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2 results on '"Cian Holohan"'

1. Role of detergents and nuclease inhibitors in the extraction of RNA from eukaryotic cells in complex matrices

Author

Cian Holohan, Nathan Feely, Peng Li, Gerard Curran, and Gil U. Lee

Subjects
Eukaryotic Cells, Ribonucleases, Octoxynol, Nucleic Acids, Detergents, Humans, Poloxalene, RNA, General Materials Science, Melanoma
Abstract

The potential for liquid biopsy samples to be used in place of more invasive tissue biopsies has become increasingly revalent as it has been found that nucleic acids (NAs) present in the blood of cancer patients originate from tumors. Nanomagnetic extraction has proven to be a highly effective means to rapidly prepare NA from clinical samples for molecular diagnostics. In this article, the lysis reaction used to extract RNA from the human epithelial melanoma cells have been optimized using silica coated superparamagnetic nanoparticles (SPM NP). The lysis buffer (LB) is composed of several agents that denature cells

Published
2022

2. Draft Genome Sequences of Two Natural Isolates of the Yeast Barnettozyma californica from Ireland, UCD09 and UCD89

Author

Tadhg Ó Cróinín, Geraldine Butler, Shannon M. Hill, Cian Holohan, Alisha A. Mullen, Ciara D. Lynch, Caoimhe E. O’Brien, and Kenneth H. Wolfe

Subjects
0301 basic medicine, Genetics, Whole genome sequencing, Eukaryotes, fungi, Single-nucleotide polymorphism, Biology, Barnettozyma californica, Genome, Yeast, 03 medical and health sciences, 030104 developmental biology, Genus, SNP, Ploidy, Molecular Biology
Abstract

No genome sequence of a species from Barnettozyma , a yeast genus in the family Phaffomycetaceae, is currently available. We isolated two B. californica strains from soils in Ireland and generated draft sequences of their 11.7-Mb genomes. Single nucleotide polymorphism (SNP) analysis showed 20,490 differences between the strains and suggests that B. californica is haploid.

Published
2018

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