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Introduction Long chain Omega-3 polyunsaturated fatty acids, including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), have several anti-inflammatory, antioxidant, and lipid lowering properties and can promote brain health during pregnancy and early life. In recent years, a large number of systematic reviews and meta-analyses (SRMA) of randomized controlled trials (RCT) have been undertaken of the effects of supplementation with omega-3 polyunsaturated fatty acids in pregnancy, lactation, and infancy. There is no comprehensive umbrella review of the estimates of effects for omega-3 fatty acids with trial data, including the certainty of evidence for each estimate and the magnitude of the observed effect based on the minimal important difference. We will perform an umbrella review of SRMA of RCTs on the effects of supplementations with long chain omga-3 fatty acids on any health related outcomes in pregnancy, lactation, and infancy (

Two water-soluble nickel (II) Schiff base complexes were prepared and their interaction with fish sperm DNA (FS-DNA) was investigated by various methods including UV–vis spectroscopy, fluorescence spectroscopy, cyclic voltammetry, and viscometric measurements. Complex 1: [N,N′-bis{5-[(triphenyl phosphonium chloride)-methyl] salicylidine}-3,4-diaminobenzophenone]nickel(II) perchloride dihydrate: [Ni(5-CH2PPh3-3,4-salophen)] (ClO4)2.2 H2O was synthesized as a new complex and characterized by elemental analysis, IR, 1H NMR, thermal gravimetric analysis (TGA) and UV–vis spectroscopy. Complex 2: sodium [(N,N′-bis(5-sulfosalicyliden)-3, 4-diaminobenzophenone)aqua] nickel(II) hydrate: Na2[Ni (5-SO3-3,4-salbenz)(H2O)]. H2O was already synthesized by our research team, but in this study, its function as a DNA-binding compound was tested, and compared with the results of complex 1-DNA binding. The calculation of different constants using absorption and emission data, all confirmed the stronger binding ability of complex 1 than complex 2 with DNA. Different thermodynamic parameters showed the interactions between DNA and complexes were the type of hydrophobic interaction for complex 1 and electrostatic interaction for complex 2. Also, the negative values of free energy changes proved a spontaneous DNA binding process. Based on cell toxicity assay against two different cell lines including Jurkat and MCF-7, the effect of complex 1 was comparable to cisplatin, and the toxicity mechanism was further justified by bright field microscopy, flow cytometry, and cleavage of DNA in the presence of H2O2. Besides, the docking calculations suggested intercalation after measuring the lowest-energy between the complexes and DNA. For both complexes, all analytical, spectroscopic, and molecular modeling methods supported partial intercalation as the main binding mode between the complexes and DNA.

The main purposes of this study are to distinguish the trends of research in publication exits for the utilisations of the fuzzy expert and knowledge-based systems that is done based on the classification of studies in the last decade. The present investigation covers 60 articles from related scholastic journals, International conference proceedings and some major literature review papers. Our outcomes reveal an upward trend in the up-to-date publications number, that is evidence of growing notoriety on the various applications of fuzzy expert systems. This raise in the reports is mainly in the medical neuro-fuzzy and fuzzy expert systems. Moreover, another most critical observation is that many modern industrial applications are extended, employing knowledge-based systems by extracting the experts' knowledge.