Search

To automatically collect information from the Internet and provide it to users for query, much of the existing search engines have been focused on massive and sprawling information. However, we note that the search engines produce a host of answers to specific questions with little accuracy or intelligence. Therefore, we consider the approach of question answering (QA) systems based on the knowledge graph (KG). Resent works are widely shared that the KG can be deeper for the field of medicine and finance, etc. Nowadays, QA system in the military is sorely needed with the dramatic growth of the technology. In this paper, we propose a QA system based on the KG in the military (QAM). Which applies semantic Web technology, and allows a deep analysis of military questions and documents at both representation and interrogation levels. The experimental results reveal that KG applied in the military domain, can make up the inadequacy of the general search engine, return to refine and accurate results, and provide conveniently knowledge service in the military field.

Background Capripox viruses are economically important pathogens in goat and sheep producing areas of the world, with specific focus on goat pox virus (GTPV), sheep pox virus (SPPV) and the Lumpy Skin Disease virus (LSDV). Clinically, sheep pox and goat pox have the same symptoms and cannot be distinguished serologically. This presents a real need for a rapid, inexpensive, and easy to operate and maintain genotyping tool to facilitate accurate disease diagnosis and surveillance for better management of Capripox outbreaks. Results A LAMP method was developed for the specific differential detection of GTPV and SPPV using three sets of LAMP primers designed on the basis of ITR sequences. Reactions were performed at 62°C for either 45 or 60 min, and specificity confirmed by successful differential detection of several GTPV and SPPV isolates. No cross reactivity with Orf virus, foot-and-mouth disease virus (FMDV), A. marginale Lushi isolate, Mycoplasma mycoides subsp. capri, Chlamydophila psittaci, Theileria ovis, T. luwenshuni, T. uilenbergi or Babesia sp was noted. RFLP-PCR analysis of 135 preserved epidemic materials revealed 48 samples infected with goat pox and 87 infected with sheep pox, with LAMP test results showing a positive detection for all samples. When utilizing GTPV and SPPV genomic DNA, the universal LAMP primers (GSPV) and GTPV LAMP primers displayed a 100% detection rate; while the SPPV LAMP detection rate was 98.8%, consistent with the laboratory tested results. Conclusions In summary, the three sets of LAMP primers when combined provide an analytically robust method able to fully distinguish between GTPV and SPPV. The presented LAMP method provides a specific, sensitive and rapid diagnostic tool for the distinction of GTPV and SPPV infections, with the potential to be standardized as a detection method for Capripox viruses in endemic areas.