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ObjectiveInfantile hemangioma (IH), the most common infantile vascular neoplasm, is uniquely characterized by rapid proliferation followed by slow spontaneous involution lasting for years. In IH lesions, perivascular cells are the most dynamic cell subset during the transition from the proliferation phase to the involution phase, and we aimed to systematically study this kind of cell.Methods and resultsCD146-selective microbeads were used to isolate IH-derived mural-like cells (HemMCs). Mesenchymal markers of HemMCs were detected by flow cytometry, and the multilineage differentiation potential of HemMCs was detected by specific staining after conditioned culture. CD146-selected nonendothelial cells from IH samples showed characteristics of mesenchymal stem cells with distinct angiogenesis-promoting effects detected by transcriptome sequencing. HemMCs spontaneously differentiated into adipocytes 2 weeks after implantation into immunodeficient mice, and almost all HemMCs had differentiated into adipocytes within 4 weeks. HemMCs could not be induced to differentiate into endothelial cells in vitro. However, 2 weeks after implantation in vivo, HemMCs in combination with human umbilical vein endothelial cells (HUVECs) formed GLUT1+ IH-like blood vessels, which spontaneously involuted into adipose tissue 4 weeks after implantation.ConclusionsIn conclusion, we identified a specific cell subset that not only showed behavior consistent with the evolution of IH but also recapitulated the unique course of IH. Thus, we speculate that proangiogenic HemMCs may be a potential target for the construction of hemangioma animal models and the study of IH pathogenesis.

Previously most of the applications of targeting components have been based on the enhanced permeability and retention effect achieved using folic acid, which consider the side effects of the targeting components to some extent. Herein, we report a new strategy to decorate the surface of MOFs using a pemetrexed (MTA) targeting molecule, affording a new drug delivery system of ALA@UIO-66-NH-FAM/MTA (ALA = 5-amino-levulinic acid and FAM = 5-carboxyfluorescein). The confocal microscopy and flow cytometry results showed that ALA@UIO-66-NH-FAM/MTA presented a better targeting effect compared to ALA@UIO-66-NH-FAM/FA (FA = folic acid) and indicated a gradually increasing tendency of the targeting effect with the increasing expression of folate receptors on the tumor cell cytomembrane. Furthermore, the cytotoxicity experiment indicates that the combination of chemotherapy and photodynamic therapy is a more effective therapy model than single chemotherapy and photodynamic therapy. This work demonstrates the first attempt at folic acid antagonist (MTA) modification for NMOFs, providing a new concept for the design of MOFs with folate receptor targeting capacity for clinical applications.

With the access of small hydro-power stations (SHS), the distribution system presents a multiple power supply pattern, which has a negative impact on the original local feeder automation (LFA) which is suitable for single side power supply system. In view of this effect, the phase to phase short-circuit fault (PPF) and single-phase grounding fault (SPGF) are treated differently, and an improved LFA method (IMLFA) considering SHS’s access based on low-frequency load shedding (LFLS) and high-frequency generator tripping (HFGT) is proposed. In case of SPGF, the section switch (SSW) and the outgoing circuit breaker of the substation shall be opened in sequence, and the isolated network (IN) generated in the process can be operated stably by cooperating with LFLS or HFGT while the fault is preliminary isolated. The SSW and the breaker are added with the function of no-voltage detection and synchronization detection. In case of instantaneous fault, the power supply in the fault area can be restored after a series of operations, and the IN can be connected to the grid. If it is a permanent fault, the section switches (SSWs) around the fault area will be locked to close in order to isolate the fault effectively, and the IN, not separated by the fault area from the grid, can be connected to the grid. Analysis and simulation show that the method is correct and feasible.