55 results on '"Chuan, Zuo"'
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2. Can the Urban Growth Boundary Reduce Urban Industrial Air Pollution in China? A Study from the Perspective of the Sustainability of Land Urbanization
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Jun Li and Chuan Zuo
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Environmental Chemistry ,General Environmental Science - Published
- 2022
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Catalog
3. The Effects of Extracts from 'Red Complex' Pathogens on Human Dental Follicle Cells
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Shuang Wu, Yi Zhou, Dong-Chuan Zuo, Yueyue Huang, Xiaoping Yuan, Yue Zhou, Xiao-Mei Xu, and Jin Zeng
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stomatognathic diseases ,Dental follicle ,Biomedical Engineering ,Medicine (miscellaneous) ,Bioengineering ,Biology ,Red complex ,Biotechnology ,Microbiology - Abstract
Dental follicle cells could be a natural source of cells for regeneration therapies in periodontitis. The "red complex", which include Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia, is strongly associated with periodontitis. However, the effects of "red complex" on dental follicle cells remains largely unknown. In this study, we evaluated the effects of bacterial extracts obtained from Porphyromonas gingivalis and from "red complex" (Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia in co-culture) on proliferation, migration and osteogenic differentiation of human dental follicle cells. Our data showed that bacterial extracts promoted cell proliferation and migration, decreased the alkaline phosphatase activity, osteogenic genes expression and formation of mineral deposits. Noticeably, compared with the inhibitory effects induced by bacterial extracts from Porphyromonas gingivalis on osteogenic differentiation of human dental follicle cells, the inhibitory effects induced by bacterial extracts from "red complex" were more significant. In conclusion, the extracts from "red complex" pathogens inhibited osteogenic differentiation capacity of human dental follicle cells, and this provides a theoretical basis for the clinical application of stem cell therapy in periodontal tissue engineering. more...
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- 2020
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4. [Effect of calcium on proliferation, migration and osteogenic differentiation of human dental follicle cells]
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Xian, Zhao, Jin, Zeng, Dong-Chuan, Zuo, and Xiao-Mei, Xu
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Osteogenesis ,Humans ,Calcium ,Cell Differentiation ,Dental Sac ,Cells, Cultured ,Cell Proliferation - Abstract
To determine the role of CahDFCs were isolated and cultured. The source of hDFCs was detected by immunofluorescence staining. Osteogenesis and adipogenic differentiation of hDFCs was detected by alizarin red staining and oil red O staining, to identify its multi-directional differentiation ability. A series of Ca2+ solutions with different concentrations was prepared, CCK8 assay was used to detect the proliferative abilities at 1, 3, 5, and 7 d;migratory ability of 24 h was detected by Transwell assay. Calcium nodules were detected by semiquantitative analysis of alizarin red staining. mRNA expression of osteogenic differentiation related genes was examined by real-time quantitative polymerase chain reaction (RT-qPCR).Statistical analysis was performed using SPSS 17.0 software package.Compared with the control group, 3,4 and 5 mmol/L CaLow Ca more...
- Published
- 2020
5. Self-assembled GeO
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Sheng-Chao, Song, Ding-Chuan, Zuo, Chang-Sheng, An, Xia-Hui, Zhang, Jin-Hui, Li, Zhen-Jiang, He, Yun-Jiao, Li, and Jun-Chao, Zheng
- Abstract
High-capacity germanium-based anode materials are alternative materials for outstanding electrochemical performance lithium-ion batteries (LIBs), but severe volume variation and pulverization problems during charging-discharging processes can seriously affect their electrochemical performance. In addressing this challenge, a simple strategy was used to prepare the self-assembled GeO more...
- Published
- 2020
6. Atorvastatin attenuates experimental contrast-induced acute kidney injury: a role for TLR4/MyD88 signaling pathway
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Rongzheng Yue, Baihai Su, Rui Zeng, Ye Tao, Chuan Zuo, Songmin Huang, and Jing Zeng
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Male ,renal failure ,Atorvastatin ,030232 urology & nephrology ,Contrast Media ,030204 cardiovascular system & hematology ,Kidney Function Tests ,Critical Care and Intensive Care Medicine ,Blood Urea Nitrogen ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,0302 clinical medicine ,Laboratory Study ,Receptor ,Blood urea nitrogen ,NF-kappa B ,Acute kidney injury ,atorvastatin ,General Medicine ,Acute Kidney Injury ,Kidney Tubules ,Nephrology ,Creatinine ,Signal transduction ,Signal Transduction ,medicine.drug ,signaling pathway ,medicine.medical_specialty ,03 medical and health sciences ,Internal medicine ,medicine ,Animals ,RNA, Messenger ,Contrast-induced acute kidney injury ,Dose-Response Relationship, Drug ,urogenital system ,business.industry ,medicine.disease ,Rats ,Toll-Like Receptor 4 ,Disease Models, Animal ,Endocrinology ,chemistry ,Apoptosis ,Myeloid Differentiation Factor 88 ,TLR4 ,Hydroxymethylglutaryl-CoA Reductase Inhibitors ,business ,TLR4/MyD88 - Abstract
Objectives: To investigate the protective effect of different atorvastatin doses on contrast-induced acute kidney injury and the related mechanism. Methods: Healthy male Sprague–Dawley (SD) rats were randomly divided into the blank control group, experimental control group and different-dose atorvastatin groups. A rat model of contrast-induced acute kidney injury was established. We detected changes in serum creatinine (Scr) and blood urea nitrogen (BUN) before and after model establishment, observed and scored renal tubular injury, analyzed rat renal cell apoptosis, and measure the expression of signal pathway proteins and downstream inflammatory factors. Results: After contrast agent injection, the Scr and BUN levels of the experimental control group were significantly increased, the different doses applied in the atorvastatin group significantly reduced the Scr and BUN levels (p more...
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- 2017
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7. Additional file 1: of Friend or Foe? Flipped Classroom for Undergraduate Electrocardiogram Learning: a Randomized Controlled Study
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Rui, Zeng, Lian-Rui, Xiang, Rong-Zheng, Yue, Jing, Zeng, Xue-Hong, Wan, and Chuan, Zuo
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ComputingMilieux_COMPUTERSANDEDUCATION - Abstract
Interview questionnaire on teacherâ s attitudes towards the two pedagogics. It is semi-structured interview questionnaire of teachers to understand their experiences of both the flipped classroom and LBL method. Twenty teachers who participated in the interviews talked about the time they spent and other learnings. In addition, they were asked to provide a subjective evaluation of the teaching atmosphere and the perceived effects of the respective teaching method they used. For data collection and analysis, all these information were also recorded using videos. (DOCX 20 kb) more...
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- 2017
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8. A Controlled Trial to Compare the Teaching Quality of Clinical-Skills Training Faculty: The Clinician-Educator Career Path in China
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Yiping Wang, Jing Zeng, and Chuan Zuo
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Adult ,Male ,China ,medicine.medical_specialty ,Faculty, Medical ,Students, Medical ,media_common.quotation_subject ,education ,Career path ,Physical examination ,Education ,law.invention ,Randomized controlled trial ,law ,Surveys and Questionnaires ,Clinician educator ,medicine ,Humans ,Medical history ,Quality (business) ,Schools, Medical ,media_common ,Medical education ,medicine.diagnostic_test ,business.industry ,Teaching ,Medical school ,General Medicine ,Middle Aged ,Career Mobility ,Family medicine ,Female ,Clinical Competence ,Educational Measurement ,business ,Clinical skills ,Education, Medical, Undergraduate - Abstract
Full-time clinician educators are becoming more predominant in China, yet their effect is unknown.The purpose of this study was to compare the teaching quality of full-time (FT) faculty with that of part-time (PT) faculty in one Chinese medical school.In 3 consecutive years, 881 3rd year medical students were enrolled and randomly distributed into two groups, being taught by either a FT faculty member or a PT faculty member. Their teaching quality was evaluated with student performance on a written exam, the standardized patient exam, and student satisfaction.The students in the FT group always scored better on the written exam (1st year = 79.82 ± 9.2 vs. 81.26 ± 8.2, p.188; 2nd year = 73.10 ± 9.8 vs. 76.51 ± 7.9, p = .001; 3rd year = 75.15 ± 9.0 vs. 79.51 ± 8.7, p.0001). In the standardized patient exam, the students from FT groups always showed better performance in history taking and physical examination. Students continually gave higher evaluations to FT faculty in questionnaires (1st year = 76.8 ± 6.5 vs. 84.3 ± 2.2; 2nd year = 78.6 ± 3.9 vs. 89.7 ± 4.2; 3rd year = 75.8 ± 3.9 vs. 88.5 ± 3.5, all ps.001).The teaching quality of FT faculty in clinical-skills training is better than PT faculty in this study. more...
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- 2014
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9. Treatment of Primary Chronic Glomerulonephritis with Rehmannia Glutinosa Acteosides in Combination with the Angiotensin Receptor Blocker Irbesartan: A Randomized Controlled Trial
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Fang Liu, Mei Shi, Ping Feng, Chuan Zuo, Ping Fu, Lina Cao, Peng Shi, Feng Chen, Hong-yu Qiu, Wen-Xing Fan, Li Zhou, YaPing Liang, Hui Zhong, and Zhong-ping Gou
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Pharmacology ,medicine.medical_specialty ,Angiotensin receptor ,Proteinuria ,biology ,business.industry ,Urology ,Aspartate transaminase ,Renal function ,urologic and male genital diseases ,Rehmannia glutinosa ,biology.organism_classification ,female genital diseases and pregnancy complications ,law.invention ,Irbesartan ,Randomized controlled trial ,law ,biology.protein ,Outpatient clinic ,Medicine ,medicine.symptom ,business ,medicine.drug - Abstract
This study aims to assess the efficacy and safety of Rehmannia glutinosa acteosides used in combination with the angiotensin receptor blocker irbesartan to treat primary chronic glomerulonephritis. A total of 479 patients diagnosed with primary chronic glomerulonephritis were recruited from outpatient clinics and were randomly assigned to the treatment group (Rehmannia glutinosa acteosides, two 200-mg capsules, bid; and irbesartan, one 150-mg tablet, qd) or the control group (irbesartan, one 150-mg tablet, qd). The primary outcome was 24-h urinary protein. Secondary outcome measures included blood pressure, estimated glomerular filtration rate, erythrocyturia, serum alanine aminotransferase, aspartate transaminase and electrolytes. After 8 weeks of treatment, the treatment group showed a mean reduction in 24-h proteinuria of 36.42% compared to baseline, which was significantly higher than the mean reduction from baseline of 27.97% in the control group (P = 0.0278).Adverse drug reactions occurred at a similarly low rate in the treatment group (0.4%) and control group (1.2%, P = 0.3724). In the treatment of chronic glomerulonephritis, the combination of Rehmannia glutinosa acteosides and irbesartan can reduce proteinuria more effectively than irbesartan alone. more...
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- 2013
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10. Regulation of the Pacemaker Activity of Colonic Interstitial Cells of Cajal by Protease-Activated Receptors: Involvement of Hyperpolarization-Activated Cyclic Nucleotide Channels
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Man Woo Kim, Dong Chuan Zuo, Jae Yeoul Jun, Ju Yeon Cho, Jun Lee, Young Dae Kim, Seok Choi, Chan Guk Park, Dong Hoon Shin, and Insuk So
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0301 basic medicine ,Male ,medicine.medical_specialty ,Thapsigargin ,Patch-Clamp Techniques ,Colon ,Receptors, Proteinase-Activated ,Biology ,03 medical and health sciences ,Cyclic nucleotide ,chemistry.chemical_compound ,symbols.namesake ,Pacemaker potential ,Mice ,Internal medicine ,medicine ,Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels ,Animals ,Receptor, PAR-2 ,Receptor, PAR-1 ,Patch clamp ,Pharmacology ,Mice, Inbred BALB C ,Phospholipase C ,Depolarization ,General Medicine ,Hyperpolarization (biology) ,Interstitial Cells of Cajal ,Interstitial cell of Cajal ,Cell biology ,030104 developmental biology ,Endocrinology ,chemistry ,symbols ,Female ,Oligopeptides - Abstract
Background and Purpose: The exact mechanism of protease-activated receptors (PARs) on pacemaker activity of interstitial cells of Cajal (ICCs) has not been reported. We investigated the effects on pacemaker activity by the activation of PARs and its signal mechanisms in colonic ICCs. Methods: The whole-cell patch-clamp technique, RT-PCR and Ca2+ imaging were used in cultured ICCs from mouse colon. Results: PAR-1 and PAR-2 were expressed in Ano-1 positive ICCs. TFLLR-NH2 (a PAR-1 agonist) and trypsin (a PAR-2 agonist) depolarized the membrane and increased the pacemaker potential frequency. U-73122 (a phospholipase C (PLC) inhibitor) and thapsigargin (a Ca2+ ATPase inhibitor) suppressed the TFLLR-NH2- and trypsin-induced effects on pacemaker potential. TFLLR-NH2 and trypsin also increased intracellular Ca2+ ([Ca2+]i) intensity with increasing of Ca2+ oscillations. Genistein (a tyrosine kinase inhibitor), SP600125 (a JNK inhibitor), CsCl, ZD7288, clonidine (hyperpolarization-activated cyclic nucleotide (HCN) channel blockers), SQ-22536 and dideoxyadenosine (adenylate cyclase inhibitors) suppressed the increased pacemaker potential frequency without effects on depolarization of the membrane induced by TFLLR-NH2 and trypsin. Conclusion: These results suggest that activation of PAR-1 and PAR-2 modulates the pacemaker activity of colonic ICCs through the PLC-dependent [Ca2+]i release pathway. The increased pacemaker potential frequency by PAR-1 and PAR-2 was also dependent on tyrosine kinase, JNK, and HCN activation. more...
- Published
- 2016
11. Ginsenoside Rg1 modulation on thrombospondin-1 and vascular endothelial growth factor expression in early renal fibrogenesis in unilateral obstruction
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Yao Deng, Chuan Zuo, Feng-Ping Wang, Jun-ming Fan, Heng-Chuan Liu, Cheng-Long Zhang, and Xi-Sheng Xie
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Pharmacology ,medicine.medical_specialty ,Kidney ,Endothelium ,urogenital system ,business.industry ,Angiogenesis ,urologic and male genital diseases ,medicine.disease ,Peritubular capillaries ,Vascular endothelial growth factor ,chemistry.chemical_compound ,Endocrinology ,medicine.anatomical_structure ,chemistry ,Fibrosis ,Internal medicine ,medicine ,Renal fibrosis ,business ,Nephritis - Abstract
Renal interstitial fibrosis is the major histopathological change seen in a variety of renal disorders and is closely related to renal dysfunction. Progressive interstitial fibrosis accompanied by the loss of renal tubules and interstitial capillaries typifies all progressive renal disease. Thrombospondin-1 (TSP-1) is a major angiogenic inhibitor. It is demonstrated that TSP-1 levels were correlated with the loss of glomerular and peritubular capillaries and TSP-1 could promote renal scarring by effects on the endothelium. It has been reported that ginsenoside Rg1 inhibited renal interstitial fibrosis in rats via suppressing the expression of TSP-1. The present study was designed to examine whether ginsenoside Rg1 could modulate the integrity of the microvasculature and hence affect the progression of renal fibrosis in a rat unilateral ureteral obstruction (UUO) model. In UUO control kidneys, associated with interstitial fibrosis, lower peritubular capillary densities were prominent. These changes were all improved by ginsenoside Rg1 treatment. Interestingly, ginsenoside Rg1 decreased the expression of TSP-1 and enhanced vascular endothelial growth factor (VEGF) expression. The results show for the first time that ginsenoside Rg1 can evidently inhibit renal interstitial fibrosis in rats with UUO. The mechanism might be related to suppression of the expression of TSP-1 and to repair of the peritubular capillary. more...
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- 2010
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12. Capsaicin Inhibits the Spontaneous Pacemaker Activity in Interstitial Cells of Cajal From the Small Intestine of Mouse
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Dong Chuan Zuo, Hyun Il Kim, Pawan K Shahi, Seok-Yong Choi, Jae Myeong Sun, and Jae Yeoul Jun
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Gastrointestinal motility ,business.industry ,Gastroenterology ,TRPV1 ,Pharmacology ,Bioinformatics ,TRPV ,Calcium in biology ,Interstitial cell of Cajal ,symbols.namesake ,chemistry.chemical_compound ,Transient receptor potential channel ,Pacemaker potential ,chemistry ,Capsaicin ,Interstitial cells of Cajal ,symbols ,Medicine ,Original Article ,Pacemaker current ,lipids (amino acids, peptides, and proteins) ,Neurology (clinical) ,business ,Capsazepine - Abstract
BACKGROUND/AIMS Capsaicin (8-methyl-N-vanillyl-6-ninenamide), a compound found in hot peppers, has been reported to have different physiological actions on different cell types. Not much work has been done about the effect of capsaicin on the function of interstitial cells of Cajal (ICC). In the present study, we examined the action of external application of capsaicin on pacemaker activity in the cultured ICC from the small intestine of mouse. METHODS We investigated the effect of capsaicin on pacemaker currents in cultured ICC from the small intestine of mouse using a whole cell patch-clamp technique and Ca(2+)-imaging analysis. RESULTS When capsaicin was applied externally to the pacemaker generating ICC, it completely inhibited the pacemaker potential under current-clamp mode (I = 0) and the pacemaker current under voltage-clamp mode at a -70 mV of holding potentials. The effect of capsaicin on pacemaker activity in ICC was shown dose dependently. The effect of capsaicin was not through the transient receptor potential of the vanilloid type 1 (TRPV1) channel as capsazepine did not block the effect of capsaicin. L-NAME, an inhibitor of nitric oxide synthase, also did not block the capsaicin-induced effects. When the action of capsaicin was examined in the intracellular calcium oscillation, it completely abolished the calcium oscillation. CONCLUSIONS These results prove that the capsaicin has the inhibitory effects on the ICC which is carried out neither through TRPV channel nor the nitric oxide production. Intracellular Ca(2+) was also an important target for actions of capsaicin on ICC. more...
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- 2010
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13. Astragalus mongholicus ameliorates renal fibrosis by modulating HGF and TGF-β in rats with unilateral ureteral obstruction
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Da Zhu, Yao Deng, Xi-sheng Xie, Hong-yu Qiu, Chuan Zuo, and Jun-ming Fan
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Male ,medicine.medical_specialty ,urologic and male genital diseases ,General Biochemistry, Genetics and Molecular Biology ,Rats, Sprague-Dawley ,Western blot ,Internal medicine ,medicine ,Renal fibrosis ,Animals ,General Pharmacology, Toxicology and Pharmaceutics ,Nephrosclerosis ,General Veterinary ,medicine.diagnostic_test ,biology ,business.industry ,Astragalus Plant ,General Medicine ,biology.organism_classification ,Rats ,Biomedicine ,Astragalus ,Treatment Outcome ,Endocrinology ,Losartan ,Hepatocyte growth factor ,business ,Myofibroblast ,Type I collagen ,Drugs, Chinese Herbal ,Ureteral Obstruction ,medicine.drug ,Transforming growth factor - Abstract
Astragalus mongholicus (AM) derived from the dry root of Astragalus membranaceus Bge. var. mongolicus (Bge.) Hsiao is a widely used traditional Chinese medicine. The present study investigated the potential role of AM on renal fibrosis on a rat model of unilateral ureteral obstruction (UUO). We divided 48 Sprague-Dawley rats randomly into 4 groups: sham-operated group (Sham), untreated UUO group, AM-treated (10 g/(kg x d)) UUO group, and losartan-treated (20 mg/(kg x d)) UUO group as positive control. Haematoxylineosin (HE) and Masson staining were used to study the dynamic histological changes of the kidneys 7 and 14 d after operation. The expressions of fibronectin (FN), type I collagen (colI), hepatocyte growth factor (HGF), transforming growth factor-beta1 (TGF-beta1), and alpha-smooth muscle actin (alpha-SMA) were analyzed by real-time polymerase chain reaction (PCR), immunohistochemistry staining, and Western blot. Results show that, similar to losartan, AM alleviated the renal damage and decreased the deposition of FN and colI from UUO by reducing the expressions of TGF-beta1 and alpha-SMA (P0.05), whereas HGF increased greatly with AM treatment (P0.05). Our findings reveal that AM could retard the progression of renal fibrosis. The renoprotective effect of AM might be related to inhibition of myofibroblast activation, inducing of HGF and reducing of TGF-beta1 expression. more...
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- 2009
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14. Ginsenoside Rb1, a panoxadiol saponin against oxidative damage and renal interstitial fibrosis in rats with unilateral ureteral obstruction
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Yao Deng, Jun-ming Fan, Man Yang, Heng-Chuan Liu, Chuan Zuo, and Xi-sheng Xie
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Male ,medicine.medical_specialty ,Ginsenosides ,Drug Evaluation, Preclinical ,Saponin ,Urology ,Kidney ,urologic and male genital diseases ,medicine.disease_cause ,Renal interstitial fibrosis ,Models, Biological ,Rats, Sprague-Dawley ,Transforming Growth Factor beta1 ,Oxidative damage ,Fibrosis ,Internal medicine ,Animals ,Medicine ,Pharmacology (medical) ,chemistry.chemical_classification ,urogenital system ,business.industry ,Deoxyguanosine ,NADPH Oxidases ,8-Hydroxy-2'-deoxyguanosine ,Kidney metabolism ,General Medicine ,Saponins ,medicine.disease ,eye diseases ,female genital diseases and pregnancy complications ,Rats ,Oxidative Stress ,Endocrinology ,Gene Expression Regulation ,Complementary and alternative medicine ,chemistry ,8-Hydroxy-2'-Deoxyguanosine ,Ginsenoside Rb1 ,Heme Oxygenase (Decyclizing) ,Kidney Diseases ,business ,Oxidative stress ,Ureteral Obstruction - Abstract
To investigate the possible protective effect and mechanism of ginsenoside Rb1 against oxidative damage and renal interstitial fibrosis on rats with unilateral ureteral obstruction (UUO).In total, 80 male rats were randomly divided into 4 groups, 20 in each group: the sham operated group (SOR), UUO group, UUO with ginsenoside Rb1 treatment group (treated with intraperitoneal injection of 50 mg/ kg daily) and UUO with Losartan treatment group (as the positive control, treated with 20 mg/kg by gastrogavage per day). The rats were randomly sacrificed on day 3, 7 and 14 after surgery, respectively. The histopathologic changes of renal interstitial tissues were observed with Masson staining. The mRNA of transforming growth factor beta 1 (TGF-beta 1), collagen I and fibronectin were reversed transcribed and quantified by Real-time PCR. Enzyme-linked immunosorbent assay was used to quantitatively detect TGF-beta 1 and 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels. P47phox protein expression was assessed by immunohistochemistry and Western blot analysis.In the UUO model, the obstructed kidney showed typical features of progressive renal tubulointerstitial fibrosis, and the levels of TGF-beta1, collagen I and fibronectin increased (P0.05). As compared with the UUO group, ginsennoside Rb1 significantly inhibited the interstitial fibrosis including tubular injury and collagen deposition, and decreased the levels of TGF-beta1 (P0.05). Ginsenoside Rb1 also inhibited the heme oxygenase (HO-1) and 8-OHdG, two markers of oxidative stress (P0.05). Moreover, ginsenoside Rb1 suppressed the expression of p47phox, a subunit of nicotinamide adeninedinucleotide phosphate (NADPH) oxidase (P0.05).Ginsenoside Rb1 can obviously inhibit renal interstitial fibrosis in rats with UUO, its mechanism possibly via against the oxidative damage and suppressing TGF-beta1 expression. more...
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- 2009
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15. [Controlled clinical study on compound Decumbent Corydalis Rhizome and diclofenac in treatment of knee osteoarthritis]
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Chuan, Zuo, Geng, Yin, Xiao-Min, Cen, and Qi-Bing, Xie
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Adult ,Male ,Diclofenac ,Treatment Outcome ,Corydalis ,Humans ,Female ,Middle Aged ,Osteoarthritis, Knee ,Rhizome ,Aged ,Drugs, Chinese Herbal - Abstract
To evaluate the efficacy and safety of compound Decumbent Corydalis Rhizome (DCR) in treating patients with knee osteoarthritis (OA). Totally 79 patients with knee osteoarthritis were selected from out-patient and inpatient departments of West China Hospital and randomly divided into the test group and the control group. The test group (n = 41) was given Compound DCR with the dosage of 1.8 g · d(-1), while the control group (n = 38) was administered with diclofenac sodium with the dosage of 75 mg · d(-1). After 12 weeks of treatment, the total efficacy rates based on patients/physicians evaluation for experimental and control groups were 68.29%, 63.41% and 71.05%, 63.16%, respectively, without significant difference between the two groups. Both of the two groups showed significant improvements in the main efficacy indexes (pain on walking 20 m) and minor indexes (tenderness on palpation, Western Ontario and McMaster Universities OA index (WOMAC) and Short-Form Health Survey (SF-36 ), but without significant difference in efficacy between them. The incidence of related adverse events was 24.39% in the test group and 47.37% in the control group, respectively, with significant differences between the two groups (P0.05). In the controlled study, compound DCR is as efficient as diclofenac sodium but more tolerable, with a good clinical application prospect. more...
- Published
- 2015
16. SP157INVESTIGATION OF THE PROTECTIVE EFFECTS OF ATORVASTATION ON CONTRAST-INDUCED ACUTE KIDNEY INJURY FROM THE PERSPECTIVE OF THE TLRA/MYD88 SIGNALING PATHWAY
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Baihai Su, Songmin Huang, Jing Zeng, Rui Zeng, Rongzheng Yue, Ye Tao, and Chuan Zuo
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Transplantation ,business.industry ,media_common.quotation_subject ,Perspective (graphical) ,Acute kidney injury ,medicine.disease ,Signal pathway ,Nephrology ,medicine ,Contrast (vision) ,Signal transduction ,business ,Neuroscience ,media_common - Published
- 2017
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17. Targeting mTOR/p70S6K/glycolysis signaling pathway restores glucocorticoid sensitivity to 4E-BP1 null Burkitt Lymphoma
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Yanle Zhang, Yiping Zhu, Ju Gao, Ling Gu, Chuan Zuo, Liping Xie, and Zhigui Ma
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Cancer Research ,medicine.medical_specialty ,Resistance ,Antineoplastic Agents ,Apoptosis ,Deoxyglucose ,environment and public health ,Dexamethasone ,Mice ,Glucocorticoid Sensitivity ,Glucocorticoid ,Internal medicine ,Cell Line, Tumor ,Antineoplastic Combined Chemotherapy Protocols ,Genetics ,medicine ,Animals ,Humans ,Rapamycin ,Glucocorticoids ,PI3K/AKT/mTOR pathway ,Sirolimus ,Mammalian target of rapamycin ,business.industry ,fungi ,Autophagy ,RPTOR ,Cell Cycle ,Ribosomal Protein S6 Kinases, 70-kDa ,Burkitt lymphoma ,Drug Synergism ,Xenograft Model Antitumor Assays ,Raji ,enzymes and coenzymes (carbohydrates) ,Endocrinology ,Oncology ,Drug Resistance, Neoplasm ,Cancer research ,Phosphorylation ,Signal transduction ,p70S6 kinase ,business ,Glycolysis ,medicine.drug ,Signal Transduction ,Research Article - Abstract
Background Increasing evidence indicates that rapamycin could be used as a potential glucocorticoid (GC) sensitizer in lymphoblastic malignancies via genetic prevention of 4E-BP1 phosphorylation. Interestingly, we found that combined rapamycin with dexamethasone can effectively reverse GC resistance in 4E-BP1 null lymphoma cells. In this study, we investigated the potential link between mTOR/p70S6K signaling pathway, glycolysis, autophagy and GC resistance. Methods Antitumor effects of the combination of rapamycin and dexamethasone were evaluated on cell viability by MTT assay and in vivo studies, on cell cycle and apoptosis by flow cytometry, on autophagy by western blot, MDC staining and transmission electron microscopy and on cell signaling by western blot. Moreover, to test whether inhibiting glycolysis is the core mechanism in rapamycin restoring GC sensitivity, we took glycolysis inhibitor 2-deoxyglucose to replace rapamycin and then evaluated the antitumor effects in vitro. Results Raji cells are resistant to rapamycin (IC50 > 1000 nM) or dexamethasone (IC50 > 100 μM) treatment alone. The combination of rapamycin and dexamethasone synergistically inhibited the viability of Raji cells in vitro and in vivo by inducing caspase-dependent and -independent cell death and G0/G1 cell cycle arrest. These effects were achieved by the inhibition of mTOR/p70S6K signaling pathway, which led to the inhibition of glycolysis and the induction of autophagy. Pretreatment with pan-caspase inhibitor z-VAD-fmk or autophagy inhibitor 3-MA failed to protect the cells from combined treatment-induced death. Glycolysis inhibitor combined with dexamethasone produced a similar antitumor effects in vitro. Conclusions Inhibition of mTOR/p70S6K/glycolysis signaling pathway is the key point of therapy in reversing GC resistant in Burkitt lymphoma patients. Electronic supplementary material The online version of this article (doi:10.1186/s12885-015-1535-z) contains supplementary material, which is available to authorized users. more...
- Published
- 2014
18. [Endogenous hydrogen sulfide and androgen deficiency-induced erectile dysfunction in rats]
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Chuan, Zuo, Yi-Ming, Huang, Rui, Jiang, Hai-Fan, Yang, Bo, Cheng, and Feng, Chen
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Male ,Rats, Sprague-Dawley ,Erectile Dysfunction ,Androgens ,Cystathionine gamma-Lyase ,Animals ,Cystathionine beta-Synthase ,Hydrogen Sulfide ,Orchiectomy ,Penis ,Rats - Abstract
To investigate the role of endogenous hydrogen sulfide (H2S) in erectile dysfunction (ED) induced by androgen deficiency.We randomly divided 30 eight-week-old healthy male SD rats into six groups: 2-week control (A), 4-week control (B), 2-week castration (C), 4-week castration (D), 2-week castration + androgen replacement (E), and 4-week castration + androgen replacement (F), those in groups E and F subcutaneously injected with testosterone propionate (TP) at the physiological dose of 3 mg/kg per day after castration, while those in the other groups with isodose oil instead. At 2 and 4 weeks after operation, we determined the level of serum testosterone (T) , intracavernous pressure (ICP) , mean carotid arterial pressure (MAP) of the rats, measured the concentration of H2S in the plasma and corpus cavernosum tissue, and detected the expressions of cystathionine-P3-synthase (CBS) and cystathionine-gamma-lyase (CSE) by immunohistochemistry and Western blot.The serum T level was significantly lower in group C ([0.63 +/- 0.15] nmol/L) than in A ( [ 16.55 +/- 4.17] nmol/L) and E ( [ 18.99 +/- 4.62] nmol/L) (P0.05), as well as in group D ([0.70 +/-0.22] nmol/L) than in B ([15.44 +/-5.18] nmol/L) and F ([20.99 +/-6.41] nmol/L) (P0. 05) , and so were ICP/MAP after 5 and 7 V electrical stimulation of the pelvic ganglia (P0. 05) , H2 S concentration (P0.05), and the expressions of CBS and CSE (P0.05). The expressions of CBS and CSE proteins were also significantly decreased in group C as compared with D (P0.05).The reduced expressions of CBS and CSE may inhibit the H2 S signaling pathway, which might be one of the mechanisms underlying androgen deficiency-induced ED in rats. more...
- Published
- 2014
19. [Expression of Gphaq in peripheral blood T lymphocytes of patients with polymyositis and its correlation with disease activity]
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Min, Yang, Yan, Liang, Yan, He, Chuan, Zuo, Geng, Yin, and Qibing, Xie
- Subjects
Adult ,Male ,Young Adult ,Case-Control Studies ,T-Lymphocytes ,GTP-Binding Protein alpha Subunits, Gq-G11 ,Humans ,Female ,RNA, Messenger ,Middle Aged ,Aged ,Polymyositis ,Receptors, G-Protein-Coupled - Abstract
To analyze the expression of Gaq in peripheral blood T lymphocytes of the patients with polymyositis (PM) and its correlation with disease activity evaluated by the clinical markers [manual muscle test (MMT),myositis disease activity assessment (MDAA),creatine phosphokinase (CK), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP)].Blood samples were obtained from 30 patients with first onset PM and 30 healthy volunteers. Peripheral blood mononuclear cells (PBMCs) from these patients were collected and CD3+ T cells from PBMCs were sorted out with magnetic beans. The mRNA and protein expression levels of Galphaq in T cells were measured by RT-PCR and Western blot. Student's unpaired 2-tailed t-test was applied to identify Galphaq expression difference between PM patients and healthy controls. Pearson's correlation between Galphaq mRNA expression of PM and clinical evaluation markers MMT, MDAA, CK, ESR or CRP was determined.Compared to health control, PM patients had significantly lower expression levels of Galphaq protein and mRNA in peripheral blood CD3+ T cells (P0. 001). Negative correlation was found between Galphaq mRNA level and MMT (r= -0. 704, P0. 001), MDAA (r= -0. 793, P0. 001) or CK (r= -0. 670, P0. 001), and no correlation was found between Galphaq mRNA level and ESR or CRP.Galphaq expression in T cells of PM is lower than that in normal control and has negative correlation with PM disease activity. more...
- Published
- 2013
20. [Survival analysis and risk factors for survival in idiopathic inflammatory myopathies: a retrospective cohort study]
- Author
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Geng, Yin, Yan, Liang, Rong, Deng, Ying, Wang, Qi-Bing, Xie, and Chuan, Zuo
- Subjects
Adult ,Male ,Myositis ,Cardiovascular Diseases ,Risk Factors ,Neoplasms ,Humans ,Female ,Kaplan-Meier Estimate ,Middle Aged ,Survival Analysis ,Proportional Hazards Models ,Retrospective Studies - Abstract
To study predominant risk factors for survival in idiopathic inflammatory myopathies (JIM).The clinical data of 163 cases of IIM were reviewed, who received the diagnosis and treatments in West China Hospital from January 1997 to December 2010. Kaplan-Meier survival curve was used for the survival analysis of these patients with IIM. Univariate and multivariate analysis of risk factors for survival in the patients with IIM were carried out by Log-rank test, univariate Cox regression analysis and Cox regression model respectively.There were 21 death occured among 163 patients with IIM, and the estimated 3, 5, 10-year survival rate was 93%, 89%, 80% respectively. The mortality of the patients with dermatomyositis (DM) or amyophathic dermatomyositis(ADM) increased significantly when compared with polymyositis (PM, P=0. 033, P= 0. 06). The mortality of IIM patients complicated with tumor or cadiovascular involvement was much higher than that of those patients without any complications (P0. 001, P=0. 015). Tumor and cadiovascular involvement were two independent risk factors for the survival of IIM individuals.DM and ADM have much unfavorable prognosis than PM. Tumor and cadiovascular involvement are two major risk factors for the survival of IIM patients. more...
- Published
- 2013
21. [Risk factors associated with cardiac involvement in patients with dermatomyositis/polymyositis]
- Author
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Chuan, Zuo, Xuan-Di, Wei, Ya-Li, Ye, Yan, Liang, Geng, Yin, Min, Yang, and Qi-Bing, Xie
- Subjects
Adult ,Male ,Adolescent ,Heart Diseases ,Age Factors ,Myocardial Infarction ,Middle Aged ,Dermatomyositis ,Polymyositis ,Young Adult ,Risk Factors ,Humans ,Female ,Lung Diseases, Interstitial ,Aged ,Retrospective Studies - Abstract
To identify risk factors associated with cardiac involvement (CI) in patients with dermatomyositis/polymyositis (DM/PM).Medical records of 129 DM/PM patients were reviewed retrospectively. The risk factors associated with CI in those patients were screened through Xt tests or independent tests before a multivariate logistic regression analysis was performed. ROC curves were constructed to determine diagnostic values of the identified risk factors.CI occurred in 59 (45. 74%) of DM/PM patients, with 41 (69. 49%) showing electrocardiographic (ECG) abnormality; 25 (42. 37%) showing ultrasonic cardiogram (UCG) abnormality; 8 (13. 56%) being diagnosed with heart failure, and 2 (3. 39%) being diagnosed with myocardial infarction. Eight (13.56%) of the patients with CI died. CI was more likely to occur in patients with an older age, having interstitial lung disease, antinuclear antibody (ANA) positive, and anti-Jo-1 antibody positive (P0.05). The logistic regression analysis revealed that interstitial lung disease (beta=1. 554), aspartic aminotransferase/creatine kinases (AST/CK) ratio (beta=1.189), positive ANA (beta= 1. 172) and age (beta=0.042) were risk factors associated with CI (P0. 05). Notable areas under ROC curve (0. 642) was found for AST/CK in determining CI in DM/PM patients (P0. 05), albeit with low accuracy. A cut-off of AST/CK ratio at 0. 312 was identified as a reference point for determining CI in patients with DM/PM.Cardiac involvement is the most common complication of DM/PM, although the majority are subclinical. ECG and UCG are common tools for diagnosing cardiac involvement. Interstitial lung disease, AST/CK ratio, positive ANA and age are predictors of CI in DM/PM patients. more...
- Published
- 2013
22. [Idiopathic inflammatory myopathies complicated with pneumonias: clinical characteristics and pathogen analysis]
- Author
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Yan, Liang, Rong, Deng, Xin, Guo, Chuan, Zuo, Qi-Bing, Xie, and Geng, Yin
- Subjects
Adult ,Male ,Adolescent ,Myositis ,Age Factors ,Pneumonia ,Middle Aged ,Klebsiella pneumoniae ,Young Adult ,Logistic Models ,Risk Factors ,Candida albicans ,Gram-Negative Bacteria ,Humans ,Female ,Lung Diseases, Interstitial ,Aged - Abstract
To investigate the clinical characteristics, pathogen constitution and their tolerance to antibiotics in idiopathic inflammatory myopathies (IIM) patients complicated with pneumonia and the associated risk factors.The clinical data and pathogen test results of 93 IIM patients with pneumonia and 52 IIM patients without pneumonia (control group) were retrieved and compared.Age of onset and interstitial lung disease were identified as risk factors associated with IIM complicated with pneumonia. Gram negative bacteria were the most common pathogen. Klebsiella pneumoniae was the most common bacteria and Candida albicans was the most common fungus causing infections in the IIM patients, which were relatively sensitive to the third- and fourth-generation of antibiotics such as cephalosporin, fluoroquinolones and aminoglycoside.Older patients with interstitial lung disease are prone to having pneumonia, especially those caused by gram negative bacteria and other atypical pathogens. Timely and reasonable anti-infection treatment is essential. more...
- Published
- 2013
23. [Comparison of four diagnostic criteria for idiopathic inflammatory myopathy]
- Author
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Xiao-Min, Cen, Ya-Fei, Cai, Ling, Ma, Qi-Bing, Xie, Geng, Yin, and Chuan, Zuo
- Subjects
Adult ,Male ,Myositis ,ROC Curve ,Electromyography ,Humans ,Female ,Middle Aged ,Reference Standards ,Sensitivity and Specificity ,Dermatomyositis ,Retrospective Studies - Abstract
To compare the clinical values of four different criteria for diagnosing idiopathic inflammatory myopathy (IIM).The four different criteria published in 1975, 1995, 1997 and 2004 were applied to 94 IIM patients and 98 patients with other myopathies in West China Hospital.The sensitivity of the four criteria for diagnosing IIM was 56. 4% (1975), 87. 2% (1995), 61.7 % (1997), and 52. 1% (2004) respectively. The specificity of the four criteria for diagnosing IIM was 78. 6% (1975), 20. 4% (1995), 78. 6% (1997), and 90. 8% (2004) respectively. The Youden index was 35.0% (1975), 7.6% (1995), 40. 3% (1997), and 42.9% (2004), with an odd product of 4. 74 (1975), 1. 75 (1995), 5. 91 (1997) and 10. 77 (2004) respectively. The Kappa value of the four criteria was 0. 351 (1975, P0.05), 0. 075 (1995, P0. 05), 0. 404 (1997, P0.05 ) and 0. 433 (2004, P0.05), and their area under the ROC curve was 0. 675 (1975, P=0. 00), 0. 538 (1995, P=0.36),0. 701 (1997, P=0.00) and 0. 715 (2004, P=0.00) respectively.The 2004 criteria have a better value in diagnosing IIM. more...
- Published
- 2013
24. A clinical analysis of risk factors for interstitial lung disease in patients with idiopathic inflammatory myopathy
- Author
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Chuan Zuo, Qi-Bing Xie, Geng Yin, Xiao-Min Cen, and Min Yang
- Subjects
lcsh:Immunologic diseases. Allergy ,Adult ,Male ,medicine.medical_specialty ,Article Subject ,Adolescent ,Immunology ,Arthritis ,Logistic regression ,Gastroenterology ,Young Adult ,Risk Factors ,Internal medicine ,medicine ,Odds Ratio ,Immunology and Allergy ,Humans ,Young adult ,Myositis ,Aged ,Aged, 80 and over ,Clinical pathology ,business.industry ,Interstitial lung disease ,General Medicine ,Odds ratio ,Middle Aged ,medicine.disease ,Rheumatology ,Surgery ,body regions ,Clinical Study ,Female ,business ,lcsh:RC581-607 ,Lung Diseases, Interstitial - Abstract
Interstitial lung disease (ILD) is a common and severe complication of idiopathic inflammatory myopathies (IIM). The aim of our study was to identify risk factors for ILD by evaluating both clinical and biochemical features in IIM patients with or without ILD. From January 2008 to December 2011, medical records of 134 IIM patients in our rheumatology unit were reviewed. The patients were divided into ILD group (83 patients) and non-ILD group (51 patients). The clinical features and laboratory findings were compared. The univariable analyses indicated that arthritis/arthralgia (54.2% versus 17.6%,P<0.05), Mechanic’s hand (16.9% versus 2.0%,P<0.05), Raynaud’s phenomenon (36.1% versus 2.0%,P<0.05), heliotrope rash (44.6% versus 19.6%,P<0.05), fever (43.4% versus 21.6%,P<0.05), elevated ESR (60.2% versus 35.3%,P<0.05), elevated CRP (55.4% versus 31.4%,P<0.05), or anti-Jo-1 antibody (20.5% versus 5.9%,P<0.05) were risk factors for developing ILD in IIM. Multivariable unconditional logistic regression analysis that showed arthritis/arthralgia (OR 7.1, 95% CI 2.8–18.1), Raynaud’s phenomenon (OR 29.1, 95% CI 3.6–233.7), and amyopathic dermatomyositis (ADM) (OR 20.2, 95% CI 2.4–171.2) were the independent risk factors for developing ILD in IIM. more...
- Published
- 2013
25. Treatment of primary chronic glomerulonephritis with Rehmannia glutinosa acteosides in combination with the angiotensin receptor blocker irbesartan: a randomized controlled trial
- Author
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HongYu, Qiu, Ping, Fu, WenXing, Fan, Chuan, Zuo, Ping, Feng, Peng, Shi, Lina, Cao, Fang, Liu, Li, Zhou, Feng, Chen, Hui, Zhong, ZhongPing, Gou, YaPing, Liang, and Mei, Shi
- Subjects
Adult ,Male ,Biphenyl Compounds ,Tetrazoles ,Blood Pressure ,Irbesartan ,Middle Aged ,Rehmannia ,Proteinuria ,Glomerulonephritis ,Glucosides ,Phenols ,Humans ,Female ,Angiotensin II Type 1 Receptor Blockers - Abstract
This study aims to assess the efficacy and safety of Rehmannia glutinosa acteosides used in combination with the angiotensin receptor blocker irbesartan to treat primary chronic glomerulonephritis. A total of 479 patients diagnosed with primary chronic glomerulonephritis were recruited from outpatient clinics and were randomly assigned to the treatment group (Rehmannia glutinosa acteosides, two 200-mg capsules, bid; and irbesartan, one 150-mg tablet, qd) or the control group (irbesartan, one 150-mg tablet, qd). The primary outcome was 24-h urinary protein. Secondary outcome measures included blood pressure, estimated glomerular filtration rate, erythrocyturia, serum alanine aminotransferase, aspartate transaminase and electrolytes. After 8 weeks of treatment, the treatment group showed a mean reduction in 24-h proteinuria of 36.42% compared to baseline, which was significantly higher than the mean reduction from baseline of 27.97% in the control group (P = 0.0278).Adverse drug reactions occurred at a similarly low rate in the treatment group (0.4%) and control group (1.2%, P = 0.3724). In the treatment of chronic glomerulonephritis, the combination of Rehmannia glutinosa acteosides and irbesartan can reduce proteinuria more effectively than irbesartan alone. more...
- Published
- 2012
26. Effects of sphingosine-1-phosphate on pacemaker activity of interstitial cells of Cajal from mouse small intestine
- Author
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Seok Choi, Young Dae Kim, Jae Yeoul Jun, Jun Lee, Dong Chuan Zuo, Chan Guk Park, Pawan K Shahi, Man Yoo Kim, and Kyoung Taek Han
- Subjects
Agonist ,Male ,medicine.medical_specialty ,Thapsigargin ,Patch-Clamp Techniques ,medicine.drug_class ,SB 203580 ,Pyridines ,Suramin ,Biology ,Naphthalenes ,Ion Channels ,Membrane Potentials ,chemistry.chemical_compound ,symbols.namesake ,Mice ,Sphingosine ,Internal medicine ,Intestine, Small ,medicine ,Animals ,Patch clamp ,Enzyme Inhibitors ,Molecular Biology ,Sphingosine-1-Phosphate Receptors ,Cells, Cultured ,Mice, Inbred BALB C ,Endoplasmic reticulum ,Antinematodal Agents ,Cell Biology ,General Medicine ,Articles ,Interstitial Cells of Cajal ,Organophosphates ,Interstitial cell of Cajal ,Cell biology ,Receptors, Lysosphingolipid ,Calphostin C ,Endocrinology ,chemistry ,symbols ,Pyrazoles ,lipids (amino acids, peptides, and proteins) ,Calcium ,Female ,Lysophospholipids ,Mitogen-Activated Protein Kinases ,Signal Transduction - Abstract
Interstitial cells of Cajal (ICC) are the pacemaker cells that generate the rhythmic oscillation responsible for the production of slow waves in gastrointestinal smooth muscle. Spingolipids are known to present in digestive system and are responsible for multiple important physiological and pathological processes. In this study, we are interested in the action of sphingosine 1-phosphate (S1P) on ICC. S1P depolarized the membrane and increased tonic inward pacemaker currents. FTY720 phosphate (FTY720P, an S1P(1,3,4,5) agonist) and SEW 2871 (an S1P(1) agonist) had no effects on pacemaker activity. Suramin (an S1P(3) antagonist) did not block the S1P-induced action on pacemaker currents. However, JTE-013 (an S1P(2) antagonist) blocked the S1P-induced action. RT-PCR revealed the presence of the S1P(2) in ICC. Calphostin C (a protein kinase C inhibitor), NS-398 (a cyclooxygenase-2 inhibitor), PD 98059 (a p42/44 inhibitor), or SB 203580 (a p38 inhibitor) had no effects on S1P-induced action. However, c-jun NH(2)-terminal kinase (JNK) inhibitor II suppressed S1P-induced action. External Ca(2+)-free solution or thapsigargin (a Ca(2+)-ATPase inhibitor of endoplasmic reticulum) suppressed action of S1P on ICC. In recording of intracellular Ca(2+) ([Ca(2+)](i)) concentration using fluo-4/AM S1P increased intensity of spontaneous [Ca(2+)](i) oscillations in ICC. These results suggest that S1P can modulate pacemaker activity of ICC through S1P(2) via regulation of external and internal Ca(2+) and mitogenactivated protein kinase activation. more...
- Published
- 2012
27. [Comparison of the effects of alpha-keto/ amino acid supplemented low protein diet and diabetes diet in patients with diabetic nephropathy]
- Author
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Hong-yu, Qiu, Fang, Liu, Li-jun, Zhao, Song-min, Huang, Chuan, Zuo, Hui, Zhong, and Feng, Chen
- Subjects
Male ,Middle Aged ,Keto Acids ,Proteinuria ,Diabetes Mellitus, Type 2 ,Diet, Diabetic ,Dietary Supplements ,Diet, Protein-Restricted ,Humans ,Diabetic Nephropathies ,Female ,Prospective Studies ,Amino Acids ,Aged - Abstract
To investigate if a-keto/amino acid supplemented low protein diet can slow down the progression of diabetic nephrophathy in comparison with non-supplemented diabetes diet.A prospective, randomized, controlled clinical study was conducted. Twenty three cases of type 2 diabetic nephropathy in IV stage were randomly divided into alpha-keto/amino acid supplemented diet group (trial group) and conventional diabetes diet group (control group), The treatment duration was 52 weeks. 24 h urine protein was measured at 0, 12, 20, 36 and 52 weeks. Before and after the 52 weeks treatment, all the patients received the measurement of glomerular filtration rate (GFR), blood glucose, blood lipids, inflammatory markers, as well as nutritional status.After the treatment for 20, 36, 52 weeks, mean 24 h urine protein decreased significantly in trial groups (P0.05), and 24 h urine protein in trial group were significantly decreased (P0.05) compared with control group in 20 weeks after treatment. Either in trial group or in control group, GFR remained relatively stable during the observation period. Nutrition status, inflammatory markers, and serum calcium, phosphorus levels between the two groups were no significantly difference. The adverse events experienced by the patients in trial group were similar and consistent with the patients underlying renal diseases.Alpha-keto/amino acid can reduce proteinuria more effectively, while improve renal function and nutritional status in diabetic nephropathy patients with well-toleration. more...
- Published
- 2012
28. [Investigate the effects of compound radix notoginseng on renal interstitial fibrosis and kidney-targeting treatment]
- Author
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Xi-Sheng, Xie, Chuan, Zuo, Zi-Yuan, Zhang, Heng-Chuan, Liu, Sheng-Gang, Feng, Cheng-Long, Zhang, Wei, Yuan, and Jun-Ming, Fan
- Subjects
Male ,Panax notoginseng ,Kidney ,Fibrosis ,Actins ,Collagen Type I ,Losartan ,Fibronectins ,Rats ,Rats, Sprague-Dawley ,Random Allocation ,Animals ,Nephritis, Interstitial ,RNA, Messenger ,Drugs, Chinese Herbal ,Phytotherapy ,Ureteral Obstruction - Abstract
Investigate the effects of compound Radix Notoginseng on renal interstitial fibrosis and kidney-targeting treatment.100 healthy Sprague-Dawley rats were randomly divided into 5 groups: Unilateral ureteral obstruction (UUO) group, sham-operation (SOR) group, Radix Notoginseng (RN) group, compound Radix Notoginseng (CRN) group and Losartan (ARB) group. After operation, RN, CRN and ARB groups were intragastric administrated with RN (3 mL/d), CRN (3 mL/d) and ARB [20 mg/(kg x d)] respectively. Each group randomly included 18 rats for statistical analysis. The histological changes of renal interstitial tissues were observed by HE, Masson and PAS staining. Total kidney collagen content was determined by measuring the amount of hydroxyproline. The mRNA of alpha-SMA, collagen I and fibronectin were reverse transcribed and quantified by real-time PCR. The expression of alpha-SMA protein was assessed by immunohistochemistry and Western blot analysis.In UUO model, the obstructed kidney showed typical features of renal tubulointerstitial fibrosis, such as severe tubular loss, dilation, atrophy, infiltration of inflammatory cells, interstitial matrix deposition (P0.05). Partial correlation assay showed that the expression of alpha-SMA was related to the renal tubular injury (r = 0.55; P0.05). Administration of RN, CRN and ARB improved tubulointerstitial damage and collagen matrix accumulation induced by UUO in different degree. The expression of the alpha-SMA at mRNA and protein levels were significantly increased in the UUO group (P0.05), which was also suppressed by treatment with RN, CRN and ARB in different degree. Moreover, more effective role in preventing fibrosis was observed in CRN group than when compared with that of RN group.RN and CRN can inhibit UUO-induced renal interstitial fibrosis in rats, and CRN treatment is more effective than RN in reducing interstitial fibrosis. more...
- Published
- 2012
29. Action of lipopolysaccharide on interstitial cells of cajal from mouse small intestine
- Author
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Dong Chuan Zuo, Hak Sun Lee, Chan Guk Park, Jun Lee, Seok Choi, Seung Yong Seong, Hey Jung Moon, Su Cheong Yeom, Man Yoo Kim, Insuk So, Young Dae Kim, In Yeoup Chang, Jae Yeoul Jun, and Pawan K Shahi
- Subjects
Pharmacology ,Lipopolysaccharides ,medicine.medical_specialty ,Lipopolysaccharide ,Mouse Small Intestine ,General Medicine ,Protein-Tyrosine Kinases ,Interstitial Cells of Cajal ,Nitric Oxide ,Dinoprostone ,Interstitial cell of Cajal ,Cell biology ,Mice, Inbred C57BL ,Toll-Like Receptor 4 ,chemistry.chemical_compound ,symbols.namesake ,Mice ,Endocrinology ,chemistry ,Smooth muscle ,KATP Channels ,Internal medicine ,symbols ,medicine ,Animals - Abstract
Lipopolysaccharide (LPS) induces intestinal dysmotility by alteration of smooth muscle and enteric neuronal activities. However, there is no report on the modulatory effects of LPS on the interstitial cells of Cajal (ICCs). We investigated the effect of LPS and its signal transduction in ICCs.We performed whole-cell patch clamp and RT-PCR in cultured ICCs from mouse small intestine.LPS suppressed the generation of pacemaker currents of ICCs. The mRNA transcripts for Toll-like receptor 4 (TLR4) were expressed in ICCs. However, the inhibitory action of LPS on pacemaker currents from TLR4(+/+) mice was not present in TLR4(-/-) mice. The inhibitory effects of LPS on ICCs were blocked by glibenclamide (an inhibitor of ATP-sensitive K(+) channels), NS-398 (a COX-2 inhibitor), AH6808 [a prostaglandin E(2) (PGE(2))-EP(2) receptor antagonist], ODQ (an inhibitor of guanylate cyclase) and L-NAME [an inhibitor of nitric oxide synthase (NOS)]. Furthermore, genistein and herbimycin A (tyrosine kinase inhibitors) blocked the LPS-induced inhibitory action on pacemaker activity in ICCs.LPS can activate ICCs to release NO and PGE(2) through TLR4 activation. The released NO and PGE(2) inhibit pacemaker currents by activating ATP-sensitive K(+) channels. The LPS actions are mediated by tyrosine kinase signaling pathways. more...
- Published
- 2012
30. [Influence of mannose binding lectin to expression of TGF-beta1 and NF-kappaB in high concentration of glucose cultured human renal glomerular endothelial cells]
- Author
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Hong-Yu, Qiu, Wen-Xing, Fan, Chuan, Zuo, Song-Min, Huang, Fang, Liu, and Wan-Xin, Tang
- Subjects
Transforming Growth Factor beta1 ,Glucose ,Kidney Glomerulus ,NF-kappa B ,Endothelial Cells ,Humans ,Complement Pathway, Mannose-Binding Lectin ,Mannose-Binding Lectin ,Cells, Cultured - Abstract
To investigate the effect of mannose binding lectin (MBL) complement pathway on expression of transforming growth factor-beta1 (TGF-beta1) and NF-kappaB in cultured human renal glomerular endothelial cells (HRGECs) stimulated by high concentration of glucose.Human glomerular endothelial cells in culture were randomly divided into 5 groups according to different managements: normal concentration of glucose as controlled group, MBL + normal concentration of glucose group, high concentration of glucose, MBL + high glucose and MBL + high glucose + MBL blocker respectively. Flow cytometry was used to detect the depositions of MBL and C3 on the surfaces of HRGECs. Real-time PCR method was used to detect the mRNA levels of TGF-beta1. Human TGF-beta1 ELISA kit was used to detect the concentration of TGF-beta1 in supernatant fluid. ESMA was used to detect the activity of NF-kappaB in HRGECs.Compared with the normal glucose group and high glucose group, the depositions of MBL, C3 were apparently increased in MBL + high glucose group (P0.05). Expression of TGF-beta1 were significantly higher (P0.05) in MBL + high concentration of glucose groups than the normal glucose group and the high concentration of glucose group. Compared with the high glucose group, the activity of NF-kappaB in HRGECs was apparently increased in MBL + high glucose group, which could be significantly downregulated by MBL blocking antibody.High concentration of glucose can increase the expression of TGF-beta1 of cultured human glomerular endothelial cells. At the same time, high glucose together with MBL can up regulate the expression of TGF-beta1 and the activity of NF-kappaB in HRGECs. more...
- Published
- 2011
31. [Effect of high concentration of glucose on thickness of glycocalyx and expression of syndecan-1 and glypican-1 in cultured human renal glomerular endothelial cells]
- Author
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Hong-yu, Qiu, Wen-ring, Fan, Song-min, Huang, Fang, Liu, Wan-zin, Tang, and Chuan, Zuo
- Subjects
Glucose ,Glypicans ,Kidney Glomerulus ,Endothelial Cells ,Humans ,RNA, Messenger ,Syndecan-1 ,Glycocalyx ,Cells, Cultured - Abstract
To investigate the influence of high concentration of glucose on the thickness of Glycocalyx and expression of core protein Sydecan-1 and Glypican-1 in cultured human renal glomerular endothelial cells (HRGECs).HRGECs in culture were randomly divided into 3 groups, high concentration of glucose (30 mmol/L D-glucose, high glucose group), normal concentration of glucose as controlled group (5 mmol/L D-glucose+25 mmol/L mannitol, normal control group), and mannitol group (30 mmol/L mannitol) respectively. After 72 hours, confocal laser scanning microscopy (CLSM) was used to observe and characterize the fully hydrated glycoalyx of HRGECs. Real time quantitative PCR and Western blot were applied to detect the mRNA levels and protein expression of Syndecan-1 and Glypican-1, and the fluorescence microscope were used to observe the immunofluorescence change of Syndecan-1 and Glypican-1.Compared with normal control group, the thickness of Glycocalyx on the surface of HRGECs in high glucose group decreased to 36.8% (P0.05). Immunofluorescence shows the depositions of Syndecan-1 and Glypican-1 were weakened in high glucose group. The mRNA and protein expression of Syndecan-1 and Glypican-1 were significantly decreased (P0.05) compared with normal control group and mannitol group.High concentration of glucose can reduce thickness of Glycocalyx on the surface of human glomerular endothelial cell. At the same time, high glucose can decrease the expression of core protein Sydecan-1 and Glypican-1 of HRGECs. more...
- Published
- 2011
32. Neurotensin modulates pacemaker activity in interstitial cells of Cajal from the mouse small intestine
- Author
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Jun Lee, Cheol Ho Yeum, In Yeoub Chang, Dong Chuan Zuo, Seok Choi, Young Dae Kim, Pawan K Shahi, Jae Yeoul Jun, Man Yoo Kim, and Chan Guk Park
- Subjects
Male ,medicine.medical_specialty ,Thapsigargin ,Immunocytochemistry ,Enteroendocrine cell ,Calcium-Transporting ATPases ,Biology ,In Vitro Techniques ,Inhibitory postsynaptic potential ,Endoplasmic Reticulum ,complex mixtures ,digestive system ,Ion Channels ,Membrane Potentials ,symbols.namesake ,chemistry.chemical_compound ,Mice ,Internal medicine ,Intestine, Small ,medicine ,Animals ,Receptors, Neurotensin ,Patch clamp ,Molecular Biology ,Cells, Cultured ,Neurotensin ,Mice, Inbred BALB C ,digestive, oral, and skin physiology ,Sodium ,Muscle, Smooth ,Cell Biology ,General Medicine ,Articles ,Interstitial Cells of Cajal ,Small intestine ,Interstitial cell of Cajal ,Endocrinology ,medicine.anatomical_structure ,chemistry ,nervous system ,Type C Phospholipases ,symbols ,Calcium ,Female ,hormones, hormone substitutes, and hormone antagonists - Abstract
Neurotensin, a tridecapeptide localized in the gut to discrete enteroendocrine cells of the small bowel mucosa, is a hormone that plays an important role in gastrointestinal secretion, growth, and motility. Neurotensin has inhibitory and excitatory effects on peristaltic activity and produces contractile and relaxant responses in intestinal smooth muscle. Our objective in this study is to investigate the effects of neurotensin in small intestinal interstitial cells of Cajal (ICC) and elucidate the mechanism. To determine the electrophysiological effects of neurotensin on ICC, whole-cell patch clamp recordings were performed in cultured ICC from the small intestine. Exposure to neurotensin depolarized the membrane of pacemaker cells and produced tonic inward pacemaker currents. Only neurotensin receptor1 was identified when RT-PCR and immunocytochemistry were performed with mRNA isolated from small intestinal ICC and c-Kit positive cells. Neurotensin-induced tonic inward pacemaker currents were blocked by external Na+- free solution and in the presence of flufenamic acid, an inhibitor of non-selective cation channels. Furthermore, neurotensin-induced action is blocked either by treatment with U73122, a phospholipase C inhibitor, or thapsigargin, a Ca2+-ATPase inhibitor in ICC. We found that neurotensin increased spontaneous intracellular Ca2+ oscillations as seen with fluo4/AM recording. These results suggest that neurotensin modulates pacemaker currents via the activation of non-selective cation channels by intracellular Ca2+-release through neurotensin receptor1. more...
- Published
- 2010
33. [Effects of ginsenoside Rg1 on streptozocin-induced diabetic nephropathy in rats]
- Author
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Xiaofen, Ma, Xisheng, Xie, Chuan, Zuo, and Junming, Fan
- Subjects
Male ,Rats, Sprague-Dawley ,Transforming Growth Factor beta1 ,Random Allocation ,Ginsenosides ,Animals ,Diabetic Nephropathies ,RNA, Messenger ,Diabetes Mellitus, Experimental ,Drugs, Chinese Herbal ,Phytotherapy ,Rats - Abstract
This study sought to assess the effect of Ginsenoside Rg1 on streptozocin-induced diabetic nephropathy in rats and to unveil the underlying mechanism. Diabetic nephropathy (DN) was induced by intraperitoneal injection of streptozocin (STZ). Eight weeks after drug administration, the rats from each group were sacrificed. Serum creatine (Scr) and 24 hours urine protein, cross reaction protein (CRP) and tumor necrosis factor-alpha (TNF-alpha) were measured at the end of the study. The histological changes of renal interstitial tissues were observed by periodic acid-Schiff staining (PAS). Immunohistochemical method was used to examine the expression levels of ectodermal dysplasia (ED-1). The mRNA of transforming growth factor-beta1 (TGF-beta1) was measured by real-time PCR (RT-PCR), and the protein expression of TGF-beta1 was surveyed by Enzyme-Linked Immunosorbent Assay (ELISA). The renal pathological changes in DN rats given ginsenoside Rg1 treatment were ameliorated, and the expression levels of 24 h urine protein, serum creatinine, CRP, TNF-alpha, ED-1 and TGF-beta1 were significantly lower than those in the diabetic nephropathy group (P0.05). So, we reach a conclusion that, in the experiment, Ginsenoside Rg1 obviously reduced TGF-beta1 expression and the already-mentioned inflammatory reaction factors in the renal tissues and improved the renal pathological changes in DN rats. more...
- Published
- 2010
34. ChemInform Abstract: Highly Diastereo- and Enantioselective Direct Aldol Reactions by 4-tert-Butyldimethylsiloxy-Substituted Organocatalysts Derived from N-Prolylsulfonamides in Water
- Author
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Xiaoju Wu, Xiangkai Fu, Xiao‐chuan Zuo, Shao-dong Fu, and Shu‐peng Zhang
- Subjects
Aldol reaction ,Chemistry ,Organocatalysis ,Enantioselective synthesis ,Organic chemistry ,General Medicine - Published
- 2010
- Full Text
- View/download PDF
35. [Clinical efficacy of Corydalis composite combined with methotrexate in treating rheumatoid arthritis]
- Author
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Chuan, Zuo, Yong-tao, Chen, and Zhong-ming, Wang
- Subjects
Adult ,Arthritis, Rheumatoid ,Male ,Biological Products ,Methotrexate ,Treatment Outcome ,Corydalis ,Antirheumatic Agents ,Humans ,Drug Therapy, Combination ,Female ,Middle Aged - Abstract
To observe the clinical efficacy and safety of Corydalis composite (CDC) combined with methotrexate (MTX) in treating rheumatoid arthritis (RA).Seventy-six RA patients were randomly assigned to 2 groups, 37 in the treated group received the combined therapy, and the 39 received MTX treatment alone, all were treated for 12 weeks. Efficacy of treatment was evaluated adopting the standard of American College of Rheumatology (ACR), taking ACR20 as the chief criterion; ACR50, ACR70 as well as the clinical indexes and items in Health Account Questionnaire (HAQ) as the auxiliary criteria, including joint swelling index, joint tenderness index, holding power, morning stiffness time, resting pain, erythrocyte sedimentation rate (ESR), C-reactive protein. And the adverse reaction was recorded at the same time.After being treated for 4, 8 and 12 weeks, the ACR20 response rate reached 35.14%, 59.46% and 70.27% respectively in patients of the treated group, while that in the control group was 17.95%, 35.90% and 46.15% respectively, significant difference between groups was shown in the outcome of week 8 and 12 (P0.05). ACR50 and ACR70 improving rate at all the time points of observation were increased in the treated group, with the ACR50 improving rate at week 12 higher than that in the control group (43.24% vs. 20.51%, P0.05). As compared with the control group, the improvements in all the auxiliary criteria were more significant in the treated group (P0.05). The incidence of adverse reaction was less in the treated group than in the control group (32.43% vs. 56.41%, P0.05), particularly in term of the damage on liver (0 vs. 10.26%, P0.05).CDC combined with MTX is more effective than MTX alone in treating active RA with less adverse reaction. more...
- Published
- 2010
36. (-)-epigallocatechin gallate inhibits the pacemaker activity of interstitial cells of cajal of mouse small intestine
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Cheol Ho Yeum, Seok Choi, Soo Jin Choi, Jae Yeoul Jun, Pawan K Shahi, Pyung Jin Yoon, Dong Chuan Zuo, Shankar P. Parajuli, Kweon Young Kim, and Hyuk Jin Jang
- Subjects
Physiology ,Mouse Small Intestine ,Epigallocatechin gallate ,complex mixtures ,Glibenclamide ,symbols.namesake ,chemistry.chemical_compound ,Medicine ,Channel blocker ,heterocyclic compounds ,Pharmacology ,business.industry ,food and beverages ,Anatomy ,Gallate ,Molecular biology ,Small intestine ,Interstitial cell of Cajal ,medicine.anatomical_structure ,chemistry ,symbols ,Original Article ,sense organs ,business ,medicine.drug ,Guanylate cyclase - Abstract
The effects of (-)-epigallocatechin gallate (EGCG) on pacemaker activities of cultured interstitial cells of Cajal (ICC) from murine small intestine were investigated using whole-cell patch-clamp technique at 30 and Ca(2+) image analysis. ICC generated spontaneous pacemaker currents at a holding potential of -70 mV. The treatment of ICC with EGCG resulted in a dose-dependent decrease in the frequency and amplitude of pacemaker currents. SQ-22536, an adenylate cyclase inhibitor, and ODQ, a guanylate cyclase inhibitor, did not inhibit the effects of EGCG. EGCG-induced effects on pacemaker currents were not inhibited by glibenclamide, an ATP-sensitive K(+) channel blocker and TEA, a Ca(2+)-activated K(+) channel blocker. Also, we found that EGCG inhibited the spontaneous [Ca(2+)](i) oscillations in cultured ICC. In conclusion, EGCG inhibited the pacemaker activity of ICC and reduced [Ca(2+)](i) oscillations by cAMP-, cGMP-, ATP-sensitive K+ channel-independent manner. more...
- Published
- 2010
37. Low molecular weight heparin (LMWH) versus unfractionated heparin (UFH) for haemodialysis anticoagulation
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Xi Sheng Xie, Kun Yang, Jun-ming Fan, and Chuan Zuo
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medicine.medical_specialty ,medicine.drug_class ,business.industry ,Anesthesia ,medicine ,Low molecular weight heparin ,Heparin ,Intensive care medicine ,business ,medicine.drug - Published
- 2010
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- View/download PDF
38. FCSNV: A NEW VECTOR MODEL TO REPRESENT CHINESE DOCUMENTS
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Xueming Li, Jiapei Zhang, Chuan Zuo, and Minling Xing
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Computer science ,business.industry ,Vector (epidemiology) ,Pattern recognition ,Artificial intelligence ,business - Published
- 2009
- Full Text
- View/download PDF
39. [Effect of ginsenoside Rgl on the expression of TNF-alpha and MCP-1 in rats with diabetic nephropathy]
- Author
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Li-na, Zhang, Xi-sheng, Xie, Chuan, Zuo, and Jun-ming, Fan
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Male ,Ginsenosides ,Podocytes ,Tumor Necrosis Factor-alpha ,Panax ,Nephrons ,Streptozocin ,Diabetes Mellitus, Experimental ,Rats ,Rats, Sprague-Dawley ,Random Allocation ,Animals ,Diabetic Nephropathies ,RNA, Messenger ,Chemokine CCL2 - Abstract
To investigate the effects of Ginsenoside Rgl on proteinuria and the expression of monocyte chemotactic protein-1 (MCP-1) and tumor necrosis factor-alpha (TNF-alpha) in rats with diabetic nephropathy (DN).The DN rat model was established by injection of streptozotocin (STZ, 65 mg/kg) in abdominal cavity. Forty Sprague-Dawley male rats were randomly divided into 4 groups: normal group, DN group, Ginsenoside Rgl treatment group and Irbesartan treatment group. The blood glucose was monitored routinely. Twenty-four hours urine protein and serum creatine were measured the day before the rats were killed when the eight weeks of treatments had been completed. The renal pathological and podocyte changes were evaluated. Immunohistochemistry and enzyme-linked immunosorbent assay (ELISA) were performed to examine the protein expression levels of MCP-1 and TNF-alpha, respectively. The mRNA of TNF-alpha and MCP-1 were reverse transcribed and quantified by real-time PCR.The DN rats had increased volume of renal glomerulus, thickened basement membrane, and increased mesenterium mass, as well as some inflammatory cells in renal glomerulus. The number of potocyte decreased significantly in the DN group compared with the normal group (P0.01). Compared with the DN group, the basement membrane became thinning and the number of podocyte increased in the two treatment groups (P0.05). The rats in the DN group and the two treatment groups had significantly higher levels of twenty-four hour urine protein, serum creatine, serum glucose, serum MCP-1 and TNF-alpha than the normal rats (P0.05). The rats in the treatment groups had lower levels of twenty-four hours urine protein and serum creatine than the rats in the DN group (P0.05). But the serum glucose had little changes (P0.05). There was no difference between the two treatment groups. Immunohistochemisty, ELISA and real-time PCR results indicated that the expression levels of MCP-1 and TNF-alpha in the rats in the DN group and the two treatment groups were significantly higher than those in the normal group (P0.05). The rats in the treatment groups had lower levels of expression of MCP-1 and TNF-alpha than those in the DN group (P0.05). The correlation analysis indicated that the levels of MCP-1 and TNF-alpha were positively related to twenty-four hours urine protein (r=0.7802, 0.6963), glomerular sclerosis index (r=0.8296, 0.7413) and thickness of podocyte membrane (r=0.7678, 0.6701, P0.05).Ginsenoside Rgl reduces the expression of MCP-1 and TNF-alpha, repairs the pathological lesions of podocyte and nephron, and reduces the twenty-four hour urine protein rats with diabetic nephropathy. more...
- Published
- 2009
40. Hiding association rules based on relative-non-sensitive frequent itemsets
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Zhijun Liu, Xueming Li, and Chuan Zuo
- Subjects
Information sensitivity ,Information privacy ,Focus (computing) ,Distance measurement ,Association rule learning ,Computer science ,Data mining ,computer.software_genre ,Database transaction ,computer ,Information protection policy - Abstract
Association rules hiding algorithms often sanitize transactional databases for protecting sensitive information. Data modification is one of the most important sanitation approaches. However, the exist modification methods either focus on hiding sensitive rules only, or take measures to reduce the impact on non-sensitive rules from the whole database while hiding sensitive rules. In this paper, we propose a new algorithm which hides sensitive rules from the side of non-sensitive rules. It classifies the sensitive transactions by their degree of conflict. For the special group of transactions, a victim-item must satisfy: 1, in the sensitive rules; 2, not in the non-sensitive rules. Our algorithm selects different victim-items in different transactions that contain the same rule, which makes sure that removing the victim-items in the special group of transactions has no influence to non-sensitive rules. The experimental results show that our algorithm for sanitizing transactional database can achieve better results compared with others algorithms such as Naive, MinFIA, MaxFIA and IGA. In particular, our algorithm has the least impact on non-sensitive rules. more...
- Published
- 2009
- Full Text
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41. [Effect of Astragalus mongholicus on expression of transforming growth factor- beta1 in SD rats with unilateral ureteral occlusion]
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Chuan, Zuo, Xisheng, Xie, Yao, Deng, and Junming, Fan
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Male ,Transforming Growth Factor beta1 ,Kidney Tubules ,Gene Expression Regulation ,Animals ,Astragalus Plant ,RNA, Messenger ,Actins ,Drugs, Chinese Herbal ,Rats ,Ureteral Obstruction - Abstract
To study the effect of Astragalus mongholicus (AM) on the expression of transforming growth factor-beta1 (TGF-beta1) in SD rats with unilateral ureteral occlusion (UUO) and to elucidate the mechanisms underlying the renoprotective effects of AM.Fifty-four Sprague-Dawley rats were randomly divided into 4 groups: sham-operation group, the UUO group and AM treatment group. After administration of AM (10 g kg(-1) d(-1)) for 3, 7 and 14 days, the dynamic histological changes of renal interstitial tissues were observed and renal damage including tubular impairment and interstitial fibrosis were quantified on HE and Masson stained tissue sections. The expression of TGF-beta1 and alpha-smooth muscle actin (alpha-SMA) was measured by immunohistochemistry staining sections. The mRNA of TGF-beta1 and alpha-SMA were reverse transcribed and quantified by real-time PCR. The expression of TGF-beta1 protein were assessed by Western blot.Renal damage was exacerbated and the expression of alpha-SMA and TGF-beta1 were all significantly increased in UUO group compared with those of sham-operation group (P0.05) at each time point. Tubular impairment and interstitial fibrosis were alleviated, and up-regulations of expressions of TGF-beta1 and alpha-SMA were significantly suppressed by AM treatment (P0.05).AM can ameliorate renal interstitial fibrosis induced by UUO in vivo. The mechanisms of its antifibrotic effects might be related with the down-regulation of TGF-beta1 expression and suppression of tubular epithelial myofibroblast transdifferentiation in the progress of renal interstitial fibrosis. more...
- Published
- 2009
42. [Effect of Astragalus mongholicus on expression of hepatocyte growth factor in SD rats with unilateral ureteral obstruction]
- Author
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Chuan, Zuo, Hong-Yu, Qiu, Xi-Sheng, Xie, Yao, Deng, and Jun-Ming, Fan
- Subjects
Male ,Rats, Sprague-Dawley ,Random Allocation ,Nephrosclerosis ,Hepatocyte Growth Factor ,Animals ,RNA, Messenger ,Astragalus propinquus ,Proto-Oncogene Proteins c-met ,Actins ,Drugs, Chinese Herbal ,Rats ,Ureteral Obstruction - Abstract
To study the effect of Astragalus mongholicus (AM) on the expression of hepatocyte growth factor (HGF) in SD rats with unilateral ureteral obstruction (UUO) and to elucidate the mechanisms underlying the renoprotective effects of AM.Fifty four Sprague-Dawley rats were randomly divided into 3 groups: sham-operation group (SOR), UUO group (UUO) and UUO + AM group (AM). After administration of AM[10 g/ (kg x d)] for 3, 7 and 14 days,the histological changes of renal interstitial tissues were observed dynamically, and renal damage including tubular impairment and interstitial fibrosis were quantified on HE and Masson stained tissue sections. The protein expression of HGF and alpha-smooth muscle actin (alpha-SMA) was measured by immunohistochemistry. The mRNA expression of HGF and alpha-SMA were determined by real-time PCR. The expression of HGF and its receptor (C-met) were assessed by Western blot.Renal damage was exacerbated and the expression of alpha-SMA was significantly increased in UUO group compared with those of SOR group (P0.05) at each time point. HGF and C-met expression peaked at the 7th day after UUO and then decreased greatly. After AM intervention, tubular impairment and interstitial fibrosis were alleviated, up-regulations of alpha-SMA expressions was significantly suppressed, whileas the expression of HGF and C-met were significantly increased when compared with UUO group (P0. 05) at each time point.AM can ameliorate renal interstitial fibrosis induced by UUO in rats. The mechanisms of its antifibrotic effects may be related with the up-regulation of HGF and C-met expression, and the suppression of tubulo-epithelial mesenchymal transdifferentiation in renal intersitial progress. more...
- Published
- 2009
43. [Effect of Astragalus mongholicus on expression of connective tissue growth factor in kidney of SD rats with unilateral ureteral obstruction]
- Author
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Chuan, Zuo, Xi-sheng, Xie, Yao, Deng, and Jun-ming, Fan
- Subjects
Male ,Reverse Transcriptase Polymerase Chain Reaction ,Connective Tissue Growth Factor ,Astragalus Plant ,Kidney ,Immunohistochemistry ,Actins ,Rats ,Rats, Sprague-Dawley ,Disease Models, Animal ,Random Allocation ,Animals ,Kidney Diseases ,RNA, Messenger ,Drugs, Chinese Herbal ,Ureteral Obstruction - Abstract
To study the effect of Astrangalus mongholicus (AM) on the expression of Connective Tissue Growth Factor (CTGF) in SD rats with Unilateral Ureteral Obstruction (UUO) and elucidate the mechanism underlying the renorotective effects of AM.36 Sprague-Dawley rats were randomly divided into 3 groups: sham-operation group (Sham), UUO group (UUO) and UUO + AM group (AM). After administration of AM (10 g/kg x d) for 7 and 14 days, the dynamic histological changes of renal interstitial tissues were observed and renal damage including tubular impairment and interstitial fibrosis were quantified on HE and Masson stained tissue sections. The expressions of CTGF and alpha-smooth muscle actin (alpha-SMA) were measured by immunohistochemistry staining sections. The mRNA of CTGF and alpha-SMA were reversely transcribed and quantified to real-time PCR. The expression of CTGF protein was assessed by Western blot.Renal damage was exacerbated and the expressions of alpha-SMA and CTGF significantly increased in UUO group compared with those of Sham group (P0.05) at each time point. Tubular impairment and interstitial fibrosis were alleviated, and up-regulations of expressions of CTGF and alpha-SMA were significantly depressed by AM treatment (P0.05).AM can ameliorate renal interstitial fibrosis induced by UUO in rats. The mechanism of its antifibrotic effects may be related to the down-regulation of CTGF expression, following suppression of tubulo-epithelial mesenchymal transdifferentation in renal intersitial progress. more...
- Published
- 2008
44. [Regulative effect of Astragalus mongholicus on hepatocyte growth factor and transforming growth factor-beta1 in rats with unilateral ureteral obstruction]
- Author
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Chuan, Zuo, Xisheng, Xie, Yao, Deng, and Junming, Fan
- Subjects
Male ,Rats, Sprague-Dawley ,Transforming Growth Factor beta1 ,Random Allocation ,Hepatocyte Growth Factor ,Animals ,Kidney Diseases ,RNA, Messenger ,Astragalus propinquus ,Fibrosis ,Drugs, Chinese Herbal ,Rats ,Ureteral Obstruction - Abstract
To study the effect of Astragalus mongholicus (AM) on renal interstitial fibrosis caused by unilateral ureteral obstruction (UUO) in rats in order to elucidate the mechanisms underlying the renoprotective effects of AM.54 Sprague-Dawley rats were randomly divided into 3 groups: sham-operation group (Sham), the UUO group(UUO) and UUO + AM group (AM). The rats were treated at the doses of 10 g/(kg X d) AM for 3, 7 and 14 days, the dynamic histological changes of renal interstitial tissues were observed and renal damage including tubular impairment and interstitial fibrosis were quantified on HE and Masson stained tissue sections. The expressions of transforming growth factor-beta1 (TGF-beta1) and hepatocyte growth factor (HGF) were measured by immunohistochemistry staining sections. The mRNA of HGF and TGF-beta1 were reverse transcribed and quantified by real-time PCR. The expressions of HGF and TGF-beta1 protein were assessed by Western blot.Renal damages were exacerbated and the expressions of TGF-beta1 were significantly increased in UUO group in comparison with those of Sham group (P0.05) at each time point. HGF expression increased initially and peaked at the day of 7 after UUO and then decreased greatly. The tubular impairment and interstitial fibrosis were alleviated, up-regulations of expressions of TGF-beta1 were significantly suppressed, while expressions of HGF were significantly increased in UUO + AM group in comparison with UUO group (P0.05).AM could ameliorate renal interstitial fibrosis induced by UUO in rats. The mechanisms of antifibrotic effects of AM may be associated with up-regulation of HGF expression and down-regulation of TGF-beta1 expression in renal intersitial progress. more...
- Published
- 2008
45. Influence of ginsenoside Rg1, a panaxatriol saponin from Panax notoginseng, on renal fibrosis in rats with unilateral ureteral obstruction
- Author
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Jun-ming Fan, Chuan Zuo, Zi Li, Heng-cuang Liu, Yao Deng, Man Yang, and Xi-sheng Xie
- Subjects
Male ,medicine.medical_specialty ,Ginsenosides ,medicine.medical_treatment ,Panax notoginseng ,Smad2 Protein ,General Biochemistry, Genetics and Molecular Biology ,Collagen Type I ,Rats, Sprague-Dawley ,Thrombospondin 1 ,Transforming Growth Factor beta1 ,chemistry.chemical_compound ,Random Allocation ,Internal medicine ,medicine ,Renal fibrosis ,Animals ,RNA, Messenger ,General Pharmacology, Toxicology and Pharmaceutics ,Messenger RNA ,Kidney ,General Veterinary ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,Panaxatriol ,General Medicine ,medicine.disease ,biology.organism_classification ,Cadherins ,Immunohistochemistry ,Actins ,Fibronectins ,Rats ,Biomedicine ,medicine.anatomical_structure ,Cytokine ,Endocrinology ,chemistry ,Nephritis, Interstitial ,Nephritis ,Ureteral Obstruction - Abstract
Total saponins of Panax notoginseng (PNS) have been shown to ameliorate renal interstitial fibrosis. Ginsenoside Rg1, a panaxatriol saponin, is one of the major active molecules from PNS. The present study was undertaken to investigate the effect of ginsenoside Rg1 on renal fibrosis in rats with unilateral ureteral obstruction (UUO). The rats were randomly divided into 3 groups: sham-operation (n=15), UUO (n=15) and UUO with ginsenoside Rg1 treatment (n=15, 50 mg per kg body weight, intraperitoneally (i.p.) injected). The rats were sacrificed on Days 7 and 14 after the surgery. Histological examination demonstrated that ginsenoside Rg1 significantly inhibited interstitial fibrosis including tubular injury as well as collagen deposition. alpha-smooth muscle actin (alpha-SMA) and E-cadherin are two markers of tubular epithelial-myofibroblast transition (TEMT). Interestingly, ginsenoside Rg1 notably decreased alpha-SMA expression and simultaneously enhanced E-cadherin expression. The messenger RNA (mRNA) of transforming growth factor-beta1 (TGF-beta1), a key mediator to regulate TEMT, in the obstructed kidney increased dramatically, but was found to decrease significantly after administration of ginsenoside Rg1. Further study showed that ginsenoside Rg1 considerably decreased the levels of both active TGF-beta1 and phosphorylated Smad2 (pSmad2). Moreover, ginsenoside Rg1 substantially suppressed the expression of thrombospondin-1 (TSP-1), a cytokine which can promote the transcription of TGF-beta1 mRNA and the activation of latent TGF-beta1. These results suggest that ginsenoside Rg1 inhibits renal interstitial fibrosis in rats with UUO. The mechanism might be partly related to the blocking of TEMT via suppressing the expression of TSP-1. more...
- Published
- 2008
46. [The effect of ginsenoside Rg1 on the renal interstitial fibrosis of UUO rat]
- Author
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Xi-Sheng, Xie, Heng-Chuan, Liu, Chuan, Zuo, Yao, Deng, and Jun-Ming, Fan
- Subjects
Male ,Ginsenosides ,Reverse Transcriptase Polymerase Chain Reaction ,Blotting, Western ,Gene Expression ,Kidney ,Fibrosis ,Immunohistochemistry ,Rats ,Rats, Sprague-Dawley ,Random Allocation ,Transforming Growth Factor beta ,Animals ,Nephritis, Interstitial ,Ureteral Obstruction - Abstract
To study the effect of ginsenoside Rg1 on the renal interstitial fibrosis caused by unilateral ureteral obstruction (UUO) of rats.80 healthy Sprague-Dawley rats were randomly divided into 4 groups: the UUO group (UUO), sham-operation group (SOR), ginsenoside Rg1 group (Rg1) and losartan group (ARB). From the first day after initial UUO, ARB group was intragastrically administrated with losartan 20 mg/(kg x d). UUO, Rg1 and SOR groups were intragastrically administrated with identical volume of normal saline. Rg1 group was intraperitoneally injected with ginsenoside Rg1 50 mg/(kg x d). UUO, ARB and SOR groups were intraperitoneally injected with identical volume of normal saline. At day 3, 7, 14 after UUO, 6 rats selected randomly from each group were killed. The dynamic histological changes of renal interstitial tissues were observed by HE, Masson and PAS staining. The mRNA of transforming growth factor-beta1 (TGF-beta1) was quantified by real-time PCR. The protein levels of TGF-beta1 expression were assessed by Western blot and immunohistochemical method respectively.In UUO kidneys, the interstitial fibrosis including tubular atrophy, loss and dilation, inflammatory cell infiltration and interstitial matrix deposition was prominent. However, these morphological changes were notably reduced in Rg1 and ARB groups, and there was no significant difference between the two groups (P0.05). TGF-beta1 mRNA and protein expression were increased dramatically for UUO group at postoperative day 7 and 14 (P0.05). TGF-beta1 expression in Rg1 and ARB groups were significantly lower than that in UUO group (P0.05).Ginsenoside Rg1 can evidently inhibit UUO-induced renal interstitial fibrosis in rat, which may be related to the down regulation of TGF-beta1 expression. more...
- Published
- 2008
47. [Relationship between cardiovascular complication and inflammation, oxidative stress of patient with maintenance hemodialysis]
- Author
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Chuan, Zuo, Hong-yu, Qiu, Zi, Li, Xi-sheng, Xie, and Jun-ming, Fan
- Subjects
Adult ,Inflammation ,Male ,Glutathione Peroxidase ,Superoxide Dismutase ,Middle Aged ,Oxidative Stress ,C-Reactive Protein ,Logistic Models ,Cardiovascular Diseases ,Renal Dialysis ,Risk Factors ,Malondialdehyde ,Humans ,Female - Abstract
To evaluate and analyze relationships between states of inflammation, oxidative stress and complicatedly cardiovascular diseases in patients with maintenance hemodialysis (MHD).The analytical method for xanthine oxidase was used to measure the serum superoxide dismutase (SOD), spectrophotometry was used to measure the serum MDA, GSHPx. The immune turbidimetry was used to measure the serum levels of C-reactive protein (CRP) in patients with chronic renal failure.Compared with normal control group, the uremia groups had lower serum GSHPx, SOD and ALB levels, but higher CRP and MDA levels (P0.01). The MHD patients exhibited more serious disturbance of inflammation and oxidative stress which were aggravated as MHD time extended. CRP and MDA were significantly increased but GSHPx,SOD and ALB decreased in patients with cardiovascular events compared with the patients without them (P0.01). The results of Pearson correlation analysis showed: CRP was correlated with MDA positively (r = 0.740, P0.01), while with GSHPx, SOD, ALB negatively (r = -0.684, -0.688, -0.924, P0.01). ALB was correlated with GSHPx, SOD positively (r = 0.744, 0.728, P0.01), while with MDA negatively (r = -0 779, P0.01). The results of Logistic regression analysis showed that CRP contributed to the predictability of incidence of CVD (regression coefficient b = -1.054, Wald chi-square = 5.251, OR = 0.348, P0.05).MHD patients are in the states of inflammation and oxidative stress which are aggravated with the prolonging of hemodialysis time. Inflammation and oxidative stress may be the risk factors for occurrence of cardiovascular events. more...
- Published
- 2008
48. Ginsenoside Rg1, a major active component isolated from Panax notoginseng, restrains tubular epithelial to myofibroblast transition in vitro
- Author
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Heng-Chuan Liu, Jun-ming Fan, Man Yang, Hui-Juan Li, Chuan Zuo, and Xi-Sheng Xie
- Subjects
Ginsenosides ,Panax notoginseng ,Plant Roots ,Collagen Type I ,Epithelium ,Cell Line ,Extracellular matrix ,Transforming Growth Factor beta1 ,chemistry.chemical_compound ,Western blot ,Drug Discovery ,Gene expression ,medicine ,Animals ,RNA, Messenger ,Pharmacology ,Mitogen-Activated Protein Kinase 3 ,biology ,medicine.diagnostic_test ,Plant Extracts ,Cell Differentiation ,Epithelial Cells ,Fibroblasts ,biology.organism_classification ,Cadherins ,Molecular biology ,Fibrosis ,Actins ,Fibronectins ,Rats ,Fibronectin ,Kidney Tubules ,Biochemistry ,chemistry ,Ginsenoside ,biology.protein ,Myofibroblast ,Transforming growth factor - Abstract
The medicinal herb, Panax notoginseng, has been used for thousands of years in traditional Chinese medicine and possesses anti-fibrosis properties. Epithelial-myofibroblast transition (EMT) plays an important role in renal tubulointerstitial fibrosis. The present study was designed to examine whether ginsenoside Rg1, a major active component isolated from Panax notoginseng, has an ability to block this phenotypic transition in rat renal tubular epithelial cells (NRK-52E) induced by transforming growth factor-beta1 (TGF-beta1). The morphology of tubular epithelial-myofibroblast transition was observed through light microscope and transmission electron microscopy. alpha-SMA and E-cadherin are two markers of tubular epithelial-myofibroblast transition, their protein expressions were assessed by immunohistochemistry and western blot analysis. Gene expression of alpha-SMA as well as the two major extracellular matrix components collagen I and fibronectin was measured by real-time PCR analysis. Enzyme-linked immunosorbent assay was used to quantitatively detect collagen I and fibronectin in the supernatant. Our results revealed that ginsenoside Rg1 obviously blocked morphologic transformation in NRK-52E induced by TGF-beta1. Meanwhile, ginsenoside Rg1 inhibited the expression of alpha-SMA and the loss of E-cadherin, subsequently decreased the levels of collagen I and fibronectin in a dose-dependent manner. In addition, western blot analysis indicated that ginsenoside Rg1 inhibited the expression of P-ERK1/2 in NRK-52E induced by TGF-beta1. These results suggest that ginsenoside Rg1 can restrain the process of EMT maybe via suppressing the expression of P-ERK1/2 in vitro. more...
- Published
- 2008
49. Design and research of zoom optic system applied in micro-processing using 248nm KrF excimer laser
- Author
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Xun Liu, Tao Chen, and Tie-chuan Zuo
- Subjects
Zoom lens ,Optics ,Materials science ,Digital zoom ,business.industry ,Focal length ,Field of view ,Image plane ,Zoom ,business ,Zemax ,Afocal system - Abstract
The content of this paper is a design of zoom optic system applied in 248nm KrF excimer laser micro-processing. Due to the characteristic of KrF laser and the applied requests of micro-processing, it was determined that the maximal field of view is 1.2°, the optical material is fused quartz (JGS1) and the value of working distance is greater than 30mm. On the premise that the system has stable image surfaces and good imaging qualities under each different focal length, the goals of design include the continuous zoom ratio Γ=1~3.5 and the minimal magnification M≈0.1. In addition, the mechanically compensated method was employed to compensate image surface, so as not to change the position of the image plane in the whole range of focal lengths or magnification. The zoom optical system was composed of three parts: the front fixed group, the zoom group and the compensated group. After perfect optical calculation, the correlation of zoom ratio and focal length was obtained. The maximum of focal length is 129.2308mm and minimum is 39.92926mm when M=0.1. For continuous improvement of imaging quality, the initial configuration has been optimized by making use of ZEMAX. The result of design can achieve the goal of zoom ratio. During the zoom process, the total length of system is about 290.7065mm with the deviation Δ=±0.0403mm and the minimum of working distance is 59.3094mm. The whole system has good imaging quality and effective etching capability. more...
- Published
- 2007
- Full Text
- View/download PDF
50. New ideas for teaching electrocardiogram interpretation and improving classroom teaching content
- Author
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Rui Zeng, Chun-Yu Tan, Chuan Zuo, Pan-Wen Tian, Pu Kuang, Qin Wang, and Rongzheng Yue
- Subjects
Classroom teaching ,Letter ,Computer science ,Teaching method ,media_common.quotation_subject ,Interpretation (philosophy) ,Bioinformatics ,Education ,Subject matter ,Innovative teaching ,Syllabus ,Basic knowledge ,Reading (process) ,ComputingMilieux_COMPUTERSANDEDUCATION ,Mathematics education ,ComputerSystemsOrganization_SPECIAL-PURPOSEANDAPPLICATION-BASEDSYSTEMS ,Advances in Medical Education and Practice ,media_common - Abstract
Rui Zeng,1 Rong-Zheng Yue,2 Chun-Yu Tan,3 Qin Wang,4 Pu Kuang,5 Pan-Wen Tian,6 Chuan Zuo3 1Department of Cardiovascular Diseases, 2Department of Nephrology, 3Department of Rheumatology and Immunology, 4Department of Endocrinology, 5Department of Hematology, 6Department of Respiratory Diseases, West China Hospital, School of Clinic Medicine, Sichuan University, Chengdu, People’s Republic of China Background: Interpreting an electrocardiogram (ECG) is not only one of the most important parts of diagnostics but also one of the most difficult areas to teach. Owing to the abstract nature of the basic theoretical knowledge of the ECG, its scattered characteristics, and tedious and difficult-to-remember subject matter, teaching how to interpret ECGs is as difficult for teachers to teach as it is for students to learn. In order to enable medical students to master basic knowledge of ECG interpretation skills in a limited teaching time, we modified the content used for traditional ECG teaching and now propose a new ECG teaching method called the “graphics-sequence memory method.” Methods: A prospective randomized controlled study was designed to measure the actual effectiveness of ECG learning by students. Two hundred students were randomly placed under a traditional teaching group and an innovative teaching group, with 100 participants in each group. The teachers in the traditional teaching group utilized the traditional teaching outline, whereas the teachers in the innovative teaching group received training in line with the proposed teaching method and syllabus. All the students took an examination in the final semester by analyzing 20 ECGs from real clinical cases and submitted their ECG reports. Results: The average ECG reading time was 32 minutes for the traditional teaching group and 18 minutes for the innovative teaching group. The average ECG accuracy results were 43% for the traditional teaching group and 77% for the innovative teaching group. Conclusion: Learning to accurately interpret ECGs is an important skill in the cardiac discipline, but the ECG’s mechanisms are intricate and the content is scattered. Textbooks tend to make the students feel confused owing to the restrictions of the length and the format of the syllabi, apart from many other limitations. The graphics-sequence memory method was found to be a useful method for ECG teaching. Keywords: new ideas, ECG, classroom teaching content, graphics-sequence memory more...
- Published
- 2015
- Full Text
- View/download PDF
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