1. Lactobacillus paracasei ssp. paracasei YBJ01 reduced d-galactose-induced oxidation in male Kuming mice
- Author
-
Li Jian, Hongwei Wang, Jia-Le Song, Huayi Suo, Yu Zhang, Xin Zhao, Liu Shanchun, and Ding Yangping
- Subjects
0301 basic medicine ,Male ,Antioxidant ,Lactobacillus paracasei ,medicine.medical_treatment ,Antioxidants ,Superoxide dismutase ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,In vivo ,Malondialdehyde ,Genetics ,medicine ,Animals ,chemistry.chemical_classification ,Glutathione Peroxidase ,biology ,Superoxide ,Superoxide Dismutase ,Glutathione peroxidase ,Probiotics ,food and beverages ,Galactose ,Glutathione ,Lacticaseibacillus paracasei ,biology.organism_classification ,Catalase ,Yogurt ,Oxidative Stress ,030104 developmental biology ,chemistry ,Biochemistry ,Liver ,030220 oncology & carcinogenesis ,Fermentation ,biology.protein ,Animal Science and Zoology ,Cattle ,Oxidation-Reduction ,Food Science - Abstract
We investigated in vitro and in vivo antioxidant activity of Lactobacillus paracasei ssp. paracasei YBJ01 (LPSP-YBJ01) isolated and identified from fermented yogurt. Strain LPSP-YBJ01 had stress tolerance against acidity, bile salt, and osmotic pressure. Five in vitro antioxidant assays were used to evaluate antioxidant activity of LPSP-YBJ01, which could scavenge free radicals (2,2-diphenyl-1-picrylhydrazyl and hydroxyl) and superoxide anion in vitro. In addition, strain LPSP-YBJ01 had stronger antilipid peroxidation activity and weak reducing power in vitro. We measured in vivo antioxidant activity of LPSP-YBJ01 in an oxidation mouse model induced by d-galactose injection. Strain LPSP-YBJ01 significantly increased serum superoxide dismutase (SOD), glutathione peroxidase, and total-antioxidant capability, and inhibited generation of malondialdehyde in a dose-dependent manner. In addition, strain LPSP-YBJ01 also increased the hepatic and splenic protein expressions of some antioxidant enzymes such as catalase, Cu/Zn-SOD, and Mn-SOD in mice treated with d-galactose. Thus, LPSP-YBJ01 had antioxidant activity in vitro and in vivo and may be a useful probiotic.
- Published
- 2018