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Objective: To explore the effects of matrine on HCCR1 and HCCR2 expression in cultural human hepatocellular carcinomas (HCC) cells at the level of gene and protein. Methods: Three methods, representational difference analysis (RDA) of cDNA, microarrays and fluorescence in situ hybridization (FISH) were used to detect levels of mRNA and protein expression of HCCR1 and HCCR-2 before and after treatment of matrine. Results: Matrine had inhibitory effect on the mRNA and protein expression of HCCR1 and HCCR2 in cultural HCC cells. Conclusion: Matrine has inhibitory effect on gene transcription, protein expression of HCCR1 and HCCR2 in cultural HCC cells.

Objective: To identify aberrant gene expression in primary human T-cell acute lymphoblastic leukemia (T-ALL) and to evaluate the differently expressed level of Lyl1 and LMO2 genes in human LMO2 positive/TAL1 negative T-ALL tumors. Methods: Three methods, representational difference analysis (RDA) of cDNA, cDNA microarrays and RT-PCR were used to detect if Lyl1 and LMO2 genes were differently expressed in human LMO2 positive/tal1 negative T-ALL tumors. Results: The results of cDNA RDA and cDNA array shown that Lyl1 and LMO2 genes are differently expressed in human T-cell tumors. The result of RT-PCR also shown Lyl1 and LMO2 are high expressed in human T-cell tumors and very low level or no expressed in normal group. Conclusion: We have found that cDNA RDA and cDNA microarray can be successfully used to identify aberrant gene expression in T-ALL cells. In the study described in this manuscript we found that Lyl1 and LMO2 are aberrantly expressed in human T-ALL LMO2 positive/TAL1 negative T-ALL tumors.

Prostate cancer is the most frequently diagnosed cancer in the Western male population and the second leading cause of cancer mortality. Recently many new methods are used to detect the tumor, some of which use the urine as the sample. The biomarkers in the urine will possibly improve the sensitivity and specificity to aid in prostate cancer detection.