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Supplemental material, sj-docx-1-imj-10.1177_10815589221150643 for Clinical significance of anti-rheumatoid arthritis 33 antibody in patients with systemic lupus erythematosus by Wen-Yi Lu, Xiao-Ping Hong, Jing-Yi Xie, Cui-Lian Liu, Cui-Hong Chen, Qin-Huang, Bao-Dong Sun, Dong-Zhou Liu and Yu-Lan Chen in Journal of Investigative Medicine

Supplemental material, sj-docx-1-imj-10.1177_10815589221150643 for Clinical significance of anti-rheumatoid arthritis 33 antibody in patients with systemic lupus erythematosus by Wen-Yi Lu, Xiao-Ping Hong, Jing-Yi Xie, Cui-Lian Liu, Cui-Hong Chen, Qin-Huang, Bao-Dong Sun, Dong-Zhou Liu and Yu-Lan Chen in Journal of Investigative Medicine

A flowchart of HBV reactivation occurring in patients with RA with CHB showing antiviral prophylaxis, HBV reactivation, and hepatitis flare in these patients during one-year follow up. CHB: chronic HBV infection; HBV: hepatitis B virus; RA: rheumatoid arthritis. (PDF 33 kb)

Dynamic disease activity indicators in all patients with RA during 1-year follow-upâ . (PDF 71Â kb)

The other clinical responses between the CHB group and the non-CHB group. Comparison of the other clinical responses at each visit, including ACR70, PrGA, Pain VAS, HAQ, ESR, and serum MMP-3 levels. ACR: American College of Rheumatology; CHB: chronic HBV infection; ESR: erythrocyte sedimentation rate; HAQ: Stanford Health Assessment Questionnaire; MMP-3: matrix metalloproteinase-3; Pain VAS: pain visual analog scale; PrGA: provider global assessment of disease activity. (PDF 1468 kb)

To reduce the structural weight and volume and satisfy an optimal balance of performance constraints, a study using a porous rectangular foldable board and structurally improved trolley model was conducted, and motion simulations were performed to derive the static and transient structural properties. The simulations revealed that the fatigue lifetimes of both the main structure and the porous boards were in a high-standard-value range, whereas the transient analysis indicated that both the directional deformation and equivalent stress exhibited minimal local values. For porous boards simply supported only on two sides, the trolley had a much smaller collapsed volume than other types of trolley. The static analysis indicated that this trolley prototype had a good safety coefficient and long fatigue lifetime. The transient analysis showed that the porous area had a higher strength and bending load than the non-porous area. The overall transient response was close to that of a steady-state, showing a negligible effect on the structure.tc.

Background In vivo electroporation has been successfully used for the introduction of DNA, RNA, oligonucleotides, and proteins into cells for experimental and therapeutic purposes. The authors evaluated the efficacy of electroporation-mediated c-Fos antibody therapy for neuropathic pain in vitro and in vivo. Methods First, the authors studied the inhibitory effects of intrathecal c-Fos antibody electroporation on the activating protein (AP-1) promoter activity in cultured spinal neuronal cells transfected with p-AP-Luc plasmid and activated with 100 microm glutamate. The inhibitory effect of c-Fos antibody electroporation in the regulation of AP-1 promoter activity was assessed according to the relative luciferase activity. Second, rats with chronic constrictive injury underwent electroporation treatment for neuropathic pain using c-Fos antibody. Thermal nociceptive thresholds were measured before chronic constrictive injury and then on even-numbered days, up to and including day 14, to assess and compare the therapeutic effects of intrathecal electroporation. The time course was assessed by Western blot analysis and by immunohistochemical analysis. Pronociceptive gene expression was measured by assessing prodynorphin mRNA and dynorphin peptides on days 2 and 10 after intrathecal c-Fos electroporation. Results Cotransfection of c-Fos antibody significantly decreased glutamate-induced AP-1 activity. Intrathecal electrotransfer of c-Fos antibody attenuated spinal dynorphin levels, as manifested by significantly elevated pain thresholds in the chronic constrictive injury-affected limbs. Conclusion This study shows that transfer of antibody into rat spinal cords by intrathecal electroporation is a useful method to study the function of endogenous factors of spinal-related disorders.