected, from which the control and 3 experimental groups have been formed. The control group has received a standard starting compound feed as the main diet, the 1st experimental group in addition to the main diet has received the additive “Vinivet” in the amount of 2,5 % of the mass of the main diet, the 2nd experimental group has received 5,0 % of the additive, and the 3rd experimental group in the first week received 2,5 % of the additive, and from the second week of studies the dose of the additive was increased to 5 %. The conducted studies have shown that the inclusion of the feed additive “Vinivet” in the feed when growing juveniles of Steelhead salmon is most advisable by introducing it at the dosage of 2,5 % of the mass of the main diet in the first week of cultivation, followed by the increase in the dose to 5,0 %, which allows achieving the highest fi sh productivity of pools in the recirculating aquaculture system compared to all variants of the experiment and by 24 % compared to the control group.

The present study investigated the influence of the quail diet polluted with aflatoxin B1 (AFB1) and its detoxification by using clay as a feed additive on the growth performance and some blood biochemical components of growing Japanese quail with reference to sex. A total number of 120 Japanese quail chicks (1 week old), was randomly divided into 10 groups (24 chicks/ group). A 5 × 2 factorial arrangement experiment was performed and included five levels of AFB1 (0 ppm, 1 mg/kg AFB1, 1 mg/kg AFB1 + 1% clay, 2 mg/kg AFB1 and 2 mg/kg AFB1 + 1% clay) and two sexes. Birds fed with aflatoxin free diet had significantly (P ≤ 0.05 and 0.01) higher final live body weight, weight gain and lower mortality rate than the other groups. Addition of 1% clay significantly (P ≤ 0.05 and 0.01) improved the growth performance traits and diminished aflatoxin effect when compared to groups without the addition of clay. Obtained results indicated significant (P ≤ 0.05) differences between the two sexes in their response to aflatoxicosis in the final live body weight and weight gain. Our results showed significant (P ≤ 0.01) changes in all blood biochemicals (total protein, albumin, globulin, total cholesterol, creatinine, uric acid) and activities of serum enzymes studied due to the toxicity of AFB1. Conclusively, the consumption of polluted diets with AFB1 caused deleterious effects on the growth performance and blood biochemicals components of Japanese quail, while dietary addition of natural clay to the diet of growing Japanese quail caused beneficial effects.

The aim of this study was to evaluate the detoxification of aflatoxin B1 (AFB1) in vitro and in broiler chickens using a triple-action compound mycotoxin detoxifier (CMD). Response surface methodol...

The effect of the feed additive based on humic acids "Reasil® Humic Vet" on the body of lactating cows has been studied. It has been shown that in the process of lactation, the intensity of metabolic processes increases, as evidenced by the blood counts of cows in the experimental group that were fed with "Reasil® Humic Vet". The content of hemoglobin was higher by 5.5%, erythrocytes - by 6.6%, alkaline reserve - by 5.2% (P

This research aimed to evaluate the nutrient digestibility and the growth performance of broilers fed a different type of diet (TD) and feed additives (FA). A total of 264 day-old chicks (male, Lohmann) were randomly distributed into 24 pens (11 birds/pen). The experiment was designed by using a 2 x 4 factorial completely randomized design with the main factors TD and FA. The result shows that TD x FA interaction was significant (p0.05) nutrient digestibility (ND) and energy values (EV). Except for crude protein, FA did not affect (p>0.05) on ND and EV. The TD x FA interaction did not affect ND and EV. TD affected (p

A feeding trial was carried out for 56 days to ascertain the effects of Telfairia occidentalis (fluted pumpkin) as a feed additive on the haematological profile and nutrients value of Clarias gariepinus carcass. C. gariepinus fingerlings (mean body weight 9.6g) were randomly distributed in plastic bowls at nine fish per bowl in triplicates. Five diets designated as D1 to D5 with 40% crude protein containing 0, 50, 100, 150, and 200g/kg of T. occidentalis leaf powder were fed to C. gariepinus fingerlings at 5% body weight per day. The results showed that T. occidentalis additive significantly (P≤0.05) improved the haematological parameters of the fish. The mean value of white blood cells (WBC) count (μL) in the diets fed with T. occidentalis were significantly higher (D2-D5 : 870.20, 864.80, 840.30, 876.40, respectively) than the diet without T. occidentalis (D1 783.15). A similar trend was observed in PCV, MCV, MCHC, MCH, and platelets. However, HB D1 (control) had higher values than the diet containing T. occidentalis. Meanwhile, in carcass quality, there was an increase in the crude protein of all the fish but the group that consumed T. occidentalis had a higher carcass crude protein and lower lipid, while the control had a higher lipid value. Meanwhile, the initial values obtained before the feeding trials had lower crude protein and higher lipid value when compared with the fish that was fed with the experimental diet. In conclusion, T. occidentalis leaf meal can be utilized to improve the haematological parameters of the fish, with an increase in the WBC there is a high possibility of the fish to withstand pathogenic attacks because Blood is a major parameter that determines the physiological, immune , and nutritional status of an organism, also WBC are important blood constituents for defense against diseases, pathogens and unwanted foreign bodies in an organism.

A most widely used organoarsenical compound known as Roxarsone is used as chicken feed additive which consists of both arsenic (III) and arsenic (V) including many other forms of arsenic. The present study highlighted a comprehensive evaluation of chicken litter with respect to the leaching of arsenic under a different pH, contamination of agricultural soil, and accumulation of arsenic in different parts of Pigeon pea (Cajanus cajan). Study results revealed that arsenic leaching from chicken litter is highly pH dependent and alkaline pH is a favourable condition for leaching of arsenic from litter. Depthwise arsenic distribution results indicate litter-mediated arsenic distributed in different layers through leaching process. Moreover, a pot experiment was conducted to investigate the uptake, translocation and distribution of total arsenic in Pigeon pea (Cajanus cajan) using different doses of poultry litter. Pot experiment results revealed that maximum arsenic is accumulated in the shoot than root and leaf (p

Purpose: estimation of the possibility of using a mixture of feed additive "BioGrom SC" and the food antibiotic "Monenzin" for normalizing metabolic processes in highly productive cows in the transit period.Materials and methods. Studies were conducted because of the tribal plant of the Leningrad region. Two groups of animals were selected on the principle of analogs (experimental and control) 20 goals in each. The experimental group of cows 21 days before the calving and for 14 days after the calves were given a mixture of feed additive "BioGrom SC" and the food antibiotic "Monenzin". For the cottage additives, calcium carbonate (chalk) was used as a filler in the amount of 250 g with the addition of 10-15 g. Supplements "BioGrom SC" by 1 head. The mixture was prepared at the rate of 20 goals and a food antibiotic "Monenzin" was added to it at the rate of 100 mg per 1 kg of a mixture of feed additive with filler. The resulting mixture was fed by animals of the experimental group. All animal experimental and control groups take blood for 21 days before the hotel. The total protein, beta hydroxy acid, acetoacetic acid, alkaline blood reserve, calcium-phosphoric ratio, glucose is determined. At the end of the transit period on the 21st day of lactation, the blood of animal control and experimental groups was repeatedly investigated.Results. In the cows of the experimental group, in comparison with the cows of the control group at the end of the transit period, a decrease in such biochemical indicators was observed as beta hydroxy acid (a decrease to 0.84 ± 0.03 mmol / l), acetoacetic acid (decrease to 0.4 ± 0, 04 mmol / l). The animals of the experimental group decreased the content of the general protein (77.0 ± 0.08 g / l). Also, the data of the backup alkalinity of blood (acidose indicator) are within reference values. Calcium phosphorus ratio was also within the normal range.Conclusion. Introduction to the diet of a mixture of feed additive "BioGrom SC" and food antibiotic "Monenzin" normalize some biochemical blood indicators in cows in the transit period. At the same time, without correction of metabolic processes, cows are observed ketosis and acidosis, which can lead to premature disposal of animals from herd and as a result of economic losses. Thus, the use of the mixture is shown for the prevention and treatment of acidosis of the scar, metabolic acidosis, ketosis in cattle of the dairy direction of productivity.

This review was intended to elucidate the role of antioxidants in poultry feed formula on bird's ecophysiology. Naturally, many compounds have antioxidant features, including fat and water-soluble compounds, which can either be synthesized by the body or supplemented with a diet. The additive of antioxidants on poultry fodder is usually utilized in order to elevate the steadiness of feed. Nevertheless, some antioxidants are believed to have biological consequences in vivo. Oxidative stress clues to biological destruction, which in turn can disturb growth and production in farmstead animals. These Stressful factors are created reactive oxygen species. Therefore, the natural and synthetic antioxidants that add to animal feed are important to diminish the free radicals that are thought to unfavorably on growth, immune status, elevated lipid rancidity, and poor quality of meat. Supplementation of balanced feed with many ingredients including natural and synthetic antioxidants will improve the bird's performance and other physiological aspects by reducing the significant effects of free radicals. In conclusion, feed additive including antioxidants integrated with feed formula is beneficial and valuable to exclude and prevent the destructive effects of reactive oxygen species on physiological aspects of vital organs, maintenance of homeostasis, Repair and removal of harmed particles, activation of apoptosis and finally limit of mutagenesis.

In order to increase the meat productivity of broiler chickens, increase production volumes and reduce the prime-cost of poultry products, it is necessary not only to ensure balanced feeding and compliance with production technology but also to include in the poultry diet non-traditional additives, biologically active substances that contribute to the normalization of metabolic processes and improve the digestibility of feed nutrients. One of these dietary additives is Betulin extracted from birch bark, which has antioxidant and immunomodulatory properties. In this regard, the purpose of the research was to study the influence of compound feed with the introduction of 2 % feed additive based on a synthetic microspherical nanoporous carbon carrier containing biologically active Betulin in its composition on the productivity of broilers. The results of studies on broiler chickens using compound feed with 2 % feed additive have been presented in the article. It has been found that the feed consumption per 1 kg of live weight gain in poultry of the experimental group have been lower by 9,5 % due to better absorption of nutrients, the live weight of broiler chickens of the experimental group at the age of 42 days was 2625,6 g and it was higher than the control group by 3,68 %. The digestibility of crude protein and fat reached 92,73 and 88,03 %, which exceeded the control group by 1,03 and 3,03 abs.%, respectively. It has been found that the use of compound feed with 2 % of the feed additive with the biologically active substance is of practical importance in connection with the increase in the productivity of broilers and the level of profitability of meat production by 8,2 abs.%.

The physiological state of animals is to a certain extent characterized by hematological indicators, since blood occupies a special place in the body. By transferring nutrients and biologically active substances, the blood performs the general regulation of vital functions of the body. The use of Vitartil as a feed additive in the diets of animals leads to an increase in glycolytic processes and a decrease in the body’s need for oxygen delivery, which contributes to the growth of the animal body. The purpose of the research was to study the changes in blood parameters taking into account the age of Hereford steers against the background of the use of Vitartil feed additive to the main diet and its effect on the productive traits of animals. In order to carry out the experiment two groups of steers-analogs of Hereford breed have been formed at the age of 6 months per 10 heads in each. The difference between the groups was that the steers of the experimental group have been received the feed additive Vitartil in addition to the main diet. The greater yield of the meat part was in the steers of the experimental group in comparison with the herdmates of the control group. This indicator in the experimental group was 78,9 %, while in the control group it was lower by 1,6 %. The animals of the experimental group were superior to their herdmates in terms of meat weight by 1,4 kg or by 8,8 %. The relative number of bones was lower in the experimental group and amounted to 18,4 %, and in the control group 19,2 %. The meat content index was high in the steer have been received the biologically active additive Vitartil and was 4,3, and in the control group 4,0. It has been found that the use of Vitartil feed additive to the main diet of Hereford steers increases the blood content of hemoglobin and glucose, which has a certain effect on the morphological and chemical composition of meat, as well as increases its energy value.

Context Montmorillonite (MMT), as a potential antibiotic alternative, has rarely been reported in broiler chickens. Aims The present study was conducted to determine the effects of dietary MMT supplementation on the growth performance, immunity, intestinal microflora and morphological features of broilers. Methods One-day-old male broilers (Arbor Acres; n = 240) were randomly assigned to the following four groups before a 42-day feeding trial: (i) basal diet with no supplementation (control group); (ii) basal diet supplemented with 0.1% MMT; (iii) basal diet supplemented with 0.2% MMT; and (iv) basal diet supplemented with 0.3% MMT. Key results The results demonstrated that supplementation with 0.1% and 0.2% MMT markedly improved the average daily gain of broilers compared with the control group. Moreover, supplementation with 0.1% MMT remarkably decreased the total viable count of Escherichia coli in the caecum of broilers. Compared with the control group, 0.1% MMT supplementation significantly elevated the concentrations of secretory immunoglobulin A and systemic immunoglobulin G in the jejunal mucosa of broilers (P < 0.05). Furthermore, MMT supplementation was associated with a greater villus height (VH) and a higher ratio of VH to crypt depth (CD) in the intestinal mucosa of broilers (P < 0.05). However, in the duodenal and jejunal mucosa, dietary supplementation with MMT exhibited no significant (P > 0.05) impact on the CD values of broilers compared with the control group. Conclusions This study showed that supplementation with 0.1% MMT could enhance broiler growth, increase the small intestinal VH and the VH:CD ratio, promote the intestinal-mucosa development, affect microflora population and improve immune function in the intestinal tract of broilers. Implications Supplementation with MMT could alter morphological changes in the intestinal villi, regulate microbial population and improve immune function in the intestinal tract, thus contributing to broiler growth. Our results indicated that MMT may serve as a natural beneficial feed additive for poultry gut health.