1. 1α,25-dihydroxyvitamin D3triggered vitamin D receptor and farnesoid X receptor-like effects in rat intestine and liverin vivo
- Author
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Shanjun Liu, Geny M. M. Groothuis, Han-Joo Maeng, Ansar A. Khan, K. Sandy Pang, Edwin C. Y. Chow, Nanomedicine & Drug Targeting, Groningen Research Institute of Pharmacy, and Biopharmaceuticals, Discovery, Design and Delivery (BDDD)
- Subjects
Male ,Pharmaceutical Science ,enzymes, bile acids ,UP-REGULATION ,Calcitriol receptor ,1 alpha ,Western blotting ,Rats, Sprague-Dawley ,fibroblast growth factor 15 or FGF15 ,Pharmacology (medical) ,Intestinal Mucosa ,Vitamin D ,Asbt ,Cyp7a1 ,TISSUE DISTRIBUTION ,GENE-EXPRESSION ,Bile acid ,Mrp4 ,INDUCTION ,Multidrug resistance-associated protein 2 ,Mrp2 ,Asbt, Bsep, P-gp ,Mrp3 ,General Medicine ,Intestines ,medicine.anatomical_structure ,Multidrug Resistance-Associated Proteins ,BILE-ACID TRANSPORTER ,medicine.medical_specialty ,medicine.drug_class ,enzymes ,Ileum ,transporters ,Biology ,liver ,Cholesterol 7 alpha-hydroxylase ,the vitamin D receptor or VDR, farnesoid X receptor or FXR ,D ANALOGS ,Bsep ,Internal medicine ,medicine ,Animals ,ATP Binding Cassette Transporter, Subfamily B, Member 1 ,RNA, Messenger ,intestine ,1,25-DIHYDROXYVITAMIN D-3 ,bile acids ,Pharmacology ,short heterodimer partner or SHP, fibroblast growth factor 15 or FGF15, Western blotting, qRT-PCR, liver ,the vitamin D receptor or VDR ,FGF15 ,1 alpha,25-dihydroxyvitamin D-3 ,farnesoid X receptor or FXR ,qRT-PCR ,Cyp3a ,25-dihydroxyvitamin D-3 ,Small intestine ,Rats ,ALPHA ,Endocrinology ,nuclear receptors or NRs ,intestine, transporters ,CELLS ,Receptors, Calcitriol ,P-gp ,Farnesoid X receptor ,NUCLEAR RECEPTORS ,short heterodimer partner or SHP - Abstract
1 alpha,25-Dihydroxyvitamin D-3 (1,25(OH)(2)D-3), a natural ligand of the vitamin D receptor (VDR), was found to increase the rat ileal Asbt and bile acid absorption. The effects of VDR, whose expression is low in liver, on hepatic transporters and enzymes are Unknown Protein and mRNA levels of target genes in the small intestine, colon and liver after intraperitoneal closing of 1,25(OH)(2)D-3 (0-2.56 nmol/kg/day for 4 days) to the rat were determined by Western blotting and qPCR, respectively The 1,25(OH)(2)D-3 treatment increased total Cyp3a protein and Cyp3a1 mRNA expressions in the proximal small intestine, and the short heterodimer partner (SHP), the fibroblast growth factor,15 (FGF15), organic solute transporter (Ost alpha and Ost beta) mRNA and Asbt protein expressions in the ileum About 50% higher portal bile acid concentration (65.1 +/- 14.9 vs 419 +/- 7.8 mu M, p 50% reduction in the Cyp7a1 protein level (p
- Published
- 2009
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