29 results on '"D'ONOFRIO, NUNZIA"'
Search Results
2. Additional file 1 of Glycated ACE2 reduces anti-remodeling effects of renin-angiotensin system inhibition in human diabetic hearts
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Marfella, Raffaele, D’Onofrio, Nunzia, Mansueto, Gelsomina, Grimaldi, Vincenzo, Trotta, Maria Consiglia, Sardu, Celestino, Sasso, Ferdinando Carlo, Scisciola, Lucia, Amarelli, Cristiano, Esposito, Salvatore, D’Amico, Michele, Golino, Paolo, De Feo, Marisa, Signoriello, Giuseppe, Paolisso, Pasquale, Gallinoro, Emanuele, Vanderheyden, Marc, Maiello, Ciro, Balestrieri, Maria Luisa, Barbato, Emanuele, Napoli, Claudio, and Paolisso, Giuseppe
- Abstract
Additional file 1: Figure S1. Western blot analysis of GlycACE2. Representative immunoblotting of recombinant hACE2 protein (ab151852, Abcam) after in vitro long-term exposure to glucose 120 mM was separated on SDS-PAGE by using 7% gels in reducing and non-reducing conditions and then transferred on nitrocellulose membrane. Membrane was incubated with specific primary antibody against GlycACE2 (1:1000) (#4355, Cell Signaling Technology). Molecular weight indicators are displayed at the center. Figure S2. Ejection fraction, TAPSE, and the E/e′ at week 1 (Basal) and week 48 (follow-up) in nondiabetic and diabetic patients treated with ACE-inhibitors (ACE-I) and angiotensin receptor blocker (ARB). Data are mean ± SD. *P < 0.05 vs non-diabetics, §P < 0.05 vs basal values. Figure S3. GlycACE2 at week 1 (Basal) and week 48 (Follow-up) in the diabetic patients with good glycemic control (HbA1c < 7%) and diabetic patients with poor glycemic control (HbA1c ≥ 7%) treated with ACE-inhibitors (ACE-I) and angiotensin receptor blocker (ARB). Data are mean ± SD. *P < 0.05 vs non-diabetics, §P < 0.05 vs basal values. Figure S4. A Angiotensin-1–9 (Ang-1–9), Ang 1–7, Mas receptor (MasR), Nuclear factor of activated T-cells (NFAT), in explanted hearts (HTX) at week 1 (Basal) and week 48 (Follow-up) from HTX, in nondiabetic and diabetic patients, treated with ACE-inhibitors (ACE-I) and angiotensin receptor blocker (ARB). B Ang-1–9, Ang 1–7, MasR, and NFAT, in explanted hearts (HTX) at week 1 (Basal) and week 48 (Follow-up) from HTX,in the diabetic patients with good glycemic control (HbA1c < 7%) and diabetic patients with poor glycemic control (HbA1c ≥ 7%) treated with ACE-inhibitors (ACE-I) and angiotensin receptor blocker (ARB). Data are mean ± SD. *P < 0.05 vs non-diabetics, §P < 0.05 vs basal values. Figure S5. Fibrosis percentage in explanted hearts (HTX), at week 1 (Basal) and week 48 (follow-up) from HTX, in the diabetic patients with good glycemic control (HbA1c < 7%) and diabetic patients with poor glycemic control (HbA1c ≥ 7%) treated with ACE-inhibitors (ACE-I) and angiotensin receptor blocker (ARB). Data are mean ± SD. *P < 0.05 vs non-diabetics, §P < 0.05 vs basal values. Table S1. Multivariate linear regression analysis with GlycACE2 as dependent variable.
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- 2022
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3. Antioxidant and Anti-Inflammatory Activities of Buffalo Milk δ-Valerobetaine
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D'Onofrio N, Balestrieri A, Neglia G, Monaco A, Tatullo M, CASALE, Rosario, Limone A, Balestrieri ML, Campanile G., D'ONOFRIO, NUNZIA, D'Onofrio, N, Balestrieri, A, Neglia, G, Monaco, A, Tatullo, M, Casale, Rosario, Limone, A, Balestrieri, Ml, Campanile, G., D'Onofrio, Nunzia, D'Onofrio, N., Balestrieri, A., Neglia, G., Monaco, A., Tatullo, M., Casale, R., Limone, A., and Balestrieri, M. L.
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buffalo milk ,0106 biological sciences ,medicine.medical_specialty ,Antioxidant ,Buffaloes ,medicine.drug_class ,medicine.medical_treatment ,Metabolite ,Anti-Inflammatory Agents ,medicine.disease_cause ,01 natural sciences ,Antioxidants ,Anti-inflammatory ,Lipid peroxidation ,Rumen ,chemistry.chemical_compound ,SIRT1 ,Sirtuin 1 ,Internal medicine ,SIRT6 ,medicine ,Animals ,chemistry.chemical_classification ,reactive oxygen specie ,Endothelial Cell ,Reactive oxygen species ,Animal ,Chemistry ,010401 analytical chemistry ,NF-kappa B ,Endothelial Cells ,δ-valerobetaine ,Oxidative Stre ,General Chemistry ,Buffaloe ,0104 chemical sciences ,Betaine ,Oxidative Stress ,Anti-Inflammatory Agent ,Milk ,Cytokine ,Endocrinology ,Reactive Oxygen Species ,General Agricultural and Biological Sciences ,hyperglycaemia ,Oxidative stress ,010606 plant biology & botany - Abstract
delta-Valerobetaine (delta VB), a constitutive metabolite of ruminant milk, is produced in the rumen from free dietary N-epsilon- trimethyllysine occurring ubiquitously in vegetable kingdom. The biological role of delta VB is poorly known. Here, the anti-oxidant and anti-inflammatory potential of buffalo milk delta VB was tested in vitro during high-glucose (HG)-induced endothelial damage. Results indicated that delta VB (0.5 mM) ameliorated the HG cytotoxicity (0.57 +/- 0.02 vs 0.41 +/- 0.018 O.D. (P < 0.01). Interestingly, buffalo milk extracts enriched with delta VB showed improved significant efficacy in decreasing reactive oxygen species, lipid peroxidation, and cytokine release during HG treatment compared to milk extracts alone (P < 0.05). It is noteworthy that delta VB reduced the HG-activated inflammatory signal by modulating SIRT1 (0.96 +/- 0.05 vs 0.85 +/- 0.04 AU), SIRT6 (0.82 +/- 0.04 vs 0.61 +/- 0.03 AU), and NF-kappa B (0.85 +/- 0.03 vs 1.23 +/- 0.03 AU) (P < 0.05). On the whole, our data show the first evidence of delta VB efficacy in reducing endothelial oxidative stress and inflammation, suggesting a potential role of this betaine as a novel dietary compound with health-promoting properties.
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- 2019
4. Additional file 2 of Glycated ACE2 receptor in diabetes: open door for SARS-COV-2 entry in cardiomyocyte
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D’Onofrio, Nunzia, Scisciola, Lucia, Sardu, Celestino, Trotta, Maria Consiglia, De Feo, Marisa, Maiello, Ciro, Mascolo, Pasquale, De Micco, Francesco, Turriziani, Fabrizio, Municinò, Emilia, Monetti, Pasquale, Lombardi, Antonio, Napolitano, Maria Gaetana, Marino, Federica Zito, Ronchi, Andrea, Grimaldi, Vincenzo, Hermenean, Anca, Rizzo, Maria Rosaria, Barbieri, Michelangela, Franco, Renato, Campobasso, Carlo Pietro, Napoli, Claudio, Municinò, Maurizio, Paolisso, Giuseppe, Balestrieri, Maria Luisa, and Marfella, Raffaele
- Abstract
Additional file 2: Figure S2. Mass spectra of the triply charged hACE2 tryptic peptide 342-357 in the glycated (panel A) and non-glycated (panel B) form. hACE2 is for human Angiotensin Converting enzyme 2 type.
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- 2021
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5. Ergothioneine oxidation in the protection against high-glucose induced endothelial senescence: Involvement of SIRT1 and SIRT6
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D'Onofrio, Nunzia, SERVILLO, Luigi, GIOVANE, Alfonso, CASALE, Rosario, Vitiello, Milena, MARFELLA, Raffaele, PAOLISSO, Giuseppe, BALESTRIERI, Maria Luisa, D'ONOFRIO, NUNZIA, D'Onofrio, Nunzia, Servillo, Luigi, Giovane, Alfonso, Casale, Rosario, Vitiello, Milena, Marfella, Raffaele, Paolisso, Giuseppe, and Balestrieri, Maria Luisa
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0301 basic medicine ,SIRT6 ,Senescence ,Small interfering RNA ,Src Homology 2 Domain-Containing, Transforming Protein 1 ,Cell ,Biochemistry ,Antioxidants ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Endothelial cell ,Sirtuin 1 ,Downregulation and upregulation ,Physiology (medical) ,medicine ,Hyperglycaemia ,Sirtuin ,Humans ,Sirtuins ,Histidine ,Cellular Senescence ,biology ,Endothelial Cells ,Ergothioneine ,ROS ,Cell biology ,Betaine ,Oxidative Stress ,Glucose ,030104 developmental biology ,medicine.anatomical_structure ,Diabetes Mellitus, Type 2 ,Gene Expression Regulation ,chemistry ,Hyperglycemia ,030220 oncology & carcinogenesis ,biology.protein ,Cell senescence ,Antioxidant - Abstract
Ergothioneine (Egt), the betaine of 2-mercapto-L-histidine, is a dietary antioxidant protecting against many diseases, including cardiovascular disease (CVD), through a redox mechanism different from alkylthiols. Here, experiments were designed to evaluate the mechanisms underlying the beneficial effect of Egt against hyperglycaemia-induced senescence in endothelial cells. To this end, cells were incubated with increasing concentrations of Egt (0.01-1.00mM) for 12h followed by incubation for 48h with high-glucose (25mM). Cell evaluation indicated that viability was not affected by mM concentrations of Egt and that the high-glucose cytotoxicity was prevented with the highest efficacy at 0.5mM Egt. The cytoprotective effect of Egt was paralleled by reduced ROS production, cell senescence, and, interestingly, the formation of hercynine (EH), a betaine we recently found to be produced during the Egt oxidation pathway. Notably, the Egt beneficial effect was exerted through the upregulation of sirtuin 1 (SIRT1) and sirtuin 6 (SIRT6) expression and the downregulation of p66Shc and NF-κB. SIRT1 activity inhibition and SIRT6 gene silencing by small interfering RNA abolished the protective effect of Egt against the high-glucose-induced endothelial senescence. These data provide the first evidence of the Egt ability to interfere with endothelial senescence linked to hyperglycaemia through the regulation of SIRT1 and SIRT6 signaling, thus further strengthening the already assessed role of these two histone deacetylases in type 2 diabetes.
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- 2016
6. Ergothioneine Antioxidant Function: From Chemistry to Cardiovascular Therapeutic Potential
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SERVILLO, Luigi, D'Onofrio, N, BALESTRIERI, Maria Luisa, D'ONOFRIO, NUNZIA, Servillo, Luigi, D'Onofrio, N, Balestrieri, Maria Luisa, and D'Onofrio, Nunzia
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0301 basic medicine ,Antioxidant ,medicine.medical_treatment ,Antioxidants ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Betaine ,medicine ,Animals ,Humans ,Pharmacology ,Organic cation transport proteins ,biology ,Ergothioneine ,Transporter ,Glutathione ,Oxidative Stress ,030104 developmental biology ,chemistry ,Mechanism of action ,Biochemistry ,Cardiovascular Diseases ,030220 oncology & carcinogenesis ,biology.protein ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,Cysteine - Abstract
Ergothioneine (ESH), the betaine of 2-mercapto-L-histidine, is a water-soluble naturally occurring amino acid with antioxidant properties. ESH accumulates in several human and animal tissues up to millimolar concentration through its high affinity transporter, namely the organic cation transporter 1 (OCTN1). ESH, first isolated from the ergot fungus (Claviceps purpurea), is synthesized only by Actinomycetales and non-yeast-like fungi. Plants absorb ESH via symbiotic associations between their roots and soil fungi, whereas mammals acquire it solely from dietary sources. Numerous evidence demonstrated the antioxidant and cytoprotective effects of ESH, including protection against cardiovascular diseases, chronic inflammatory conditions, ultraviolet radiation damages, and neuronal injuries. Although more than a century after its discovery has gone by, our understanding on the in vivo ESH mechanism is limited and this compound still intrigues researchers. However, recent evidence about differences in chemical redox behavior between ESH and alkylthiols, such as cysteine and glutathione, has opened new perspectives on the role of ESH during oxidative damage. In this short review, we discuss the role of ESH in the complex machinery of the cellular antioxidant defense focusing on the current knowledge on its chemical mechanism of action in the protection against cardiovascular disease.
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- 2017
7. Ophthalmic acid is a marker of oxidative stress in plants as in animals
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Servillo, Luigi, Castaldo, Domenico, Giovane, Alfonso, Casale, Rosario, D'Onofrio, Nunzia, Cautela, Domenico, Balestrieri, Maria Luisa, D'ONOFRIO, NUNZIA, Servillo, Luigi, Castaldo, Domenico, Giovane, Alfonso, Casale, Rosario, D'Onofrio, Nunzia, Cautela, Domenico, and Balestrieri, Maria Luisa
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0106 biological sciences ,0301 basic medicine ,Ophthalmic acid ,Avena ,Biophysics ,Tripeptide ,Norophthalmic acid ,medicine.disease_cause ,01 natural sciences ,Biochemistry ,03 medical and health sciences ,chemistry.chemical_compound ,Paraquat ,Biosynthesis ,Tandem Mass Spectrometry ,medicine ,Animals ,Molecular Biology ,Chromatography, High Pressure Liquid ,Triticum ,Secale ,food and beverages ,Hordeum ,Glutathione ,Plants ,Plant Leaves ,Metabolic pathway ,Oxidative Stress ,030104 developmental biology ,chemistry ,Oxidative stre ,Oligopeptides ,Biogenesis ,Oxidative stress ,Biomarkers ,010606 plant biology & botany - Abstract
Background Ophthalmic acid (OPH), γ-glutamyl-L-2-aminobutyryl-glycine, a tripeptide analogue of glutathione (GSH), has recently captured considerable attention as a biomarker of oxidative stress in animals. The OPH and GSH biosynthesis, as well as some biochemical behaviors, are very similar. Here, we sought to investigate the presence of OPH in plants and its possible relationship with GSH, known to possess multiple functions in the plant development, growth and response to environmental changes. Methods HPLC-ESI-MS/MS analysis was used to examine the occurrence of OPH in leaves from various plant species, and flours from several plant seeds. Different types of oxidative stress, i.e., water, dark, paraquat, and cadmium stress, were induced in rye, barley, oat, and winter wheat leaves to evaluate the effects on the levels of OPH and its metabolic precursors. Results OPH and its dipeptide precursor, γ-glutamyl-2-aminobutyric acid, were found to occur in phylogenetically distant plants. Interestingly, the levels of OPH were tightly associated with the oxidative stress tested. Levels of OPH precursors, γ-glutamyl-2-aminobutyric acid and 2-aminobutyric acid, the latter efficiently formed in plants via biosynthetic pathways absent in the animal kingdom, were also found to increase during oxidative stress. Conclusions OPH occurs in plants and its levels are tightly associated with oxidative stress. General significance OPH behaves as an oxidative stress marker and its biogenesis might occur through a biochemical pathway common to many living organisms.
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- 2017
8. Serotonin 5-O-β-Glucoside and Its N-Methylated Forms in Citrus Genus Plants
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SERVILLO, Luigi, GIOVANE, Alfonso, CASALE, Rosario, D'Onofrio, Nunzia, FERRARI, Giovanna, Cautela, Domenico, BALESTRIERI, Maria Luisa, CASTALDO, Domenico, D'ONOFRIO, NUNZIA, Servillo, Luigi, Giovane, Alfonso, Casale, Rosario, D'Onofrio, Nunzia, Ferrari, Giovanna, Cautela, Domenico, Balestrieri, Maria Luisa, and Castaldo, Domenico
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Tryptamine ,Citrus ,Serotonin ,Tryptamine derivative ,Glucoside ,Citru ,Bufotenidine 5-O-β-glucoside ,Methylation ,Serotonin derivative ,Plant Extract ,chemistry.chemical_compound ,Hydrolysis ,Citrus plants ,Biotic stress ,Glucosides ,Tryptamine derivatives ,Serotonin 5-O-β-glucoside ,Plant defense against herbivory ,Citrus plant ,Indole test ,Serotonin derivatives ,Molecular Structure ,Plant Extracts ,Chemistry ,Chemistry (all) ,Bufotenidine ,food and beverages ,General Chemistry ,Bufotenine 5-O-β-glucoside ,N-methylserotonin 5-O-β-glucoside ,Agricultural and Biological Sciences (all) ,Biotic stre ,Biochemistry ,General Agricultural and Biological Sciences - Abstract
Citrus genus is characterized by a specific presence of indole metabolites deriving from the N-methylation of tryptamine and its hydroxylated form, 5-hydroxytryptamine (serotonin), which are likely involved in plant defense mechanisms. In this study, we identified for the first time the occurrence in Citrus plants of serotonin 5-O-β-glucoside and all its N-methylated derivatives, that is, N-methylserotonin 5-O-β-glucoside, N,N-dimethylserotonin (bufotenine) 5-O-β-glucoside, and N,N,N-trimethylserotonin (bufotenidine) 5-O-β-glucoside. The identification of the glucosylated compounds was based on mass spectrometric studies, hydrolysis by glucosidase, and in some cases, comparison to authentic compounds. Beside leaves, the distribution of the glucosylated forms and their aglycones in some Citrus species was evaluated in flavedo, albedo, juice, and seeds. The simultaneous presence of serotonin and its N-methylated derivatives, together with the corresponding glucosylated forms, is consistent with the occurrence of a metabolic pathway, specific for Citrus, aimed at potentiating the defensive response to biotic stress through the optimization of the production and use of the most toxic of such metabolites.
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- 2015
9. Sirtuin 6 Expression and Inflammatory Activity in Diabetic Atherosclerotic Plaques: Effects of Incretin Treatment
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BALESTRIERI, Maria Luisa, RIZZO, Maria Rosaria, BARBIERI, Michelangela, Paolisso P, D'Onofrio N, GIOVANE, Alfonso, Siniscalchi M, Minicucci F, Sardu C, D'Andrea D, Mauro C, FERRARACCIO, Franca, SERVILLO, Luigi, Chirico F, Caiazzo P, PAOLISSO, Giuseppe, MARFELLA, Raffaele, D'ONOFRIO, NUNZIA, Balestrieri, Maria Luisa, Rizzo, Maria Rosaria, Barbieri, Michelangela, Paolisso, P, D'Onofrio, N, Giovane, Alfonso, Siniscalchi, M, Minicucci, F, Sardu, C, D'Andrea, D, Mauro, C, Ferraraccio, Franca, Servillo, Luigi, Chirico, F, Caiazzo, P, Paolisso, Giuseppe, Marfella, Raffaele, and D'Onofrio, Nunzia
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Carotid Arterie ,Male ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Incretin ,Inflammation ,medicine.disease_cause ,Diabetes mellitus ,Internal medicine ,Receptors, Glucagon ,Internal Medicine ,medicine ,Sirtuin ,Progenitor cell ,Endarterectomy ,biology ,Liraglutide ,business.industry ,Medicine (all) ,medicine.disease ,Plaque, Atherosclerotic ,Endocrinology ,Diabetes Mellitus, Type 2 ,Dipeptidyl-Peptidase IV Inhibitor ,biology.protein ,Female ,medicine.symptom ,business ,Oxidative stress ,Human ,medicine.drug - Abstract
The role of sirtuin 6 (SIRT6) in atherosclerotic progression of diabetic patients is unknown. We evaluated SIRT6 expression and the effect of incretin-based therapies in carotid plaques of asymptomatic diabetic and nondiabetic patients. Plaques were obtained from 52 type 2 diabetic and 30 nondiabetic patients undergoing carotid endarterectomy. Twenty-two diabetic patients were treated with drugs that work on the incretin system, GLP-1 receptor agonists, and dipeptidyl peptidase-4 inhibitors for 26 ± 8 months before undergoing the endarterectomy. Compared with nondiabetic plaques, diabetic plaques had more inflammation and oxidative stress, along with a lesser SIRT6 expression and collagen content. Compared with non-GLP-1 therapy–treated plaques, GLP-1 therapy–treated plaques presented greater SIRT6 expression and collagen content, and less inflammation and oxidative stress, indicating a more stable plaque phenotype. These results were supported by in vitro observations on endothelial progenitor cells (EPCs) and endothelial cells (ECs). Indeed, both EPCs and ECs treated with high glucose (25 mmol/L) in the presence of GLP-1 (100 nmol/L liraglutide) presented a greater SIRT6 and lower nuclear factor-κB expression compared with cells treated only with high glucose. These findings establish the involvement of SIRT6 in the inflammatory pathways of diabetic atherosclerotic lesions and suggest its possible positive modulation by incretin, the effect of which is associated with morphological and compositional characteristics of a potential stable plaque phenotype.
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- 2014
10. Homostachydrine (pipecolic acid betaine) as authentication marker of roasted blends of Coffea arabica and Coffea canephora (Robusta) beans
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SERVILLO, Luigi, GIOVANE, Alfonso, CASALE, Rosario, Cautela, Domenico, D'Onofrio, Nunzia, BALESTRIERI, Maria Luisa, Castaldo, Domenico, D'ONOFRIO, NUNZIA, Servillo, Luigi, Giovane, Alfonso, Casale, Rosario, Cautela, Domenico, D'Onofrio, Nunzia, Balestrieri, Maria Luisa, and Castaldo, Domenico
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Spectrometry, Mass, Electrospray Ionization ,Arabica coffee ,Hot Temperature ,Food Handling ,Coffea ,Food Contamination ,Food chemistry ,Homostachydrine ,Coffea canephora ,Robusta coffee ,01 natural sciences ,Analytical Chemistry ,chemistry.chemical_compound ,0404 agricultural biotechnology ,Betaine ,Botany ,Pipecolic acid betaine ,Coffee bean ,Chromatography, High Pressure Liquid ,Roasting ,Pipecolic acid ,biology ,Chemistry ,Coffea arabica ,010401 analytical chemistry ,N-methylpipecolic acid ,Roasted coffee ,04 agricultural and veterinary sciences ,General Medicine ,LC-ESI-MS/MS ,biology.organism_classification ,040401 food science ,0104 chemical sciences ,Horticulture ,Pipecolic Acids ,Seeds ,Composition (visual arts) ,Food Science - Abstract
The occurrence of pipecolic acid betaine (homostachydrine) and its biosynthetic precursor N-methylpipecolic acid was detected for the first time in green coffee beans of Robusta and Arabica species. The analyses were conducted by HPLC-ESI tandem mass spectrometry and the metabolites identified by product ion spectra and comparison with authentic standards. N-methylpipecolic acid was found at similar levels in green coffee beans of Robusta and Arabica, whereas a noticeable difference of homostachydrine content was observed between the two green coffee bean species. Interestingly, homostachydrine content was found to be unaffected by coffee bean roasting treatment because of a noticeable heat stability, a feature that makes this compound a candidate marker to determine the content of Robusta and Arabica species in roasted coffee blends. To this end, a number of certified pure Arabica and Robusta green beans were analyzed for their homostachydrine content. Results showed that homostachydrine content was 1.5 ± 0.5 mg/kg in Arabica beans and 31.0 ± 10.0 mg/kg in Robusta beans. Finally, to further support the suitability of homostachydrine as quality marker of roasted blends of Arabica and Robusta coffee beans, commercial samples of roasted ground coffee blends were analyzed and the correspondence between the derived percentages of Arabica and Robusta beans with those declared on packages by manufacturers was verified.
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- 2015
11. Tyramine Pathways in Citrus Plant Defense: Glycoconjugates of Tyramine and Its N-Methylated Derivatives
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SERVILLO, Luigi, Castaldo, D, GIOVANE, Alfonso, CASALE, Rosario, D'Onofrio, N, Cautela, D, BALESTRIERI, Maria Luisa, D'ONOFRIO, NUNZIA, Servillo, Luigi, Castaldo, D, Giovane, Alfonso, Casale, Rosario, D'Onofrio, N, Cautela, D, Balestrieri, Maria Luisa, and D'Onofrio, Nunzia
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- 2017
12. Peri-procedural tight glycemic control during early percutaneous coronary intervention up-regulates endothelial progenitor cell level and differentiation during acute ST-elevation myocardial infarction: Effects on myocardial salvage
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MARFELLA, Raffaele, RIZZO, Maria Rosaria, Siniscalchi M, Paolisso P, BARBIERI, Michelangela, Sardu C, Savinelli A, Angelico N, Del Gaudio S, Esposito N, RAMBALDI, Pier Francesco, D'Onofrio N, MANSI, Luigi, Mauro C, PAOLISSO, Giuseppe, BALESTRIERI, Maria Luisa, D'ONOFRIO, NUNZIA, Marfella, Raffaele, Rizzo, Maria Rosaria, Siniscalchi, M, Paolisso, P, Barbieri, Michelangela, Sardu, C, Savinelli, A, Angelico, N, Del Gaudio, S, Esposito, N, Rambaldi, Pier Francesco, D'Onofrio, N, Mansi, Luigi, Mauro, C, Paolisso, Giuseppe, Balestrieri, Maria Luisa, and D'Onofrio, Nunzia
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Adult ,Blood Glucose ,Male ,medicine.medical_specialty ,Time Factors ,medicine.medical_treatment ,Peri ,Endothelial progenitor cell ,Myocardial infarction, PBMC, PCI, SIRT1 ,Percutaneous Coronary Intervention ,SIRT1 ,St elevation myocardial infarction ,Internal medicine ,Preoperative Care ,medicine ,Humans ,Prospective Studies ,Endothelial progenitor cells ,cardiovascular diseases ,Myocardial infarction ,Progenitor cell ,Cells, Cultured ,Aged ,Glycemic ,Salvage Therapy ,business.industry ,Myocardium ,Stem Cells ,Endothelial Cells ,Percutaneous coronary intervention ,Cell Differentiation ,Middle Aged ,medicine.disease ,Treatment Outcome ,surgical procedures, operative ,Glycemic Index ,myocardial salvage ,Conventional PCI ,Cardiology ,Female ,business ,Cardiology and Cardiovascular Medicine ,Follow-Up Studies - Abstract
BACKGROUND: We examined the effects of peri-procedural intensive glycemic control during early percutaneous coronary intervention (PCI) on the number and differentiation of endothelial progenitor cells (EPCs) and myocardial salvage (MS) in hyperglycemic patients with first ST-elevation myocardial infarction (STEMI). METHODS AND RESULTS: We conducted a randomized, prospective, open label study on 194 patients with STEMI undergoing PCI: 88 normoglycemic patients (glucose < 140 mg/dl) served as the control group. Hyperglycemic patients (glucose ≥140 mg/dl) were randomized to intensive glycemic control (IGC) for almost 24 h after PCI (n = 54; 80-140 mg/dl) or conventional glycemic control (CGC, n = 52; 180-200 mg/dl). EPC number, differentiation, and SIRT1expression were assessed immediately before, 24 h, 7, 30 and 180 days after PCI. The primary end point of the study was salvage index, measured as the proportion of initial perfusion defect (acute technetium-99m sestamibi scintigraphy, performed 5 to 7 days after STEMI) and myocardium salvaged by therapy (6 months after STEMI). Hyperglycemic patients had lower EPC number and differentiation and lower SIRT1 levels than normoglycemic patients (P < 0.01). After the insulin infusion, mean plasma glucose during peri-procedural period was greater in CGC group than in IGC group (P < 0.001). The EPC number, their capability to differentiate, and SIRT1 levels were significantly higher in IGC group than in CGC, peaking after 24 h (P < 0.01). In the IGC group, the salvage index was greater than in patients treated with CGC (P < 0.001). CONCLUSIONS: Optimal peri-procedural glycemic control, by increasing EPC number and their capability to differentiate, may improve the myocardial salvage.
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- 2013
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13. Multiple pathways of SIRT6 at the crossroads in the control of longevity, cancer, and cardiovascular diseases
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Vitiello M., Zullo, A, SERVILLO, Luigi, Mancini, FP, BORRIELLO, Adriana, GIOVANE, Alfonso, DELLA RAGIONE, Fulvio, D'Onofrio, N, BALESTRIERI, Maria Luisa, D'ONOFRIO, NUNZIA, Vitiello, M., Zullo, A, Servillo, Luigi, Mancini, Fp, Borriello, Adriana, Giovane, Alfonso, DELLA RAGIONE, Fulvio, D'Onofrio, N, Balestrieri, Maria Luisa, and D'Onofrio, Nunzia
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0301 basic medicine ,SIRT6 ,Aging ,DNA Repair ,DNA repair ,media_common.quotation_subject ,Longevity ,Inflammation ,Disease ,Biology ,Biochemistry ,03 medical and health sciences ,Neoplasms ,medicine ,Homeostasis ,Humans ,Sirtuins ,Molecular Biology ,Cancer ,media_common ,Lifespan ,Telomere Homeostasis ,Cardiovascular disease ,Telomere ,030104 developmental biology ,Neurology ,Cardiovascular Diseases ,Atherosclerosi ,Sirtuin ,Cancer research ,biology.protein ,medicine.symptom ,Biotechnology - Abstract
Sirtuin 6 (SIRT6) is a member of the sirtuin family NAD+-dependent deacetylases with multiple roles in controlling organism homeostasis, lifespan, and diseases. Due to its complex and opposite functional roles, this sirtuin is considered a two-edged sword in health and disease. Indeed, SIRT6 improves longevity, similarly to the founding yeast member, silent information regulator-2 (Sir2), and modulates genome stability, telomere integrity, transcription, and DNA repair. Its deficiency is associated with chronic inflammation, diabetes, cardiac hypertrophy, obesity, liver dysfunction, muscle/adipocyte disorders, and cancer. Besides, pieces of evidence showed that SIRT6 is a promoter of specific oncogenic pathways, thus disclosing its dual role regarding cancer development. Collectively, these findings suggest that multiple mechanisms, to date not entirely known, underlie the intriguing roles of SIRT6. Here we provide an overview of the current molecular mechanisms through which SIRT6 controls cancer and heart diseases, and describe its recent implications in the atherosclerotic plaque development.
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- 2016
14. Polyphenols, Oxidative Stress, and Vascular Damage in Diabetes
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MARFELLA, Raffaele, D'Onofrio, Nunzia, SIRANGELO, Ivana, RIZZO, Maria Rosaria, Capoluongo, Maria Carmela, SERVILLO, Luigi, PAOLISSO, Giuseppe, BALESTRIERI, Maria Luisa, D'ONOFRIO, NUNZIA, Several Authors, Victor R. Preedy, Marfella, Raffaele, D'Onofrio, Nunzia, Sirangelo, Ivana, Rizzo, Maria Rosaria, Capoluongo, Maria Carmela, Servillo, Luigi, Paolisso, Giuseppe, and Balestrieri, Maria Luisa
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Polyphenol ,Diabetes risk ,Medicine (miscellaneous) ,food and beverages ,Nitric oxide ,Vascular damage ,Biology ,Carbohydrate metabolism ,Pharmacology ,medicine.disease ,medicine.disease_cause ,Endothelial progenitor cell ,chemistry.chemical_compound ,chemistry ,Biochemistry ,Diabetes mellitus ,medicine ,Oxidative stre ,Soy milk ,Oxidative stress - Abstract
Oxidative stress induced by hyperglycemia is a key factor in the development and progression of diabetes and its vascular complications. Dietary polyphenols have received enormous attention because their consumption has been associated with lower rates of diabetes and cardiovascular diseases. These compounds are of plant origin, and are abundant in fruit, vegetables, chocolate, and nuts, as well as in beverages such as tea, coffee, wine, and soy milk. They can be divided into at least ten separate classes, four of which are important in the human diet: phenolic acids, flavonoids, stilbenes, and lignans. A number of in vitro and in vivo studies support the effect of polyphenols on glucose metabolism, diabetes risk, and diabetic impairments in the nitric oxide-mediated endothelial progenitor cell mobilization and homing. This chapter intends to review the current knowledge on polyphenols, oxidative stress, and vascular damage in diabetes, focusing on the most important and recent advances and challenges for future research.
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- 2014
15. Vascular-homing peptides for targeted drug delivery and molecular imaging: meeting the clinical challenges
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D'Onofrio N, CARAGLIA, Michele, Grimaldi A, MARFELLA, Raffaele, SERVILLO, Luigi, PAOLISSO, Giuseppe, BALESTRIERI, Maria Luisa, D'ONOFRIO, NUNZIA, D'Onofrio, N, Caraglia, Michele, Grimaldi, A, Marfella, Raffaele, Servillo, Luigi, Paolisso, Giuseppe, Balestrieri, Maria Luisa, and D'Onofrio, Nunzia
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Cancer Research ,Phage display ,Angiogenesis Inhibitors ,Diagnostic tools ,Bioinformatics ,Drug Delivery Systems ,In vivo ,Peptide Library ,Drug Discovery ,Genetics ,Medicine ,Animals ,Humans ,Molecular Targeted Therapy ,Peptide ligand ,Neovascularization, Pathologic ,business.industry ,Tumor endothelial cell ,Molecular Imaging ,Oncology ,Targeted drug delivery ,Immunology ,Endothelium, Vascular ,Molecular imaging ,business ,Peptides ,Homing (hematopoietic) - Abstract
The vasculature of each organ expresses distinct molecular signatures critically influenced by the pathological status. The heterogeneous profile of the vascular beds has been successfully unveiled by the in vivo phage display, a high-throughput tool for mapping normal, diseased, and tumor vasculature. Specific challenges of this growing field are targeted therapies against cancer and cardiovascular diseases, as well as novel bioimaging diagnostic tools. Tumor vasculature-homing peptides have been extensively evaluated in several preclinical and clinical studies both as targeted-therapy and diagnosis. To date, results from several Phase I and II trials have been reported and many other trials are currently ongoing or recruiting patients. In this review, advances in the identification of novel peptide ligands and their corresponding receptors on tumor endothelium through the in vivo phage display technology are discussed. Emphasis is given to recent findings in the clinical setting of vascular-homing peptides selected by in vivo phage display for the treatment of advanced malignancies and their altered vascular beds.
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- 2014
16. SIRT3 mediates the effects of PCSK9 inhibitors on inflammation, autophagy, and oxidative stress in endothelial cells
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Nunzia D'Onofrio, Francesco Prattichizzo, Raffaele Marfella, Celestino Sardu, Elisa Martino, Lucia Scisciola, Lorenza Marfella, Rosalba La Grotta, Chiara Frigé, Giuseppe Paolisso, Antonio Ceriello, Maria Luisa Balestrieri, D'Onofrio, Nunzia, Prattichizzo, Francesco, Marfella, Raffaele, Sardu, Celestino, Martino, Elisa, Scisciola, Lucia, Marfella, Lorenza, Grotta, Rosalba La, Frigé, Chiara, Paolisso, Giuseppe, Ceriello, Antonio, and Balestrieri, Maria Luisa
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Medicine (miscellaneous) ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) - Published
- 2023
17. Transcriptomic profiles of the ruminal wall in Italian Mediterranean dairy buffaloes fed green forage
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Salzano A, Fioriniello S, D'Onofrio N, Balestrieri ML, Aiese Cigliano R, Neglia G, Della Ragione F, Campanile G., Salzano, Angela, Fioriniello, Salvatore, D'Onofrio, Nunzia, Balestrieri, Maria Luisa, Aiese Cigliano, Riccardo, Neglia, Gianluca, Della Ragione, Floriana, Campanile, Giuseppe, Salzano, A, Fioriniello, S, D'Onofrio, N, Balestrieri, Ml, Aiese Cigliano, R, Neglia, G, Della Ragione, F, and Campanile, G.
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Transcriptome ,Rumen ,Green feed ,Genetics ,Buffalo ,RNA-seq ,Biotechnology - Abstract
Background Green feed diet in ruminants exerts a beneficial effect on rumen metabolism and enhances the content of milk nutraceutical quality. At present, a comprehensive analysis focused on the identification of genes, and therefore, biological processes modulated by the green feed in buffalo rumen has never been reported. We performed RNA-sequencing in the rumen of buffaloes fed a total mixed ration (TMR) + the inclusion of 30% of ryegrass green feed (treated) or TMR (control), and identified differentially expressed genes (DEGs) using EdgeR and NOISeq tools. Results We found 155 DEGs using EdgeR (p-values Gene Ontology analysis of DEGs identified using EdgeR revealed that green feed modulates biological processes relevant for the rumen physiology and, then, health and well-being of buffaloes, such as lipid metabolism, response to the oxidative stress, immune response, and muscle structure and function. Accordingly, we found: (i) up-regulation of HSD17B13, LOC102410803 (or PSAT1) and HYKK, and down-regulation of CDO1, SELENBP1 and PEMT, encoding factors involved in energy, lipid and amino acid metabolism; (ii) enhanced expression of SIM2 and TRIM14, whose products are implicated in the immune response and defense against infections, and reduced expression of LOC112585166 (or SAAL1), ROR2, SMOC2, and S100A11, encoding pro-inflammatory factors; (iii) up-regulation of NUDT18, DNAJA4 and HSF4, whose products counteract stressful conditions, and down-regulation of LOC102396388 (or UGT1A9) and LOC102413340 (or MRP4/ABCC4), encoding detoxifying factors; (iv) increased expression of KCNK10, CACNG4, and ATP2B4, encoding proteins modulating Ca2+ homeostasis, and reduced expression of the cytoskeleton-related MYH11 and DES. Conclusion Although statistically unpowered, this study suggests that green feed modulates the expression of genes involved in biological processes relevant for rumen functionality and physiology, and thus, for welfare and quality production in Italian Mediterranean dairy buffaloes. These findings, that need to be further confirmed through the validation of additional DEGs, allow to speculate a role of green feed in the production of nutraceutical molecules, whose levels might be enhanced also in milk.
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- 2023
18. SGLT-2 inhibitors and in-stent restenosis-related events after acute myocardial infarction: an observational study in patients with type 2 diabetes
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Raffaele Marfella, Celestino Sardu, Nunzia D’Onofrio, Carlo Fumagalli, Lucia Scisciola, Ferdinando Carlo Sasso, Mario Siniscalchi, Ludovica Vittoria Marfella, Davide D’Andrea, Fabio Minicucci, Giuseppe Signoriello, Arturo Cesaro, Maria Consiglia Trotta, Chiara Frigé, Francesco Prattichizzo, Maria Luisa Balestrieri, Antonio Ceriello, Paolo Calabrò, Ciro Mauro, Luca del Viscovo, Giuseppe Paolisso, Marfella, Raffaele, Sardu, Celestino, D'Onofrio, Nunzia, Fumagalli, Carlo, Scisciola, Lucia, Sasso, Ferdinando Carlo, Siniscalchi, Mario, Marfella, Ludovica Vittoria, D'Andrea, Davide, Minicucci, Fabio, Signoriello, Giuseppe, Cesaro, Arturo, Trotta, Maria Consiglia, Frigé, Chiara, Prattichizzo, Francesco, Balestrieri, Maria Luisa, Ceriello, Antonio, Calabrò, Paolo, Mauro, Ciro, Del Viscovo, Luca, and Paolisso, Giuseppe
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Glycemic control ,Major adverse cardiovascular event ,SGLT-2 inhibitor ,Restenosi ,Type 2 diabetes ,General Medicine - Abstract
Background No study evaluated the incidence of intra-stent restenosis (ISR)-related events in patients with type 2 diabetes (T2DM) and acute myocardial infarction (AMI) treated or not with sodium/glucose cotransporter 2 inhibitors (SGLT2i). Methods We recruited 377 patients with T2DM and AMI undergoing percutaneous coronary intervention (PCI). Among them, 177 T2DM were treated with SGLT2 inhibitors before PCI. The primary outcome was major adverse cardiovascular events (MACE) defined as cardiac death, re-infarction, and heart failure related to ISR. In patients without ISR, minimal lumen area and minimal lumen diameter were assessed by coronary CT-angiography at 1-year follow-up. Results Glycemic control was similar in SGLT2i-treated patients and never SGLT2i-users. The incidence of ISR-related MACE was higher in never SGLT2i-users compared with SGLT2i-treated patients, an effect independent of glycemic status (HR = 0.418, 95% CI = 0.241–0.725, P = 0.002) and observed also in the subgroup of patients with HbA1c < 7% (HR = 0.393, 95% CI = 0.157–0.984, P = 0.027). In patients without the event, the stent patency was greater in SGLT2i-treated patients compared with never SGLT2i-users at 1-year follow-up. Conclusions SGLT2i treatment in T2DM is associated with a reduced incidence of ISR-related events, independently of glycemic control.
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- 2023
19. Angiotensin receptor/Neprilysin inhibitor effects in CRTd non-responders: From epigenetic to clinical beside
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Celestino Sardu, Massimo Massetti, Lucia Scisciola, Maria Consiglia Trotta, Matteo Santamaria, Mario Volpicelli, Valentino Ducceschi, Giuseppe Signoriello, Nunzia D’Onofrio, Ludovica Marfella, Flavia Casolaro, Michele D.’ Amico, Antonio Ruocco, Maria Luisa Balestrieri, Ciro Mauro, Concetta Rafaniello, Annalisa Capuano, Giuseppe Paolisso, Raffaele Marfella, Sardu, Celestino, Massetti, Massimo, Scisciola, Lucia, Trotta, Maria Consiglia, Santamaria, Matteo, Volpicelli, Mario, Ducceschi, Valentino, Signoriello, Giuseppe, D'Onofrio, Nunzia, Marfella, Ludovica, Casolaro, Flavia, Amico, Michele D ', Ruocco, Antonio, Balestrieri, Maria Luisa, Mauro, Ciro, Rafaniello, Concetta, Capuano, Annalisa, Paolisso, Giuseppe, and Marfella, Raffaele
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CRTd non-responder ,Pharmacology ,Heart Failure ,Receptors, Angiotensin ,Ventricular Remodeling ,Clinical outcome ,MiRs regulation ,Stroke Volume ,HFrEF ,Epigenesis, Genetic ,Cardiac Resynchronization Therapy ,Angiotensin Receptor Antagonists ,Drug Combinations ,MicroRNAs ,Treatment Outcome ,Clinical outcomes ,Humans ,Neprilysin ,Settore MED/23 - CHIRURGIA CARDIACA ,CRTd non-responders ,Antihypertensive Agents - Abstract
We evaluated whether Angiotensin receptor/Neprilysin inhibitors (ARNI) reduce heart failure (HF) hospitalizations and deaths in cardiac resynchronization therapy with defibrillator (CRTd) non-responders patients at 12 months of follow-up, modulating microRNAs (miRs) implied in adverse cardiac remodeling.adverse cardiac remodeling characterized by left ventricle ejection fraction (LVEF) reduction, left ventricular end-systolic volume (LVESv) increase, and the 6-minute walking test (6MWT) reduction are relevant pathological mechanisms in CRTd non-responders and could be linked to changes in miRNAs (miRs), regulating cardiac fibrosis, apoptosis, and hypertrophy.miRs levels and clinical outcomes (LVEF, cardiac deaths, and 6MWT) were evaluated at baseline and one year of follow-up in CRTd non-responders divided into ARNI-users and Non-ARNI users.At baseline, there were no differences in levels of inflammatory markers, miR-18, miR-145, and miR-181 (p 0.05) between Non-ARNI users (n 106) and ARNI-users (n 312). At one year of follow-up, ARNI-users vs. Non-ARNI users showed lowest inflammatory markers (p 0.01) and miR-181 levels (p 0.01) and higher values of miR-18 (p 0.01)and miR-145 (p 0.01). At one year of follow-up, ARNI-users had a higher increase of LVEF (p 0.01) and 6MWT (p 0.01) along with a more significant reduction of LVESv (p 0.01) compared to Non-ARNI users. Cox regression analysis evidenced that ARNI-based therapies increase the probability of anti-remodeling effects of CRTd. Based on symptomatic improvements, echocardiographic and functional classification improvements, 37 (34.9%) patients among ARNI-users became responders, while only twenty (6.4%) patients became responders among Non-ARNi-users.ARNI might influence epigenetic mechanisms modulating miRs implicated in the adverse cardiac remodeling responses to CRTd.
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- 2022
20. Glycaemic control is associated with SARS-CoV-2 breakthrough infections in vaccinated patients with type 2 diabetes
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Raffaele Marfella, Celestino Sardu, Nunzia D’Onofrio, Francesco Prattichizzo, Lucia Scisciola, Vincenzo Messina, Rosalba La Grotta, Maria Luisa Balestrieri, Paolo Maggi, Claudio Napoli, Antonio Ceriello, Giuseppe Paolisso, Marfella, Raffaele, Sardu, Celestino, D'Onofrio, Nunzia, Prattichizzo, Francesco, Scisciola, Lucia, Messina, Vincenzo, La Grotta, Rosalba, Balestrieri, Maria Luisa, Maggi, Paolo, Napoli, Claudio, Ceriello, Antonio, and Paolisso, Giuseppe
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Glycated Hemoglobin ,COVID-19 Vaccines ,Glycated Hemoglobin A ,Multidisciplinary ,SARS-CoV-2 ,COVID-19 Vaccine ,COVID-19 ,General Physics and Astronomy ,Glycemic Control ,General Chemistry ,General Biochemistry, Genetics and Molecular Biology ,Diabetes Mellitus, Type 2 ,Humans ,Female ,RNA, Messenger ,BNT162 Vaccine ,Human - Abstract
Patients with type 2 diabetes (T2D) are characterized by blunted immune responses, which are affected by glycaemic control. Whether glycaemic control influences the response to COVID-19 vaccines and the incidence of SARS-CoV-2 breakthrough infections is unknown. Here we show that poor glycaemic control, assessed as mean HbA1c in the post-vaccination period, is associated with lower immune responses and an increased incidence of SARS-CoV-2 breakthrough infections in T2D patients vaccinated with mRNA-BNT162b2. We report data from a prospective observational study enroling healthcare and educator workers with T2D receiving the mRNA-BNT162b2 vaccine in Campania (Italy) and followed for one year (5 visits, follow-up 346 ± 49 days) after one full vaccination cycle. Considering the 494 subjects completing the study, patients with good glycaemic control (HbA1c one-year mean < 7%) show a higher virus-neutralizing antibody capacity and a better CD4 + T/cytokine response, compared with those with poor control (HbA1c one-year mean ≥ 7%). The one-year mean of HbA1c is linearly associated with the incidence of breakthrough infections (Beta = 0.068; 95% confidence interval [CI], 0.032-0.103; p p = 0.008). Among other factors, only smoking (HR = 0.290, CI 0.146-0.576 for non-smokers; p p
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- 2022
21. Incretin drugs effect on epigenetic machinery: New potential therapeutic implications in preventing vascular diabetic complications
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Giuseppe Paolisso, Rosaria Anna Fontanella, Michelangela Barbieri, Maria Luisa Balestrieri, Lucia Scisciola, Vittoria Cataldo, Raffaele Marfella, Maria Rosaria Rizzo, Nunzia D'Onofrio, Scisciola, Lucia, Rizzo, Maria Rosaria, Cataldo, Vittoria, Fontanella, Rosaria Anna, Balestrieri, Maria Luisa, D'Onofrio, Nunzia, Marfella, Raffaele, Paolisso, Giuseppe, and Barbieri, Michelangela
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Blood Glucose ,0301 basic medicine ,medicine.medical_specialty ,Methyltransferase ,SOD2 ,Gene Expression ,Incretin ,Type 2 diabetes ,Carotid Intima-Media Thickness ,Incretins ,Biochemistry ,Glucagon-Like Peptide-1 Receptor ,Body Mass Index ,Cell Line ,Epigenesis, Genetic ,Diabetes Complications ,03 medical and health sciences ,incretin based drug ,0302 clinical medicine ,Internal medicine ,Genetics ,Humans ,Medicine ,Epigenetics ,Molecular Biology ,Superoxide Dismutase ,business.industry ,NF-kappa B ,Endothelial Cells ,Methylation ,DNA Methylation ,Atherosclerosis ,medicine.disease ,Glucose ,030104 developmental biology ,Endocrinology ,Diabetes Mellitus, Type 2 ,Intima-media thickness ,DNA methylation ,atherosclerosis progression ,cardiovascular system ,type 2 diabetes ,business ,030217 neurology & neurosurgery ,Biotechnology - Abstract
The effect of GLP-1R agonists on DNA methylation levels of NF-κB and SOD2 genes in human aortic endothelial cells exposed to high glucose and in diabetic patients treated and not with incretin-based drugs, was evaluated. Methylation levels, mRNA and protein expression of NF-κB and SOD2 genes were measured in human endothelial cells exposed to high glucose for 7days and treated with GLP-1R agonists. Methylation status of NF-κB and SOD2 promoter was also analyzed in 128 diabetics and 116 nondiabetics and correlated with intima media thickness (ITM), an early marker of atherosclerotic process. Cells exposed to high glucose showed lower NF-κB and SOD2 methylation levels, increased NF-κB and reduced SOD2 expression compared to normal glucose cells. Co-treatment with GLP-1 agonists prevented methylation and genes expression changes induced by high glucose. Both high glucose and incretins exposure increased DNA methyltransferases and demethylases levels. In diabetics, incretin treatment resulted a significant predictor of NF-κB DNA methylation, independently of age, sex, body mass index (BMI), glucose and plasma lipid levels. NF-κB DNA methylation inversely correlated with IMT after adjusting for multiple covariates. Our results firstly provide new evidences of an additional mechanism by which incretin drugs could prevent vascular diabetic complications.
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- 2020
22. Outcomes in Patients With Hyperglycemia Affected by COVID-19: Can We Do More on Glycemic Control?
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Michelangela Barbieri, Paolo Maggi, Nunzia D'Onofrio, Maria Luisa Balestrieri, Raffaele Marfella, Celestino Sardu, Vincenzo Messina, Nicola Coppola, Maria Rosaria Rizzo, Giuseppe Paolisso, Sardu, Celestino, D'Onofrio, Nunzia, Balestrieri, Maria Luisa, Barbieri, Michelangela, Rizzo, Maria Rosaria, Messina, Vincenzo, Maggi, Paolo, Coppola, Nicola, Paolisso, Giuseppe, and Marfella, Raffaele
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Blood Glucose ,Male ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Pneumonia, Viral ,Diabetes and COVID-19—Higher Risks and Many Questions ,030209 endocrinology & metabolism ,Disease ,Gastroenterology ,law.invention ,Betacoronavirus ,03 medical and health sciences ,0302 clinical medicine ,law ,Internal medicine ,Diabetes mellitus ,Internal Medicine ,medicine ,Humans ,Hypoglycemic Agents ,Insulin ,030212 general & internal medicine ,Interleukin 6 ,Pandemics ,Glycemic ,Advanced and Specialized Nursing ,Mechanical ventilation ,biology ,SARS-CoV-2 ,business.industry ,COVID-19 ,Type 2 Diabetes Mellitus ,Middle Aged ,medicine.disease ,Intensive care unit ,Hospitalization ,Intensive Care Units ,Hyperglycemia ,biology.protein ,Female ,Coronavirus Infections ,business - Abstract
OBJECTIVE An important prognostic factor in any form of infection seems to be glucose control in patients with type 2 diabetes. There is no information about the effects of tight glycemic control on coronavirus disease 2019 (COVID-19) outcomes in patients with hyperglycemia. Therefore, we examined the effects of optimal glycemic control in patients with hyperglycemia affected by COVID-19. RESEARCH DESIGN AND METHODS Fifty-nine patients with COVID-19 hospitalized with moderate disease were evaluated. On the basis of admission glycemia >7.77 mmol/L, patients were divided into hyperglycemic and normoglycemic groups. Interleukin 6 (IL-6) and D-dimer levels were evaluated at admission and weekly during hospitalization. The composite end point was severe disease, admission to an intensive care unit, use of mechanical ventilation, or death. RESULTS Thirty-four (57.6%) patients were normoglycemic and 25 (42.4%) were hyperglycemic. In the hyperglycemic group, 7 (28%) and 18 (72%) patients were diagnosed with diabetes already before admission, and 10 (40%) and 15 (60%) were treated without and with insulin infusion, respectively. The mean of glycemia during hospitalization was 10.65 ± 0.84 mmol/L in the no insulin infusion group and 7.69 ± 1.85 mmol/L in the insulin infusion group. At baseline, IL-6 and D-dimer levels were significantly higher in the hyperglycemic group than in the normoglycemic group (P < 0.001). Even though all patients were on standard treatment for COVID-19 infection, IL-6 and D-dimer levels persisted higher in patients with hyperglycemia during hospitalization. In a risk-adjusted Cox regression analysis, both patients with hyperglycemia and patients with diabetes had a higher risk of severe disease than those without diabetes and with normoglycemia. Cox regression analysis evidenced that patients with hyperglycemia treated with insulin infusion had a lower risk of severe disease than patients without insulin infusion. CONCLUSIONS Insulin infusion may be an effective method for achieving glycemic targets and improving outcomes in patients with COVID-19.
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- 2020
23. Lipid Accumulation in Hearts Transplanted From Nondiabetic Donors to Diabetic Recipients
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Cristiano Amarelli, Paolo Golino, Gelsomina Mansueto, Michele D'Amico, Ciro Maiello, Claudio Napoli, Salvatore Esposito, Irene Mattucci, Nunzia D'Onofrio, Giuseppe Paolisso, Maria Luisa Balestrieri, Raffaele Marfella, Marisa De Feo, Francesco Cacciatore, Gemma Salerno, Marfella, Raffaele, Amarelli, Cristiano, Cacciatore, Francesco, Balestrieri, Maria Luisa, Mansueto, Gelsomina, D'Onofrio, Nunzia, Esposito, Salvatore, Mattucci, Irene, Salerno, Gemma, De Feo, Marisa, D'Amico, Michele, Golino, Paolo, Maiello, Ciro, Paolisso, Giuseppe, Napoli, Claudio, Marfella, R., Amarelli, C., Cacciatore, F., Balestrieri, M. L., Mansueto, G., D'Onofrio, N., Esposito, S., Mattucci, I., Salerno, G., De Feo, M., D'Amico, M., Golino, P., Maiello, C., Paolisso, G., and Napoli, C.
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Male ,medicine.medical_specialty ,Diabetic Cardiomyopathies ,Heart Ventricles ,medicine.medical_treatment ,Context (language use) ,030204 cardiovascular system & hematology ,heart transplantation ,Gastroenterology ,Follow-Up Studie ,Heart Ventricle ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Insulin resistance ,Diabetes mellitus ,Internal medicine ,DMCM ,Diabetic cardiomyopathy ,diabetic cardiomyopathy ,medicine ,Humans ,Hypoglycemic Agents ,Myocytes, Cardiac ,Prospective Studies ,030212 general & internal medicine ,Diabetic Cardiomyopathie ,Heart Failure ,Heart transplantation ,Hypoglycemic Agent ,business.industry ,Middle Aged ,CVD ,Lipid Metabolism ,medicine.disease ,Metformin ,Prospective Studie ,Diabetes Mellitus, Type 2 ,Lipotoxicity ,chemistry ,diabete ,Female ,Cardiology and Cardiovascular Medicine ,business ,Follow-Up Studies ,Human ,medicine.drug - Abstract
Background: Early pathogenesis of diabetic cardiomyopathy (DMCM) may involve lipotoxicity of cardiomyocytes in the context of hyperglycemia. There are many preclinical studies of DMCM pathogenesis, but the human evidence is still poorly understood. Objectives: By using a nondiabetic mellitus (non-DM) heart transplanted (HTX) in diabetes mellitus (DM) recipients, this study conducted a serial study of human heart transplant recipients evaluating cardiac effects of diabetic milieu (hyperglycemia and insulin resistance) on lipotoxic-mediated injury. We evaluated cardiomyocyte morpho-pathology by seriated biopsies of healthy implanted hearts in DM recipients during 12-month follow-up from HTX. Because metformin reduces ectopic lipid accumulation, we evaluated the effects of the drug in a nonrandomized subgroup. Methods: The DMCM-AHEAD (Diabetes and Lipid Accumulation and Heart Transplant) prospective ongoing study (NCT03546062) evaluated 158 first HTX recipients (82 non-DM, 76 DM of whom 35 [46%] were receiving metformin). HTX recipients were undergoing clinical standard evaluation (metabolic status, echocardiography, coronary computed tomography angiography, and endomyocardial biopsies). Biopsies evaluated immune response, Oil Red-O staining, ceramide, and triacylglycerol levels. Lipotoxic factors and insulin resistance were evaluated by reverse transcriptase–polymerase chain reaction. Results: There was a significant early and progressive cardiomyocyte lipid accumulation in DM but not in non-DM recipients (p = 0.019). In the subgroup receiving metformin, independently from immunosuppressive therapy that was similar among groups, lipid accumulation was reduced in comparison with DM recipients not receiving the drug (hazard ratio: 6.597; 95% confidence interval: 2.516 to 17.296; p < 0.001). Accordingly, lipotoxic factors were increased in DM versus non-DM recipients, and, relevantly, metformin use was associated with fewer lipotoxic factors. Conclusions: Early pathogenesis of human DMCM started with cardiomyocyte lipid accumulation following HTX in DM recipients. Metformin use was associated with reduced lipid accumulation independently of immunosuppressive therapy. This may constitute a novel target for therapy of DMCM.
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- 2020
24. Does poor glycaemic control affect the immunogenicity of the COVID-19 vaccination in patients with type 2 diabetes: The CAVEAT study
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Giovanni Napolitano, Gianpaolo Papaccio, Fabrizio Turriziani, Celestino Sardu, Marilena Galdiero, Antonio Papa, Luigi Salemme, Giuseppe Paolisso, Lucia Scisciola, Nicola Coppola, Eugenio Basile, Carmela Papa, Paolo Maggi, Michelangela Barbieri, Ferdinando Carlo Sasso, Ciro Romano, F. Russo, Maria Rosaria Rizzo, Maria Luisa Balestrieri, Raffaele Marfella, Mario Siniscalchi, Claudio Napoli, Vincenzo Messina, Nunzia D'Onofrio, Ludovica Vittoria Marfella, Maria Vittoria Montemurro, Italo F. Angelillo, Virginia Tirino, Marco Boccalatte, Francesco Frascaria, Mauro Maniscalco, Marfella, Raffaele, D'Onofrio, Nunzia, Sardu, Celestino, Scisciola, Lucia, Maggi, Paolo, Coppola, Nicola, Romano, Ciro, Messina, Vincenzo, Turriziani, Fabrizio, Siniscalchi, Mario, Maniscalco, Mauro, Boccalatte, Marco, Napolitano, Giovanni, Salemme, Luigi, Marfella, Ludovica Vittoria, Basile, Eugenio, Montemurro, Maria Vittoria, Papa, Carmela, Frascaria, Francesco, Papa, Antonio, Russo, Ferdinando, Tirino, Virginia, Papaccio, Gianpaolo, Galdiero, Marilena, Sasso, Ferdinando Carlo, Barbieri, Michelangela, Rizzo, Maria Rosaria, Balestrieri, Maria Luisa, Angelillo, Italo Francesco, Napoli, Claudio, Paolisso, Giuseppe, Marfella, R., D'Onofrio, N., Sardu, C., Scisciola, L., Maggi, P., Coppola, N., Romano, C., Messina, V., Turriziani, F., Siniscalchi, M., Maniscalco, M., Boccalatte, M., Napolitano, G., Salemme, L., Marfella, L. V., Basile, E., Montemurro, M. V., Papa, C., Frascaria, F., Papa, A., Russo, F., Tirino, V., Papaccio, G., Galdiero, M., Sasso, F. C., Barbieri, M., Rizzo, M. R., Balestrieri, M. L., Angelillo, I. F., Napoli, C., and Paolisso, G.
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Blood Glucose ,medicine.medical_specialty ,Glycated Hemoglobin A ,COVID-19 Vaccines ,Coronavirus disease 2019 (COVID-19) ,COVID-19 Vaccine ,Endocrinology, Diabetes and Metabolism ,MEDLINE ,Type 2 diabetes ,Glycemic Control ,Affect (psychology) ,Endocrinology ,Immunogenicity, Vaccine ,Internal medicine ,Research Letter ,Internal Medicine ,medicine ,Humans ,In patient ,Poor glycaemic control ,Glycated Hemoglobin ,business.industry ,Immunogenicity ,COVID-19 ,medicine.disease ,Research Letters ,Vaccination ,Diabetes Mellitus, Type 2 ,business ,Human - Published
- 2021
25. Breed and Feeding System Impact the Bioactive Anti-Inflammatory Properties of Bovine Milk
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Angela Salzano, Maria Chiara Di Meo, Nunzia D’Onofrio, Giovanna Bifulco, Alessio Cotticelli, Francesca Licitra, Antonio Iraci Fuintino, Giuseppe Cascone, Maria Luisa Balestrieri, Ettore Varricchio, Giuseppe Campanile, Salzano, A, Di Meo, Mc, D'Onofrio, N, Bifulco, G, Cotticelli, A, Licitra, F, Iraci Fuintino, A, Cascone, G, Balestrieri, Ml, Varricchio, E, Campanile, G., Salzano, Angela, Chiara Di Meo, Maria, D'Onofrio, Nunzia, Bifulco, Giovanna, Cotticelli, Alessio, Licitra, Francesca, Iraci Fuintino, Antonio, Cascone, Giuseppe, Balestrieri, MARIA LUISA, Varricchio, Ettore, and Campanile, Giuseppe
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antioxidant ,Anti-Inflammatory Agents ,Antioxidants ,Catalysis ,Inorganic Chemistry ,Carnitine ,Animals ,Lactation ,Physical and Theoretical Chemistry ,Molecular Biology ,Spectroscopy ,dairy cattle ,Modicana ,milk ,betaine ,Interleukin-6 ,Organic Chemistry ,General Medicine ,Animal Feed ,Diet ,Computer Science Applications ,Betaine ,Milk ,Whey Proteins ,Cattle ,Female ,Acetylcarnitine ,Reactive Oxygen Species - Abstract
In the present study, we aimed at assessing the influence of breed and feeding system on the bovine milk profile of betaines and carnitines and milk capacity in counteracting the inflammatory endothelial cell (EC) damage induced by interleukin (IL)-6. In the first experimental design, two breeds were chosen (Holstein vs. Modicana) to investigate the biomolecule content and antioxidant capacity in milk and dairy products. In the second experimental design, two feeding systems (pasture vs. total mixed ratio) were tested only in Holstein to evaluate the possible effect on the functional profile of milk and dairy products. Finally, the bulk milk from the two experimental designs was used to evaluate the efficacy of preventing IL-6-induced endothelial inflammatory damage. Results showed that Modicana milk and whey had higher biomolecule content and antioxidant activity compared to Holstein milk (p < 0.01). Milk from Holstein fed TMR showed higher concentration of γ-butyrobetaine, δ-valerobetaine (p < 0.01), and l-carnitine (p < 0.05). Similarly, whey from Holstein fed TMR also showed higher content of δ-valerobetaine, glycine betaine, l-carnitine, and acetyl-l-carnitine (p < 0.01) compared to the Holstein fed pasture. Conversely, the antioxidant activity of milk and dairy products was not affected by the feeding system. In ECs, all milk samples reduced the IL-6-induced cytokine release, as well as the accumulation of reactive oxygen species (ROS) and the induction of cell death, with the most robust effect elicited by Modicana milk (p < 0.01). Overall, Modicana milk showed a higher content of biomolecules and antioxidant activity compared to Holstein, suggesting that the breed, more than the feeding system, can positively affect the health-promoting profile of dairy cattle milk.
- Published
- 2022
26. MicroRNA‐33 and SIRT1 influence the coronary thrombus burden in hyperglycemic STEMI patients
- Author
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Iacopo Panarese, Pasquale Paolisso, Michelangela Barbieri, Federica Varavallo, Nunzia D'Onofrio, Maria Luisa Balestrieri, Giuseppina Brunitto, Raffaele Marfella, Maria Rosaria Rizzo, Giuseppe Paolisso, Franca Ferraraccio, Gianpaolo Papaccio, Virginia Tirino, Ciro Mauro, Gelsomina Mansueto, Felice Gragnano, Celestino Sardu, Rosanna Caserta, Fabio Minicucci, Lucia Scisciola, D'Onofrio, Nunzia, Sardu, Celestino, Paolisso, Pasquale, Minicucci, Fabio, Gragnano, Felice, Ferraraccio, Franca, Panarese, Iacopo, Scisciola, Lucia, Mauro, Ciro, Rizzo, Maria Rosaria, Mansueto, Gelsomina, Varavallo, Federica, Brunitto, Giuseppina, Caserta, Rosanna, Tirino, Virginia, Papaccio, Gianpaolo, Barbieri, Michelangela, Paolisso, Giuseppe, Balestrieri, Maria Luisa, and Marfella, Raffaele
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Physiology ,medicine.medical_treatment ,Clinical Biochemistry ,Myocardial Infarction ,medicine.disease_cause ,Cell Line ,Cohort Studies ,STEMI ,03 medical and health sciences ,SIRT1 ,0302 clinical medicine ,Sirtuin 1 ,Coronary thrombus ,Internal medicine ,microRNA ,medicine ,Humans ,oxidative stress ,Gene Silencing ,cardiovascular diseases ,Myocardial infarction ,biology ,business.industry ,Coronary Thrombosis ,Incidence (epidemiology) ,Causal effect ,Endothelial Cells ,Percutaneous coronary intervention ,Cell Biology ,medicine.disease ,MicroRNAs ,miR33 ,030104 developmental biology ,030220 oncology & carcinogenesis ,Cardiology ,biology.protein ,hyperglycemia ,business ,Oxidative stress - Abstract
Primary percutaneous coronary intervention (PPCI) is a pivotal treatment in ST-segment elevation myocardial infarction (STEMI) patients. However, in hyperglycemic-STEMI patients, the incidence of death is still significant. Here, the involvement of sirtuin 1 (SIRT1) and miR33 on the pro-inflammatory/pro-coagulable state of the coronary thrombus was investigated. Moreover, 1-year outcomes in hyperglycemic STEMI in patients subjected to thrombus aspiration before PPCI were evaluated. Results showed that hyperglycemic thrombi displayed higher size and increased miR33, reactive oxygen species, and pro-inflammatory/pro-coagulable markers. Conversely, the hyperglycemic thrombi showed a lower endothelial SIRT1 expression. Moreover, in vitro experiments on endothelial cells showed a causal effect of SIRT1 modulation on the pro-inflammatory/pro-coagulative state via hyperglycemia-induced miR33 expression. Finally, SIRT1 expression negatively correlated with STEMI outcomes. These observations demonstrate the involvement of the miR33/SIRT1 pathway in the increased pro-inflammatory and pro-coagulable state of coronary thrombi in hyperglycemic STEMI patients.
- Published
- 2019
27. Effects of Metformin Therapy on Coronary Endothelial Dysfunction in Patients With Prediabetes With Stable Angina and Nonobstructive Coronary Artery Stenosis: The CODYCE Multicenter Prospective Study
- Author
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Paolo Calabrò, Celestino Sardu, Ciro Mauro, Maria Rosaria Rizzo, Ferdinando Carlo Sasso, Cosimo Sacra, Michelangela Barbieri, Maria Luisa Balestrieri, Raffaele Marfella, Pasquale Paolisso, Giuseppe Paolisso, Fabio Minicucci, Nunzia D'Onofrio, Michele Portoghese, Sardu, Celestino, Paolisso, Pasquale, Sacra, Cosimo, Mauro, Ciro, Minicucci, Fabio, Portoghese, Michele, Rizzo, Maria Rosaria, Barbieri, Michelangela, Sasso, Ferdinando Carlo, D'Onofrio, Nunzia, Balestrieri, Maria Luisa, Calabro', Paolo, Paolisso, Giuseppe, and Marfella, Raffaele
- Subjects
Male ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Coronary Angiography ,Prediabetic State ,Angina ,Internal medicine ,Internal Medicine ,medicine ,Humans ,Angina, Stable ,Prospective Studies ,Prediabetes ,Myocardial infarction ,Endothelial dysfunction ,Aged ,Advanced and Specialized Nursing ,business.industry ,Coronary Stenosis ,Heart ,Middle Aged ,medicine.disease ,Coronary Vessels ,Metformin ,Stenosis ,Treatment Outcome ,Heart failure ,Cardiology ,Female ,Endothelium, Vascular ,business ,Mace ,medicine.drug - Abstract
OBJECTIVE To evaluate the effect of metformin therapy on coronary endothelial function and major adverse cardiac events (MACE) in patients with prediabetes with stable angina and nonobstructive coronary stenosis (NOCS). RESEARCH DESIGN AND METHODS Metformin therapy may be needed to reduce coronary heart disease risk in patients with prediabetes. A total of 258 propensity score–matched (PSM) patients with stable angina undergoing coronary angiography were enrolled in the study. Data from 86 PSM subjects with normoglycemia (NG), 86 PSM subjects with prediabetes (pre-DM), and 86 PSM subjects with prediabetes treated with metformin (pre-DM metformin) were analyzed. During coronary angiography, NOCS was categorized by luminal stenosis 0.80. In addition, we assessed the endothelial function, measuring coronary artery diameter of left anterior descending coronary (LAD) at baseline and after the infusion of acetylcholine, by means of an intracoronary Doppler guide wire. MACE, as cardiac death, myocardial infarction, and heart failure, was evaluated at 24 months of follow-up. RESULTS At baseline, NG patients had a lower percentage of LAD endothelial dysfunction compared with pre-DM patients (P < 0.05). The pre-DM patients had a higher percentage of endothelial LAD dysfunction as compared with the pre-DM metformin patients (P < 0.05). At the 24th month of follow-up, MACE was higher in pre-DM versus NG (P < 0.05). In pre-DM metformin patients, MACE was lower compared with pre-DM patients (P < 0.05). CONCLUSIONS Metformin therapy may reduce the high risk of cardiovascular events in pre-DM patients by reducing coronary endothelial dysfunction.
- Published
- 2019
28. ROS-Mediated Apoptotic Cell Death of Human Colon Cancer LoVo Cells by Milk δ-Valerobetaine
- Author
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Gianluca Neglia, Assunta Lombardi, Nunzia D'Onofrio, Elisa Martino, Nunzio Antonio Cacciola, Maria Luisa Balestrieri, Ferdinando Fiorino, Giuseppe Campanile, Francesca Borrelli, D'Onofrio, N., Cacciola, N. A., Martino, E., Borrelli, F., Fiorino, F., Lombardi, A., Neglia, G., Balestrieri, M. L., Campanile, G., D'Onofrio, Nunzia, Antonio Cacciola, Nunzio, Martino, Elisa, Borrelli, Francesca, Fiorino, Ferdinando, Lombardi, Assunta, Neglia, Gianluca, Balestrieri, Maria Luisa, and Campanile., Giuseppe
- Subjects
0301 basic medicine ,Cell biology ,Necrosis ,Cell Survival ,Molecular biology ,Cell ,Cyclin A ,lcsh:Medicine ,Caspase 3 ,Antineoplastic Agents ,Apoptosis ,Adenocarcinoma ,Biochemistry ,Article ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Autophagy ,Tumor Cells, Cultured ,Animals ,Humans ,Sirtuins ,Cyclin B1 ,lcsh:Science ,Cancer ,buffalo ,Multidisciplinary ,biology ,Dose-Response Relationship, Drug ,Chemistry ,lcsh:R ,Cell Cycle ,Cell cycle ,δ-Valerobetaine ,Up-Regulation ,Betaine ,030104 developmental biology ,medicine.anatomical_structure ,Milk ,030220 oncology & carcinogenesis ,Cancer cell ,Colonic Neoplasms ,biology.protein ,lcsh:Q ,medicine.symptom ,Reactive Oxygen Species - Abstract
δ-Valerobetaine (δVB) is a constitutive milk metabolite with antioxidant and anti-inflammatory activities. Here, we tested the antineoplastic properties of milk δVB on human colorectal cancer cells. CCD 841 CoN (non-tumorigenic), HT-29 (p53 mutant adenocarcinoma) and LoVo (APC/RAS mutant adenocarcinoma) cells were exposed to 3 kDa milk extract, δVB (2 mM) or milk+δVB up to 72 h. Results showed a time- and dose-dependent capability of δVB to inhibit cancer cell viability, with higher potency in LoVo cells. Treatment with milk+δVB arrested cell cycle in G2/M and SubG1 phases by upregulating p21, cyclin A, cyclin B1 and p53 protein expressions. Noteworthy, δVB also increased necrosis (P P P SIRT6 silencing by small interfering RNA blocked autophagy and apoptosis activated by milk+δVB, unveiling the role of this sirtuin in the ROS-mediated apoptotic LoVo cell death.
- Published
- 2019
29. Thrombus Aspiration in Hyperglycemic Patients With High Inflammation Levels in Coronary Thrombus
- Author
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Nunzia D'Onofrio, Maria Luisa Balestrieri, Ciro Mauro, Raffaele Marfella, Celestino Sardu, Sardu, Celestino, D'Onofrio, Nunzia, Mauro, Ciro, Balestrieri, Maria Luisa, and Marfella, Raffaele
- Subjects
Inflammation ,medicine.medical_specialty ,Thrombus aspiration ,business.industry ,Coronary Thrombosis ,030204 cardiovascular system & hematology ,03 medical and health sciences ,0302 clinical medicine ,Coronary thrombosis ,Coronary thrombus ,Thrombus burden ,Internal medicine ,cardiovascular system ,medicine ,Cardiology ,Humans ,In patient ,cardiovascular diseases ,030212 general & internal medicine ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,Thrombectomy ,circulatory and respiratory physiology - Abstract
The carefully conducted study by Jolly et al. [(1)][1] reports that in patients with high thrombus burden, routine thrombus aspiration (TA) did not improve outcomes at 1 year. Nevertheless, the authors outlined that high thrombus burden is still an important predictor of outcome in ST-segment
- Published
- 2019
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