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Additional file 7:Figure S6. Metabolic profiling of irradiated and 6 h in 2 leaves with control untreated plants during 1 and 6 h. As a control, samples from fully irradiated leaves for 4 h (UV-B), and control untreated leaves (NI) are included. CA/PE: comparison of metabolite levels in leaves covered with a plastic that allows UV-B transmittance (CA) vs. levels in leaves covered with a plastic sheath that absorbs UV-B (PE, see Material and methods); PE UV-B/C: comparison of metabolites from PE-covered leaves in plants exposed to UV-B to those from PE-covered leaves in non-irradiated plants; UV-B/CA: metabolite level comparison in leaves that are directly UV-B-irradiated vs. levels in leaves covered with a plastic that allows UV-B transmittance (CA). Statistical analysis was done using one way ANOVA; statistically significant differences are labeled with * (α = 0.05). (PDF 577 KB)

Additional file 8:Figure S7. Metabolic profiling of irradiated and shielded leaves with varying canopy exposure to UV-B radiation. As a control, samples from fully UV-B-irradiated leaves for 4 h (UV-B), and control untreated leaves (C) are included. Statistical analysis was done using one way ANOVA; statistically significant differences are labeled with letters a and b (α = 0.05). (PDF 753 KB)

Additional file 1:Figure S1. Classification of UV-B-regulated genes identified by microarrays based on their putative function in fully UV-B-irradiated plants (WPI). (a and b) transcripts that are up (a) and down (b) regulated in fully UV-B-irradiated plants; (c and d) transcripts that are up (c) and down (d) regulated only in fully UV-B-irradiated plants and not when plants are only irradiated in 1, 2 or 3 leaves per plant. Classification was done for the UV-B-regulated transcripts that are changed at least 2-fold (p < 0.05). (PDF 253 KB)

Authors’ original file for figure 9

Additional file of Transcriptomic, proteomic and metabolomic analysis of UV-B signaling in maize

Additional file 4:Figure S3. Venn diagrams comparing transcriptome changes in leaves that were covered with a plastic sheath that absorbs UV-B. Only two adult leaves per plant were irradiated over a time course of 1, 2, 4, and 6 h. Up-regulated genes are in red, down-regulated genes are in green. (a) Intersection of genes differentially expressed in irradiated leaves; (b) Intersection of genes differentially expressed in shielded leaves; (c) Intersection of genes differentially expressed in immature ears. Each sample was compared to plants under control conditions in the absence of UV-B (NI). Transcripts showing changes higher than 2-fold (p < 0.05) were included in the classification. (PDF 289 KB)

Additional file 3:Figure S2. (a) Venn diagrams comparing transcriptome changes in shielded leaves that were irradiated in the absence of UV-B. Up-regulated genes are in red, down-regulated genes are in green. (PDF 245 KB)

Authors’ original file for figure 7