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This study aimed to assess the influence of dietary supplementation of ε-polylysine on the gut microbiota and host nutrient metabolism, which is not systematically discussed by multi-omics analysis. A total of 40 mice were randomly divided into two groups exposed to either a basal diet (AIN-76A) or a basal diet with 150 ppm ε-polylysine. Fecal samples were collected for gut bacteria identification. Liver and plasma samples were collected for metabolomic and proteomic analyses. The results showed that ε-polylysine decreased the body weight of mice and affected the presence of certain types of intestinal microorganisms. The richness of the microbiota and number of phyla increased with age. ε-Polylysine affected the presence of genera and species, and either regulated or took part in the metabolism of energy, nitrogen, amino acids, lipids, carbohydrates, glycans, cofactors, and vitamins. The metabolite profiling showed that lipid and lipid-like molecules metabolites occupied the majority percent of plasma and liver metabolites. Additionally, ε-polylysine regulated the key role of metabolites and related metabolic enzymes in the metabolic pathways, especially phospholipid metabolism. In conclusion, dietary ε-polylysine improved the immunity of growing mice, and had a greater effect on the anabolism of nutrients in adult mice.

Brittle Culm 15 (BC15) gene encodes a membrane-associated chitinase-like protein that participates in cellulose synthesis, and BC15 gene mutation affects cell wall composition in plant, such as cellulose or hemicellulose. The present study was designed to investigate the changes of carbohydrates composition in bc15 mutant straw, and the resulting consequence on rumen fermentation, methanogenesis, and microbial populations (qPCR) during in vitro ruminal fermentation process. Two substrates, bc15 mutant and wild-type (WT) rice straws, were selected for in vitro rumen batch culture. The first experiment was designed to investigate the kinetics of total gas and CH4 production through 48-h in vitro ruminal fermentation, while the second experiment selected incubation time of 12 and 48 h to represent the early and late stage of in vitro ruminal incubation, respectively, and then investigated changes in biodegradation, fermentation end products, and selected representative microbial populations. The bc15 mutant straw had lower contents of cellulose, neutral detergent fiber (NDF) and acid detergent fiber (ADF), and higher contents of water-soluble carbohydrates, neutral detergent solubles (NDS) and monosaccharides. The bc15 mutant straw exhibited a distinct kinetics of 48-h total gas and CH4 production with faster increases in early incubation when compared with WT straw. The bc15 mutant straw had higher DM degradation, NDF degradation and total volatile fatty acid concentration at 12 h of incubation, and lower NDF degradation and CH4 production at 48 h of incubation, together with lower acetate to propionate ratio and ADF degradation and higher butyrate molar percentage and NDS degradation at both incubation times. Furthermore, the bc15 mutant straw resulted in greater 16S gene copies of F. succinogenes, with lower 18S gene copies of fungi at both incubation times. These results indicated that the BC15 gene mutation decreased fibrosis of cell wall of rice straw, enhanced degradation at the early stage of rumen fermentation, and shifts fermentation pattern from acetate to propionate and butyrate production, leading to the decreased volume and fractional rate of CH4 production. However, BC15 gene mutation may enhance hardenability of cell wall structure of rice straw, which is more resistant for microbial colonization with decreased fiber degradation. Thus, this study modified rice straw by manipulating a cell wall biosynthesis gene and provides a potential strategy to alter degradation and CH4 production during in vitro ruminal fermentation process.

This experiment was conducted to examine the effects of substituting mixed silage (fresh ramie: dry rice straw = 80: 20; kg: kg) composed of fresh forage ramie, also known as ‘China grass’, Boehmeria nivea L. Gaud (a nettle native to Asia) and rice straw for corn silage and alfalfa hay on the production performance, milk components, rumen fermentation parameters, and nutrient digestion in dairy cows. Thirty multiparous Chinese Holstein cows (629 ± 59.2 kg of BW, 25 ± 4.7 kg of milk yield, and 100 ± 18 DIM; mean ± SD) were randomly divided into three groups. The experimental treatments containing three diets, each consisting of differing proportions of mixed silage (0%, 10%, and 20%, designated as CON, MS1, and MS2, respectively) as a substitution for corn silage and alfalfa hay. There were no effects of mixed silage diets on dry matter intake (DMI), 3.5% fat-corrected milk (FCM) yield, milk protein percentage, milk somatic cell count, and milk urea nitrogen (MUN), but linearly increased total solids (p = .03) and milk fat percentage (p = .001) in cows fed the MS1 and MS2 diets. Feeding mixed silage diets linearly reduced milk yield (p = .01) and milk lactose percentage (p = .01), and had linearly increased rumen pH values (p = .01). There were no observable differences in other rumen fermentation parameters between the mixed silage and control diets. Mixed silage diets exhibited increased DM digestibility (linearly, p = .04; quadratically, p = .017) and linearly decreased crude protein digestibility (p = .05), but we observed no differences in the apparent total-tract digestibility of EE, NDF, and ADF between the control and mixed silage diets. In conclusion, using ramie and rice straw mixed silage proved beneficial by increasing milk fat percentage, milk solids, and DM digestibility, ramie could be used as a potential forage resource in dairy cow diets.Highlights We tested the effects of substituting alfalfa hay and corn silage with mixed silage composed of fresh forage ramie and rice straw on dairy cows Mixed silage diets had no effect on dry matter intake (DMI) Mixed silage diets reduced milk yield and milk lactose percentage

Given the antibacterial effects of ε-polylysine acting on cell membranes, and that glycerol phospholipids are important components of the cell membrane, we hypothesized that ε-polylysine may regulate glycerophospholipid metabolism by modifying the gut microbiota. To test this hypothesis, we treated post-weaning C57 mice with different levels of ε-polylysine (0, 300, 600, and 1,200 ppm) in their basic diet. The growth performance and morphology of intestine were then determined. Modification of the gut microbiota and their function were analyzed using 16S rDNA sequencing. Metabolite identification was performed using the LC-MS method. The results showed that body weight decreased with an increasing supplemental level of ε-polylysine from 5 to 7 weeks (P < 0.05), but no significant difference was observed after 8 weeks (P > 0.05). Supplementation with 1,200 ppm ε-polylysine changed the morphology of the jejunum and ileum, increased the villus length, decreased the crypt depth of the jejunum, and decreased the villus length and crypt depth of the ileum (P < 0.05). ε-Polylysine shifted the intestine microbiota by changing alpha diversity (Chao 1, observed species, Shannon, and Simpson indices) and varied at different times. ε-polylysine decreased Firmicutes and increased Bacteroidetes at 4 week, but increased Firmicutes and decreased Bacteroidetes at 10 week. ε-Polylysine regulated genera associated with lipid metabolism such as Parabacteroides, Odoribacter, Akkermansia, Alistipes, Lachnospiraceae UCG-001, Collinsella, Ruminococcaceae, and Intestinimonas. During the adult period, the genera Alistipes, Lachnospiraceae UCG-001, and Streptomyces were positively associated with PC, PE, LysoPC, LysoPE, 1-Arachidonoylglycerophosphoinositol and OHOHA-PS (R > 0.6, P < 0.001), but changes in Blautia, Christensenellaceae R-7 group, Odoribacter, Allobaculum, Ruminococcaceae UCG-004, Ruminococcaceae UCG-005, and Lachnospiraceae UCG-010 were negatively correlated with glycerophospholipid metabolites (R < −0.6, P < 0.001). The abundance of glycerophospholipid metabolites, including PC, PE, lysoPC, and lysoPE, were decreased by ε-polylysine. Furthermore, ε-polylysine reduced the incidence of the genera including Ruminococcus, Prevotella, Prevotellaceae, Butyricimonas, and Escherichia-Shigella and reduced the abundance of Faecalibaculum, Christensenellaceae R-7 group, Coriobacteriaceae UCG-002. In conclusion, ε-polylysine modified gut microbiota composition and function while also restraining pathogenic bacteria. The glycerophospholipid metabolism pathway and associated metabolites may be regulated by intestinal bacteria.

The objectives of this study were to evaluate the contribution of urea on the nutritional quality and microbial community of ensiled alfalfa (Medicago sativa L.). Alfalfa silage was control group without urea (AL), supplementation with 0.5 % urea (AU1), or supplementation with 1 % urea (AU2). The silage tanks were opened and sampled after silage at 0, 15, 30, and 60 d. Results showed that AU2 had higher pH, ratio of NH3-N/TN and CP content than those in AL and AU1 while AU1 had higher ADF than that in AL and AU2 after 15 d silage. Richness and diversity indices of microbial communities in silage were no significant differences among AL, AU1 and AU2 group. Proteobacteria (58.23%) and Firmicutes (40.95%) were the predominant phylum in three groups during the silage process. The percent of community abundances on genera level of Enterobacteriaceae (37.61%) and Klebsiella (41.78%) in AL were a little higher than those in AU1 (30.39%, 25.02%) and AU2 (33.48%, 26.92%). These results showed that silage with urea alone could not improve the quality of alfalfa.

This study was conducted to determine the effects of oral administration with glutamate on metabolism of suckling piglets based on 1 H-Nuclear magnetic resonance (1 H NMR) spectroscopy through the level of metabolism. Forty-eight healthy [(Yorkshire × Landrace) × Duroc] piglets born on the same day with a similar birth bodyweight (1.55 ± 0.20 kg) were obtained from six sows (8 piglets per sow). The piglets from each sow were randomly assigned into four treatments (2 piglets per treatment). The piglets were given 0.09 g/kg body weight (BW) of sodium chloride (CN group), 0.03 g/kg BW monosodium glutamate (LMG group), 0.25 g/kg BW monosodium glutamate (MMG group) and 0.50 g/kg BW monosodium glutamate (HMG group) twice a day respectively. An 1 H NMR-based metabolomics' study found that the addition of monosodium glutamate (MSG) significantly reduced serum citrate content in 7-day-old piglets, while HMG significantly increased serum trimethylamine content and significantly reduced unsaturated fat content in 7-day-old piglets (p < .05). The content of glutamine, trimethylamine, albumin, choline and urea nitrogen was significantly increased and the creatinine content decreased significantly in the 21-day-old HMG (p < .05). Analysis of serum hormones revealed that glucagon-like peptide-1 (GLP-1) content in the 21-day-old HMG was highest (p < .05). The cholecystokinin (CCK) content in the HMG of 7-day-old piglets was lower than that in the LMG (p < .05), and the CCK content in the serum of the 21-day-old MMG was highest (p < .05). The serum leptin levels in the 21-day-old HMG were the lowest (p < .05). The serum insulin content in the 7-day-old MMG was highest (p < .05). This study suggests that MSG plays an important role in the metabolism of sugar, fat and protein (amino acids). These results provide a theoretical basis for designing piglet feed formulations.

Intestinal microbiota plays an important role in the health of animals. However, little is known about the gut microbiota in Ningxiang pigs. Thus, we investigated how dietary supplementation with different ε-polylysine concentrations (0, 20, 40, 80, and 160 ppm) affected the ileal microbiota in Ningxiang pigs using a replicated 5 × 5 Latin square method. Each experimental period included 10 days for diet adaptation, 3 days for feces collection and 2 days for digesta collection. The ileal contents were collected and used for sequencing of the V3–V4 hypervariable region of the 16S rRNA gene. The results revealed that ε-polylysine significantly decreased the digestibility of crude protein and crude fiber, as well as the utilization of metabolizable energy (P < 0.05). The relative abundances of 19 bacterial genera significantly increased, while those of 26 genera significantly decreased (P < 0.05). In addition, ε-polylysine increased the abundance of some bacteria (e.g., Faecalibacterium, Bifidobacterium, and lactic acid bacteria) and inhibited some other bacteria (e.g., Micrococcaceae, Acinetobacter, Anaerococcus, Peptoniphilus, Dehalobacterium, Finegoldia, Treponema, and Brevundimonas). Furthermore, based on the 16S rRNA gene data and data from the precalculated GreenGenes database, bacterial communities in the ileal contents exhibited enhanced functional maturation, including changes in the metabolism of carbohydrates, amino acids (e.g., alanine, lysine, tryptophan, cysteine, and methionine), cofactors, and vitamins (e.g., biotin, thiamine, and folate), as well as in the activity of the insulin signaling pathway. This study suggests that ε-polylysine may influence the utilization of feed nutrients by Ningxiang pigs, including proteins, lipids, metabolizable energy, and fiber, by regulating the gut microbiota.

Five white-rot fungi Pleurotus ostreatus, Lentinus edodes, Hericium erinaceus, Pleurotus eryngii and Flammulina filiformis were studied (solid-state incubation and in vitro gas production) to determine lignin degradation and optimal duration of fermentation of corn straw. All fungi significantly decreased lignin, with optimal reductions after 28 d. Although cellulose also decreased, L. edodes and P. eryngii minimized these losses. In intro dry matter digestibility, total volatile fatty acid concentration and total gas production of fermented corn straw decreased (P 0.001) as fermentation was prolonged, with improved rumen fermentability for all fungal treatments except F. filiformis. Total gas production in L. edodes did not decrease but peaked on day 28, whereas F. filiformis reduced methane emission. In conclusion, fermentation of corn straw with P. eryngii or L. edodes for 28 d degraded lignin and improved nutritional value as ruminant feed.

We hypothesized that ramie, Boehmeria nivea (a nettle native to Asia), can be used as a high-quality forage to replace alfalfa hay in diets of dairy cows. Accordingly, we aimed to examine the effects of substituting fresh forage ramie for alfalfa (Medicago sativa) hay on production performance, milk composition, and serum parameters of dairy cows. Thirty-two Holstein cows (body weight (BW) = 590 ± 50.6 kg) were randomly divided into four groups of eight cows. The experimental period lasted 10 weeks. The dietary treatments consisted of four proportions of fresh forage ramie (0, 33, 67, and 100%) as a substitute for alfalfa hay (designated as CON, FR33, FR67, and FR100, respectively). On days 69 and 70 of the experimental period, milk and blood samples were collected for analysis. We found no significant differences in the milk yield and milk quality (milk protein percentage, milk fat percentage, and milk lactose percentage) between the treatments. We did find that forage ramie significantly reduced dry matter intake (DMI) and 4% fat-corrected milk (FCM) yield. Meanwhile, no significant differences were detected in serum parameters between the treatments, with the exception of triglyceride (TG) and alanine aminotransferase (ALT). In conclusion, our data indicate that alfalfa hay can be replaced with forage ramie in the diet of Holstein cows in the milk lactation stage, with no negative effects on milk quality and blood parameters.

This study evaluated the effects of alkyl polyglycoside (APG), which is a non-ionic surfactant, on lactation performance, nutrient digestibility and blood metabolites in dairy cows. Twenty dairy cows were randomly divided into four groups and fed a basal diet that included pelleted concentrate, distillers grains, and fresh limpograss. The four treatments included 0, 5.5, 11 and 22 mL APG per kg of pelleted concentrate on a dry matter basis, treatments were defined as APG0, APG5.5, APG11, and APG22, respectively. Dry matter intake was not affected by APG supplementation. There was an increase in milk yield (from 13.96 to 16.71 kg/day) and increases in milk fat (quadratic, p = 0.04), protein (quadratic, p = 0.10), and lactose concentrations (linear, p = 0.07) with increasing APG supplementation. In addition, APG supplementation increased (p &le, 0.03) the milk fat, protein, solid non-fat, and total solid yields, while the lactose yield increased (linear, p = 0.01) as the APG level increased. Dietary APG supplementation had no effect on nutrient digestibility and blood metabolites. It was concluded that the addition of APG at doses up to 22 mL/kg of pelleted concentrate had positive effects on the milk composition in dairy cows.

BACKGROUND Post-ruminal digestion of fiber has received much less attention than its ruminal digestion. Using in vitro incubation techniques, the present study explored whether variations in fiber digestion occurred in different segments of the post-ruminal tract and whether fiber structure could influence its digestibility. A split plot design was conducted with gut segments (jejunum, ileum, cecum and colon) as main plot and substrates (neutral detergent fiber (NDF) and cellulose (CEL)) as subplot. RESULTS With the same substrate, the final asymptotic gas volume (VF), gas production at ti (), digestibility, microbial crude protein (MCP), total bacteria number (TBN), total short-chain fatty acids (TSCFA) and xylanase in incocula from the cecum and colon exceeded (P < 0.01) those in incocula from the jejunum and ileum, while the NH3-N in the former was less (P < 0.01). For the same gut segment, the digestion of CEL was superior to NDF, as reflected in greater VF, , maximum rate of gas production, digestibility, enzyme activities and SCFA but lower pH and NH3-N. CONCLUSION The current results imply that the intestinal contents from the cecum and colon have greater potential to digest fiber than those from the jejunum and ileum, and CEL is more easily digested in the post-ruminal tract than NDF. © 2013 Society of Chemical Industry

Post-ruminal digestion of fiber has received much less attention than its ruminal digestion. Using in vitro incubation techniques, the present study explored whether variations in fiber digestion occurred in different segments of the post-ruminal tract and whether fiber structure could influence its digestibility. A split plot design was conducted with gut segments (jejunum, ileum, cecum and colon) as main plot and substrates (neutral detergent fiber (NDF) and cellulose (CEL)) as subplot.With the same substrate, the final asymptotic gas volume (V(F)), gas production at t(i) (V(t(i)), digestibility, microbial crude protein (MCP), total bacteria number (TBN), total short-chain fatty acids (TSCFA) and xylanase in incocula from the cecum and colon exceeded (P0.01) those in incocula from the jejunum and ileum, while the NH3-N in the former was less (P0.01). For the same gut segment, the digestion of CEL was superior to NDF, as reflected in greater V(F), V(t(i)), maximum rate of gas production, digestibility, enzyme activities and SCFA but lower pH and NH3-N.The current results imply that the intestinal contents from the cecum and colon have greater potential to digest fiber than those from the jejunum and ileum, and CEL is more easily digested in the post-ruminal tract than NDF.

The objective of this study was to examine the effects of maternal protein or energy restriction during late gestation on postnatal fetal growth and visceral organ mass of goats. Eighty pregnant goats with similar age (2.0 ± 0.3 yr) and body weight (BW, 20.0 ± 1.0 kg before pregnancy) were assigned to 3 dietary treatments during late gestation: control (CON), 40% protein restricted (PR) and 40% energy restricted (ER) diets until parturition, after which offspring received normal diets for nutritional recovery. Kids were killed and visceral tissues were harvested at birth and week 6. Maternal protein or energy restriction decreased (P0.05) birth weight, and the weights of thymus, heart, abomasums, small intestine. The length of fetus from PR and ER were all decreased (P0.05) compared with that from control. When expressed relative to BW, thymus and small intestine for PR and ER still remained less (P0.05) than that for control. After 6 weeks of nutritional recovery, there was no difference (P = 0.91) in BW among groups; the kids from nutritional restriction groups showed a greater (P0.05) growth rate compared with kids from CON. Moreover, liver (only in ER, P0.10) and kidney (only in ER, P0.05) were proportionally increased to BW at week 6. The results indicate that maternal protein or energy restriction programs the fetal growth in goats, particularly the proportional responses of fetal organs relative to BW, including thymus, small intestine, kidney and liver.