1. A useful spectrophotometric rate assay for pepsin
- Author
-
Stein Tp, Reid Tw, and Fahrney D
- Subjects
Swine ,Ultraviolet Rays ,Sulfur Acids ,Biophysics ,Glycine ,Peptide ,Biochemistry ,Hydrolysis ,chemistry.chemical_compound ,fluids and secretions ,Sulfite ,Pepsin ,Phenols ,Spectrophotometry ,medicine ,Animals ,Molecular Biology ,chemistry.chemical_classification ,Chromatography ,medicine.diagnostic_test ,biology ,Chemistry ,Esters ,Cell Biology ,Pepsin A ,Kinetics ,biology.protein ,Substrate specificity - Abstract
Phenyl sulfite is a specific substrate for pepsin, whose hydrolysis can be followed spectrophotometrically. This compound is superior to peptide substrates in its ease of synthesis and its ability to be monitered spectrophotometrically. The sulfite assay is therefore the method of choice for both routine and detailed assays of pepsin.
- Published
- 1971