Search

The neural mechanisms of torpor have essential reference significance for medical methods and long-term manned space. Changes in electrophysiology of suprachiasmatic nucleus (SCN) conduce to revealing the neural mechanisms from the torpor to arousal. Due to the lower physiology state during the torpor, it is a challenge to detect neural activities in vivo on freely behaving mice. Here, we introduced a multichannel microelectrode array (MEA) for real-time detection of local field potential (LFP) and action potential (spike) in the SCN in induced torpor mice. Meanwhile, core body temperature and behaviors of mice were recorded for further analysis. Platinum nanoparticles (PtNPs) and Nafion membrane modified MEA has a lower impedance (16.58 ± 3.93 kΩ) and higher signal-to-noise ratio (S/N = 6.1). We found that from torpor to arousal, the proportion of theta frequency bands of LFPs increased, spike firing rates rapidly increased. These results could all be characteristic information of arousal, supported by the microscopic neural activity promoting arousal in mice. MEA displayed real-time dynamic changes of neuronal activities in the SCN, which was more helpful to analyze and understand neural mechanisms of torpor and arousal. Our study provided a factual basis for the neural state in SCN of induced non-hibernating animals, which was helpful for the application of clinics and spaceflight.

The medial amygdala (MA) plays an important role in the innate fear circuit. However, the electrophysiological mechanism of MA for processing innate fear needs to be further explored. In this study, we fabricated microelectrode arrays (MEAs) with detecting sites arranged to match the location and shape of MA in mice and detected the electrophysiology in freely behaving mice under 2-methyl-2-thiazoline (2MT)-induced fear. The detection performance of MEA is improved by modifying metal nanoparticles and conductive polymers (PtNPs/PEDOT:PSS). After modification, the impedance magnitude and phase of electrodes were decreased to 27.0 ± 2.3 kΩ and −12.30 ± 0.52°, respectively, leading to a signal-to-noise ratio of 10. Its electrochemical stability and mechanical stability were also verified by cyclic voltammetry (CV) sweeping and ultrasonic vibration. MEAs were then implanted into the MA of mice, and the electrophysiology and behavioral characteristics were synchronously recorded and analyzed. The results showed that 2MT induced strong defensive behaviors in mice, accompanied by increases in the average spike firing rate and local field potential (LFP) power of MA neurons. According to principles commonly applied to cortical extracellular recordings, the recorded neurons are divided into two classes based on waveforms. Statistics showed that about 37% of type 1 neurons (putative GABAergic neurons) and 87% of type 2 neurons (putative glutamatergic neurons) were significantly activated under innate fear. At the same time, the firing rate of some activated neurons had a good linear correlation with the freezing rate.

The present work was addressed to low temperature oxidation and its kinetics of biomass chars. A cornstalk was pyrolyzed at 500 °C and 250 °C. The resulting chars and the cornstalk were subject to thermo-gravimetric analysis and differential scanning calorimetry (TGA–DSC) and isothermal calorimetry. The results demonstrated that combining the two thermal analyses enabled describing the low temperature oxidation behavior of the chars from close to room temperature to ignition. The derived activation energies from both techniques were well within the range of TGA and DSC measured values for various carbonaceous materials in the literature. Comparisons indicated that 500 °C char exhibited higher heat generation reactivity than 250 °C torrefied char and the cornstalk. Both TGA–DSC and isothermal calorimetry showed that the 250 °C char was slightly less reactive than the cornstalk, implying that torrefaction at 250 °C is potential to reduce the proneness of the cornstalk to self-heating.