1. Absorption Peaks: α, β, γ and Their Covariance with Age and Hemoglobin in Human Blood Samples Using Photoacoustic Spectroscopy
- Author
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Alfredo Cruz-Orea, F. A. Domínguez-Pacheco, E. Martinez-Ortiz, Claudia Hernández-Aguilar, J. L. González-Domínguez, and F. Sánchez-Sinencio
- Subjects
education.field_of_study ,Nuclear magnetic resonance ,Filter paper ,Chemistry ,Population ,Hemoglobin ,Absorption (chemistry) ,Covariance ,Condensed Matter Physics ,Spectroscopy ,Ligand (biochemistry) ,education ,Photoacoustic spectroscopy - Abstract
This study reports the absorption peaks α, β, γ in the Soret band of photoacoustic (PA) signals and their covariance with age and hemoglobin in human blood samples through PA spectroscopy. Samples were taken randomly from a masculine population grouped in three categories according to age: infants, young adults, and senior adults. Samples were prepared with two drops of blood from a 0.5 mL insulin syringe with a needle gauge 31G over 5 mm circles of filter paper. It was observed that the PA signal, the amplitude as a function of the wavelength, has a behavior as that reported for human blood for the three absorption peaks α, β, γ. In particular, the ratio γ/ β is due to electronic transitions associated with charge-transfer interactions of iron orbitals with the ligand states. Through an evaluation of optical absorption peaks in blood samples and their covariance with age and hemoglobin concentration, a relationship was found for the ratio peaks γ/ β and γ/ α with such parameters. Specifically, a negative covariance in the Soret band of the ratio peaks γ/ β and γ/ α with respect to both age and hemoglobin was found. This showed a tendency in their behavior. Further experiments of different populations may corroborate these conclusions.
- Published
- 2012