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1. Results of microsurgical epididymal sperm aspiration (MESA) ans testicular sperm extraction (TESE) in azoospermic men using intracytoplasmic sperm injection (ICSI)

Author

Ubaldi F, Camus M, Herman Tournaye, Clasen K, Nagy Z, Smitz J, Van Steirteghem A, and Devroey P

Subjects
Epididymis, Male, Microsurgery, Inhalation, Pregnancy, Testis, Sperm Motility, Humans, Female, Cell Separation, Fertilization in Vitro, Oligospermia, Spermatozoa
Abstract

The rationale and results of using epididymal and testicular spermatozoa with intracytoplasmic sperm injection (ICSI) for zoospermic patients are reviewed. A total of 128 consecutive ICSI/MESA cycles and a total of 120 consecutive ICSI/TESE cycles were performed up to December 1994. The two-pronuclei fertilization rate per intact oocyte (observed after the injection) was 58% and 60%, respectively, when epididymal and testicular spermatozoa were used. The embryo transfer rate was similar for the two procedures (91% after ICSI/MESA and 90% after ICSI/TESE). Fifty women became pregnant (positive HCG) when epididymal spermatozoa were used (39% per cycle and 40% per embryo transfer). These results are comparable to those obtained when ejaculated spermatozoa are used.

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