Search

Morphotypes of arbuscular mycorrhizal (AM) symbiosis, Arum, Paris, and Intermediate types, are mainly determined by host plant lineages. It was reported that the phytohormone gibberellin (GA) inhibits the establishment of Arum-type AM symbiosis in legume plants. In contrast, we previously reported that GA promotes the establishment of Paris-type AM symbiosis in Eustoma grandiflorum, while suppressing Arum-type AM symbiosis in a legume model plant, Lotus japonicus. This raises a hitherto unexplored possibility that GA-mediated transcriptional reprogramming during AM symbiosis is different among plant lineages as the AM morphotypes are distinct. Here, our comparative transcriptomics revealed that several symbiosis-related genes were commonly upregulated upon AM fungal colonization in L. japonicus (Arum-type), Daucus carota (Intermediate-type), and E. grandiflorum (Paris-type). Despite of the similarities, the fungal colonization levels and the expression of symbiosis-related genes were suppressed in L. japonicus and D. carota but were promoted in E. grandiflorum in the presence of GA. Moreover, exogenous GA inhibited the expression of genes involved in biosynthetic process of the pre-symbiotic signal component, strigolactone, which resulted in the reduction of its endogenous accumulation in L. japonicus and E. grandiflorum. Additionally, differential regulation of genes involved in sugar metabolism suggested that disaccharides metabolized in AM roots would be different between L. japonicus and D. carota/E. grandiflorum. Therefore, this study uncovered the conserved transcriptional responses during mycorrhization regardless of the distinct AM morphotype. Meanwhile, we also found diverse responses to GA among phylogenetically distant AM host plants.

Eustoma grandiflorum (Raf.) Shinners is a popular cut flower due to its beautiful morphological characteristics and extended vase life. Here, the complete plastome sequence of E. grandiflorum was reported based on the Illumina HiSeq Platform. The plastome sequence is 154,230 bp in length with a typical quadripartite structure, containing a pair of inverted repeated (IR) regions (25,755 bp) that are separated by a large single copy (LSC) region of 84,297 bp, and a small single copy (SSC) region of 18,423 bp. A total of 130 functional genes were annotated, including 84 protein-coding genes, 38 tRNA genes, and 8 rRNA genes. The complete plastome sequence of E. grandiflorum will provide a valuable resource for its garden utilization and the phylogenetic studies of Gentianaceae.

In the aerial plant organs, cuticular wax forms a hydrophobic layer that can protect cells from dehydration, repel pathogen attacks, and prevent organ fusion during development. The MIXTA gene encodes an MYB-like transcription factor, which is associated with epicuticular wax biosynthesis to increase the wax load on the surface of leaves. In this study, the AmMIXTA-homologous gene EgMIXTA1 was functionally characterized in the Eustoma grandiflorum. EgMIXTA1 was ubiquitously, but highly, expressed in leaves and buds. We identified the Eustoma MIXTA homolog and developed the plants for overexpression. EgMIXTA1-overexpressing plants had more wax crystal deposition on the leaf surface compared to wild-type and considerably more overall cuticular wax. In the leaves of the overexpression line, the cuticular transpiration occurred more slowly than in those of non-transgenic plants. Analysis of gene expression indicated that several genes, such as EgCER3, EgCER6, EgCER10, EgKCS1, EgKCR1, and EgCYP77A6, which are known to be involved in wax biosynthesis, were induced by EgMIXTA1-overexpression lines. Expression of another gene, WAX INDUCER1/SHINE1, encoding a transcription factor that stimulates the production of cutin, was also significantly higher in the overexpressors than in wild-type. However, the expression of a lipid-related gene, EgABCG12, did not change relative to the wild-type. These results suggest that EgMIXTA1 is involved in the biosynthesis of cuticular waxes.

In the aerial plant organs, cuticular wax forms a hydrophobic layer that can protect cells from dehydration, repel pathogen attacks, and prevent organ fusion during development. The MIXTA gene encodes an MYB-like transcription factor, which is associated with epicuticular wax biosynthesis to increase the wax load on the surface of leaves. In this study, the AmMIXTA-homologous gene EgMIXTA1 was functionally characterized in the Eustoma grandiflorum. EgMIXTA1 was ubiquitously, but highly, expressed in leaves and buds. We identified the Eustoma MIXTA homolog and developed the plants for overexpression. EgMIXTA1-overexpressing plants had more wax crystal deposition on the leaf surface compared to wild-type and considerably more overall cuticular wax. In the leaves of the overexpression line, the cuticular transpiration occurred more slowly than in those of non-transgenic plants. Analysis of gene expression indicated that several genes, such as EgCER3, EgCER6, EgCER10, EgKCS1, EgKCR1, and EgCYP77A6, which are known to be involved in wax biosynthesis, were induced by EgMIXTA1-overexpression lines. Expression of another gene, WAX INDUCER1/SHINE1, encoding a transcription factor that stimulates the production of cutin, was also significantly higher in the overexpressors than in wild-type. However, the expression of a lipid-related gene, EgABCG12, did not change relative to the wild-type. These results suggest that EgMIXTA1 is involved in the biosynthesis of cuticular waxes.

Eustoma grandiflorum, commonly known as prairie gentian or Texas bluebells, is among the most popular agriculturally propagated species of cut flowers. Due to its widespread appeal, there is increasing interest in understanding the molecular genetic factors underlying floral development and resistance to abiotic stresses. We analyzed 18 potential reference genes in different organs, at different floral developmental stages and under drought- and salt-stress treatments, for use in RT-qPCR analysis. A total of four analytical tool packages, including geNorm, NormFinder, BestKeeper, and RefFinder were employed to determine the most appropriate reference genes under each treatment condition. The results demonstrate that different reference genes should be used for normalization under different experimental treatments. EgPP and EgPP2A2 were the most stable internal control genes across different organ types, EgPP and Eg18S were the most stable under salt-stress, EgPP and EgACT1 were the most stable across different floral development stages, and EgEF1A and EgTUA were the most stable reference genes under drought-stress. Additional gene expression analyses of EgMIXTA1, EgTOE1, and EgP5CS1 further confirmed the applicability of these reference genes. The results represent a significant contribution to future studies of reference gene selection for the normalization of gene expression in Eustoma grandiflorum.

Made available in DSpace on 2018-12-11T17:37:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-01-01. Added 1 bitstream(s) on 2019-10-09T18:33:17Z : No. of bitstreams: 1 S2447-536X2018000100012.pdf: 1864965 bytes, checksum: 58843057c88e29491e1f4a17cc156908 (MD5) Floriculture industry demands for products with high quality and durability; however, there is a lack of studies related to the postharvest physiology of cut flowers. We aimed to study phenolic contents of lisianthus (Eustoma grandiflorum) stems treated with ethylene inhibitors (1-Methylcyclopropene - 1-MCP and Salicylic Acid - SA) and different storage temperatures (room at 24 ± 2 °C and pre-exposure to the cold chamber at 9 ± 2 °C for 24 hours) during the post-harvest. Total soluble carbohydrate contents decreased during the experimentation, characterizing the consumption of the reserves during lisianthus post-harvest. The 1-MCP treatment slowed the decrease of total soluble carbohydrate contents. SA treatment had the lowest total soluble carbohydrate contents in both storage temperatures. Universidade Estadual Paulista (UNESP) Instituto de Botânica Universidade Estadual Paulista (UNESP)

Background Eustoma grandiflorum is a new floral crop for the international flowers market, moderately cold-tolerant annual or biennial plant. A large number of seedlings can be produced by seed propagation but the quality is not uniform due to variations in the flowering time, plant height, and the number of flowers. Propagation of Eustoma grandiflorum plant by tissue culture technique is relatively low. Inducing mutations is one of the powerful tools for breeding biotechnology. Laser mutagenesis is an easy and new tool. The goal of the present work was to investigate the influence of laser irradiation on in vitro growth, anatomy, flowering, chemicals composition, and gene mutagenesis. Results The most of morphological, floral parameters, total chlorophyll, carotenoids, and anthocyanin pigment contents in the flower recorded increment by most treatments of laser types. The highest survival percentage of acclimatized plants (95%) and highest values of number of branches and branches length (cm) were obtained from treated plantlets by 20 min of green laser, while most of highest floral parameters, anthocyanin pigment contents in flower, and anatomical structural parameters recorded increasing using 20 min of blue laser and 20, 25 min of green and red laser, respectively. Contrary, the lowest values of photosynthetic pigments and carotenoids were obtained from 20 min of green laser. Conclusions The current research concluded that laser irradiation has remarkable effect on plant morphology, flowering, chemical constituents, and gene mutagenesis.

Made available in DSpace on 2022-04-28T19:00:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-01-10 This study evaluated the activity of the enzyme polyphenol oxidase (PPO) and the content of soluble protein present in lisianthus bud flowers, flowers and leaves in room temperature (24±2°C) and pre-exposure cold chamber at 9±2°C for 24 h, in order to examine a possible correlation between these parameters and postharvest longevity of lisianthus flowers. After treatments, flowers were kept in pots with water, stored at room temperature and evaluated every three days until the end of their decorative life for biochemical analyzes. During the experimental period the enzymatic activity increased with the aging of the material, directly related to the high concentration of phenolics that were accumulated in injured tissue, providing browning, while soluble protein content slightly decreased. Thus, PPO enzyme activity can be applied for plant senescence evaluation, acting as a biochemical marker for product visual quality. Universidade Estadual Paulista Faculdade de Ciências Agronômicas Universidade Estadual Paulista Instituto de Biociências Universidade Estadual Paulista Faculdade de Ciências Agronômicas Universidade Estadual Paulista Instituto de Biociências

Lisianthus [Eustoma grandiflorum (Raf.) Shinn.], a member of the family Gentianaceae, is native to the central and southern part of United States. Lisianthus is a relatively new floral crop to the international market, quickly rankes in the top ten cut flowers worldwide due to its garish flowers and excellent post-harvest life. Due to its importance there are several tissue culture studies working on the micropropagation of this plant by using a variety of plant growth regulators (PGRs). With the aim of contribution to these works in the present study we aimed to investigate the effect of Gibberellic acid (GA3) at different concentrations (0.0, 0.5, 1.0, 2.0 and 4.0 mg l-1) on morphometric parameters of Lisianthus during 30 and 80-day of incubation periods. In sum, our findings revealed that according to the results, there was no significant change for shoot number, shoot length and root number but very slight increase in number of leaves (1.0 mg l-1) and root length (0.5 mg l-1) of GA3 during 30-day incubation period. On the other hand, for 80-day incubation period, the highest root length and number of leaves were obtained (respectively, 2.48 ± 1.25 cm and 14.17 ± 4.59) for 2.0 mg l-1 concentration. In addition, shoot length to be found at the highest rate for 1.0 mg l-1 (3.32 ± 0.34 cm). 80-day incubation period was found to be more effective than in comparison to 30-day incubation period in terms of number of leaves, shoot and root length., Lisianthus [Eustoma grandiflorum (Raf.) Shinn.],Gentianaceae familyasının bir üyesi olup Orta ve Güney Amerika’nın yerelbitkisidir. Hasat sonrası kaliteli ömrü olması ve gösterişli çiçeklere sahipolmasından dolayı, dünya çapındaki uluslararası pazarda oldukça yeni birçiçekli ürün olarak, kesme çiçek sektöründe hızlı bir şekilde ilk onsıralamasına girmiştir. Sahip olduğu öneminden dolayı bu bitkininmikroçoğaltımı üzerinde çeşitli bitki büyüme düzenleyicileri (BBDler)kullanılarak yapılan birçok doku kültürü çalışması mevcuttur. Yapılan buçalışmalara katkı sağlamak amacı ile 30 ve 80 günlük inkübasyon periyodusüresince farklı konsantrasyonlardaki (0.0, 0.5, 1.0, 2.0 and 4.0 mg l-1)Giberellik asit (GA3)’in Lisianthus’un morfometrik parametreleriüzerindeki etkisini araştırmak amaçlanmıştır. Özetle, verilen sonuçlara görebulgularımız, 30 günlük inkübasyon periyodu süresince GA3’in sürgünsayısı, sürgün uzunluğu ve kök sayısı üzerinde önemli bir değişikliğe nedenolmadığını fakat yaprak sayısı (1.0 mg l-1) ve kök uzunluğu (0.5 mgl-1) parametreleri üzerinde nispeten artış gösterdiğini açıklığakavuşturmuştur. Diğer bir taraftan 80 günlük inkübasyon periyodu süresince, enyüksek sürgün uzunluğu ve yaprak sayısı sırasıyla 2.48 ± 1.25 cm ve 14.17 ±4.59 olarak 2.0 mg l-1 konsantrasyondan elde edilmiştir. Buna ekolarak, en yüksek sürgün uzunluğu 1.0 mg l-1 konsantrasyonda (3.32 ±0.34 cm) bulunmuştur. Yaprak sayısı, sürgün uzunluğu ve kök uzunluğu açısından30 günlük inkübasyon periyodu ile karşılaştırıldığında 80 günlük inkübasyon periyodudaha etkili bulunmuştur.

COMPARAÇÃO DE DOIS MÉTODOS DE APLICAÇÃO DE FUNGICIDAS,IRRIGAÇÃO POR GOTEJAMENTO E PULVERIZAÇÃO CONVENCIONAL NO CONTROLE DO MOFO CINZENTO (Botrytis cinerea Pers.: Fr.) EM VASOS COM PLANTAS DE LISIANTHUS (Eustoma grandiflorum (Raf.) Shinn.) Ieoschua Katz1; Antonio Ribeiro da Cunha2; Antônio de Pádua Sousa1; Egberto Egon de Herdani11Departamento de Engenharia Rural, Faculdade de Ciëncias Agronömicas, Universidade Estadual Paulista, Botucatu, SP, iekatz@fca.unesp.br2 Departamento de Recursos Naturais, , Faculdade de Ciëncias Agronömicas, Universidade Estadual Paulista, Botucatu, SP 1 RESUMO Novas técnicas de combate às doenças têm sido usadas, como os defensivos hidrossolúveis via água de irrigação, a quimigação, um procedimento recente que reduz a mão de obra, com uniformidade na aplicação, menor contato do operador com produtos tóxicos, e menores impacto ambiental e custo de produção. Visando o controle do mofo cinzento (Botrytis cinerea) na cultura do Lisianthus, este trabalho teve o intuito de avaliar dois métodos de aplicação de fungicidas, a aplicação via gotejamento (fungigação) e a pulverização, com diferentes tratamentos. Os fungicidas utilizados foram: thiofanato metílico (50 g i.a. L-1), thiofanato metílico + chlorothalonil (50 g i.a. L-1 + 35 g i.a. L-1) e iprodione (50 g i.a. L-1). Avaliou-se o número de lesões, o número de botões florais e a altura das plantas de Lisianthus. Foi possível concluir que, tanto na fase de crescimento (número de lesões na planta) como na fase final (número de botões florais) da cultura de Lisianthus, os tratamentos 2 (thiofanato metílico + chlorotalonil) e 3 (iprodione) foram os mais eficientes. Considerando que o tratamento 2 é uma mistura de dois fungicidas, o primeiro sistêmico e o segundo de contato, independente das vias de aplicação, a mistura aumentou a eficiência em relação ao tratamento 1 (thiofanato metílico). Com isso, a aplicação por gotejamento (fungigação), revelou eficiência equivalente à técnica de pulverização. UNITERMOS: Lisianthus, quimigação, irrigação por gotejamento, controle de doenças . KATZ , I.; CUNHA, A. R. da; SOUSA, A. de P.; HERDANI, E.E.de. COMPARISON OF TWO METHODS OF FUNGICIDE APPLICATION, DRIP IRRIGATION AND CONVENTIONAL SPRAYING CONTROL FOR CINEREOUS MILDEW (Cinerea botrytis Pers.:Fr.) IN POTTED PLANTS OF LISIANTHUS (Eustoma grandiflorum (Raf.) Shinn.) 2 ABSTRACTPlant disease control techniques are used through the irrigation water, which reduces the labor and it improves application uniformity with smaller contact of the operator with toxic products, lower environmental impact and lower production cost. In order to control Botrytis cinerea the Lisianthus culture, this work aimed to evaluate two fungicide application methods with different treatments. The fungicides were: thiophanate methyl (50 gi.a. L-1), thiophanate methyl + chlorothalonil (50 g i.a. L-1 + 35 g i.a. L-1) and iprodione (50 g i.a. L-1). Number of lesions, number of diseased floral buttons and height of the Lisianthus plants were evaluated. It was possible to deduce, that in the growth stage (number of lesions in the plant) as well as in the final stage (number of floral buttons) of the Lisianthus culture, the most efficient treatments were 2 (thiophanate methyl + chlorothalonil) and 3 (iprodione). Considering that treatment 2 is a mixture of two fungicides, a systemic and a contact one, independently of application methods, the mixture increased efficiency in relation to treatment 1 (thiophanate methyl). Thus, chemigation was as efficient as spreading technique. KEYWORDS: Lisianthus, chemigation, drip irrigation, disease control

In March 1999, we found prairie gentian (Eustoma grandiflorum) infected with powdery mildew in a greenhouse in Oita Prefecture, Japan. Morphological observation revealed that the causal fungus belongs to the mitosporic genus Oidium subgenus Pseudoidium [teleomorph: Erysiphe sensu Braun and Takamatsu (2000)]. Precise taxonomic position of the fungus, however, is uncertain due to lack of the perfect stage. We determined the nucleotide sequence of the rDNA ITS region of the fungus. Comparison of the sequence with those obtained from DNA databases of this fungal group revealed that the sequence is identical to those of powdery mildews from garden four-o'clock (Mirabilis jalapa) and broad bean (Vicia faba). Inoculation of an isolate from garden four-o'clock caused mildew on prairie gentian and broad bean, suggesting that the prairie gentian mildew originates from garden four-o'clock or broad bean. Molecular phylogenetic analysis indicated a close relationship of this fungus to Erysiphe baeumleri on Vicia spp. and E. trifolii on Trifolium pratense. From these results, we propose that prairie gentian mildew diverged from a Fabaceae-parasitic ancestor.

In a controlled environment, light from light-emitting diodes (LEDs) has been associated with affecting the leaf characteristics of Eustoma. LEDs help plant growth and development, yet little is known about photosynthetic performance and related anatomical features in the early growth stage of Eustoma leaves. In this study, we examined the effects of blue (B), red (R), and white (W) LEDs on the photosynthetic performance of Eustoma leaves, as well as leaf morphology and anatomy including epidermal layer thickness, palisade cells, and stomatal characteristics. Leaves grown under B LEDs were thicker and had a higher chlorophyll content than those grown under the R and W LEDs. Leaves under B LEDs had greater net photosynthetic rates (A), stomatal conductance (gs), and transpiration rates (E), especially at a higher photon flux density (PPFD), that resulted in a decrease in the intercellular CO2 concentration (Ci), than leaves under the W and R LEDs. B LEDs resulted in greater abaxial epidermal layer thickness and palisade cell length and width than the R and W LED treatments. The palisade cells also developed a more cylindrical shape in response to the B LEDs. B LED leaves also showed greater guard cell length, breadth, and area, and stomatal density, than W or R LEDs, which may contribute to increased A, gs and E at higher PPFDs.

Se evaluó la vida en florero de inflorescencias de lisianthus ABC Blue Rim almacenadas en húmedo o seco durante 5, 10 y 15 días a 3 °C y 85% de humedad relativa (HR) en oscuridad; antes del almacenamiento se aplicó una solución pulso 3% de sacarosa + 200 mg L-1 de Hidroxiquinoleína citrato por 24 h. Al salir de temperatura baja, la vida en florero se evaluó en una cámara con una temperatura de 20 ± 1 °C y 80 ± 2% HR, periodo de 12 h luz/oscuridad y una PAR de 173 ± 50 μmol m2 s-1. Un grupo de inflorescencias no se almacenaron a temperatura baja (testigo); estas inflorescencias mostraron un comportamiento climatérico, un incremento de peso fresco relativo (7.2%), consumo de agua (31.1 mL tallo-1) y conductancia estomática (170 mmol m2 s-1), a los nueve días después de cosecha se tuvieron tres flores abiertas con regular apariencia y la actividad en catalasa se incrementó hasta 6.3 U g-1 de peso fresco, en tanto que la actividad de peroxidasa se mantuvo constante durante la vida en florero (entre 0.1 y 0.4 U g-1 de peso). Las flores almacenadas en agua mostraron comportamiento climatérico similar al testigo, menor incremento de peso fresco relativo, similar consumo de agua y mayor conductancia estomática que las flores testigo; se incrementó la vida poscosecha hasta 19 días, la actividad enzimática de catalasa y peroxidasa se incrementaron en forma similar al testigo. El almacenamiento en seco solo fue factible por cinco días