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Supplemental material, sj-docx-1-onc-10.1177_11795549221111713 for Impact of Tumor Location on Survival in Patients With Colorectal Cancer: A Retrospective Cohort Study Based on Taiwan’s Cancer Registry Database by Shou-Chun Yu, Kuang-Ming Liao, Chia-Lin Chou, Yu-Feng Tian, Jhi-Joung Wang, Chung-Han Ho and Yow-Ling Shiue in Clinical Medicine Insights: Oncology

Additional file 1: Fig. S1. BRCA1 and BRCA2 expression in NPC cells treated with O6BG. a HONE-1 and b TW01 cells were treated with indicated concentrations of O6BG for 8 h. The IC50 concentration of O6BG in both HONE-1 and TW01 cells was 120 μM. Cell lysates were subjected to Western blot analyses after the indicated treatment. Fold changes in protein levels listed under each blot were normalized to the levels of the actin control. Representative results of at least three independent experiments are shown. Fig. S2. MGMT interacted with BRCA2 in NPC cells treated with CDDP. After treatment with or without 10 μM CDDP for 8 h, the protein lysates of (A) HONE-1 and b TW01 cells were subjected to Co-IP analyses with 1 μg/mL of anti-MGMT antibodies, followed by Western blot analyses. Representative results of at least three independent experiments are shown. Fig. S3. MGMT mediated the cytotoxicity of PARP inhibitor in NPC cells. The a survival rates, b percentages of apoptotic cells, and c γ-H2AX expression of TW01 cells were examined using a clonogenic assay, annexin V staining, and Western blot analyses. TW01 cells transfected with scrambled or MGMT-targeted siRNA were treated with olaparib for 24 h. Representative histograms indicated the percentages of colony formation and apoptotic cells (annexin V–positive cells). Experiments were conducted at least three times. Bar values are presented as mean ± SD. *P

In this paper, we describe the design and implementation of a synchronous EFL computer assisted English writing environment. In addition to supporting the basic writing function requirements, two novel mechanisms, namely, (1) synchronous text co-editing, and (2) voice delivery, have been designed to provide fundamental capabilities over the Internet. As a result, the designed system exploits the integration of computers and networking capabilities with linguistic and pedagogical principles crucial to web-based language learning. The system integrates CMC tools with database technology for the specific purpose of archiving the communications between tutors and students. Since the platform offers a bank of comments that are frequently used in these online tutorials, the system can store and tabulate each token instance when a comment is used. This database then offers instant cumulative profiles into tutor-learner interaction and into the common language errors or difficulties uncovered in the tutorials. Such an archive supports research into language learning difficulties and into patterns of tutor-learner interaction. This data is valuable in the assessment of pedagogical effectiveness and in the development of online tutorial materials that meet the attested needs of learners.