26,156 results on '"DENTIN"'
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2. Efecto de diferentes condiciones y modos de aplicación sobre la fuerza de unión de los adhesivos a la dentina
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Cestari Fagundes, Ticiane, Simões Gonçalves, Rafael, de Souza e Silva Ramos, Fernanda, de Castro Oliveira, Laryssa, Dias Moda, Mariana, and Sanae Shinohara, Mirela
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Dentin-Bonding Agents ,Tensile Strength ,Ultrasonido ,Dentin ,Ultrasonics ,Resistencia a la Tracción ,General Medicine ,Dentina ,Recubrimientos Dentinarios - Abstract
Objective: To evaluate the microtensile bond strength in different dentine conditions (etched-E, non-etched-N, dry-D and wet-W) of a multimode adhesive (Scotchbond Universal-SU, 3M/ESPE) and a total etching adhesive (Ambar-AB, FGM) using a sonic device (Smart Sonic Device-SD, FGM). Material and methods: In this in vitro study, ninety six sound extracted human molars were divided into 12 groups (n=8) according to different dentine conditions and adhesive systems. Enamel was removed and the middle dentine surfaces were polished. Each adhesive system was applied according to the different dentine conditions, and composite resin blocks were incrementally built up and stored for 24 hours. Specimens were sectioned into sticks and bond strength data were analyzed with the Kruskal-Wallis test and Mann-Whitney U tests. Results: No effects of sonic application and were observed. In general, AB showed lower results compared to the SU. E and N conditions did not statistically affect the bond strength of SU groups. Dry dentine presented statistically superior bond strength values when compared to wet dentine for SU/E/SD group. Conclusion: Adhesion of dry dentine with multimode adhesive system may be superior to wet dentine with sonic application. The modes of application had no influence in bond strength of studied adhesives. RESUMEN Objetivo: Evaluar la resistencia de la unión microtensil en diferentes condiciones de dentina (grabado-E, sin grabado-N, seco-D y húmedo-W) de un adhesivo multimodo (Scotchbond Universal-SU, 3M/ESPE) y un adhesivo de grabado total (Ambar-AB, FGM) utilizando un dispositivo sónico (Smart Sonic Device-SD, FGM). Material y Métodos: En este estudio in vitro, noventa y seis molares humanos extraídos sanos se dividieron en 12 grupos (n=8) de acuerdo con diferentes condiciones de dentina y sistemas adhesivos. Se eliminó el esmalte y se pulieron las superficies centrales de la dentina. Cada sistema adhesivo se aplicó de acuerdo con las diferentes condiciones de dentina, y los bloques de resina compuesta se acumularon de forma incremental y se almacenaron durante 24h. Las muestras se seccionaron en barras y los datos de resistencia de la unión se analizaron con la prueba de Kruskal-Wallis y la prueba de U de Mann-Whitney. Resultado: No se observaron efectos de la aplicación sónica. En general, AB mostró resultados más bajos en comparación con el SU. Las condiciones E y N no afectaron estadísticamente la fuerza de unión de los grupos SU. La dentina seca presentó valores de fuerza de adhesión estadísticamente superiores en comparación con la dentina húmeda para el grupo SU/E/SD. Conclusión: La adhesión de la dentina seca con un sistema adhesivo multimodo puede ser superior a la dentina húmeda con aplicación sónica. Los modos de aplicación no tuvieron influencia en la resistencia de la unión de los adhesivos estudiados.
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- 2023
3. Evaluation of the sealing capability and morphological fit of prefabricated dowels and fiber-reinforced composite resin: An in vitro study
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Amaury de Jesús Pozos Guillén, Alicia Grajales Lagunes, Gabriel Fernando Romo Ramírez, Ana I Romo Tobías, Norma Verónica Zavala Alonso, Diana María Escobar García, and Marine Ortiz Magdaleno
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Materials science ,biology ,Bond strength ,Root canal ,Composite number ,technology, industry, and agriculture ,Fiber-reinforced composite ,Dowel ,Gutta-percha ,biology.organism_classification ,medicine.anatomical_structure ,stomatognathic system ,Dentin ,medicine ,Adhesive ,Oral Surgery ,Composite material - Abstract
Prefabricated dowels do not always provide intraradicular sealing in the root canal dentin, and the lack of sealing predisposes the dowel to adhesive failure and debonding. It is unclear if fiber-reinforced composite resin dowels provide better sealing.The purpose of this in vitro study was to evaluate the intraradicular sealing and morphological fit of prefabricated dowels and fiber-reinforced composite resin dowels in root canal dentin. The thickness of the resin cement layer and push-out bond strength were determined to assess their effects on the sealing of the dowels.A total of 50 permanent maxillary central incisors were endodontically treated and divided into 2 subgroups (n=25). In one group, prefabricated dowels were cemented; in the second group, fiber-reinforced composite resin dowels were placed. The thickness of the resin cement layer was assessed in 3 different locations: coronal, middle, and apical of the root canal dentin with fluorescence confocal laser microscopy. The push-out bond strength was then determined, and intraradicular sealing observed by using scanning electron microscopy.Fiber-reinforced composite resin dowels showed a closer intraradicular fit and seal in the root canal dentin, and the morphology of the apical portion of the fiber-reinforced composite resin dowels showed a sealing area with the gutta percha in the apical portion. The mean thickness of the resin cement layer was significantly reduced for the fiber-reinforced composite resin dowels compared with the prefabricated dowels (P.05) in the cervical area (197.0 μm versus 311.0 μm) and in the apical portion of the root canal (57.3 μm versus 131.6 μm). The mean push-out strength was higher for the fiber-reinforced composite resin dowels (22.98 N/mmThe morphological fit of fiber-reinforced composite resin dowels provides better intraradicular sealing in the cervical and apical portions, reducing the resin cement thickness. The increased push-out strength can therefore be assumed to result from increased frictional retention compared with prefabricated dowels.
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- 2023
4. Fatigue survival of endodontically treated teeth restored with different fiber-reinforced composite resin post strategies versus universal 2-piece fiber post system: An in vitro study
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Guilherme Schmitt de Andrade, Rodrigo Máximo de Araújo, João M. Silva, Alexandre Luiz Souto Borges, Gabriel Kalil Rocha Pereira, Marcela Moreira Penteado, and Luiz Felipe Valandro
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Materials science ,medicine.medical_treatment ,Glass fiber ,Composite number ,Ferrule ,Fiber-reinforced composite ,Crown (dentistry) ,Stress (mechanics) ,medicine.anatomical_structure ,Dentin ,medicine ,Fiber ,Oral Surgery ,Composite material - Abstract
Statement of problem Various strategies have been proposed to reduce the cement space of foundation restorations for endodontically treated teeth. However, they may add more operative steps, or the dentist must keep different sizes of drills and posts in stock. A 2-piece universal adjustable post system has been developed to overcome this problem, but whether the system has acceptable fatigue survival performance is unclear. Purpose The purpose of this in vitro study was to evaluate the fatigue survival and stress distribution of endodontically treated teeth without a ferrule and restored with different glass fiber post strategies versus a recently introduced universal 2-piece fiber post system. Material and methods Bovine incisor roots were randomly assigned to 3 groups as per the post used (n=13): adapted glass fiber post with post space preparation of the same size, composite resin-custom glass fiber post (CTM), and universal 2-piece glass fiber-reinforced composite resin post (UNI). The posts were adhesively luted, the composite resin core was added, and a composite resin crown was produced with computer-aided design and computer-aided manufacturing (CAD-CAM), and then adhesively luted to each core. A fatigue test was performed with the stepwise stress method (10 000 cycles/step; 20 Hz; load=100 N to 750 N; step=50 N) until fracture, and the failure mode analyzed. The stress distribution was evaluated by finite element analysis with the maximum principal stress criteria by following the parameters of the in vitro test. The solids were considered homogeneous, linear, and isotropic, except for the glass fiber post (orthotropic), and a load of 450 N at 30 degrees was applied. The fatigue failure load and the number of cycles for failure were analyzed with Kaplan-Meier and Mantel-Cox (log rank test) (α=.05). The finite element analysis results were analyzed with colorimetric graphs. Results The highest fatigue failure load and the number of cycles for failure were found in the UNI system, whereas the lowest results were found in the CTM group. All groups exhibited repairable failures. The finite element analysis showed the lowest stress in root dentin in the UNI system. The CTM system had the largest stress regions at the dentin and dentin-core interface. Conclusions The use of a 2-piece universal glass fiber post system resulted in more fatigue behavior compared with composite resin-custom glass fiber posts.
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- 2023
5. Efecto del tamaño de vóxel de micro-CT en la evaluación de la preparación del conducto radicular
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Pinto, Jader Camilo, Lucas-Oliveira, Everton, Bonagamba, Tito Jose, Guerreiro-Tanomaru, Juliane Maria, and Tanomaru-Filho, Mario
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Cavidad de la pulpa dental ,Instrumentos de níquel y titanio ,Root canal preparation ,Building and Construction ,Dental pulp cavity ,Microtomografía de rayos X ,Endodontics ,Endodoncia ,Dentina ,Preparación de Conductos radiculares ,X-ray microtomography ,Dentin ,Root canal ,Electrical and Electronic Engineering - Abstract
The aim of this study was to assess the influence of micro-computed tomography (micro-CT) voxel size on evaluation of root canal preparation using rotary heat-treated nickel-titanium files. Curved mesial root canals of mandibular molars were prepared using ProDesign Logic 30/.05 (PDL) or HyFlex EDM 25/.08 (HEDM) (n=12). The specimens were scanned using micro-CT with 5μm of voxel size before and after root canal preparation. Images with sub-resolution of 10 and 20μm voxel sizes were obtained. The percentage of volume increase, debris and uninstrumented root canal surface were analyzed in the different voxel sizes. Data were compared using unpaired Student’s t-test and ANOVA statistical tests (α=0.05). No differences were observed for percentage of volume increase, debris and instrumented surface between the root canals prepared by PDL and HEDM (p>0.05). Both systems promoted higher percentage of debris in the apical third compared to the middle third (p0.05). PDL and HEDM had similar root canal preparation capacity. Micro-CT images using different voxel sizes did not influence the results of volume increase and debris evaluation. However, images at 5µm showed greater accuracy to evaluate the percentage of uninstrumented surfaces. El objetivo de este estudio fue evaluar la influencia del tamaño de vóxel de la microtomografía computarizada (micro-CT) en la evaluación de la preparación del conducto radicular utilizando limas rotatorias de níquel-titanio tratadas térmicamente. Se prepararon conductos radiculares mesiales curvos de molares mandibulares usando ProDesign Logic 30/.05 (PDL) o HyFlex EDM 25/.08 (HEDM) (n=12). Las muestras se escanearon usando micro-CT con un tamaño de vóxel de 5μm antes y después de la preparación del conducto radicular. Se obtuvieron imágenes con subresolución de vóxeles de 10 y 20μm. Se analizó el porcentaje de aumento de volumen, residuos y superficie del conducto radicular no instrumentado en diferentes tamaños de vóxel. Los datos se compararon usando la prueba t de Student no pareada y las pruebas estadísticas ANOVA (α=0,05). No se observaron diferencias en el porcentaje de aumento de volumen, detritus y superficie instrumentada entre los conductos radiculares preparados por PDL y HEDM (p>0,05). Ambos sistemas promovieron un mayor porcentaje de detritos en el tercio apical en comparación con el tercio medio (p0,05). PDL y HEDM tenían una capacidad de preparación del conducto radicular similar. Las imágenes de micro-CT que utilizan diferentes tamaños de vóxel no influyeron en los resultados de la evaluación del volumen y los desechos. Sin embargo, las imágenes de 5µm mostraron una mayor precisión al evaluar el porcentaje de superficies no instrumentadas.
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- 2023
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6. Dexamethasone and zinc loaded polymeric nanoparticles reinforce and remineralize coronal dentin. A morpho-histological and dynamic-biomechanical study
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Manuel, Toledano, Estrella, Osorio, Fátima S, Aguilera, María T, Osorio, Raquel, Toledano, Modesto T, López-López, Christopher D, Lynch, and Raquel, Osorio
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Viscoelastic ,Zinc ,Mechanics of Materials ,Dentin ,dexamethasone ,Transmission Electron Microscopy ,General Materials Science ,Apatite ,General Dentistry ,Remineralization ,Polymeric nanoparticles ,X-ray diffraction - Abstract
Objective: To investigate the effect of novel polymeric nanoparticles (NPs) doped with dexamethasone (Dex) on viscoelasticity, crystallinity and ultra-nanostructure of the formed hydroxyapatite after NPs dentin infiltration. Methods: Undoped-NPs, Dex-doped NPs (Dex-NPs) and zinc-doped-Dex-NPs (Zn-Dex-NPs) were tested at dentin, after 24 h and 21 d. A control group without NPs was included. Coronal dentin surfaces were studied by nano-dynamic mechanical analysis measurements, atomic force microscopy, X-ray diffraction and transmission electron microscopy. Mean and standard deviation were analyzed by ANOVA and Student-Newman-Keuls multiple comparisons (p < 0.05). Results: At 21 d of storage time, both groups doped with Dex exhibited the highest complex, storage and loss moduli among groups. Zn-Dex-NPs and Dex-NPs promoted the highest and lowest tan delta values, respectively. Dex-NPs contributed to increase the fibril diameters of dentin collagen over time. Dentin surfaces treated with Zn-Dex-NPs attained the lowest nano-roughness values, provoked the highest crystallinity, and produced the longest and shortest crystallite and grain size. These new crystals organized with randomly oriented lattices. Dex-NPs induced the highest microstrain. Crystalline and amorphous matter was present in the mineral precipitates of all groups, but Zn and Dex loaded NPs helped to increase crystallinity. Significance: Dentin treated with Zn-Dex-NPs improved crystallographic and atomic order, providing structural stability, high mechanical performance and tissue maturation. Amorphous content was also present, so high hydroxyapatite solubility, bioactivity and remineralizing activity due to the high ion-rich environment took place in the infiltrated dentin., This work was funded by Grant PID2020-114694RB-I00 funded by MCIN/AEI 10.13039/501100011033.
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- 2023
7. Construction of an antibacterial low-defect hybrid layer by facile PEI electrostatic assembly promotes dentin bonding
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Yuqing Lei, Jiajia Xu, Mengqi Pan, Yadong Chen, Xiaojun Li, Weipu Zhu, Chang Shu, Tianxiang Fang, Hongbing Liao, Qiaojie Luo, and Xiaodong Li
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Static Electricity ,Materials Testing ,Dentin ,Stomatognathic Diseases ,Biomedical Engineering ,Humans ,Polyethyleneimine ,Biocompatible Materials ,General Materials Science ,General Chemistry ,General Medicine ,Anti-Bacterial Agents - Abstract
Dentin bonding is the most common form of human tissue repair among tissue-biomaterial adhesions, concerning billions of people's oral health worldwide. However, insufficient adhesive infiltration in the demineralized dentin matrix (DDM) always produces numerous defects in the bonding interface termed the hybrid layer, which causes high levels of bacteria-related secondary dental diseases, and less than 50% of the bonding lasts more than 5 years. Therefore, it is urgent and vital to construct an antibacterial low-defect hybrid layer to solve the durability-related problems. A DDM with a hydrogel-like surface formed by the hydration of highly-anionic non-collagenous proteins (NCPs) is firstly used as a template to electrostatically assemble polyethyleneimine (PEI). The formation of a stable antibacterial polyelectrolyte complex of PEI/NCPs rapidly eliminates NCP hydration capacity and significantly improves the infiltration of various adhesives. Simultaneously, both the PEI during the assembly and the PEI-assembled DDM can directly destroy a biofilm of
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- 2023
8. Salivary esterases dramatically reduce biostability of dentin collagen treated with galloylated polyphenols
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Viviane Hass, Yong Li, Saleha Nisar, Zhonghua Peng, and Yong Wang
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Molecular Weight ,Mechanics of Materials ,Dentin ,Esterases ,Humans ,Polyphenols ,General Materials Science ,Collagen ,General Dentistry - Abstract
To investigate the effects of salivary esterases on biostability of collagen treated by galloylated polyphenols.Human dentin was microtomed into 6-μm-thick films, which were demineralized and treated for 60 s using solutions containing 0.6% and 2% of one of the crosslinkers: tannic acid (TAC), epigallocatechin gallate (EGCG), epigallocatechin (EGC), and N-[3-dimethylaminopropyl]-N'-ethylcarbodiimide hydrochloride (EDC)/N-hydroxysuccinimide (NHS), and for 1 h using EDC/NHS. Half of the treated and untreated (control) films were subjected to human saliva incubation. Collagen biostability was assessed via exogenous protease biodegradation by weight loss and hydroxyproline release, and endogenous MMPs by in situ zymography. The degradation products of galloylated polyphenols (TAC and EGCG) by saliva were monitored using proton nuclear magnetic resonance (Collagen films treated with TAC and EGCG exhibited significantly improved biostability (p 0.05); however, the enhanced biostability was severely reduced after saliva incubation (p 0.001). For EDC/NHS treated collagen, saliva incubation showed negligible effect on the biostability.The galloyl group plays important role in collagen crosslinking, inducing higher biostability. However, galloylated polyphenols crosslinked on collagen are highly susceptible to metabolism of human saliva by salivary esterase, dramatically compromising the enhanced biostability.
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- 2022
9. Should statistical analysis of bond-strength data include or exclude cohesive failures?
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Franz, A., Lettner, S., Watts, D.c., and Schedle, A.
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Mechanics of Materials ,Dentin-Bonding Agents ,Dentin ,Materials Testing ,Dental Bonding ,Animals ,Dental Cements ,Cattle ,General Materials Science ,Shear Strength ,Composite Resins ,General Dentistry ,Resin Cements - Abstract
ObjectivesThe aim was to investigate shear bond strengths and failure modes of four self-etch bonding agents to bovine dentin and enamel and to compare evaluation of data sets with or without exclusion of cohesive failure specimens.MethodsComposite-cylinders were bonded perpendicularly to bovine dentin and enamel surfaces. Shear-strengths were measured 24 h post-bonding of: Scotchbond Universal® (SBU, 3 M), OptiBond™ XTR (OBXTR, Kerr), OptiBond™ universal (OBU, KaVo-Kerr) and Prime & Bond active® (PBA, Dentsply-Sirona). Analysis of overall data was made via a linear mixed-model. This was repeated after exclusion of specimens associated with cohesive failures.ResultsWhen both adhesive and cohesive failures were considered, OBU and OBXTR showed comparable dentin and enamel bond strengths, whereas lower strengths were found on enamel for SBU (p < 0.001) and PBA (p = 0.015). For OBXTR higher shear strengths were measured for specimens associated with cohesive failures. When cohesive failures were excluded, the majority of shear bond strengths of adhesive failure specimens were only slightly different from overall results. However, uniquely with OBXTR dramatically lower shear bond strengths were found for dentin substrate.SignificanceAfter exclusion of cases with cohesive failures OBXTR adhesive fell behind other materials in the sequence of average shear strengths. This did not reflect the actual performance of the material. Therefore, in statistical analysis we do not recommend exclusion of data based on a specific fracture mode.
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- 2022
10. Dentine biomodification by sulphonamides pre-treatment: bond strength, proteolytic inhibition, and antimicrobial activity
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Maristela Barbosa Portela, Caroliny Mello Barboza, Eduardo Moreira da Silva, Daniel Clemente de Moraes, Renata Antoun Simão, Clara Ribeiro de Souza, Verônica da Silva Cardoso, Antônio Ferreira-Pereira, Alane Beatriz Vermelho, and Claudiu T. Supuran
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Pharmacology ,Sulfonamides ,Anti-Infective Agents ,Dentin ,Spectroscopy, Fourier Transform Infrared ,Drug Discovery ,Humans ,Collagen ,General Medicine - Abstract
We evaluated the effects of dentine biomodification after pre-treatment with two sulphonamide carbonic anhydrase inhibitors (CAIs) of the N-[4-sulphamoylphenethylcarbamoyl]benzenesulphonamide type, investigating matrix metalloproteases activity, resin-dentine micro tensile bond strength, dentine surface wettability, and antimicrobial activities. Ninety-five sound-extracted human molars were selected for the study. Inhibitory effects were evaluated by gelatinase and collagenase activity tests and collagen degradation FT-IR spectroscopic analysis. Pre-treatment with the two CAIs kept the micro tensile values after 12 months of storage (32.23 ± 5.95) and cariogenic challenge (34.13 ± 2.71) similar to the initial, pre-treatment values (33.56 ± 4.34). A decreased
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- 2022
11. Effect of bioactive glasses used as dentin desensitizers on the dentin-pulp complex in rats
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Ana Cláudia, Dalmolin, Luisa Fernanda Alegria, Acevedo, Letícia Antonelo, Campos, Iolanda Cristina Justus, Dechandt, Francisco Carlos, Serbena, Edgar Dutra, Zanotto, Márcia Thaís, Pochapski, César Augusto Galvão, Arrais, Eduardo Bauml, Campagnoli, and Fábio André Dos, Santos
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Male ,Dentin ,Ceramics and Composites ,Animals ,Gingival Recession ,Dentin Sensitivity ,Rats, Wistar ,Substance P ,General Dentistry ,Rats - Abstract
Bioactive glasses have been recommended for the occlusion of dentinal tubules in treating cervical dentin hypersensitivity. This study evaluates an in vivo model of dentin exposure, and tests the efficacy of bioglass treatments. Thirty male Wistar rats received gingival recession surgery on the upper left first molar. The treatments were applied over the surface of the exposed dentin every 4 days for 28 days. The groups were as follows: Naive; Gingival recession; Cavity varnish; Biosilicate
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- 2022
12. Cytotoxic Evaluation of the New Composite Resin through an Artificial Pulp Chamber
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Luca Marigo, Alessio Triestino, Raffaella Castagnola, Federica Vincenzoni, Massimo Cordaro, Enrico Di Stasio, Alvaro Mordente, and Giuseppina Nocca
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Article Subject ,General Immunology and Microbiology ,General Medicine ,Composite Resins ,General Biochemistry, Genetics and Molecular Biology ,cytotoxicity ,atificial pulp chamber ,Mice ,Italy ,Germany ,Dentin ,Animals ,Dental Pulp Cavity ,Settore BIO/10 - BIOCHIMICA - Abstract
The aim of this study was to analyse the cytocompatibility of Surefil One (SuO) with respect to the release of monomers from the material. The following reference materials were chosen: SDR Flow Plus (SDR, Dentsply Sirona, Konstanz, Germany), One Q Bond (Q, Dentalica, Milan, Italy), and Ketac (K, 3M-ESPE, USA). Fifteen dentin discs (2 mm thickness and diameter) were obtained from 15 third molars and were used in this study. After dentin disc permeability measurement, murine fibroblasts were grown, and the pulp surface of the dentinal disc was placed in direct contact with the cells immersed in DMEM. The experimental materials were positioned on the occlusal side of each dentinal disc until a uniform thickness of 2 mm was obtained. Then, the discs were inserted into an artificial pulp chamber for 24 hours to assess the cytocompatibility. Afterwards, the moles of monomers leached from the specimens in DMEM were determined using HPLC. Statistical analysis was performed using ANOVA ( p < 0.05 ). Under the experimental conditions, the toxic effect induced by all tested materials was slight or absent. Diurethane dimethacrylate and acrylic acid were not found in the culture media. It is concluded that all materials have good cytocompatibility consistent with the nondeterminability of the monomers released after polymerization.
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- 2022
13. Effect of the Viscosity and Application Time of Different Phosphoric Acids on the Bond Strength and Nanoleakage of Fiber Posts to Root Dentin
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R, Ñaupari-Villasante, J B, Ocampo, Afm, Cardenas, L P, Muniz, Fsf, Siqueira, Acl, Moreira, V P, Feitosa, and A D, Loguercio
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Viscosity ,Dentin-Bonding Agents ,Dentin ,Materials Testing ,Dental Bonding ,Phosphoric Acids ,Dental Pulp Cavity ,General Dentistry ,Post and Core Technique ,Resin Cements - Abstract
SUMMARY Purpose To evaluate the effect of application time and viscosity of meta-phosphoric acid (MPA) and ortho-phosphoric acid (OPA) on the push-out resin-dentin bond strength (PBS) and nanoleakage (NL) at the adhesive-root dentin interface. Methods and Materials Ninety-six roots of premolars were endodontically prepared and randomly assigned into groups according to the (i) acid (OPA and MPA), (ii) viscosity (gel and liquid), and (iii) application time (7 and 15 seconds). Fiber posts were cemented to the roots, which were then transversally sectioned into serial slices. The slices (cervical, medium, and apical) were subjected to PBS or NL. Analysis of the dentin etching pattern was performed using scanning electron microscopy (SEM). The PBS (MPa) and NL (%) data were subjected to three-way repeated-measures analysis of variance (ANOVA) and Tukey’s test (α=0.05). Results The cross-product interaction was significant for both PBS and NL (p0.05). Higher PBS values were observed in the groups etched for 15 seconds compared to 7 seconds, regardless of the acid, viscosity, or root third (p0.05). A more pronounced etching pattern was observed with OPA than MPA regardless of the acid viscosity. Conclusion The use of 40% meta-phosphoric acid promoted adequate bond strength without increasing dentin demineralization or void spaces in the hybrid layer.
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- 2022
14. Bond strength of self-adhesive flowable composite resins to dental tissues: A systematic review and meta-analysis of in vitro studies
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Carlos Enrique Cuevas-Suárez, Carla David, Evandro Piva, Rafael R. Moraes, Cristina Pereira Isolan, and Gabriela Cardoso de Cardoso
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Materials science ,Surface Properties ,Composite number ,Dental Cements ,Composite Resins ,03 medical and health sciences ,0302 clinical medicine ,stomatognathic system ,Materials Testing ,Adhesive system ,Flowable Composite ,Dentin ,medicine ,Composite material ,Enamel paint ,Bond strength ,Dental Bonding ,technology, industry, and agriculture ,030206 dentistry ,Resin Cements ,medicine.anatomical_structure ,Self adhesive ,Dentin-Bonding Agents ,visual_art ,visual_art.visual_art_medium ,Adhesive ,Oral Surgery - Abstract
Although the clinical use of self-adhesive flowable composite resins is convenient, the decision to use flowable or conventional composite resin remains controversial.The purpose of this systematic review and meta-analysis was to assess the in vitro bond strength of self-adhesive flowable composite resins to enamel or dentin compared with conventional composite resins using etch-and-rinse or self-etch adhesive systems.Two independent reviewers performed searches in the PubMed (MEDLINE), Cochrane Wiley, Web of Science, Scopus, and Google Scholar databases for studies reporting on the bond strength of self-adhesive flowable and conventional composite resins to enamel and dentin published from January 2010 up to September 2020. A meta-analysis software program was used for the meta-analysis. Comparisons were conducted using standardized mean differences considering the random-effects model (α=.05).Twenty-two studies were considered for the meta-analysis. Immediate and long-term bond strength to enamel was improved when a conventional composite resin was used in combination with an adhesive system (P.001). Bond strength to dentin was improved by using an adhesive system in combination with a conventional composite resin (P.001).The bond strength of self-adhesive flowable composite resins is lower than that of conventional composite resins, indicating limited ability to bond to enamel and dentin.
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- 2022
15. Glass-ionomer and calcium silicate-based cements interactions with human dentine in health and disease: Two-photon fluorescence microscopy and Raman spectroscopy analysis
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Shara Sajini, Amre R. Atmeh, Avijit Banerjee, Frederic Festy, Richard J. Cook, Manoharan Andiappan, and Timothy F. Watson
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Minerals ,Silicates ,Acrylic Resins ,Calcium Compounds ,Dental Caries ,Silicon Dioxide ,Spectrum Analysis, Raman ,Phosphates ,Microscopy, Fluorescence ,Glass Ionomer Cements ,Tetracyclines ,Mechanics of Materials ,Dentin ,Humans ,General Materials Science ,General Dentistry - Abstract
To investigate the potential mineralising effects of calcium silicate-based dentine replacement material (Biodentine™) in comparison with glass-ionomer cement (GIC) (Fuji IX™) on different human dentine substrates using a multimodal non-invasive optical assessment.Cements were applied on artificially demineralised or naturally carious dentine and stored for 4 weeks in phosphate-rich media +/- tetracycline used for mineralisation labelling. Interfacial dentine was examined from the same sample and location before and after aging using two-photon fluorescence microscopy, fluorescence lifetime imaging (FLIM) and second harmonic generation (SHG) imaging. Additionally, Raman spectroscopy was used to detect changes in the mineral content of dentine.Significant changes in the fluorescence intensity and lifetime were detected in partially demineralised dentine and caries-affected dentine underneath both tested cements, after storage (p 0.001). This was associated with a significant increase in the mineral content as indicated by the increased intensity of the phosphate Raman peak located at 959 cm
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- 2022
16. Effect of the cavity disinfectant containing chitosan on dentin bonding strength after radiotherapy
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Derya Gursel Surmelioglu, Ayşenur Gungor Borsoken, Gorkem Kervancıoglu, and N Ezgi Yeniceri Hilaloglu
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Chitosan ,Infectious Diseases ,Dentin-Bonding Agents ,Tensile Strength ,Virology ,Dentin ,Chlorhexidine ,Humans ,Parasitology ,General Medicine ,Microbiology ,Disinfectants - Abstract
Introduction: This study aimed to compare the bond strength of teeth treated with radiotherapy with two cavity disinfectants (Chlorhexidine gluconate, a chitosan-containing agent). Methodology: Eighteen newly extracted, non-carious human third molar teeth were used. The teeth were randomly divided into two main groups, treated and/or non-treated with radiotherapy, then separated into three subgroups for disinfectant agent application (CHX, chitosan, control). A total dose of 70.2 Gy was given over 39 days using a linear radiation accelerator for radiotherapy. After applying the cavity disinfectant, the teeth were restored with composite resin to obtain the stick. All bond strength values from sticks were measured using a universal testing machine. The data were analyzed with ANOVA and Chi-square test at a p < 0.05. Results: Among all groups, Group CH, with no radiotherapy application and containing a chitosan-disinfectant agent, showed the highest bond strength (44.7 ± 8.2). In contrast, Group RC with radiotherapy and disinfectant-free showed the lowest bond strength value (29.1 ± 3.5). The highest bond strength values were obtained after applying the chitosan-containing agent in all groups. Conclusions: Radiotherapy application had a negative effect, while the use of disinfectant agents had a positive effect on the bond strength.
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- 2022
17. The comparative evaluation of the effects of quercetin, α‐tocopherol, and chlorhexidine dentin pretreatments on the durability of universal adhesives
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Marzieh Moradian, Maryam Saadat, Fatemeh Sohrabniya, and Mohammad Afifian
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Dentin-Bonding Agents ,alpha-Tocopherol ,Chlorhexidine ,Dentin ,Materials Testing ,Dental Bonding ,Dental Cements ,Quercetin ,General Dentistry - Abstract
The aim of this study was to evaluate and compare the effects of chlorhexidine, quercetin, and α-tocopherol on the shear bond strength of universal adhesives in the short (24h) and long term (6 months).Ninety-six extracted sound molars were collected and divided randomly into four groups: control (no treatment), 2% chlorhexidine, 10% α-tocopherol, and 1% quercetin. The solutions were prepared and applied to the teeth for 60 s, followed by application of All-Bond universal adhesive and composite build-up. Half of the specimens in each group (n = 12) were tested for shear bond strength (SBS) after 24 h of storage and the other half were kept in distilled water for 6 months and then tested for shear bond strength. The shear bond strength test was performed and the failure modes were determined using a stereomicroscope. The data were analyzed using two-way analysis of variance and Tukey's post hoc tests with p ˂ .05 as the significance level.The results of the two-way analysis of variance test showed that there was no significant difference in immediate SBS, and after 6 months, α-tocopherol had the lowest SBS in comparison to the control and CHX subgroups (p .05). The t-test showed that the shear bond strength in the α-tocopherol and quercetin groups was significantly decreased after 6 months.It can be concluded that the solutions used in this study had no adverse effect on immediate SBS. After 6 months, the CHX could preserve SBS in comparison to other groups.
- Published
- 2022
18. Dental Adhesives—Surface Modifications of Dentin Structure for Stable Bonding
- Author
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Bruna Marin, Fronza, Roberto Ruggiero, Braga, Milena, Cadenaro, Fronza, Bruna Marin, Braga, Roberto Ruggiero, and Cadenaro, Milena
- Subjects
Bond strength ,Dentin bonding systems ,Zymography ,Dentin bonding system ,Protease Inhibitor ,Collagen cross-linking agent ,Resin Cement ,Dental Bonding ,Collagen cross-linking agents ,MMP inhibitor ,Resin Cements ,Matrix metalloproteinase ,MMP inhibitors ,Peptide Hydrolase ,Matrix metalloproteinases ,Collagen ,Dentin ,Humans ,Peptide Hydrolases ,Protease Inhibitors ,Dentin-Bonding Agents ,General Dentistry ,Human - Abstract
The latest advancements in dentin bonding have focused on strategies to impair degradation mechanisms in order to extend the longevity of bonded interfaces. Protease inhibitors can reduce collagen degradation within the hybrid layer (HL). Collagen cross-linkers allow better adhesive infiltration and also inhibit proteases activity. Particles added to adhesive can promote mineral precipitation within the HL, reducing nanoleakage and micropermeability, besides possible antimicrobial and enzymatic inhibition effects. Most of these approaches are still experimental, and aspects of the adhesive under the clinician's control are still determinant for the long-term stability of adhesive restorations.
- Published
- 2022
19. Induction of Cartilage Regeneration by Nanoparticles Loaded with Dentin Matrix Extracted Proteins
- Author
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Sicong Mao, Sainan Wang, Yuting Niu, Jilin Wu, Peipei Jia, Jinxuan Zheng, and Yanmei Dong
- Subjects
Cartilage, Articular ,Tissue Engineering ,Biomedical Engineering ,Cell Differentiation ,Bioengineering ,Silicon Dioxide ,Biochemistry ,Rats ,Transforming Growth Factor beta1 ,Biomaterials ,Dentin ,Humans ,Animals ,Nanoparticles ,Chondrogenesis - Abstract
Due to the limited self-repair capacity of articular cartilage, tissue engineering has good application prospects for cartilage regeneration. Dentin contains several key growth factors involved in cartilage regeneration. However, it remains unknown whether dentin matrix extracted proteins (DMEP) can be utilized as a complex growth factor mixture to induce cartilage regeneration. In this work, we extracted DMEP from human dentin and improved the content and activity of chondrogenic-related growth factors in DMEP by alkaline conditioning. Afterward, mesoporous silica nanoparticles (MSNs) with particular physical and chemical properties were composed to selectively load and sustain the release of proteins in DMEP. MSN-DMEP promoted chondrogenic differentiation of rat bone marrow-derived mesenchymal stem cells with fewer growth factors than exogenously added transforming growth factor-β1 (TGF-β1). Therefore, MSN-DMEP may serve as a promising candidate for cartilage regeneration as an alternative to expensive synthetic growth factors. Impact statement Several growth factors embedded in dentin matrix could be involved in cartilage regeneration. This article reports that alkaline conditioning could improve the content and activity of chondrogenic-related growth factors in dentin matrix extracted proteins (DMEP). Mesoporous silica nanoparticles (MSNs) with particular physical and chemical properties performed well in loading and sustained releasing of proteins in DMEP.iIn vitro/iandiin vivo/istudies suggest that MSN-DMEP could be a promising candidate for cartilage regeneration as an alternative to expensive synthetic growth factors.
- Published
- 2022
20. Resin-based materials to control human dentin permeability under erosive conditions in vitro: A hydraulic conductance, confocal microscopy and FTIR study
- Author
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Victor, Mosquim, Rafaela Aparecida, Caracho, Giovanna Speranza, Zabeu, Luiza da Silveira, Condi, Gerson Aparecido, Foratori-Junior, Ana Flávia Sanches, Borges, and Linda, Wang
- Subjects
Microscopy, Confocal ,Dentin Desensitizing Agents ,Dentin Sensitivity ,Citric Acid ,EROSÃO DE DENTE ,Dentin Permeability ,Polymethacrylic Acids ,Glutaral ,Mechanics of Materials ,Smear Layer ,Dentin ,Spectroscopy, Fourier Transform Infrared ,Microscopy, Electron, Scanning ,Humans ,General Materials Science ,General Dentistry - Abstract
To characterize the behavior of three different polymeric agents before and after an erosive challenge on dentin permeability, to analyze their degradation in both conditions, and to analyze their degree of conversion (DC).The permeability of human dentin disks (1.0 ± 0.2 mm) was measured with smear layer, after its removal, after treatment (LpTreat) with Gluma Desensitizer, PRG Barrier Coat (PBC) or Icon infiltrant (n = 11/group) and after exposure to citric acid (LpEro) (6%, pH 2.1, 1 min). The specimens were analyzed under a Laser Scanning Confocal Microscope (n = 2/group) and the products' DC were calculated. Data were subjected to 2-way repeated measures ANOVA and post-hoc Bonferroni (permeability analysis), to paired t-test (for specimens treated with Icon) and to t-test (DC analysis) (α 0.05).Icon showed the lowest LpTreat and LpEro values, while PBC and Gluma did not differ from each other under these conditions. Icon and PBC showed LpEro similar to a dentin with smear layer. Under the Laser Scanning Confocal Microscope, more deposits were noticeable on dentin after treating with PBC. Gluma presented the deepest penetration in dentin. The DC of PBC was the highest.Icon caused the highest reduction on permeability values, followed by PBC and Gluma. PBC generated more deposits covering dentin and seemed to be more efficient after an erosive challenge. The association of a polymeric resin with inorganic ion-releasing fillers seem to be a great strategy to manage dentin hypersensitivity under erosive conditions.
- Published
- 2022
21. The differences of microleakage smart dentin replacement, glass ionomer cement and a flowable resin composite as orifice barrier in root canal treated
- Author
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Erma SOFİANİ and Emilisa SARİ
- Subjects
dentin ,flowable resin composite ,glass ionomer cement ,micro-leakage ,orifice barrier ,premolar ,root canal ,Health Care Sciences and Services ,General Earth and Planetary Sciences ,Sağlık Bilimleri ve Hizmetleri ,General Environmental Science - Abstract
This study was a laboratory experiment. The sample was 27 premolar teeth with one or two mandibular permanent teeth extracted consist of: a smart dentin replacement, glass ionomer cement, and a flowable resin composite. Teeth were prepared using a crown-down method and obturated using gutta percha and AH Plus. After placement of the orifice barrier with a thickness of 4 mm, the teeth were immersed in a 2% methylene blue solution at 37ºC for 24 hours. Teeth sectioned in the buccolingual direction and observation of microleakage using a stereomicroscope (M = 10×). The results showed that microleakage differences between a smart dentin replacement, glass ionomer cement, and a flowable resin composite. The smart dentin replacement has the smallest micro-leakage value of 1.70 but does not differ significantly with the flowable composite resin.
- Published
- 2022
22. Influence of atmospheric pressure changes on dentin bond strength of conventional, bulk-fill and single-shade resin composites
- Author
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Secil Ozkan, Ata, Canan, Akay, Emre, Mumcu, and Nazim, Ata
- Subjects
Atmospheric Pressure ,Dentin ,Materials Testing ,Public Health, Environmental and Occupational Health ,Humans ,Composite Resins - Abstract
Introduction: The purpose of this study was to investigate the dentin bond strength of composite resins in response to environmental pressure changes. Methods: Ninety extracted human molar teeth were used. A mould (3 mm x 4 mm) was adapted on dentin, resin composites (conventional [n = 30] and single-shade composites [Ohmnicroma] [n = 30]) were filled in two increments of 2 mm. The bulk-fill composites (n = 30) were filled with one 4 mm increment. The specimens were stored for 30 days in artificial saliva. The specimens were exposed to hyperbaric pressure (283.6 kPa; 2.8 atmospheres absolute [atm abs]) or hypobaric pressure (34.4 kPa; 0.34 atm abs) once daily for 30 days and the control group was stored at atmospheric pressure for 30 days. The bond strength was tested with a universal testing machine and the failures were examined with a stereomicroscope and scanning electron microscope. Statistical analyses were performed using analysis of variance with post hoc tests, and the Weibull analysis. Results: Regardless of environmental pressure changes, the bulk-fill composites showed the highest bond strength. There was no significant difference in bond strength between the hypobaric and atmospheric pressure (control) groups after 30 days in all resins. The hyperbaric group showed lower bond strength for bulk-fill composites than the control group. Conclusions: Dentists experienced in diving and aviation medicine should definitely take part in the initial and periodic medical examinations of divers and aircrew to give appropriate treatment. Bulk-fill composite resins can be preferred in divers and aircrew due to high bond strength values.
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- 2022
23. Bonding of universal adhesives to bur-cut dentin: Effect of double application and dentin moisture level
- Author
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Nooruldeen Ali SAEED, Antonin TICHY, and Yasushi SHIMADA
- Subjects
Adhesives ,Dentin-Bonding Agents ,Tensile Strength ,Dentin ,Materials Testing ,Dental Bonding ,Ceramics and Composites ,Dental Cements ,General Dentistry ,Resin Cements - Abstract
This study investigated how the double application of adhesives and dentin moisture level influence the microtensile bond strength (µTBS) of universal adhesives containing different hydrophilic monomers to bur-cut dentin. Four universal adhesives (Scotchbond Universal, Clearfil Universal Bond Quick, PrimeBond Universal, BeautiBond Universal) were applied to wet and dry bur-cut dentin either in one or two layers. The µTBS test was performed after 25,000 thermal cycles, and scanning electron microscopy was used for the analysis of failure mode and interfacial ultrastructure. Double application significantly improved µTBS on wet dentin (p0.001), but the effect was not significant on dry dentin (p0.050). Double application also enhanced the formation of resin tags and limited voids within the adhesive layer of BeautiBond Universal. Dentin moisture had a significant effect on µTBS only if the adhesives were applied in a single layer (p=0.007). Except for PrimeBond Universal, the tested universal adhesives performed better on dry dentin.
- Published
- 2022
24. Is whitening toothpaste safe for dental health?: RDA-PE method
- Author
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Jae-Heon, Kim, Soyeon, Kim, Van Mai, Truong, Joo Won, Lee, and Young-Seok, Park
- Subjects
Toothbrushing ,Calcium Pyrophosphate ,Ointments ,Bleaching Agents ,Tooth Abrasion ,Sodium Bicarbonate ,Dentin ,Materials Testing ,Ceramics and Composites ,Animals ,Cattle ,General Dentistry ,Toothpastes ,Hydrogen - Abstract
The relative dentin abrasivity-profilometry equivalent values were compared using non-contact profilometry with three subtypes of regular toothpaste and two subtypes of whitening toothpaste containing sodium bicarbonate and 35% hydrogen peroxide. Bovine dentin specimens were assigned to six groups: regular toothpaste (R): R1 (BAMBOO SALT GUM OINTMENT); R2 (MEDIAN TARTAR ORIGINAL); R3 (PERIOE Alpha), Reference slurry: RS (calcium pyrophosphate), whitening toothpaste (W): W1 (NET. WT); W2 (Vussen 28 WHITENING). Relative dentin abrasion-profilometry equivalent (RDA-PE) was determined by brushing 10,000 times (n=8). The pH of the toothpaste was measured (n=5) and the abrasive constituents of the toothpaste was analyzed by FE-SEM and EDS. The RDA-PE values ranged from 26 to 166, and the pH level ranges were 4.928-9.153. The RDA-PE value of the whitening toothpaste containing hydrogen peroxide was not high compared with that of the regular toothpaste. The RDA-PE values of whitening toothpaste could vary depending on the mechanism and ingredients of the whitening agents.
- Published
- 2022
25. Advances in scaffolds used for <scp>pulp–dentine</scp> complex tissue engineering: A narrative review
- Author
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Parisa Noohi, Mohammad J. Abdekhodaie, Mohammad H. Nekoofar, Kerstin M. Galler, and Paul M. H. Dummer
- Subjects
Tissue Engineering ,Tissue Scaffolds ,Polymers ,Dentin ,Regeneration ,General Dentistry ,Dental Pulp - Abstract
Pulp-dentine complex regeneration via tissue engineering is a developing treatment modality that aims to replace necrotic pulps with newly formed healthy tissue inside the root canal. Designing and fabricating an appropriate scaffold is a crucial step in such a treatment.The present study aimed to review recent advances in the design and fabrication of scaffolds for de novo regeneration of pulp-dentine complexes via tissue engineering approaches.A literature search was conducted using PubMed, Europe PMC, Scopus and Google Scholar databases. To highlight bioengineering techniques for de novo regeneration of pulp-dentine complexes, both in vitro and in vivo studies were included, and clinical studies were excluded.In the present review, four main classes of scaffolds used to engineer pulp-dentine complexes, including bioceramic-based scaffolds, synthetic polymer-based scaffolds, natural polymer-based scaffolds and composite scaffolds, are covered. Additionally, recent advances in the design, fabrication and application of such scaffolds are analysed along with their advantages and limitations. Finally, the importance of vascular network establishment in the success of pulp-dentine complex regeneration and strategies used to create scaffolds to address this challenge are discussed.In the tissue engineering platform, scaffolds provide structural support for cells to adhere and proliferate and also regulate cell differentiation and metabolism. Up to now, considerable progress has been achieved in the field of pulp-dentine complex tissue engineering, and a spectrum of scaffolds ranging from bioceramic-based to naturally derived scaffolds has been fabricated. However, in designing a suitable scaffold for engineering pulp-dentine complexes, a variety of characteristic parameters related to biological, structural, physical and chemical features should be considered.The variety of biomaterials and fabrication techniques provides a great opportunity to address some of the requirements for scaffolds in regenerative endodontics. However, more studies are required to develop an ideal scaffold for use in a clinical setting.
- Published
- 2022
26. A Review of Dentinogenesis Imperfecta and Primary Dentin Disorders in Dogs
- Author
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Jessica Mack Wilson, Cynthia Bell, Katherine Queck, and Kristin Scott
- Subjects
Dogs ,Odontoblasts ,General Veterinary ,Dentinogenesis Imperfecta ,Dentin ,Humans ,Animals ,Dog Diseases ,Esthetics, Dental ,Osteogenesis Imperfecta - Abstract
This review describes the clinical, radiographic and histologic characteristics of dentinogenesis imperfecta diagnosed in two unrelated young dogs without evidence of concurrent osteogenesis imperfecta. The dentition was noted to have generalized coronal discoloration ranging from grey-blue to golden brown. Clinical pulp exposure, coronal wear and fractures were observed as was radiographic evidence of endodontic disease, thin dentin walls or dystrophic obliteration of the pulp canal. The enamel was severely affected by attrition and abrasion despite histologically normal areas; loss was most likely due to poor adherence or support by the underlying abnormal dentin. Histologically, permanent and deciduous teeth examined showed thin, amorphous dentin without organized dentin tubules and odontoblasts had dysplastic cell morphology. Primary dentin disorders, including dentinogenesis imperfecta and dentin dysplasia, have been extensively studied and genetically characterized in humans but infrequently reported in dogs. Treatment in human patients is aimed at early recognition and multi-disciplinary intervention to restore and maintain normal occlusion, aesthetics, mastication and speech. Treatment in both humans and canine patients is discussed as is the documented genetic heritability of primary dentin disorders in humans.
- Published
- 2022
27. Comparison of the osteogenic effectiveness of an autogenous demineralised dentin matrix and Bio-Oss® in bone augmentation: a systematic review and meta-analysis
- Author
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Yanfei, Li, Wanhang, Zhou, Peiyi, Li, Qipei, Luo, Anqi, Li, and Xinchun, Zhang
- Subjects
Minerals ,Bone Transplantation ,Otorhinolaryngology ,Osteogenesis ,Bone Substitutes ,Dental Implantation, Endosseous ,Dentin ,Humans ,Surgery ,Oral Surgery - Abstract
This study aimed to evaluate the clinical efficacy and histological outcomes of autogenous demineralised dentin matrix (ADDM) as bone graft material compared with Bio-Oss® in bone augmentation for the treatment of patients with oral bone deficits. Eight databases (PubMed, EMBASE, Cochrane Library, Science Direct, Scopus, Web of Science, CNKI, and WFPD) were searched for randomised controlled trials (RCT) performed from the date of inception of each database to July 2021. The Cochrane Collaboration's risk assessment tool was used to conduct the methodological quality assessment. Stata 15.0 software was used to perform data analysis. Seven RCTs including 220 patients were considered eligible for this study. No significant difference was found in the percentage of new bone formation (NBF) and implant stability quotient (ISQ). Patients who received ADDM grafting showed a significantly lower sinus height (SH) and percentage of residual graft material (RGM) compared with Bio-Oss® grafting. ADDM is as effective as Bio-Oss® in bone augmentation for oral bone defects.
- Published
- 2022
28. The Effect of Primary Root Canal Treatment on Dentinal Tubule Penetration of Calcium Silicate–based Sealers during Endodontic Retreatment
- Author
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Ayşenur, Eğemen and Sema, Belli
- Subjects
Root Canal Filling Materials ,Epoxy Resins ,Silicates ,Dentin ,Retreatment ,Calcium Compounds ,Dental Pulp Cavity ,General Dentistry - Abstract
The aim of this study was to evaluate the effect of previous root canal treatment on dentinal tubule penetration of calcium silicate-based sealers during endodontic retreatment and to compare 2 different (commonly used and modified) tubule penetration depth measurement techniques.The crowns of mandibular premolar teeth were removed, and root canals were prepared (ProTaper Universal; Dentsply Sirona, York, PA) and obturated using AH Plus (AH; Dentsply DeTrey, Konstanz, Germany), MTA Fillapex (MTAF; Angelus, Londrina, Brazil), and WellRoot ST (WRST; Vericom, Gyeonggi-do, South Korea); 0.1% red Rhod-2 dye (Chemodex, St Gallen, Switzerland) was added to the sealers. After storage for 3 weeks (37°C, 100% humid), the following sealer combination subgroups were created (the sealer used during the primary root canal treatment/the sealer used during the retreatment, n = 8): AH/AH, AH/MTAF, and AH/WRST; MTAF/AH, MTAF/MTAF, and MTAF/WRST; and WRST/AH, WRST/MTAF, and WRST/WRST. Green Fluo-3 dye (0.1%) was added to the sealers during retreatment. Sealer penetration depths were measured at 8 points and averaged, and the ratio of the retreatment sealer to the initial sealer was recorded as the mean tubule penetration depth ratio in the first technique, whereas the percentage of the circumferential penetration area ratios of the retreatment and initial sealers was calculated in the second technique. Statistical analyses were performed using Kolmogorov-Smirnov, Kruskal-Wallis, and Mann-Whitney U tests.The mean penetration depth of AH and MTFA was found to be higher at the coronal third region in the control groups (P .05). Both methods showed a significant difference among the groups at the coronal and median regions (P .05). The AH/WRST, MTAH/AH, MTAF/MTAF, MTAF/WRSTS, and WRST/WRST groups showed deeper tubule penetration (first technique, P = .01). Both techniques showed that the penetration depth of the sealers was the same at the apical region (P .05), whereas a difference was found among the techniques at the coronal and median regions. The sealers used in the previous root canal treatment limited the penetration depth of the sealers used during the retreatment process (P .05). When MTAF was used as the initial sealer, the penetration depth of the second sealer did not change, whereas if WRST was the initial sealer, the penetration depth of the sealers at the median and apical regions is negatively affected during the retreatment process (P .05).Previous root canal treatment affects the penetration depth of calcium silicate-based sealers. The use of AH Plus and MTA Fillapex during the retreatment process showed an advantage. Both measurement techniques showed similar performances at the apical region, whereas the results varied at the coronal and midcoronal regions; therefore, a more stable sealer penetration measurement technique is still necessary.
- Published
- 2022
29. Effects of root canal enlargement on unprepared areas and coronal dentine thickness of three‐rooted maxillary first premolars with different root configurations: A stepwise <scp>micro‐CT</scp> study
- Author
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Marco A. Versiani, Kleber K. T. Carvalho, Jorge N. R. Martins, Antonio L. N. Custódio, Maurício A. A. Castro, Emílio Akaki, Yara T. C. S. Silva‐Sousa, and Manoel D. Sousa‐Neto
- Subjects
Dentin ,Maxilla ,Bicuspid ,X-Ray Microtomography ,Dental Pulp Cavity ,Tooth Root ,General Dentistry ,Root Canal Preparation - Abstract
To evaluate the effects of progressive root canal enlargements on the unprepared surface area and remaining dentine thickness of three-rooted maxillary first premolars with different root configurations.Thirty three-rooted maxillary first premolars with three root configurations (n = 10) were selected and scanned in a micro-CT device. The root canals were sequentially enlarged with rotary instruments sizes 30.02 (step 1), 30.04 (step 2) and 30.06 (step 3). After each step, a new scan was taken. Analysed parameters included morphometric measurements (length, volume and surface area), number of static voxels and minimal dentine thickness. Statistical analyses were performed with one-way anova post hoc Tukey tests and paired sample t-test at a significance level of 5%.No statistical differences were observed amongst groups regarding the morphometric parameters and static voxels (p .05). The minimal dentine thickness of the distobuccal root significantly changed depending on the root configuration (p .05), whilst no differences were observed in the other roots (p .05). A great variation in the position of the minimal dentine thickness was observed after preparation. Overall, mean percentage reduction in dentine thickness was higher in the buccal roots than in the palatal root (p .05). In the mesiobuccal and distobuccal root, the number of slices with minimal dentine thickness lower than 0.05 mm increases 2 to 3 times and 3 to 4 times, respectively, from steps 1 to 3.Instruments sizes 30.02 and 30.04 can be safely and effectively used to enlarge the buccal and palatal canals of three-rooted maxillary first premolars.
- Published
- 2022
30. A modified dentine infection model with fluorescent lipopolysaccharide and lipopolysaccharides sampling technique to compare XP‐Endo finisher and passive ultrasonic irrigation
- Author
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Rayyan A. Alfirdous, Theeb A. Alquiria, Rogerio C. Jacinto, and Frederico C. Martinho
- Subjects
Lipopolysaccharides ,Root Canal Irrigants ,Dentin ,Ultrasonics ,Dental Pulp Cavity ,Therapeutic Irrigation ,General Dentistry ,Root Canal Preparation - Abstract
The lipopolysaccharides-dentine-infection (LPS-dentine-infection) models and sampling techniques frequently used to evaluate LPS disinfection have limitations. In this study, a LPS-dentine-infection model was devised using fluorescent conjugate LPS. Secondly, a sampling technique using cryogenic grinding for intraradicular LPS analysis was evaluated. Thirdly, the effectiveness of the XP-endo Finisher (XP-EF) was compared with passive ultrasonic irrigation (PUI) in removing LPS from root canal system.Sixty-nine mandibular premolars were submitted to dentine pretreatment and inoculated with fluorescent LPS conjugate (Alexa Fluor® 594). Twenty-three teeth were analysed under confocal laser scanning microscopy (CLSM) to validate this modified LPS-dentine-infection model. Forty-six teeth were randomly divided into two experimental groups: XP-EF (n = 23) and PUI (n = 23). All teeth were instrumented with XP-endo shaper (XPS; FKG Dentaire) and 2.5% NaOCl. The root canals were sampled with paper points before (s1) and after (s2) instrumentation and after supplemental treatment (s3) with XP-EF and PUI. After s3, all roots were cryogenically ground for intraradicular LPS analysis (s4). Limulus amebocyte lysate assay was used for LPS quantification. The Friedman test was used for differences in LPS among four time-points (s1, s2, s3, and s4). Dunn's test was used for pairwise testing of time-points. The significance level was set at 5% (p .05).Fluorescent LPS conjugate was detected in 100% of the samples under CLSM with a penetration depth of approximately 400 μm into dentine. Chemo-mechanical preparation using XPS files significantly reduced LPS levels (p .05). Both the XPS and PUI improved the LPS disinfection (p .05), with no difference between them (p .05). LPS was recovered from all samples after cryogenic grinding. The residual amount of LPS detected using the cryogenically sampling technique at s4 was approximately three times greater than with the paper-point sampling technique at s3.This study established a modified LPS-dentine-infection model using fluorescent conjugate LPS, and validated a LPS sampling technique for using cryopulverization intraradicular LPS analysis. Moreover, both the XP-EF and PUI further improved LPS disinfection from the root canals, and the innovative XP-EF was as effective as PUI.
- Published
- 2022
31. Effect of nanosecond- and microsecond-pulse Er,Cr:YSGG laser ablation on dentin shear bond strength of universal adhesives
- Author
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Zhengdong, Xiong, Shanshan, Tu, Lingling, Jiang, Tingqing, Cheng, and Haihe, Jiang
- Subjects
Adhesives ,Dentin ,Dental Bonding ,Humans ,Water ,Surgery ,Laser Therapy ,Lasers, Solid-State ,Dermatology ,Shear Strength ,Composite Resins - Abstract
The study aimed to evaluate the bond strength of universal adhesives to dentin after Er,Cr:YSGG laser irradiation with nanosecond-domain and microsecond-domain pulses.Eighty extracted caries-free, sound human molars were divided into eight groups. The enamel was removed until the dentin occlusal flat dentin surface was exposed. Etch-and-rinse followed by adhesive was applied to group 1, and a self-etch adhesive was applied to group 2. Er,Cr:YSGG laser (3 mJ, 100 Hz, 100 ns), (3 mJ, 100 Hz, 150 μs), and (20 mJ, 100 Hz, 150 μs) were applied to groups 3-4, 5-6, and 7-8, respectively. The laser preparation was followed by self-etch adhesives or adhesives treatment. When the composite resin had been built up on the samples, the shear bond strength was tested, and the data were statistically analyzed using analysis of variance (ANOVA).Groups prepared with nanosecond-pulse laser showed significantly higher bond strength values than the microsecond-pulse laser groups and self-etch mode group, and the SEM photographs also showed more dentinal tubules and no damage in the ablation area. The shear bond strength of long pulse laser ablated was comparable to that of self-etching system when it was combined with a self-etch adhesive at low energy, but higher energy laser degraded shear bond strength.The pulse width of Er,Cr:YSGG laser affects the bond strength, nanosecond pulses of laser irradiation without water cooling can enhance bond strength, but microsecond pulses of laser cannot enhance bond strength.
- Published
- 2022
32. To etch or not to etch, Part II: On the hydrophobic-rich content and fatigue strength of universal adhesives
- Author
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Thiago Henrique Scarabello Stape, Tapio Viita-aho, Ana Sezinando, Roda Seseogullari-Dirihan, Eirini Eleftheriadi, Murat Mutluay, and Arzu Tezvergil-Mutluay
- Subjects
Acid Etching, Dental ,Surface Properties ,Mechanics of Materials ,Dentin-Bonding Agents ,Tensile Strength ,Dentin ,Materials Testing ,Dental Bonding ,Dental Cements ,General Materials Science ,General Dentistry ,Resin Cements - Abstract
To determine whether smear layer management, via conservative etching protocols, and the hydrophobic-rich content of hybrid layers would affect the fatigue strength of resin-dentin interfaces.Bar-shaped dentin beams obtained from sound third molars were wet-polished for 30 s. Dentin was etched with 32 % ortho-phosphoric acid for 3 or 15 s, 10 % meta-phosphoric acid for 15 s or by a prime-and-rinse application using a mild universal adhesive (Scotchbond Universal, 3M ESPE). Self-etch application served as control. Coating was performed with a solvent-free bisGMA-based resin. Composite buildups were made with a nanofilled composite. Resin-dentin beams with twin-bonded interfaces were sectioned and stored in deionized water for 24 h at 37 ℃ before 4-point flexural quasi-static monotonic testing (n = 16). Stress-life fatigue behavior was evaluated under cyclic loading (n = 35) by the staircase method at 4 Hz. The tension side of cyclic-loaded unfractured beams were evaluated under SEM, along with the micro-morphology of etched dentin surfaces. Monotonic data was analyzed by two-way ANOVA followed by the Tukey Test and cyclic-loaded data by Kruskal-Wallis on Ranks (α = 0.05).Etching protocols and higher hydrophobic-rich content produced significantly higher fatigue life distributions (p 0.05). Dentin demineralization was ranked as OPA 15 s MPA 15 s OPA 3 s P + R SE. Less aggressive etching and coating reduced crack formation at hybrid layers.Current oversimplification trends in resin-dentin bonding constitute a trade-off between hybridization quality and easier adhesive handling. Controlled dentin etching and increasing the hydrophobic-rich content of hybrid layers may be necessary to extend the longevity of mild universal adhesives.
- Published
- 2022
33. Exploiting Dentine Matrix Proteins in Cell-Free Approaches for Periradicular Tissue Engineering
- Author
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Josette Camilleri, Phillip Tomson, Nasir Zeeshan Bashir, Paul R. Cooper, and Satnam Virdee
- Subjects
Pathology ,medicine.medical_specialty ,Tissue Engineering ,Mesenchymal stem cell ,Biomedical Engineering ,Cell Differentiation ,Bioengineering ,Matrix (biology) ,Biology ,Biochemistry ,Regenerative medicine ,Root Canal Therapy ,Biomaterials ,Periradicular ,Tissue engineering ,Dentin ,medicine ,Animals ,Stem cell ,Wound healing ,Periapical Periodontitis - Abstract
The recent discovery of mesenchymal stem cells within periapical lesions (PL-MSC) has presented novel opportunities for managing periradicular diseases in adult teeth by way of enhancing tissue regeneration. This discovery coincides with the current paradigm shift toward biologically driven treatment strategies in endodontics, which have typically been reserved for non-vital immature permanent teeth. One such approach that shows promise is utilizing local endogenous non-collagenous dentine extracellular matrix components (dECM) to recruit and upregulate the intrinsic regenerative capacity of PL-MSCs
- Published
- 2022
34. Characterization of silver diamine fluoride cytotoxicity using microfluidic tooth-on-a-chip and gingival equivalents
- Author
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Shijia Hu, Giridharan Muniraj, Apurva Mishra, Kanglun Hong, Jing Li Lum, Catherine Hsu Ling Hong, Vinicius Rosa, and Gopu Sriram
- Subjects
Quaternary Ammonium Compounds ,Mechanics of Materials ,Lab-On-A-Chip Devices ,Dentin ,Microfluidics ,Gingiva ,Humans ,Silver Compounds ,Fluorides, Topical ,General Materials Science ,Dental Caries ,General Dentistry - Abstract
This study aims to characterize the cytotoxicity potential of silver diamine fluoride (SDF) on dental pulp stem cells (DPSC) and gingival equivalents.DPSC cultured on 96-well plates was exposed directly to SDF (0.0001-0.01%) and cell viability (ICDPSC exposed directly to SDF showed a dose-dependent reduction in cell viability (ICSDF penetrated the dentin (≤1 mm thick) inducing significant death of the pulp cells. SDF also disrupted gingival epithelial integrity resulting in mucosal corrosion.
- Published
- 2022
35. PEEK and fiberglass intra-radicular posts: influence of resin cement and mechanical cycling on push-out bond strength
- Author
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Lara Christie Monteiro, Vanessa Gallego Arias Pecorari, Isabela Guerra Gontijo, Giselle Maria Marchi, Débora Alves Nunes Leite Lima, and Flávio Henrique Baggio Aguiar
- Subjects
Dental Stress Analysis ,Dental Bonding ,Ketones ,Composite Resins ,Resin Cements ,Polyethylene Glycols ,Materials Testing ,Dentin ,Animals ,Cattle ,Glass ,Dental Pulp Cavity ,General Dentistry ,Post and Core Technique - Abstract
To evaluate the bond strength of four types of posts (pre-fabricated fiberglass post, fiberglass post anatomized with composite resin, milled fiberglass post, and milled polyetheretherketone (PEEK) post), and two types of resin cements (conventional and self-adhesive) by assessing immediate bond strength and post-mechanical aging at each root third.Bovine endodontically treated roots (16 groups, n = 8) were prepared and the posts were produced and luted; the specimens of aging groups were cycled (300,000 cycles under 50 N load at 1.2 Hz frequency); six slices of each root were obtained; push-out test was performed by using a universal testing machine (500 N load at 1 mm/min cross speed); fracture pattern was classified into five levels. The statistical analyses used were three-way ANOVA, Tukey's test (for bond strength), and Fisher's test (for fracture pattern) (α 0.05).Differences were found between the cements for posts (conventional: p 0.001; self-adhesive: p = 0.002), whereas no difference was found for root region (p = 0.941; p = 0.056, respectively); analysis of each root showed significant differences for cements (p 0.001), posts (p 0.001), and mechanical cycling (p = 0.001); in terms of double interaction, differences were found for posts and mechanical cycling (p = 0.005); no other interactions were observed (double or triple); the fracture pattern showed difference between the groups for both cements.Milled PEEK posts seem to be a good clinical option, but they require improvement of CAD-CAM technology and advances towards their adhesion.Milled posts are promising and can reduce clinical time for rehabilitation of extensively destroyed teeth.
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- 2022
36. Effectiveness of an actuator-driven pulsed water jet for removal of softened carious dentin
- Author
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Yuka, Yokoyama-Sato, Takashi, Nishioka, Yukihiro, Naganuma, Masatoshi, Takahashi, Atsuhiro, Nakagawa, Nobuhiro, Yoda, Keiichi, Sasaki, Nobuhiro, Takahashi, Teiji, Tominaga, and Masahiro, Iikubo
- Subjects
Hardness ,Dentin ,Ceramics and Composites ,Humans ,Water ,Dental Caries ,General Dentistry - Abstract
The aim of this study was to assess the utility of an actuator-driven pulsed water jet (ADPJ) as a hardness-dependent carious dentin removal device by using different outputs. Thirty-six plane surface dental caries samples were treated with the ADPJ at 150, 200, and 250 voltage (12 teeth each). The Knoop hardness number (KHN) and Ca/P mass ratio were measured at 70 μm from the deepest point of the removing groove. Furthermore, three other teeth samples were manually treated with the ADPJ at the three above mentioned voltages (one tooth each) for 1 min. The KHN and Ca/P mass ratio were measured at 70 μm from the surface of the residual dentin part. In both the KHN and Ca/P mass ratio, higher residual dentin depended on the applied voltage of ADPJ. The ADPJ enabled the removal of softened carious dentin in an applied voltage-dependent manner.
- Published
- 2022
37. Effects of concentration of sodium hypochlorite as an endodontic irrigant on the mechanical and structural properties of root dentine: A laboratory study
- Author
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Haiping Xu, Zhou Ye, Anqi Zhang, Fei Lin, Jing Fu, and Alex S. L. Fok
- Subjects
Tooth, Nonvital ,Root Canal Irrigants ,Sodium Hypochlorite ,Dentin ,Animals ,Humans ,Cattle ,Dental Pulp Cavity ,General Dentistry ,Edetic Acid ,Root Canal Preparation - Abstract
The use of high-concentration sodium hypochlorite (NaOCl) as an endodontic irrigant remains controversial because of its potential impact on the fracture strength of endodontically treated teeth. This study evaluated the effects of using different NaOCl concentrations, with 2-min-ethylenediaminetetraacetic acid (EDTA) as the final active irrigant, on the biomechanical and structural properties of root dentine.A new test method, which is more clinically relevant, was utilized to calculate the fracture strength of root dentine. Bovine incisors were used to obtain root dentine discs. The root canals were enlarged to mean diameter of 2.90 mm with a taper of 0.06. The resulting discs were divided into five groups (n = 20) and treated with different concentrations of NaOCl (5.25%, 2.5%, and 1.3%) for 30 min plus 17% EDTA for 2 min. The discs were then loaded to fracture by a steel rod with the same taper through the central hole. The fractured specimens were examined by scanning electron microscopy to evaluate changes in the dimensions of the remaining intertubular dentine and the tubular radius. Micro-hardness was also measured with a Knoop diamond indenter along a radius to determine the depth of dentine eroded by the irrigation. Results were analysed by one-way anova and the Tukey test. The level of significance was set at α = 0.05.The damage by NaOCl increased with its concentration. 5.25% NaOCl greatly reduced the fracture strength of root dentine from 172.10 ± 30.13 MPa to 114.58 ± 26.74 MPa. The corresponding reduction in micro-hardness at the root canal wall was 34.1%. The damages reached a depth of up to 400 μm (p .05). Structural changes involved the degradation of the intratubular wall leading to enlarged dentinal tubules and the loss of intertubular dentine. Changes in the microstructural parameters showed positive linear relationships with the fracture strength.With the adjunctive use of EDTA, NaOCl caused destruction to the intratubular surface near the root canal and, consequently, reduced the root dentine's mechanical strength. The higher the concentration of NaOCl, the greater the effect. Therefore, endodontists should avoid using overly high concentration of NaOCl for irrigation to prevent potential root fracture in endodontically treated teeth.
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- 2022
38. Effect of decontamination materials on bond strength of saliva-contaminated CAD/CAM resin block and dentin
- Author
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Kei, Takahashi, Tomohiro, Yoshiyama, Akihito, Yokoyama, Yasushi, Shimada, and Masahiro, Yoshiyama
- Subjects
Surface Properties ,Tensile Strength ,Dentin ,Materials Testing ,Dental Bonding ,Ceramics and Composites ,Computer-Aided Design ,Saliva ,Composite Resins ,General Dentistry ,Decontamination ,Resin Cements - Abstract
The aim of this study was to evaluate the effect of decontamination agents against saliva contamination on the bonding of computer-aided design/computer-aided manufacturing (CAD/CAM) resin block. Three commercially available decontamination agents were used in this study. The samples were subjected to microtensile bond strength test. A saliva protein staining test was performed by the pigment binding method to investigate the effect of removing saliva protein. All the decontamination agents could significantly restore the bond strength from the saliva contamination, and KATANA Cleaner showed no significant difference with the control. From the results of the saliva protein staining test, KATANA Cleaner showed higher removal effect of saliva protein for CAD/CAM resin block surface than the other materials due to the surface active effect of MDP salt. It was suggested that cleaners containing MDP salt were more effective in removing artificial saliva contamination than cleaners containing other ingredients for CAD/CAM resin blocks.
- Published
- 2022
39. Evaluation of Caries Removal Efficacy Using BRIX 3000 and Atraumatic Restorative Treatment in Primary Molars: A Clinical Comparative Study
- Author
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Nisha, Gupta, Nagalakshmi, Chowdhary, Vundela R, Reddy, Kiran, Nk, Ravigna, Peddi, and Mahesh, Kumar
- Subjects
Dental Caries Susceptibility ,Dental Atraumatic Restorative Treatment ,Dentin ,Humans ,Dental Caries ,Child ,Dental Care ,Dental Cavity Preparation ,Molar ,General Dentistry - Abstract
The aim of the present study was to compare and evaluate the efficacy of two minimally invasive methods in the removal of infected carious dentin in primary molars.Eighty primary molars with class I occlusal caries involving dentin were selected and allocated to either of the two groups using convenient sampling. Group A (The results indicated that BRIX 3000 was effective in the removal of caries from primary molars with a significant difference. While there was no statistically significant difference seen in pain perception on caries excavation using both the methods. Although, a mild discomfort was noted in Group B.Comparing the two minimally invasive techniques, the new chemomechanical solution (BRIX 3000) was an effective method for the removal of caries from primary molars. So, it can be considered an alternative to the conventional caries removal method in treating pediatric dental patients.BRIX 3000 gel is effective in the removal of caries in children who are anxious and apprehensive as it is a noninvasive method of treatment in the pediatric age group.
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- 2022
40. Hydrogel Swelling-Mediated Strain Induces Cell Alignment at Dentin Interfaces
- Author
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David Fraser, Tram Nguyen, Alexander Kotelsky, Whasil Lee, Mark Buckley, and Danielle S. W. Benoit
- Subjects
Biomaterials ,Tissue Engineering ,Periodontal Ligament ,Dentin ,Biomedical Engineering ,Hydrogels ,Polyethylene Glycols - Abstract
Cell and tissue alignment is a defining feature of periodontal tissues. Therefore, the development of scaffolds that can guide alignment of periodontal ligament cells (PDLCs) relative to tooth root (dentin) surfaces is highly relevant for periodontal tissue engineering. To control PDLC alignment adjacent to the dentin surface, poly(ethylene glycol) (PEG)-based hydrogels were explored as a highly tunable matrix for encapsulating cells and directing their activity. Specifically, a composite system consisting of dentin blocks, PEG hydrogels, and PDLCs was created to control PDLC alignment through hydrogel swelling. PDLCs in composites with minimal hydrogel swelling showed random alignment adjacent to dentin blocks. In direct contrast, the presence of hydrogel swelling resulted in PDLC alignment perpendicular to the dentin surface, with the degree and extension of alignment increasing as a function of swelling. Replicating this phenomenon with different molds, block materials, and cells, together with predictive modeling, indicated that PDLC alignment was primarily a biomechanical response to swelling-mediated strain. Altogether, this study describes a novel method for inducing cell alignment adjacent to stiff surfaces through applied strain and provides a model for the study and engineering of periodontal and other aligned tissues.
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- 2022
41. Influence of Irradiance and Exposure Times on the Mechanical and Adhesive Properties of Universal Adhesives with Dentin
- Author
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Afm, Cardenas, Fsf, Siqueira, A, Nuñez, R F, Nonato, Kgba, Cavalcanti, C J, Soares, A, Reis, and A D, Loguercio
- Subjects
Dental Leakage ,Adhesives ,Dentin-Bonding Agents ,Tensile Strength ,Dentin ,Materials Testing ,Dental Bonding ,Dental Cements ,Humans ,Composite Resins ,General Dentistry ,Resin Cements - Abstract
SUMMARY Objectives This study evaluated the influence of irradiance/exposure time on the Knoop hardness (KHN) and polymer cross-linking density (PCLD), as well as microtensile bond strength (μTBS), nanoleakage (NL), and in situ degree of conversion (DC) of universal adhesives. Methods and Materials Two universal adhesive systems, Clearfil Universal Bond Quick (CUQ) and Scotchbond Universal Adhesive (SBU), were light-cured using various irradiance/exposure times: 1400 mW/cm2 for 5 s (1400*5); 1400 mW/cm2 for 10 s (1400*10); 3200 mW/cm2 for 5 s (3200*5); and 3200 mW/cm2 for 10 s (3200*10). Adhesive disks from each group were used to measure PCLD by KHN. One hundred and twenty-eight human molars were randomly assigned to 16 groups according to the following variables: adhesive system vs adhesive strategies vs radiance/exposure times. After restoration, specimens were sectioned into resin-dentin sticks and tested for μTBS, NL, and DC. The data from PCLD (%), KHN, μTBS (MPa), NL (%), and DC (%) data were subjected to ANOVA and Tukey’s test (α=0.05). Results Significant reductions in KHN, μTBS, and DC (p=0.00001) values and an increase in NL and PCLD (p=0.00001) values were observed for 3200*10 when compared with other groups. Higher KHN, μTBS, and DC (p=0.000001) values were observed for 3200*5 in comparison with the other groups. The 1400*5 (7 J/cm2) and 1400*10 (14 J/cm2) groups showed intermediate values (p=0.000001). Conclusion Although similar results in terms of hardness, polymer cross-linking density and nanoleakage were observed when 5 seconds at 3200 mW/cm2 and 10 seconds at 1400 mW/cm2 groups were compared, the use of higher irradiance (3200 mW/cm2) for only 5 seconds showed better results in terms of bond strength and degree of conversion for both universal adhesives to dentin. The prolonged exposure time (10 seconds) at the higher irradiance (3200 mW/cm2) showed the worst results.
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- 2022
42. Influence of Dental Tissue Substructure and Dimension on the Fracture Strength of Lithium Disilicate and Zirconia Ceramics
- Author
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Mona Simon, Axel Schurig, Marc Schmitter, and Christian Höhne
- Subjects
Molar ,Universal testing machine ,Materials science ,Enamel paint ,030206 dentistry ,General Medicine ,stomatognathic diseases ,03 medical and health sciences ,0302 clinical medicine ,medicine.anatomical_structure ,stomatognathic system ,Flexural strength ,visual_art ,Dentin ,medicine ,visual_art.visual_art_medium ,Substructure ,Cubic zirconia ,Ceramic ,Oral Surgery ,Composite material - Abstract
PURPOSE To assess the influence of substructure and dimension on the fracture strength of ceramic discs made from both lithium disilicate ceramic and zirconia. MATERIALS AND METHODS A total of 128 intact maxillary third molars were collected, and standardized enamel and dentin discs were fabricated. Lithium disilicate ceramic (IPS e.max CAD, Ivoclar Vivadent; n = 64) and zirconia (Katana, Kuraray; n = 64) discs with 0.5-mm (n = 32 IPS and n = 32 Katana) and 1-mm (n = 32 IPS and n = 32 Katana) thickness were produced, and each group was divided into two subgroups (n = 16 each) that were luted to the enamel or dentin discs using Panavia V5 (Kuraray). Half of the specimens in each subgroup were aged (chewing simulation and thermocycling), and all specimens were loaded until fracture in a universal testing machine (Z010, Zwick/Roell). Differences between Katana and IPS with respect to enamel and dentin as substructure and the thickness of the ceramic were analyzed by use of a nonparametric test (Mann-Whitney U test). RESULTS In un-aged specimens, fracture loads were not significantly (P > .05) different between zirconia and IPS specimens for 1- or 0.5-mm thickness. However, fracture loads were significantly higher (P < .001) in specimens supported by enamel, independent of the ceramic material. In aged specimens, the fracture loads of all specimens were significantly (P < .01) higher when supported by enamel; however, in the 0.5-mm groups, zirconia achieved significantly higher breaking loads than IPS when luted to dentin. CONCLUSION When 0.5-mm ceramic discs were luted to dentin, zirconia outperformed IPS with respect to breaking loads.
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- 2022
43. Biomechanical Behavior and Life Span of Maxillary Molar According to the Access Preparation and Pericervical Dentin Preservation: Finite Element Analysis
- Author
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Nawar Naguib Nawar, Mohamed Kataia, Nada Omar, Engy M. Kataia, and Hyeon-Cheol Kim
- Subjects
Dentin ,Finite Element Analysis ,Longevity ,Stress, Mechanical ,Molar ,General Dentistry - Abstract
This study investigated the significance of pericervical dentin after coronal canal flaring on the biomechanical behavior and life span of a maxillary molar using finite element analysis (FEA).In addition to the intact tooth (IT) model, 4 experimental FE models were designed: conservative access cavity model (CON), and 3 models with different radicular preparations for the coronal 4 mL considering 3 instruments: ProTaper SX model (SX), OneFlare model (OF), and Gates-Glidden model (GG). Cyclic loading of 50 N was applied on the occlusal surface and number of cycles until failure (NCF) was compared with the IT model. Mathematical analysis was done to evaluate the stress distribution patterns and calculated maximum von Mises (VM) and maximum principal stresses.Access cavity preparation (CON) decreased NCF significantly when compared with the IT model (93.99%). The coronal preparation of the root canal did not have a significant effect even when the preparation was taken to the extreme (GG: 92.02%). VM analysis confirmed apical dispersion of stresses, with maximum value registered on the occlusal surface in the GG model (7.88 MPa), and minimum on the IT model (7.01 MPa). The furcation area showed higher maximum principal stresses, yet stress values remained minimal and distributed over larger surfaces with the progressive enlargement among models.Within the limitations of this study, coronal canal flaring affects tooth integrity minimally, and when loading conditions lie within normal functional ranges, tooth structure has the capacity to disperse increasing stresses over a wider surface area.
- Published
- 2022
44. Ultrastructural Analysis of Er:YAG Lased Bovine Dentin Contaminated by Cariogenic Bacteria
- Author
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Qing, Du, He, Li, Jie, Su, Li, Zhang, Sun, Zhang, Qian, Zhang, Jing, Ju, Xiao, Wang, and Yanting, Li
- Subjects
Bacteria ,Dentin ,Microscopy, Electron, Scanning ,Biomedical Engineering ,Animals ,Cattle ,Radiology, Nuclear Medicine and imaging ,Lasers, Solid-State - Published
- 2022
45. Interfacial Bond Strength and Morphology of Sound and Caries-affected Dentin Surfaces Bonded to Two Resin-modified Glass Ionomer Cements
- Author
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R M, Al-Hasan and L A, Al-Taee
- Subjects
Dental Caries Susceptibility ,Glass Ionomer Cements ,Dentin-Bonding Agents ,Dentin ,Materials Testing ,Dental Bonding ,Humans ,Dental Caries ,General Dentistry ,Aged ,Resin Cements - Abstract
SUMMARY Objective To evaluate the shear bond strength and interfacial morphology of sound and caries-affected dentin (CAD) bonded to two resin-modified glass ionomer cements (RMGICs) after 24 hours and two months of storage in simulated body fluid at 37°C. Methods and Materials Sixty-four permanent human mandibular first molars (32 sound and 32 with occlusal caries, following the International Caries Detection and Assessment System) were selected. Each prepared substrate (sound and CAD) was conditioned (10% polyacrylic acid) and bonded to Activa BioACTIVE Restorative (Activa) and Fuji II LC (F2LC) as per the manufacturers’ instructions. Shear bond strength (SBS) was performed after 24 hours and two months of storage. The interfacial surfaces were examined using a digital microscope and scanning electron microscope (SEM). Three-way ANOVA, Bonferroni post-hoc tests (α=0.05), and independent T-tests were used for multifactorial analysis. Results Activa exhibited reduced bond strength values to sound and CAD in comparison to F2LC after two time periods (p=0.01). There is a pronounced enhancement in SBS of F2LC when bonded to CAD (p=0.01) after storage, with no statistically significant effect on sound dentin (p=0.309). Activa showed stable SBS to sound and CAD immediately and post-aging (p>0.05). However, the evidence of mineral-like deposits under an SEM attached to the aged, debonded dentin surfaces, thereby obliterating the exposed dentinal tubules, might support the tissue repair potential of Activa. Conclusions The SBS of Activa was lower than F2LC when bonded to sound and CAD, but the bonding stability and sealing ability is advantageous in minimally invasive therapy, suggesting use with a resin composite coverage when used in stress-bearing areas.
- Published
- 2022
46. The effect of therapeutic radiation on dental enamel and dentin: A systematic review
- Author
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Laura Douchy, Rémy Gauthier, Hazem Abouelleil-Sayed, Pierre Colon, Brigitte Grosgogeat, and Julia Bosco
- Subjects
Head and Neck Neoplasms ,Mechanics of Materials ,Dentin ,Humans ,General Materials Science ,Dental Caries ,Dental Enamel ,Radiation Injuries ,Tooth ,General Dentistry - Abstract
The conventional radiotherapy protocol to treat head-and-neck cancer is usually followed by tooth-decay onset. Radiation impact on mineralized tooth structures is not well-understood. This systematic review aimed to collect the recorded effects of therapeutic radiation on tooth chemical, structural and mechanical properties, in relation with their means of investigation.Systematic search (January 01 2012 - September 30 2021) terms were "Radiotherapy", "Radiation effects", "Dental enamel", "Dentin", "Human" and "Radiotherapy" NOT "Laser".PubMed, DOSS and Embase databases were searched.Selected studies compared dental enamel, coronal and root dentin properties before and after in vitro or in vivo irradiation up to 80 Gy.The systematic search identified 353 different articles, with 28 satisfying inclusion criteria. Their reference lists provided two more. Twenty-two studies evaluated dental enamel evolution, nine assessed coronal dentin and eight concerned root dentin. Coronal and root dentin results indicate a major impact of the radiation on their organic matrix. Dental enamel's chemical properties are less modified. Enamel and root dentin's hardness are decreased by therapeutic radiation, but no consensus arises for coronal dentin.Our findings revealed some interesting information about enzymatic degradation mechanisms of dentin organic matrix and highlighted that dental hard-tissue characterization requires highly specific expertise in materials science. That scientific knowledge is necessary to design suitable protocols, adequately analyze the obtained data, and, thus, provide relevant conclusions.Better knowledge and understanding of the mechanisms involved in the degradation of enamel and dentin would enable development of new preventive and therapeutic methods for improved medical care of patients undergoing radiotherapy.
- Published
- 2022
47. Experimental pastes containing niobophosphate and 45S5 bioactive glasses for treatment of dentin hypersensitivity: dentin permeability and tubule obliteration
- Author
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Samantha Ariadne Alves de Freitas, Paulo Vitor Campos Ferreira, Edilausson Moreno Carvalho, Mayra Alejandra Nuñez Aldaz, Alessandro Dourado Loguercio, Renata Grazziotin-Soares, Ceci Nunes Carvalho, Adriana de Fátima Vasconcelos Pereira, and José Bauer
- Subjects
Dentin Permeability ,Dentin Desensitizing Agents ,Dentin ,Microscopy, Electron, Scanning ,Humans ,Dentin Sensitivity ,General Dentistry - Abstract
This study tested the ability of bioactive pastes containing niobophosphate and 45S5 glasses to reduce dentin permeability and to obliterate dentinal tubules, as a mean of reducing human dentin hypersensitivity.Experimental pastes with concentrations of 10, 20, and 30 wt% of two bioactive glasses (45S5 or niobophosphate [NbG]) were formulated. A paste without bioactive glass (placebo) and a commercial paste (Nano P, FGM) were used as controls. Forty dentin disc specimens were obtained from caries-free extracted third human molars and divided in 8 groups (n = 5). Percentage of permeability (%Lp) was assessed in a dental permeability machine considering hydraulic conductance, immediately after pastes application and at day 7, day 14, and day 21. The precipitates formed on the surface of the dentin discs (and dentinal tubules) were analyzed by SEM/EDS and micro-Raman spectra. Data of dentin permeability (%) 2-way repeated-measures (ANOVA) and Holm-Sidak post-tests (α = 0.05). Dentinal tubule obliteration was visually (and elemental) evaluated and descriptively reported.The experimental bioactive glass pastes containing NbG and 45S5, regardless of the concentration, reduced dentin permeability in comparison with pastes without bioactive glasses (P0.05). The formulated placebo and commercial paste did not reduce permeability over time (P0.05). SEM/EDS and micro-Raman analyses showed that both type of bioactive pastes (NbG or 45S5-based) presented mineral precipitates obliterating the dentinal tubules at day 21. NbG seems to offer a better initial effect than 45S5, while at 21 days there is no difference between both glasses.Experimental bioactive pastes containing NbG and 45S5 (at concentrations of 10%, 20%, or 30%) have potential to reduce dentin permeability (over time) in comparison with pastes without bioactive glasses; and this occurs on behalf of obliteration of dentinal tubules by microparticle and precipitate formation.Bioactive pastes containing NbG and 45S5 may benefit patients presenting dentin hypersensitivity, because these pastes can start acting fast after application and maintain their action up to 21 days.
- Published
- 2022
48. Efficacy of Rice Husk Nanosilica as A Caries Treatment (Dentin Hydroxyapatite and Antimicrobial Analysis)
- Author
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Sylva Dinie Alinda, Iffi Aprillia, and Endang Suprastiwi
- Subjects
History ,Polymers and Plastics ,Chemistry ,food and beverages ,Dental cavity ,Antimicrobial ,Husk ,Industrial and Manufacturing Engineering ,medicine.anatomical_structure ,stomatognathic system ,Antimicrobial effect ,Normal dentin ,Dentin ,medicine ,Food science ,Business and International Management ,Caries treatment ,General Dentistry - Abstract
Introduction: Rice husk nanosilica has a porous, amorphous structure with a surface containing silica (SiO2). Silica interacts with calcium ions to form hydroxyapatite and can induce the formation of reactive oxygen species (ROS), which harm microorganisms. Aims: To determine the effect of rice husk nanosilica on the increase in dentin hydroxyapatite and its antimicrobial effects against S. Mutans. Methods: Nine dental cavity samples were divided into 3 groups: group 1, normal dentin; group 2, demineralised dentin and group 3, demineralised dentin treated with rice husk nanosilica. The samples were analysed using XRD to evaluate the formation of dentin hydroxyapatite, and the results were statistically evaluated using the Kruskal-Wallis test. An antimicrobial test was conducted using an MTT assay to analyse the viability of S. mutans after exposure to 2% nanosilica rice husk, and the results were statistically evaluated using a t-test. Results: There was an increase in the amount of dentin hydroxyapatite after the application of rice husk nanosilica compared with the control group (normal dentin), and 2% rice husk nanosilica had an antimicrobial effect (p
- Published
- 2022
49. Clinical efficacy of autogenous dentin grafts with guided bone regeneration for horizontal ridge augmentation: a prospective observational study
- Author
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W. Wang, Y. Jiang, D. Wang, D. Mei, H. Xu, and B. Zhao
- Subjects
Bone Regeneration ,Bone Transplantation ,Treatment Outcome ,Otorhinolaryngology ,Dental Implantation, Endosseous ,Dentin ,Humans ,Surgery ,Alveolar Ridge Augmentation ,Oral Surgery - Abstract
The aim of this study was to evaluate the efficacy of autogenous dentin grafts with guided bone regeneration (GBR) for horizontal ridge augmentation. Nineteen patients with dentition and bone defects in whom tooth/teeth extraction was indicated were recruited. Autogenous teeth were prepared, fixed on the buccal sides of the defects, and covered with bone powder and resorbable membranes before implantation. The horizontal bone mass at 0 mm (W1), 3 mm (W2), and 6 mm (W3) from the alveolar crest was recorded using cone beam computed tomography, before, immediately after, and 6 months after dentin grafting. All adverse effects were recorded. The implant stability quotient (ISQ) was measured 6 months after implantation. Twenty-eight implants were placed 6 months after dentin grafting. At this time point, the bone mass was 4.72 ± 0.72 mm (W1), 7.35 ± 1.57 mm (W2), and 8.96 ± 2.38 mm (W3), which was significantly different from that before the surgery (P 0.05). The bone gain was 2.50 ± 0.72 mm (W1), 4.10 ± 1.42 mm (W2), and 4.56 ± 2.09 mm (W3). No soft tissue dehiscence or infection was observed. Overall, 26.3% of the patients experienced severe pain after dentin grafting. The ISQ was 78.31 ± 6.64 at 6 months after implantation. Autogenous tooth roots with GBR might be effective for horizontal ridge augmentation. This technique could be an alternative to augmentation using autogenous bone grafts.
- Published
- 2022
50. Nano-hydroxyapatite-incorporated polycaprolactone nanofibrous scaffold as a dentin tissue engineering-based strategy for vital pulp therapy
- Author
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Igor Paulino Mendes Soares, Caroline Anselmi, Fernanda Ali Kitagawa, Rafael Antonio de Oliveira Ribeiro, Maria Luísa Leite, Carlos Alberto de Souza Costa, Josimeri Hebling, Universidade Estadual Paulista (UNESP), and Faculty of Dentistry
- Subjects
Tissue Engineering ,Tissue Scaffolds ,Polyesters ,Nanofibers ,Calcium-phosphate ceramics ,Cell Differentiation ,Nanofiber ,Scaffold ,Durapatite ,Pulp capping agents ,Mechanics of Materials ,Dentin ,Cell-homing therapy ,Humans ,Tissue engineering ,General Materials Science ,Hydroxyapatites ,General Dentistry ,Dental Pulp ,Cell Proliferation - Abstract
Made available in DSpace on 2022-05-01T15:46:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Objectives: Targeting a tissue engineering-based vital pulp therapy (VPT), this study investigated the incorporation of nano-hydroxyapatite (nHA) into polycaprolactone (PCL) nanofibers, and the metabolism of human dental pulp cells (HDPCs) seeded on the scaffolds. Methods: PCL-based solutions (10% w/v) containing nHA (0 – control; 0.5; 1.0; or 2.0% w/v) were electrospun into nanofibrous scaffolds. The scaffolds were characterized for morphology and composition (MEV/EDS), solubility, the release of calcium/phosphate (C/P), and modulation of medium pH. Then, HDPCs were seeded on the scaffolds and evaluated for cell viability (alamarBlue and live/dead), adhesion and spreading (F-actin), total protein (TP; Lowry), alkaline phosphatase activity (ALP; thymolphthalein assay), expression of odontogenic genes (RT-qPCR), and formation of a mineralized matrix (Alizarin Red). Data were analyzed with ANOVA and post-hocs (α = 5%). Results: Higher nHA concentrations roughened fiber surfaces, whereas PCL+ 2%nHA increased the interfibrillar spaces. PCL+ 1%nHA or PCL+ 2%nHA significantly released more C/P but the medium pH was maintained below 8.0. HDPCs viability was not affected by nHA, while cell adhesion/spreading was favored, especially for PCL+ 2%nHA. Higher protein content and ALP activity were seen for scaffolds incorporated with nHA, after 21 days. PCL+ 1%nHA and PCL+ 2%nHA upregulated the expression of DSPP and DMP1 in 14 days, and COL1A1, ALPL, and DMP1 in 21 days. The formation of a mineralized matrix was nHA concentration-dependent, and it was about 9 × higher for PCL+ 2%nHA. Significance: nHA-incorporated PCL nanofibrous scaffolds are cytocompatible and can stimulate the adhesion and odontogenic potential of HDPCs. PCL+ 2%nHA formulation is a bioactive tissue engineering-based cell-homing strategy for VPT. Department of Dental Materials and Prosthodontics São Paulo State University (UNESP) School of Dentistry Department of Genetics Morphology Orthodontics and Pediatric Dentistry São Paulo State University (UNESP) School of Dentistry Department of Oral Health Sciences The University of British Columbia Faculty of Dentistry Department of Physiology and Pathology São Paulo State University (UNESP) School of Dentistry Department of Dental Materials and Prosthodontics São Paulo State University (UNESP) School of Dentistry Department of Genetics Morphology Orthodontics and Pediatric Dentistry São Paulo State University (UNESP) School of Dentistry Department of Physiology and Pathology São Paulo State University (UNESP) School of Dentistry FAPESP: 2019/07400–0 FAPESP: 2019/16473–1
- Published
- 2022
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