1. Raman spectroscopy and sciatic functional index (SFI) after low-level laser therapy (LLLT) in a rat sciatic nerve crush injury model
- Author
-
Melissa de Almeida Melo Maciel Mangueira, Egas Caparelli-Dáquer, Ozimo Pereira Gama Filho, Diogo Souza Ferreira Rubim de Assis, Janyeid Karla Castro Sousa, Willy Leite Lima, Antonio Luiz Barbosa Pinheiro, Landulfo Silveira, and Nilton Maciel Mangueira
- Subjects
Crush Injuries ,Nerve Crush ,Peripheral Nerve Injuries ,Animals ,Surgery ,Dermatology ,Low-Level Light Therapy ,Rats, Wistar ,Sciatic Neuropathy ,Spectrum Analysis, Raman ,Sciatic Nerve ,Nerve Regeneration ,Rats - Abstract
Axonotmesis causes sensorimotor and neurofunctional deficits, and its regeneration can occur slowly or not occur if not treated appropriately. Low-level laser therapy (LLLT) promotes nerve regeneration with the proliferation of myelinating Schwann cells to recover the myelin sheath and the production of glycoproteins for endoneurium reconstruction. This study aimed to evaluate the effects of LLLT on sciatic nerve regeneration after compression injury by means of the sciatic functional index (SFI) and Raman spectroscopy (RS). For this, 64 Wistar rats were divided into two groups according to the length of treatment: 14 days (n = 32) and 21 days (n = 32). These two groups were subdivided into four sub-groups of eight animals each (control 1; control 2; laser 660 nm; laser 808 nm). All animals had surgical exposure to the sciatic nerve, and only control 1 did not suffer nerve damage. To cause the lesion in the sciatic nerve, compression was applied with a Kelly clamp for 6 s. The evaluation of sensory deficit was performed by the painful exteroceptive sensitivity (PES) and neuromotor tests by the SFI. Laser 660 nm and laser 808 nm sub-groups were irradiated daily (100 mW, 40 s, energy density of 133 J/cm
- Published
- 2022